FastQCFastQC Report
Tue 31 May 2016
SRR1051261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051261_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7581462
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99522313.127059134504664No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1767652.3315423858881044No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010511.3328695705392972No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295220.38939719014617497No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT278980.36797651956838934No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256720.33861542800056244No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212330.2800647157500757No Hit
AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125740.1658519161607616No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTAAAAAAAAAA99210.13085866551860315No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT96720.12757433856425054No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT91320.12045170179577501No Hit
GGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90130.11888208369309244No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTAAAAAAAAA84780.11182539726506575No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81630.10767052581678836No Hit
AATTCGAGGCGGTACAGGTGTTCTCCCGTATTGTTGACATGCCAGCGGGT76320.10066659966112078No Hit
GGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75990.10023132741415837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG283250.094.284011
TCGACGG285600.093.6929862
CGACGGC310200.086.625753
GACGGCG327400.082.262384
ACGGCGC327850.082.191865
CGGCGCG344000.078.305526
ATCTCGA160600.064.532991
ATATCTC46350.061.103791
TATCTCG46950.060.8085172
TCTCGAG184450.056.1182482
CTCGAGG212700.048.773933
TCGAGGG213600.048.6344954
CGAGGGC231250.044.921915
CGAGGCG19300.044.054165
ATTCCCC40150.043.776572
GGCGCGC683450.043.013517
AATTCGA36500.042.9628141
CGCGCCG687950.042.683829
GCGCGCC696850.042.2000248
GCGGTAC21650.039.4916959
Produced by FastQC (version 0.11.5)