##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051261_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7581462 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.945195135186328 33.0 31.0 34.0 30.0 34.0 2 32.02762646571334 33.0 31.0 34.0 30.0 34.0 3 32.158242565879775 33.0 31.0 34.0 30.0 34.0 4 36.0678778050988 37.0 35.0 37.0 35.0 37.0 5 36.17064953962706 37.0 35.0 37.0 35.0 37.0 6 36.19864572294895 37.0 36.0 37.0 35.0 37.0 7 36.22424091817647 37.0 36.0 37.0 35.0 37.0 8 36.21815106373942 37.0 37.0 37.0 35.0 37.0 9 37.97249607001921 39.0 38.0 39.0 35.0 39.0 10-11 37.91742878880089 39.0 38.0 39.0 35.0 39.0 12-13 37.89024194805698 39.0 38.0 39.0 35.0 39.0 14-15 39.26718303145225 40.0 39.0 41.0 36.0 41.0 16-17 39.02161172871406 40.0 38.5 41.0 36.0 41.0 18-19 38.502197465343755 40.0 37.0 41.0 35.0 41.0 20-21 38.41796595432385 40.0 36.5 41.0 35.0 41.0 22-23 38.33597431471661 40.0 36.0 41.0 35.0 41.0 24-25 38.22201700674619 40.0 35.5 41.0 35.0 41.0 26-27 37.966329317485204 39.5 35.0 41.0 34.5 41.0 28-29 37.88398748157017 39.0 35.0 41.0 34.5 41.0 30-31 37.52591472462699 39.0 35.0 40.5 33.5 41.0 32-33 37.34396360490892 39.0 35.0 41.0 33.0 41.0 34-35 37.23551723928709 39.0 35.0 41.0 33.0 41.0 36-37 37.20916308754169 39.0 35.0 41.0 33.0 41.0 38-39 37.003244229147356 39.0 35.0 41.0 33.0 41.0 40-41 36.536336790977785 39.0 35.0 41.0 31.5 41.0 42-43 36.56522871182365 39.0 35.0 41.0 32.0 41.0 44-45 36.56952999302773 38.0 35.0 40.5 32.0 41.0 46-47 36.658098398435556 38.0 35.0 40.0 32.5 41.0 48-49 36.72973128401883 38.0 35.0 40.5 33.0 41.0 50-51 36.76029847541279 38.0 35.0 41.0 33.0 41.0 52-53 36.56430296689477 38.0 35.0 40.0 32.5 41.0 54-55 36.41246055180386 37.0 35.0 40.0 32.5 41.0 56-57 36.28508782343036 37.0 35.0 40.0 32.0 41.0 58-59 35.88804824188263 36.5 35.0 40.0 31.0 41.0 60-61 35.623607821288296 36.0 35.0 40.0 31.0 41.0 62-63 35.358988807172025 35.0 35.0 39.0 31.0 41.0 64-65 35.12343351453849 35.0 34.0 39.0 31.0 41.0 66-67 34.86844997706247 35.0 34.0 38.5 31.0 40.5 68-69 34.541461066480316 35.0 34.0 37.5 30.0 40.0 70-71 34.24929004458507 35.0 34.0 37.0 30.0 39.5 72-73 33.896098338288844 35.0 34.0 36.5 29.0 39.0 74-75 33.52725338463742 35.0 33.0 36.0 29.0 39.0 76-77 32.395991564687655 34.0 32.0 35.0 27.0 37.0 78-79 32.389015997178376 35.0 32.0 35.0 27.0 36.5 80-81 32.04105375717771 34.0 32.0 35.0 26.5 36.0 82-83 32.09782849271025 34.5 32.5 35.0 27.0 36.0 84-85 31.967114917940627 35.0 33.0 35.0 27.0 36.0 86-87 31.930343250417927 35.0 33.0 35.0 27.0 35.5 88-89 31.765896604111447 35.0 33.0 35.0 27.0 35.0 90-91 31.457447785136956 35.0 33.0 35.0 25.0 35.0 92-93 31.126688823343045 34.0 32.5 35.0 24.0 35.0 94-95 30.91157193691665 34.0 32.0 35.0 23.5 35.0 96-97 30.644263797668575 34.0 32.0 35.0 21.5 35.0 98-99 30.274970051950405 34.0 31.5 35.0 16.5 35.0 100-101 29.255670014569752 33.5 30.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36840320584083486 1101 2 0.2613084123934257 1101 3 0.4265370272150335 1101 4 0.17467441032066944 1101 5 0.19044847097652706 1101 6 0.1808676018383366 1101 7 0.18276868208715769 1101 8 0.1963339701969815 1101 9 0.28540980312394026 1101 10-11 0.3263601406379735 1101 12-13 0.31123260988791657 1101 14-15 0.34458613536880733 1101 16-17 0.3622048781668923 1101 18-19 0.4429853321196262 1101 20-21 0.5072684523016235 1101 22-23 0.4921076914066518 1101 24-25 0.5713581446678049 1101 26-27 0.6252364971663695 1101 28-29 0.577473973308301 1101 30-31 0.6056477725700873 1101 32-33 0.7198672412611415 1101 34-35 0.735006265508396 1101 36-37 0.7088793793121155 1101 38-39 0.743682098074629 1101 40-41 0.8823950980545376 1101 42-43 0.8631142075656726 1101 44-45 0.7024898174991918 1101 46-47 0.7064165691602184 1101 48-49 0.745544503328226 1101 50-51 0.7516709332613729 1101 52-53 0.7674832417331601 1101 54-55 0.7573711403780621 1101 56-57 0.709330882204128 1101 58-59 0.7123035891732812 1101 60-61 0.7092940347783738 1101 62-63 0.6723556501379946 1101 64-65 0.6344100174391869 1101 66-67 0.5898198225626956 1101 68-69 0.5801797142594438 1101 70-71 0.5811803542133092 1101 72-73 0.5689858751864634 1101 74-75 0.5577219150546355 1101 76-77 0.40247788942453155 1101 78-79 0.2690528175046367 1101 80-81 0.25445077449627007 1101 82-83 0.32140112115526165 1101 84-85 0.3693086906604641 1101 86-87 0.5480464945912793 1101 88-89 0.5971954957301229 1101 90-91 0.7056126763746562 1101 92-93 0.7144918232787667 1101 94-95 0.7127068799381213 1101 96-97 0.7035392782657155 1101 98-99 0.8064152349717979 1101 100-101 0.9052998541405692 1102 1 0.3691230956946612 1102 2 0.2952719056251709 1102 3 0.40657785687980663 1102 4 0.17895623613284783 1102 5 0.1580720456819762 1102 6 0.12706703661746843 1102 7 0.131437750547704 1102 8 0.1267231569435907 1102 9 0.178661144661163 1102 10-11 0.22591118609987149 1102 12-13 0.2119146234882905 1102 14-15 0.24459801827796213 1102 16-17 0.2050571088305304 1102 18-19 0.30586762330187156 1102 20-21 0.3198368382498771 1102 22-23 0.3642232357285238 1102 24-25 0.39816669144121164 1102 26-27 0.47559685075010094 1102 28-29 0.4632695110805827 1102 30-31 0.4407572392646273 1102 32-33 0.48566075098737826 1102 34-35 0.5169094343441998 1102 36-37 0.515400605709246 1102 38-39 0.5293971096742496 1102 40-41 0.6404869533024709 1102 42-43 0.6271441871840864 1102 44-45 0.5082235663603214 1102 46-47 0.47933689313226324 1102 48-49 0.49977418591546297 1102 50-51 0.6079712397816976 1102 52-53 0.5910501702310995 1102 54-55 0.54342717678648 1102 56-57 0.530161477883091 1102 58-59 0.6111822681062975 1102 60-61 0.5389110568448743 1102 62-63 0.525211176376466 1102 64-65 0.43948558501156043 1102 66-67 0.5066336818747601 1102 68-69 0.46938091208107124 1102 70-71 0.40912887896912054 1102 72-73 0.3495817023708909 1102 74-75 0.40254495764734344 1102 76-77 0.31186614401800483 1102 78-79 0.07298765073317526 1102 80-81 0.08066904904743666 1102 82-83 0.09751878162682459 1102 84-85 0.26700210966489024 1102 86-87 0.3915545875893862 1102 88-89 0.4369059201223102 1102 90-91 0.5682156762481725 1102 92-93 0.5668621505824056 1102 94-95 0.6022166427495499 1102 96-97 0.6238742355596898 1102 98-99 0.5930707224502036 1102 100-101 0.8117516559777549 1103 1 0.3378897945933801 1103 2 0.34940912004871194 1103 3 0.4407079792356896 1103 4 0.19120023540193642 1103 5 0.16637986613520184 1103 6 0.12309281563565122 1103 7 0.11558900239103309 1103 8 0.13342708967307715 1103 9 0.16754041115126483 1103 10-11 0.2152803242203376 1103 12-13 0.1865763048792175 1103 14-15 0.18926004960295728 1103 16-17 0.18390765057603176 1103 18-19 0.2238597904819386 1103 20-21 0.27356770165426525 1103 22-23 0.3033211879294626 1103 24-25 0.32956151137541667 1103 26-27 0.39866563221240625 1103 28-29 0.34420579307818144 1103 30-31 0.35334265098408224 1103 32-33 0.37028148720701637 1103 34-35 0.2910632294969844 1103 36-37 0.2862059112367632 1103 38-39 0.3035100807886195 1103 40-41 0.3040596905080619 1103 42-43 0.2855443631580812 1103 44-45 0.26699228922152685 1103 46-47 0.29064592262044897 1103 48-49 0.3743310428898994 1103 50-51 0.4299467472917726 1103 52-53 0.3894860668900719 1103 54-55 0.352169160732025 1103 56-57 0.33788266130422784 1103 58-59 0.47260809319921293 1103 60-61 0.45221290050402985 1103 62-63 0.43402358040577127 1103 64-65 0.3587090397173611 1103 66-67 0.42774267626758444 1103 68-69 0.4570207449919508 1103 70-71 0.38980957060406496 1103 72-73 0.44302775560533547 1103 74-75 0.5258063047932993 1103 76-77 0.4019412410617136 1103 78-79 0.18769088103626075 1103 80-81 0.22188508431425902 1103 82-83 0.31827288555351885 1103 84-85 0.5863311283757646 1103 86-87 0.5932670654760805 1103 88-89 0.6292472939678895 1103 90-91 0.7011101427932118 1103 92-93 0.6540370866819281 1103 94-95 0.7179456915267544 1103 96-97 0.7269665072780462 1103 98-99 0.7530748342356226 1103 100-101 0.9887682426554498 1104 1 0.2004525421126786 1104 2 0.24971795793034346 1104 3 0.14084528967855192 1104 4 0.09689801804142206 1104 5 0.13544026561590528 1104 6 0.12012909981272912 1104 7 0.10449142817655144 1104 8 0.11662849430759081 1104 9 0.1654641271904751 1104 10-11 0.17589245660834507 1104 12-13 0.1875437552842456 1104 14-15 0.249072825776814 1104 16-17 0.2466874825284009 1104 18-19 0.2824694648347261 1104 20-21 0.2614623054630485 1104 22-23 0.3094818820739462 1104 24-25 0.34235473688936935 1104 26-27 0.34305644263095303 1104 28-29 0.29816258955925434 1104 30-31 0.32119905421493655 1104 32-33 0.27098354994864593 1104 34-35 0.23014048078590577 1104 36-37 0.24484867447301184 1104 38-39 0.23816350088797833 1104 40-41 0.23062156209400086 1104 42-43 0.1983536267991326 1104 44-45 0.29458035865378207 1104 46-47 0.27080606332410895 1104 48-49 0.3085727177924369 1104 50-51 0.3650393658907305 1104 52-53 0.22751682246016713 1104 54-55 0.33234498224415177 1104 56-57 0.21174031893635714 1104 58-59 0.40166365714880925 1104 60-61 0.3052419995240925 1104 62-63 0.2894221662001897 1104 64-65 0.23954901151245878 1104 66-67 0.2819720692816219 1104 68-69 0.2617031285974747 1104 70-71 0.16608816572445306 1104 72-73 0.25477083250802224 1104 74-75 0.21067988004692495 1104 76-77 0.05935910875716388 1104 78-79 -0.07773643838481092 1104 80-81 0.0131448521162838 1104 82-83 0.13183518531949545 1104 84-85 0.3169577227488496 1104 86-87 0.2831993397359902 1104 88-89 0.25429976467026094 1104 90-91 0.41389398110722553 1104 92-93 0.3824736959483239 1104 94-95 0.4235837322107123 1104 96-97 0.37065137879498167 1104 98-99 0.3751901842191039 1104 100-101 0.6972463347590541 1105 1 0.20952933451966516 1105 2 0.2854890711694651 1105 3 0.3055790737060775 1105 4 0.09979718197268284 1105 5 0.09588535671348097 1105 6 0.043255718934887 1105 7 0.02872145433286022 1105 8 0.016760911686482416 1105 9 0.010958301906882184 1105 10-11 0.05622725607759804 1105 12-13 -0.02593334030719774 1105 14-15 -0.040139446578812965 1105 16-17 -0.01891588709489156 1105 18-19 -0.039039671523454444 1105 20-21 -0.028154960801572315 1105 22-23 -0.017088140105151695 1105 24-25 0.09695668177051431 1105 26-27 0.10583657245660305 1105 28-29 0.08541537915772324 1105 30-31 0.08401549249042972 1105 32-33 0.08681946434690246 1105 34-35 0.005764733635530206 1105 36-37 0.0019274530695554404 1105 38-39 -0.03765043782990318 1105 40-41 -0.09639123825262885 1105 42-43 -0.07689655300059428 1105 44-45 -0.0834848604259335 1105 46-47 -0.07570734398885293 1105 48-49 0.04616486515310925 1105 50-51 0.09434580764273193 1105 52-53 0.05518758454928019 1105 54-55 0.08486977085144787 1105 56-57 0.051570962243850715 1105 58-59 0.21414068431962363 1105 60-61 0.20485062928515418 1105 62-63 0.20442275154503164 1105 64-65 0.09933980727419822 1105 66-67 0.1405855431091041 1105 68-69 0.1954484045872178 1105 70-71 0.05134133768184057 1105 72-73 0.1109786848344001 1105 74-75 0.2430460003727717 1105 76-77 0.19829397886609001 1105 78-79 0.051022250750001774 1105 80-81 0.07385266916551103 1105 82-83 0.21428369133169411 1105 84-85 0.5112800129080561 1105 86-87 0.426666187169662 1105 88-89 0.33231494138892614 1105 90-91 0.4535929147127611 1105 92-93 0.3384910231513949 1105 94-95 0.26215085213201306 1105 96-97 0.2862043433283503 1105 98-99 0.3475343473566319 1105 100-101 0.5860809565622738 1106 1 0.15533868505385584 1106 2 0.18859727977617524 1106 3 0.24114479642386755 1106 4 0.06603646163036814 1106 5 0.013786260274642359 1106 6 -0.02902728830598278 1106 7 -0.021828560092068017 1106 8 -0.0242702911428907 1106 9 -0.061366780192869896 1106 10-11 -0.05182371892917814 1106 12-13 -0.04985731952472605 1106 14-15 -0.12009100827006591 1106 16-17 -0.11385398677501257 1106 18-19 -0.2189592675954728 1106 20-21 -0.22595412367385137 1106 22-23 -0.1887402596517589 1106 24-25 -0.2405771174158673 1106 26-27 -0.2883490869853702 1106 28-29 -0.2580866001099835 1106 30-31 -0.21683101881310307 1106 32-33 -0.20165076025898543 1106 34-35 -0.27497389550103435 1106 36-37 -0.20719244493590594 1106 38-39 -0.2913471716027942 1106 40-41 -0.33811622336899205 1106 42-43 -0.2988628759829197 1106 44-45 -0.2935039514234177 1106 46-47 -0.3439435104438857 1106 48-49 -0.3669827196644704 1106 50-51 -0.2637555483469143 1106 52-53 -0.3023453137919532 1106 54-55 -0.3022433341699937 1106 56-57 -0.2998769422389884 1106 58-59 -0.2813437581158027 1106 60-61 -0.2601305382308894 1106 62-63 -0.2411375545691996 1106 64-65 -0.39438578749352615 1106 66-67 -0.21225097101618218 1106 68-69 -0.20073664644736766 1106 70-71 -0.3610921407611656 1106 72-73 -0.43288283621826196 1106 74-75 -0.20367960245486216 1106 76-77 -0.211368506645492 1106 78-79 -0.3481180213612163 1106 80-81 -0.3497395523165494 1106 82-83 -0.1432515200253448 1106 84-85 0.10060060384381231 1106 86-87 -0.023761018426316838 1106 88-89 -0.036532653624060885 1106 90-91 0.030573793108306546 1106 92-93 -0.24767336701531306 1106 94-95 -0.3009022761175508 1106 96-97 -0.21271555927982178 1106 98-99 -0.30373146317541355 1106 100-101 0.03039668848029109 1107 1 -0.0016732628838305175 1107 2 0.06847967827488333 1107 3 0.028176546738897912 1107 4 -3.580741295436951E-4 1107 5 -0.052445281064791516 1107 6 -0.08509147226853031 1107 7 -0.10898826841273745 1107 8 -0.14917809369056556 1107 9 -0.23225518105512322 1107 10-11 -0.22041697744888467 1107 12-13 -0.1814619582528323 1107 14-15 -0.2616061809102703 1107 16-17 -0.15815902692830264 1107 18-19 -0.3163620191522156 1107 20-21 -0.3709701218672379 1107 22-23 -0.40878526075346855 1107 24-25 -0.37548857084554044 1107 26-27 -0.3839230372602813 1107 28-29 -0.33084696043658113 1107 30-31 -0.37599596491772047 1107 32-33 -0.4117278701492779 1107 34-35 -0.4412817423809301 1107 36-37 -0.368108935191529 1107 38-39 -0.575280462542004 1107 40-41 -0.486132906060071 1107 42-43 -0.5263460768823762 1107 44-45 -0.4348222119501628 1107 46-47 -0.46494330446018495 1107 48-49 -0.543888134561918 1107 50-51 -0.4422046464022813 1107 52-53 -0.5044043716897306 1107 54-55 -0.501739612733779 1107 56-57 -0.641655406451271 1107 58-59 -0.46812608347799767 1107 60-61 -0.4002138381075113 1107 62-63 -0.26863863324422965 1107 64-65 -0.6173743099957392 1107 66-67 -0.3792998607856646 1107 68-69 -0.33766132548807093 1107 70-71 -0.3632693465155086 1107 72-73 -0.35068325873378825 1107 74-75 -0.22391629516419442 1107 76-77 -0.33871060617581605 1107 78-79 -0.44304152108899686 1107 80-81 -0.3908085526680054 1107 82-83 -0.2719377394470577 1107 84-85 -0.06325497353077125 1107 86-87 -0.19484227963979706 1107 88-89 -0.2331886086122985 1107 90-91 -0.22271574349026224 1107 92-93 -0.4912477635078325 1107 94-95 -0.4266705587956956 1107 96-97 -0.40653270899991867 1107 98-99 -0.37554965195919365 1107 100-101 -0.0985643298713228 1108 1 -0.2212579057495745 1108 2 -0.03972266775496536 1108 3 -0.21032312050494184 1108 4 -0.06121803257084224 1108 5 -0.13119745402275385 1108 6 -0.2191868824837897 1108 7 -0.180070457954443 1108 8 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20-21 -0.01653575032073462 2305 22-23 0.014989655416123071 2305 24-25 0.052501326609096566 2305 26-27 0.0708016338736499 2305 28-29 0.004124657319522385 2305 30-31 -0.012023648795882025 2305 32-33 6.325023404940566E-4 2305 34-35 0.020019198656299864 2305 36-37 0.028838733280807105 2305 38-39 0.01845049378042063 2305 40-41 -0.07314434822242788 2305 42-43 -0.045280689239973526 2305 44-45 -0.11738360155801075 2305 46-47 -0.015889386137580175 2305 48-49 0.06833931543705063 2305 50-51 -0.18220894076265637 2305 52-53 -0.02031428925610612 2305 54-55 0.14227116788342897 2305 56-57 0.0900292581215254 2305 58-59 0.12429971872564494 2305 60-61 0.15953433737956146 2305 62-63 0.06845505212499603 2305 64-65 0.08508668452089552 2305 66-67 0.019286366264211097 2305 68-69 0.052445178287570116 2305 70-71 0.066062112811899 2305 72-73 0.02075558847647585 2305 74-75 -0.04049285328478902 2305 76-77 0.22921592230574817 2305 78-79 0.19313021660885 2305 80-81 0.3284658187334841 2305 82-83 0.1986500784487859 2305 84-85 0.19599333781451023 2305 86-87 0.1453920583140338 2305 88-89 0.203519871882488 2305 90-91 0.19230118553478093 2305 92-93 0.18919230676816667 2305 94-95 0.19333503628394766 2305 96-97 0.2843510874221167 2305 98-99 0.28429661159055186 2305 100-101 0.32714950884627214 2306 1 -0.6716406255415706 2306 2 -0.13193729148161992 2306 3 -0.18201415574934998 2306 4 -0.194437854306301 2306 5 -0.17094494066043353 2306 6 -0.1768189271724765 2306 7 -0.19383768312218308 2306 8 -0.14337483088481662 2306 9 -0.20386226619359604 2306 10-11 -0.1114825626525473 2306 12-13 -0.11980657023875096 2306 14-15 -0.14851202061563384 2306 16-17 -0.1566952072643346 2306 18-19 -0.17819348559877568 2306 20-21 -0.16199662264637738 2306 22-23 -0.19699175020951287 2306 24-25 -0.15032943016777978 2306 26-27 -0.15977993127540202 2306 28-29 -0.22037988766695094 2306 30-31 -0.21762116260376274 2306 32-33 -0.24269666146383884 2306 34-35 -0.20450049821347704 2306 36-37 -0.5130832407787125 2306 38-39 -0.5496575634978029 2306 40-41 -0.36050669933189994 2306 42-43 -0.2805679949279849 2306 44-45 -0.4338693134004359 2306 46-47 -0.4511931217052947 2306 48-49 -0.2792637260471267 2306 50-51 -0.30499851449984305 2306 52-53 -0.3266939789375414 2306 54-55 -0.17953971305158944 2306 56-57 -0.3959610244776641 2306 58-59 -0.4768604337882394 2306 60-61 -0.2805476426712801 2306 62-63 -0.40311922592011484 2306 64-65 -0.36519350172788023 2306 66-67 -0.38905801411211627 2306 68-69 -0.3656333062590349 2306 70-71 -0.20116335909747107 2306 72-73 -0.22880097306735792 2306 74-75 -0.3443303661922883 2306 76-77 -0.042420167639534156 2306 78-79 -0.06481861599139904 2306 80-81 -0.1216355426850626 2306 82-83 -0.14016701649393326 2306 84-85 -0.04316333602471545 2306 86-87 -0.2481352790809126 2306 88-89 -0.11789605557107663 2306 90-91 -0.1890080539374459 2306 92-93 -0.2921936282660731 2306 94-95 -0.18976339815467824 2306 96-97 -0.16081508314002235 2306 98-99 -0.19826006807591767 2306 100-101 -0.22199760473836605 2307 1 -1.068577502763432 2307 2 -1.179952879381954 2307 3 -0.9657136728409235 2307 4 -0.5403105836061783 2307 5 -0.6963130583898405 2307 6 -0.5023613527489061 2307 7 -0.3491213387191152 2307 8 -0.5074733044296664 2307 9 -0.5268257481802081 2307 10-11 -0.4528136134361276 2307 12-13 -0.3036326766418611 2307 14-15 -0.359360685192307 2307 16-17 -0.68538890203601 2307 18-19 -0.4877074596303217 2307 20-21 -0.4530028914956361 2307 22-23 -0.40920560363917247 2307 24-25 -0.4460501780886901 2307 26-27 -0.5354268469485035 2307 28-29 -0.5804154680985363 2307 30-31 -0.5887867980974093 2307 32-33 -0.527442136945254 2307 34-35 -0.5935566191811859 2307 36-37 -0.530300857335952 2307 38-39 -0.5176205891228705 2307 40-41 -0.5493280445484459 2307 42-43 -0.652818569693089 2307 44-45 -0.5382168202843545 2307 46-47 -1.0089392652183875 2307 48-49 -0.7527911252705408 2307 50-51 -0.7062411072047681 2307 52-53 -0.6878162842014888 2307 54-55 -0.4633876867785247 2307 56-57 -0.48516035670571256 2307 58-59 -0.7146984033652473 2307 60-61 -0.5116676318076472 2307 62-63 -0.6629285595524905 2307 64-65 -0.5465292051730231 2307 66-67 -0.6397008577787915 2307 68-69 -0.6243550292353319 2307 70-71 -0.66903710667723 2307 72-73 -0.7476117598216732 2307 74-75 -0.7421832222456644 2307 76-77 -0.55382410919335 2307 78-79 -0.5199650570299497 2307 80-81 -0.45796522809427387 2307 82-83 -0.5731905573289211 2307 84-85 -0.49114507711077593 2307 86-87 -0.6280086823488702 2307 88-89 -0.5200340444599725 2307 90-91 -0.5943739056277373 2307 92-93 -0.6408026716821915 2307 94-95 -0.6888766236790893 2307 96-97 -0.71840528921323 2307 98-99 -0.8240150032271281 2307 100-101 -0.9466519155090722 2308 1 -2.0516006497085506 2308 2 -3.1509744102656896 2308 3 -2.7685478965944483 2308 4 -1.1759395549059448 2308 5 -0.8157003115481558 2308 6 -0.5502044889958242 2308 7 -0.5048083259370486 2308 8 -0.4990582711673568 2308 9 -0.48239776873838736 2308 10-11 -0.4957784537091072 2308 12-13 -0.5037130402633991 2308 14-15 -0.5991002486865895 2308 16-17 -1.0293610182334518 2308 18-19 -0.972107397068477 2308 20-21 -0.7770607242830252 2308 22-23 -0.8784324750320067 2308 24-25 -1.0771974409911351 2308 26-27 -1.2692761207020382 2308 28-29 -1.2382802356664513 2308 30-31 -0.856775693285968 2308 32-33 -0.9244138501223276 2308 34-35 -0.8804802581804125 2308 36-37 -0.9506628424271284 2308 38-39 -1.1807900687788617 2308 40-41 -1.503554761443688 2308 42-43 -0.9016689860102005 2308 44-45 -0.9156807044687554 2308 46-47 -0.732547545946538 2308 48-49 -0.6357428730473274 2308 50-51 -1.1525511215727775 2308 52-53 -1.5754553617441829 2308 54-55 -0.9949924974742004 2308 56-57 -0.9188610115156948 2308 58-59 -1.2736896117613838 2308 60-61 -1.0504728731950692 2308 62-63 -1.2319382112021557 2308 64-65 -1.5197637413046508 2308 66-67 -0.8818673946287845 2308 68-69 -1.1953071840676799 2308 70-71 -0.7248687149717199 2308 72-73 -0.7173559701281036 2308 74-75 -1.0618465313673724 2308 76-77 -0.9721773837711645 2308 78-79 -0.7440592658466834 2308 80-81 -0.8231028992666864 2308 82-83 -0.8002173114473052 2308 84-85 -0.44282655675216986 2308 86-87 -0.945251699651994 2308 88-89 -0.673957244066937 2308 90-91 -1.4893581067980826 2308 92-93 -1.3755166966418209 2308 94-95 -0.9046118633413371 2308 96-97 -0.8440402356174914 2308 98-99 -0.9709113608262037 2308 100-101 -0.9240137414594365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 4.0 11 42.0 12 201.0 13 765.0 14 2206.0 15 4041.0 16 6527.0 17 9808.0 18 15715.0 19 25449.0 20 27539.0 21 30204.0 22 33885.0 23 39100.0 24 46235.0 25 56554.0 26 68629.0 27 88406.0 28 111754.0 29 130454.0 30 160511.0 31 217642.0 32 282745.0 33 370505.0 34 599370.0 35 1245595.0 36 979970.0 37 1673123.0 38 1218269.0 39 136182.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.600139956793882 40.79790676988216 17.978418577945035 15.623534695378918 2 20.63183185306415 36.779866773794964 27.09395838854306 15.494342984597823 3 19.5572569972655 33.974275858057275 23.52472530045376 22.943741844223464 4 24.23557976909903 33.78339342752135 24.436438920773806 17.544587882605818 5 19.97960287870598 38.243679121520366 21.879711327445815 19.897006672327844 6 21.369136981758928 33.746762827539065 21.184476028502157 23.69962416219985 7 23.57510991943243 34.95451141217881 22.188279780337883 19.282098888050882 8 23.998629815726837 33.543978720726955 22.142510244066383 20.31488121947983 9 21.302421617360874 33.12072262579434 22.508033410970075 23.068822345874715 10-11 22.270038417392318 33.98469846581042 22.355213282082005 21.390049834715256 12-13 21.89090969525403 33.61887852237471 23.024431171718597 21.46578061065267 14-15 20.841864009870392 34.26907633382585 23.132945861893127 21.75611379441063 16-17 21.162679440983812 36.33894755391506 23.018409905635615 19.47996309946551 18-19 19.021746729060965 34.72117251263674 26.191828172455388 20.0652525858469 20-21 18.194201857108826 35.709675785488336 25.36822053582805 20.727901821574786 22-23 17.88581147013597 35.63011329477085 27.760015152750224 18.72406008234296 24-25 17.965538836704585 33.6794868852472 30.360793208486704 17.994181069561517 26-27 17.821615408743064 33.63983754056935 30.611655113486023 17.926891937201557 28-29 18.039587878960546 33.815192900788794 30.561387763995917 17.583831456254746 30-31 18.019031157842644 33.758449227866606 30.51164142219535 17.710878192095404 32-33 17.958930612591608 33.78799498038769 30.532613630458084 17.72046077656262 34-35 18.149533691522823 34.12533097178354 29.932419367135253 17.79271596955838 36-37 18.256396640198812 34.2372554734012 29.553355847465014 17.95299203893498 38-39 18.131766669805902 34.680006310128576 29.11431198890135 18.073915031164173 40-41 18.51996356375591 35.75799759993521 27.324505484562213 18.397533351746667 42-43 18.641305595147745 36.44291826563267 26.614134582485544 18.301641556734044 44-45 18.31396108032989 37.45224206096397 26.18276659567772 18.051030263028423 46-47 18.227315523048194 38.29315506692509 25.712804469639234 17.766724940387487 48-49 18.1753466547745 38.31533416641803 25.77436251741419 17.734956661393277 50-51 18.012079991959336 38.36451333529074 25.84425009318783 17.779156579562095 52-53 18.14280675679704 38.52304476366168 25.69245878961076 17.641689689930516 54-55 18.085687166934296 38.49302416868937 25.723936887106998 17.697351777269343 56-57 17.914935140478182 38.575119152480084 25.878953162332014 17.630992544709713 58-59 17.9018901631374 38.71186058836673 25.70070258216687 17.685546666329003 60-61 18.006243386829613 38.558466691516756 25.5751529190544 17.860137002599235 62-63 18.054459680731764 38.718145655811504 25.548159444708684 17.679235218748047 64-65 17.969350766382526 38.87415118614325 25.46200191994631 17.69449612752791 66-67 17.917197237155577 38.690908165206125 25.50920257860555 17.88269201903274 68-69 17.977772624857842 38.875615283701215 25.322727990986436 17.823884100454503 70-71 18.0598280384443 39.06601391657704 25.166979666982435 17.70717837799622 72-73 18.092981274587935 38.93957392386851 25.181989964468592 17.78545483707496 74-75 17.879414287112432 39.11363006238111 25.107070377718703 17.899885272787756 76-77 17.91311491108179 39.33437904193149 24.77266917647282 17.979836870513893 78-79 17.949512903975513 39.28547686448867 24.657546262185313 18.1074639693505 80-81 17.697299016996986 39.37297318116216 24.74766080737462 18.18206699446624 82-83 17.887803170417527 39.473837631844624 24.55804698355014 18.080312214187714 84-85 17.956859771901517 39.14154024645906 24.48972243084513 18.41187755079429 86-87 17.878174420711996 39.284546964688346 24.62770373313221 18.209574881467454 88-89 17.923350403919454 39.364010529895154 24.46404136827435 18.248597697911038 90-91 18.09964225897327 39.18062241820905 24.279861852502854 18.43987347031483 92-93 18.0500278178536 39.20213541926346 24.228057860080284 18.519778902802653 94-95 18.029081989727047 39.43186024014893 24.0924705551515 18.44658721497252 96-97 17.893924542121596 39.31955599853669 23.978120841212476 18.808398618129235 98-99 18.126437882297637 39.118523577642414 24.033682421675397 18.721356118384556 100-101 17.989709636479084 39.218214112264896 23.629492570166548 19.16258368108948 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 984618.0 1 553366.5 2 80669.5 3 32476.0 4 22609.5 5 17965.5 6 15710.0 7 14707.0 8 14085.5 9 13781.5 10 14522.5 11 16444.0 12 25286.0 13 43298.5 14 59765.5 15 56953.0 16 138622.0 17 154500.0 18 73347.5 19 57274.0 20 38604.5 21 24737.0 22 18335.0 23 17008.0 24 17949.5 25 19467.5 26 22254.0 27 25503.0 28 28684.0 29 33660.0 30 39889.5 31 46989.0 32 54070.5 33 62008.5 34 70896.5 35 80152.5 36 92671.5 37 105949.5 38 117578.0 39 128321.0 40 136397.0 41 141549.5 42 148312.5 43 156890.0 44 165688.5 45 179385.5 46 197312.5 47 215838.0 48 232185.0 49 246804.5 50 248598.0 51 241013.5 52 241821.0 53 247052.0 54 243727.5 55 230729.5 56 214476.5 57 197298.0 58 176902.5 59 156423.5 60 137570.5 61 117096.0 62 97615.5 63 76240.0 64 54589.0 65 36501.0 66 24415.0 67 18159.0 68 13631.5 69 10381.0 70 8245.5 71 6111.5 72 4947.5 73 3953.0 74 3157.0 75 2877.0 76 2315.5 77 1369.0 78 721.5 79 366.0 80 166.5 81 98.0 82 63.5 83 29.0 84 8.5 85 3.5 86 2.0 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026116334817743597 2 0.006872025474769906 3 0.0023610221880687393 4 3.9570204269308485E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.978510213465424E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 6.595034044884747E-6 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7581462.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.53181607702525 #Duplication Level Percentage of deduplicated Percentage of total 1 77.4664711545555 27.52524405199848 2 11.480301392857903 8.158319151996876 3 3.3276076038252516 3.547078240668886 4 1.505062288662292 2.13910385700861 5 0.9071935030186086 1.6117116347764724 6 0.6074116379905041 1.2949463162473942 7 0.4648406968101663 1.156164390092256 8 0.3675079985997171 1.044658129046464 9 0.3182890727703547 1.0178449913702832 >10 3.3076308600395654 24.328073462887293 >50 0.21695756240866018 5.050824278955849 >100 0.025450207098788747 1.5403112507696815 >500 0.0022405242826447165 0.5550843294221504 >1k 0.0020959384603249235 1.4825291908577845 >5k 6.504636598125728E-4 1.7011830180801375 >10k+ 2.890949599166991E-4 17.846923705821347 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 995223 13.127059134504664 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 176765 2.3315423858881044 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 101051 1.3328695705392972 No Hit GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29522 0.38939719014617497 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 27898 0.36797651956838934 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25672 0.33861542800056244 No Hit TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21233 0.2800647157500757 No Hit AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12574 0.1658519161607616 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTAAAAAAAAAA 9921 0.13085866551860315 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 9672 0.12757433856425054 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 9132 0.12045170179577501 No Hit GGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9013 0.11888208369309244 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTAAAAAAAAA 8478 0.11182539726506575 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8163 0.10767052581678836 No Hit AATTCGAGGCGGTACAGGTGTTCTCCCGTATTGTTGACATGCCAGCGGGT 7632 0.10066659966112078 No Hit GGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7599 0.10023132741415837 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.2975170224423735E-4 0.0 0.0 0.0 0.0 2 3.2975170224423735E-4 0.0 0.0 0.0 0.0 3 3.429417703340068E-4 0.0 0.0 0.0 0.0 4 3.429417703340068E-4 0.0 0.0 0.0 0.0 5 3.429417703340068E-4 0.0 0.0 0.0 0.0 6 3.429417703340068E-4 0.0 0.0 0.0 0.0 7 3.429417703340068E-4 0.0 0.0 0.0 1.3190068089769494E-5 8 3.561318384237763E-4 0.0 0.0 0.0 1.3190068089769494E-5 9 3.693219065135458E-4 0.0 0.0 0.0 1.3190068089769494E-5 10-11 3.825119746033153E-4 0.0 0.0 0.0 1.3190068089769494E-5 12-13 4.220821788726238E-4 0.0 0.0 0.0 2.6380136179538988E-5 14-15 4.8803251932147125E-4 0.0 0.0 0.0 3.957020426930848E-5 16-17 4.8803251932147125E-4 0.0 0.0 0.0 3.957020426930848E-5 18-19 5.144126555010101E-4 0.0 0.0 6.595034044884747E-6 5.2760272359077975E-5 20-21 5.605778938152035E-4 0.0 0.0 1.3190068089769494E-5 5.2760272359077975E-5 22-23 6.595034044884747E-4 0.0 0.0 1.3190068089769494E-5 5.935530640396272E-5 24-25 7.979991194310543E-4 0.0 0.0 1.3190068089769494E-5 6.595034044884746E-5 26-27 0.0010815855833610985 0.0 0.0 1.3190068089769494E-5 7.914040853861696E-5 28-29 0.0014904776941439529 0.0 0.0 1.3190068089769494E-5 7.914040853861696E-5 30-31 0.002189551302901736 0.0 0.0 1.3190068089769494E-5 7.914040853861696E-5 32-33 0.0030271206266020987 1.3190068089769494E-5 0.0 1.3190068089769494E-5 9.89255106732712E-5 34-35 0.0039702104950206174 1.3190068089769494E-5 0.0 1.3190068089769494E-5 1.0552054471815595E-4 36-37 0.0052826222699526824 1.3190068089769494E-5 0.0 1.3190068089769494E-5 1.121155787630407E-4 38-39 0.006693959555558017 1.3190068089769494E-5 0.0 1.3190068089769494E-5 1.2530564685281017E-4 40-41 0.007861280581502619 1.3190068089769494E-5 0.0 1.3190068089769494E-5 1.5168578303234917E-4 42-43 0.009002221471267679 1.3190068089769494E-5 0.0 1.3190068089769494E-5 1.6487585112211868E-4 44-45 0.010710335288892828 1.3190068089769494E-5 0.0 1.3190068089769494E-5 1.714708851670034E-4 46-47 0.012359093800114015 1.3190068089769494E-5 0.0 1.3190068089769494E-5 1.714708851670034E-4 48-49 0.014172728162457321 1.3190068089769494E-5 0.0 1.3190068089769494E-5 1.846609532567729E-4 50-51 0.016322709261089748 1.3190068089769494E-5 0.0 1.3190068089769494E-5 2.110410894363119E-4 52-53 0.020418225402963175 1.3190068089769494E-5 0.0 1.3190068089769494E-5 2.2423115752608137E-4 54-55 0.026004219238980556 1.3190068089769494E-5 0.0 1.3190068089769494E-5 2.3082619157096612E-4 56-57 0.031247271304663926 1.3190068089769494E-5 0.0 1.3190068089769494E-5 2.3742122561585087E-4 58-59 0.036061646157429794 1.3190068089769494E-5 0.0 1.3190068089769494E-5 2.3742122561585087E-4 60-61 0.04175316053816533 1.3190068089769494E-5 0.0 1.3190068089769494E-5 2.3742122561585087E-4 62-63 0.047306179203958285 1.3190068089769494E-5 0.0 1.3190068089769494E-5 2.3742122561585087E-4 64-65 0.05365060195513741 1.3190068089769494E-5 0.0 1.3190068089769494E-5 2.3742122561585087E-4 66-67 0.06209884056663477 1.3190068089769494E-5 0.0 1.3190068089769494E-5 2.3742122561585087E-4 68-69 0.07025689768015721 1.3190068089769494E-5 0.0 1.3190068089769494E-5 2.3742122561585087E-4 70-71 0.11100761304350004 1.3190068089769494E-5 0.0 1.3190068089769494E-5 2.3742122561585087E-4 72-73 0.15003042948708312 1.3190068089769494E-5 0.0 1.3190068089769494E-5 2.3742122561585087E-4 74-75 0.161881705665741 1.3190068089769494E-5 0.0 1.3190068089769494E-5 2.3742122561585087E-4 76-77 0.17532898008326098 1.3190068089769494E-5 0.0 1.978510213465424E-5 2.3742122561585087E-4 78-79 0.19001612090121933 1.3190068089769494E-5 0.0 2.6380136179538988E-5 2.4401625966073562E-4 80-81 0.20705109383915662 1.3190068089769494E-5 0.0 2.6380136179538988E-5 2.5061129370562035E-4 82-83 0.22716594767605508 1.3190068089769494E-5 0.0 2.6380136179538988E-5 2.6380136179538985E-4 84-85 0.25097402057808904 1.3190068089769494E-5 0.0 2.6380136179538988E-5 2.7699142988515935E-4 86-87 0.27877208907727824 1.3190068089769494E-5 0.0 2.6380136179538988E-5 2.7699142988515935E-4 88-89 0.31738601341007844 1.3190068089769494E-5 0.0 2.6380136179538988E-5 2.967765320198136E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 28325 0.0 94.28401 1 TCGACGG 28560 0.0 93.692986 2 CGACGGC 31020 0.0 86.62575 3 GACGGCG 32740 0.0 82.26238 4 ACGGCGC 32785 0.0 82.19186 5 CGGCGCG 34400 0.0 78.30552 6 ATCTCGA 16060 0.0 64.53299 1 ATATCTC 4635 0.0 61.10379 1 TATCTCG 4695 0.0 60.808517 2 TCTCGAG 18445 0.0 56.118248 2 CTCGAGG 21270 0.0 48.77393 3 TCGAGGG 21360 0.0 48.634495 4 CGAGGGC 23125 0.0 44.92191 5 CGAGGCG 1930 0.0 44.05416 5 ATTCCCC 4015 0.0 43.77657 2 GGCGCGC 68345 0.0 43.01351 7 AATTCGA 3650 0.0 42.962814 1 CGCGCCG 68795 0.0 42.68382 9 GCGCGCC 69685 0.0 42.200024 8 GCGGTAC 2165 0.0 39.491695 9 >>END_MODULE