FastQCFastQC Report
Tue 31 May 2016
SRR1051260_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051260_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14515202
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT213685514.721496814167656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1778051.2249571173725313No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT1165200.8027445983872632No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT643210.4431285213943285No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT586290.40391446154176847No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276430.19044171758684447No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT232070.15988065477834892No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199910.13772457317507536No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189920.1308421336471928No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173000.11918538922158989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1658700.094.6113361
ATATCTC2576300.093.913681
TATCTCG2591150.093.232492
TCGACGG1693050.092.7032
CGACGGC1723600.090.935893
ACGGCGC1730750.090.436725
GACGGCG1735000.090.316484
ATCTCGA2690800.089.712673
CGGCGCG1747400.089.561416
TCTCGAG2685400.089.139614
TCGAGGG2728150.087.676656
CTCGAGG2731300.087.5964055
CGAGGGC2756900.086.76067
AGGGCGC2794500.085.792099
GAGGGCG2809000.085.361078
GGGCGCG2807850.042.7682110-11
GGATCCT2804900.041.77857218-19
CCATATT1798700.041.7686718-19
TCCATAT1803450.041.6902618-19
ATCCATA1794300.041.62227616-17
Produced by FastQC (version 0.11.5)