##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051260_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14515202 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07345113075243 33.0 31.0 34.0 30.0 34.0 2 32.35197519125121 34.0 31.0 34.0 30.0 34.0 3 32.40044168865166 34.0 31.0 34.0 30.0 34.0 4 35.91484513959916 37.0 35.0 37.0 35.0 37.0 5 35.858385298392676 37.0 35.0 37.0 35.0 37.0 6 35.849424555028584 37.0 35.0 37.0 35.0 37.0 7 35.826589323386614 37.0 35.0 37.0 35.0 37.0 8 35.87479698870192 37.0 35.0 37.0 35.0 37.0 9 37.45497761588161 39.0 37.0 39.0 35.0 39.0 10-11 37.457743164717925 39.0 37.5 39.0 34.5 39.0 12-13 37.45704506902487 39.0 37.0 39.0 35.0 39.0 14-15 38.81859287938259 40.5 38.0 41.0 35.0 41.0 16-17 38.6771250582665 40.5 38.5 41.0 34.0 41.0 18-19 38.57380265875735 40.0 38.0 41.0 34.0 41.0 20-21 38.560002299657974 40.0 38.0 41.0 34.0 41.0 22-23 38.54856604820243 40.0 38.0 41.0 34.0 41.0 24-25 38.56141574881286 40.0 38.0 41.0 34.0 41.0 26-27 38.62520194345211 40.0 38.0 41.0 34.0 41.0 28-29 38.3903785837772 40.0 38.0 41.0 34.0 41.0 30-31 37.71644207913882 39.5 36.0 40.5 34.0 41.0 32-33 37.008255276089166 38.0 35.0 40.0 33.0 41.0 34-35 36.66781023784581 37.5 35.0 40.0 33.0 41.0 36-37 36.6237886320838 38.0 35.0 40.0 33.0 41.0 38-39 36.357669428231176 38.0 35.0 40.0 32.0 41.0 40-41 35.21881118154608 37.5 35.0 40.0 24.5 41.0 42-43 35.1956310356549 37.0 35.0 40.0 25.0 41.0 44-45 35.21026624362513 37.0 35.0 40.0 25.5 41.0 46-47 35.38478672222405 37.0 35.0 40.0 27.0 41.0 48-49 35.13393068866696 37.0 33.5 40.0 24.0 41.0 50-51 35.173770712939444 37.0 33.5 40.0 26.5 41.0 52-53 35.21549352189518 36.5 34.0 40.0 27.5 41.0 54-55 34.982094530961405 36.0 34.0 40.0 27.5 41.0 56-57 34.634654205983495 35.5 34.0 40.0 26.5 41.0 58-59 34.266247448709294 35.0 34.0 40.0 26.0 41.0 60-61 33.938857240843085 35.0 34.0 39.5 25.5 41.0 62-63 33.57137516928803 35.0 34.0 39.0 25.0 41.0 64-65 33.1825692470556 35.0 33.0 39.0 23.5 41.0 66-67 32.72939539525527 35.0 33.0 38.0 22.0 40.0 68-69 32.32198862957608 35.0 33.0 37.0 20.0 40.0 70-71 31.893157187891703 35.0 33.0 37.0 18.5 39.0 72-73 31.51696225102482 35.0 32.0 36.0 17.5 39.0 74-75 31.153944016762566 35.0 32.0 36.0 13.0 38.5 76-77 30.782609845870557 35.0 32.0 35.0 8.5 37.0 78-79 30.491293610657294 35.0 31.5 35.0 6.0 37.0 80-81 30.21104308434702 35.0 31.0 35.0 2.0 36.5 82-83 29.95413608436176 35.0 31.0 35.0 2.0 36.0 84-85 29.669429712380165 35.0 31.0 35.0 2.0 36.0 86-87 29.403048093991387 35.0 31.0 35.0 2.0 35.0 88-89 29.136653041411343 35.0 30.5 35.0 2.0 35.0 90-91 28.903674058411312 35.0 30.0 35.0 2.0 35.0 92-93 28.658619184218036 34.0 30.0 35.0 2.0 35.0 94-95 28.38688848422502 34.0 29.5 35.0 2.0 35.0 96-97 28.08140010728063 34.0 29.0 35.0 2.0 35.0 98-99 27.755798024719187 34.0 29.0 35.0 2.0 35.0 100-101 26.505126108475793 33.0 26.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2809328320764095 1101 2 0.3016528708710169 1101 3 0.3353835491972035 1101 4 0.2580156735515473 1101 5 0.2735508773653095 1101 6 0.295438164310724 1101 7 0.2652281061536357 1101 8 0.22204933384686854 1101 9 0.30685294813910957 1101 10-11 0.24844820791004452 1101 12-13 0.2962202679252499 1101 14-15 0.40748858246477937 1101 16-17 0.2897231269735272 1101 18-19 0.3706712384743369 1101 20-21 0.3570876952548261 1101 22-23 0.384293761013879 1101 24-25 0.41900408842185044 1101 26-27 0.4188686219755695 1101 28-29 0.4575209846926427 1101 30-31 0.5174452147643862 1101 32-33 0.4229933183964363 1101 34-35 0.4570707864290071 1101 36-37 0.482188886289002 1101 38-39 0.4664281753528101 1101 40-41 0.3826928429076446 1101 42-43 0.3924062754054276 1101 44-45 0.40701584551516135 1101 46-47 0.42874625154501445 1101 48-49 0.5467203199317794 1101 50-51 0.45302604518108325 1101 52-53 0.3128053008014433 1101 54-55 0.2708186914799242 1101 56-57 0.2257891867391706 1101 58-59 0.1842854928556008 1101 60-61 0.14911712459208104 1101 62-63 0.14367221251934126 1101 64-65 0.09746916430910346 1101 66-67 0.08316568811364533 1101 68-69 0.025271311826749354 1101 70-71 0.0670089593268024 1101 72-73 0.048623936989212524 1101 74-75 0.0014579678945949581 1101 76-77 -0.06933012721181342 1101 78-79 -0.18734892134229497 1101 80-81 -0.19182551657107894 1101 82-83 -0.177767214411638 1101 84-85 -0.19455518322741838 1101 86-87 -0.23162445424001277 1101 88-89 -0.22029649412587915 1101 90-91 -0.1986714927619495 1101 92-93 -0.19628480007839855 1101 94-95 -0.17163185628679045 1101 96-97 -0.2214070633920464 1101 98-99 -0.20897664797439575 1101 100-101 -0.021101164705569886 1102 1 0.27255482697353983 1102 2 0.30204612555655075 1102 3 0.3072360470651532 1102 4 0.21783252929787267 1102 5 0.22933178504172247 1102 6 0.23752562705665525 1102 7 0.2407441982156726 1102 8 0.20483217681128707 1102 9 0.24101896968949887 1102 10-11 0.22418803878011317 1102 12-13 0.25566580321513754 1102 14-15 0.3584053691300255 1102 16-17 0.2771620406173554 1102 18-19 0.378001835196649 1102 20-21 0.31974845019269793 1102 22-23 0.3433481669524525 1102 24-25 0.3621631595007031 1102 26-27 0.37748272807819916 1102 28-29 0.4343904880265441 1102 30-31 0.390526974280057 1102 32-33 0.33267316316582907 1102 34-35 0.34259146419015707 1102 36-37 0.42939815024665506 1102 38-39 0.43062875950165136 1102 40-41 0.2767642058623494 1102 42-43 0.23700948371138253 1102 44-45 0.2149135179087125 1102 46-47 0.1987337563892595 1102 48-49 0.3459613991927526 1102 50-51 0.27496016571034687 1102 52-53 0.23491742341426658 1102 54-55 0.18075023041678406 1102 56-57 0.1196885582894609 1102 58-59 0.12120840982395009 1102 60-61 0.08113851388704774 1102 62-63 0.06644771706736918 1102 64-65 -0.03395962658542828 1102 66-67 -0.061859018658786624 1102 68-69 -0.080228797004402 1102 70-71 -0.025337403756509502 1102 72-73 -0.030202731330845722 1102 74-75 -0.04659616562994984 1102 76-77 -0.11438824611004961 1102 78-79 -0.18149227236981957 1102 80-81 -0.13840422935268393 1102 82-83 -0.13680891826099284 1102 84-85 -0.23206597902832726 1102 86-87 -0.24117141500256167 1102 88-89 -0.1646174249779051 1102 90-91 -0.13784602312103544 1102 92-93 -0.2298647134141163 1102 94-95 -0.18843790348956801 1102 96-97 -0.16662651925775762 1102 98-99 -0.15651913314029287 1102 100-101 -0.05115923779018061 1103 1 0.20576763828459121 1103 2 0.22054965119159675 1103 3 0.27427962329122835 1103 4 0.18740586693105143 1103 5 0.2216550463290261 1103 6 0.20774134945394707 1103 7 0.22081365405491482 1103 8 0.1746041269986165 1103 9 0.2752927936096796 1103 10-11 0.2317541438453361 1103 12-13 0.25844240989864886 1103 14-15 0.3292865745377185 1103 16-17 0.3406950945494174 1103 18-19 0.3730356598034348 1103 20-21 0.32424575935488775 1103 22-23 0.35580605235870166 1103 24-25 0.3710610291963192 1103 26-27 0.36346018670701596 1103 28-29 0.37650759237141074 1103 30-31 0.35989543484379993 1103 32-33 0.28930567835017484 1103 34-35 0.28816824561962306 1103 36-37 0.2826562052326409 1103 38-39 0.2483877406769679 1103 40-41 0.13705769059743034 1103 42-43 0.14958287323469932 1103 44-45 0.1118613438728886 1103 46-47 0.11834961331495464 1103 48-49 0.2286285845570859 1103 50-51 0.16046448577163375 1103 52-53 0.1330513614062312 1103 54-55 0.0725134740036637 1103 56-57 0.09058096820452732 1103 58-59 0.07777365451246965 1103 60-61 0.027145158534501945 1103 62-63 0.08015585199042619 1103 64-65 0.031076984434598387 1103 66-67 -0.03306319047056405 1103 68-69 -0.09308167650646482 1103 70-71 -0.04814019296744121 1103 72-73 -0.07236779779967151 1103 74-75 -0.10713680849262275 1103 76-77 -0.126114232057855 1103 78-79 -0.2258854266010033 1103 80-81 -0.2523524961373589 1103 82-83 -0.2556177057400717 1103 84-85 -0.33652375836484794 1103 86-87 -0.3120708421936236 1103 88-89 -0.2828626982550304 1103 90-91 -0.26594704209651354 1103 92-93 -0.31079637452551623 1103 94-95 -0.2539883560947267 1103 96-97 -0.2803856908767308 1103 98-99 -0.2699099045122786 1103 100-101 -0.15817269813437917 1104 1 0.22536020765924292 1104 2 0.23502371509400177 1104 3 0.21981326553373037 1104 4 0.20013703745797784 1104 5 0.1934485658702414 1104 6 0.20309245446205892 1104 7 0.19754082003996842 1104 8 0.1709385117729525 1104 9 0.20855404076687023 1104 10-11 0.18106669933345643 1104 12-13 0.22337074385665545 1104 14-15 0.2698784993381267 1104 16-17 0.25729326829257104 1104 18-19 0.2974372173955331 1104 20-21 0.31742173033467935 1104 22-23 0.35221642060629677 1104 24-25 0.3525909919481123 1104 26-27 0.31062251217168324 1104 28-29 0.3318242795472628 1104 30-31 0.34973346759878154 1104 32-33 0.2592575177085621 1104 34-35 0.2809272334501074 1104 36-37 0.2898047878879524 1104 38-39 0.22254824629737158 1104 40-41 0.03280444036221297 1104 42-43 0.02914166050640432 1104 44-45 0.05123891512155865 1104 46-47 0.05129774996469649 1104 48-49 0.13565800942659934 1104 50-51 0.052971558951263376 1104 52-53 0.0456171593902468 1104 54-55 0.06924574240748171 1104 56-57 0.07311223226658825 1104 58-59 0.07578467861159055 1104 60-61 0.034772279520979055 1104 62-63 0.08144538744366514 1104 64-65 0.013025091232705677 1104 66-67 0.06995898376791132 1104 68-69 0.03518815576050116 1104 70-71 0.030049979652414294 1104 72-73 0.02719330020988764 1104 74-75 0.0063886643517854225 1104 76-77 0.0029778469760124437 1104 78-79 -0.06387887693064087 1104 80-81 -0.03738961167314514 1104 82-83 -0.06496143407690269 1104 84-85 -0.10097598634733274 1104 86-87 -0.05082614500531335 1104 88-89 -0.07306456166057274 1104 90-91 -0.01803278705518352 1104 92-93 -0.05257780415360003 1104 94-95 -0.01005050499248128 1104 96-97 -0.027310143016268995 1104 98-99 -0.006731567513828196 1104 100-101 0.06777954145707099 1105 1 0.08308736904294278 1105 2 0.11997013964718661 1105 3 0.15037049497388466 1105 4 0.020056164500488194 1105 5 0.0863826856420502 1105 6 0.11658178328651303 1105 7 0.08823467246865846 1105 8 0.09894145780985042 1105 9 0.15323843993962072 1105 10-11 0.06770217018517144 1105 12-13 0.1215577099065186 1105 14-15 0.21415669860650155 1105 16-17 0.08459911321616431 1105 18-19 0.17699603183730517 1105 20-21 0.09309111414697213 1105 22-23 0.1360730700674182 1105 24-25 0.18802790572939188 1105 26-27 0.19333309594171766 1105 28-29 0.2440939240047868 1105 30-31 0.25404446503818434 1105 32-33 0.08142312393562179 1105 34-35 0.06526447833807225 1105 36-37 0.0847141677040355 1105 38-39 0.06379892186077285 1105 40-41 -0.10796451994262668 1105 42-43 -0.11944676814922417 1105 44-45 -0.1527147739148944 1105 46-47 -0.12736027418075224 1105 48-49 -0.0863313640152441 1105 50-51 -0.11858860526883319 1105 52-53 -0.1563753173339606 1105 54-55 -0.1083662914801593 1105 56-57 -0.05301123451668133 1105 58-59 -0.07430385845079712 1105 60-61 -0.12429761580231258 1105 62-63 -0.0516708451093848 1105 64-65 -0.10730341638807772 1105 66-67 -0.12237649506919723 1105 68-69 -0.0864689589174148 1105 70-71 -0.12032821859579101 1105 72-73 -0.09900911764166409 1105 74-75 -0.11846517415581204 1105 76-77 -0.06796187476866677 1105 78-79 -0.12303814479286856 1105 80-81 -0.19670585825047482 1105 82-83 -0.20543273607300705 1105 84-85 -0.31266091741084523 1105 86-87 -0.3410295961508112 1105 88-89 -0.2515288461226888 1105 90-91 -0.2755848501428666 1105 92-93 -0.3456758942600686 1105 94-95 -0.32565337549057105 1105 96-97 -0.3675339739266015 1105 98-99 -0.3953514095014654 1105 100-101 -0.26918379677655224 1106 1 -0.011345414295995226 1106 2 -0.01272533612562654 1106 3 0.010580534670623365 1106 4 -0.1266371344042696 1106 5 0.01295154157572398 1106 6 0.02513820574441894 1106 7 -0.02076743354140831 1106 8 -0.0014929762218685028 1106 9 0.025265890751491327 1106 10-11 -0.0024111569422728962 1106 12-13 -0.011978681166027627 1106 14-15 -0.07093756570297671 1106 16-17 -0.05098610365800482 1106 18-19 -0.033121322658736574 1106 20-21 -0.1737024636232647 1106 22-23 -0.016215904087758304 1106 24-25 0.06286068450662441 1106 26-27 -0.09302790660677118 1106 28-29 0.0065017825865538725 1106 30-31 -0.026342011023409384 1106 32-33 -0.1714176018901412 1106 34-35 -0.14232637564917638 1106 36-37 -0.2207878477900067 1106 38-39 -0.1317024270774496 1106 40-41 -0.17882852919147751 1106 42-43 -0.21358069569338056 1106 44-45 -0.21928583555584424 1106 46-47 -0.16502763775806528 1106 48-49 -0.08219718512156504 1106 50-51 -0.20157077661780676 1106 52-53 -0.20728972719542327 1106 54-55 -0.14984491765361696 1106 56-57 -0.12129846871635408 1106 58-59 -0.1051676528363572 1106 60-61 -0.15506929904233147 1106 62-63 -0.08689471234168167 1106 64-65 -0.1321774267139233 1106 66-67 -0.13195540105673587 1106 68-69 -0.10964436014938883 1106 70-71 -0.08413050645615172 1106 72-73 -0.11961771526731724 1106 74-75 -0.1494636257463391 1106 76-77 -0.10133102845534481 1106 78-79 -0.2571384407329482 1106 80-81 -0.28378695570319934 1106 82-83 -0.2478310958634573 1106 84-85 -0.2924409914322439 1106 86-87 -0.3243937523911775 1106 88-89 -0.33634492035832864 1106 90-91 -0.27285782049326457 1106 92-93 -0.330862342449052 1106 94-95 -0.34046550971786615 1106 96-97 -0.42613468368718443 1106 98-99 -0.40765134756710353 1106 100-101 -0.2563892518273647 1107 1 -0.06493061247837417 1107 2 -0.10857834486284901 1107 3 -0.10035550352608169 1107 4 -0.06924009492097838 1107 5 -0.07037944188179779 1107 6 -0.05174279231042078 1107 7 -0.03767139668714492 1107 8 -0.08582740723809223 1107 9 -0.037413582277956436 1107 10-11 -0.07698255654261743 1107 12-13 -9.878731567525278E-4 1107 14-15 -0.06497353221405433 1107 16-17 -0.06539375503372469 1107 18-19 -0.05772885280110529 1107 20-21 -0.11304663268671078 1107 22-23 -0.03594800094427342 1107 24-25 -0.044347214383620326 1107 26-27 0.040662804262332486 1107 28-29 0.03304177635222061 1107 30-31 0.033082273779996285 1107 32-33 -0.18766356186805666 1107 34-35 -0.3038004080468326 1107 36-37 -0.24960519371926182 1107 38-39 -0.2711050176199947 1107 40-41 -0.3813651621996854 1107 42-43 -0.35583278256901707 1107 44-45 -0.36719707407895896 1107 46-47 -0.36420025913397325 1107 48-49 -0.3542363655089673 1107 50-51 -0.379917416243039 1107 52-53 -0.2768935898873792 1107 54-55 -0.2342751826253604 1107 56-57 -0.27081397112023353 1107 58-59 -0.2754435308107972 1107 60-61 -0.30391224688113994 1107 62-63 -0.3248541017424671 1107 64-65 -0.3983975393227013 1107 66-67 -0.3651660348280501 1107 68-69 -0.37021168904647084 1107 70-71 -0.33885490204368196 1107 72-73 -0.3155253018304798 1107 74-75 -0.3602650173240711 1107 76-77 -0.5531605891452749 1107 78-79 -0.5904129892290975 1107 80-81 -0.5064747412728678 1107 82-83 -0.5008958429704577 1107 84-85 -0.6444978499935132 1107 86-87 -0.684295680817641 1107 88-89 -0.6363094145949155 1107 90-91 -0.6256553572527714 1107 92-93 -0.7114046370997897 1107 94-95 -0.6791041739743982 1107 96-97 -0.7173671437677172 1107 98-99 -0.7679107378870924 1107 100-101 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-1.9885679898709832 2308 74-75 -1.970591552084091 2308 76-77 -1.8183802181672668 2308 78-79 -1.6003721819556311 2308 80-81 -1.8023456992325961 2308 82-83 -1.831443251932562 2308 84-85 -2.2480896842411298 2308 86-87 -2.153029794504622 2308 88-89 -2.337233274353597 2308 90-91 -2.7692698749699147 2308 92-93 -2.4204454033616685 2308 94-95 -3.4062344396136197 2308 96-97 -2.842162352338441 2308 98-99 -3.3005020800327145 2308 100-101 -3.097554504029958 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 130.0 11 1260.0 12 4402.0 13 9215.0 14 14610.0 15 21085.0 16 29585.0 17 45421.0 18 88917.0 19 221406.0 20 261309.0 21 211974.0 22 196056.0 23 187124.0 24 189284.0 25 199148.0 26 221150.0 27 248599.0 28 280640.0 29 311945.0 30 345529.0 31 402055.0 32 489469.0 33 630293.0 34 867429.0 35 1528275.0 36 2341567.0 37 2685097.0 38 2186832.0 39 295248.0 40 146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.745945113268146 39.9561714676792 15.900936135783711 15.396947283268947 2 17.84969992150299 21.392027475745774 46.718013293924535 14.040259308826705 3 17.71417304423321 36.646448323626494 18.108277101482983 27.53110153065731 4 29.419514795591546 18.675275755721486 35.45395372382693 16.451255724860047 5 17.78273564501548 30.786605656607463 17.164501052069408 34.26615764630764 6 18.042153323116 18.72276389953099 33.74645423467066 29.488628542682356 7 29.287928614427827 19.086575577797678 17.197053130917503 34.428442676856996 8 46.268849720451705 18.868790113978434 17.214483132925054 17.64787703264481 9 17.769563248241397 35.66997551945884 17.464620885055542 29.095840347244224 10-11 40.75974967485813 18.668007513777624 17.362469361432243 23.20977344993201 12-13 31.984887153298363 18.560925148777656 17.619246802687982 31.834940895235995 14-15 31.55657082829436 18.68565797430859 17.793948027729826 31.963823169667222 16-17 31.692108039557425 24.401279431040642 17.64175241929117 26.264860110110767 18-19 25.918209750026218 18.929340425300317 23.7228355485511 31.42961427612237 20-21 25.417296982845983 32.77887210939262 18.820409802081983 22.98342110567941 22-23 16.57106115367874 23.97121996648755 33.45667872896292 26.001040150870793 24-25 16.631590797014052 18.710469892186136 39.058240457142794 25.599698853657017 26-27 16.529101070040632 18.679340648264734 48.015478123220724 16.776080158473913 28-29 16.671101098007455 18.44291936137024 48.271271043971694 16.614708496650614 30-31 16.63317306998395 18.334841145139634 48.30760683621423 16.724378948662185 32-33 16.58446401168247 18.335818393690605 48.36483786341855 16.714879731208377 34-35 16.64882430564292 18.453970842190213 48.216592920384464 16.680611931782405 36-37 16.71503191906866 18.369322999107926 48.06984561803166 16.845799463791753 38-39 16.91539726439631 18.54585211624304 47.262653283148815 17.276097336211834 40-41 18.57287545273109 19.971663034897162 42.51045972352806 18.94500178884369 42-43 18.538182180690235 20.613959047327757 42.50461599321558 18.343242778766427 44-45 18.730112198265108 21.6842984951643 41.34235567613057 18.24323363044002 46-47 18.391176505035098 22.648081895349055 40.822866654128006 18.137874945487837 48-49 18.358015889162083 23.003047086508143 40.27083858502036 18.36809843930942 50-51 18.260475160112165 23.190075136853434 40.152405297671415 18.397044405362983 52-53 18.233455135537476 23.174660006642757 40.23126871719399 18.360616140625776 54-55 18.311129366352557 22.904764097871045 40.18794761601516 18.596158919761244 56-57 18.288539835683995 22.804904816343583 40.29954250722794 18.60701284074448 58-59 18.44790516255878 22.890921448491685 40.02714947931635 18.634023909633186 60-61 18.682614825677025 22.88899085128612 39.531730801359 18.89666352167786 62-63 18.95933656314256 23.06291018202847 38.933615942788805 19.044137312040164 64-65 19.140188334960822 23.308201291308244 38.22159691611595 19.33001345761499 66-67 19.300420104529067 23.389262302123768 37.61365415846578 19.696663434881387 68-69 19.438228156543804 23.681863728007176 37.10958642306687 19.770321692382144 70-71 19.69419352127338 23.917071169401304 36.694004403111734 19.694730906213582 72-73 19.85897892410355 23.941674517456725 36.39608932652984 19.80325723190989 74-75 19.84886876169582 24.060707131933317 36.182264146627695 19.908159959743163 76-77 19.944831994218603 24.137752713396786 35.894724225258464 20.02269106712614 78-79 20.120228302907567 23.992338263416663 35.736335040440025 20.15109839323575 80-81 20.113389010590385 24.071264527659032 35.59871427723817 20.216632184512406 82-83 20.281829209883934 24.203697931909485 35.33636715372037 20.178105704486217 84-85 20.377765795623446 24.077808051605828 35.163108884266045 20.38131726850468 86-87 20.441046447503993 24.194991559223755 35.00635706348827 20.357604929783978 88-89 20.63450037577024 24.29759280725775 34.6433706685362 20.424536148435806 90-91 20.837245613922683 24.200329158603562 34.35921029216908 20.603214935304674 92-93 20.851672157692924 24.306416626214812 34.174769782678815 20.66714143341345 94-95 20.94175993214284 24.46005896165646 33.85066588146657 20.747515224734133 96-97 20.98871955881486 24.435491368869467 33.595557393830795 20.98023167848488 98-99 21.093213059602984 24.45945646165183 33.5155009482531 20.93182953049208 100-101 21.282692581476915 24.56202034549919 32.99133208666321 21.16395498636068 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 175367.0 1 98771.5 2 15334.0 3 6667.5 4 4289.0 5 3359.5 6 2802.5 7 2596.0 8 2453.5 9 2389.0 10 3115.5 11 3963.5 12 6892.0 13 11484.5 14 15700.0 15 28890.5 16 834723.5 17 901233.0 18 145029.0 19 102155.5 20 75705.5 21 59135.5 22 52171.0 23 51520.0 24 53944.5 25 57606.0 26 63312.0 27 71474.0 28 81386.0 29 93986.0 30 109605.0 31 125629.0 32 144454.0 33 167973.0 34 192396.0 35 217330.0 36 245477.5 37 271400.5 38 293369.5 39 311567.0 40 320672.0 41 325964.0 42 334016.5 43 344536.0 44 363023.0 45 390324.0 46 419905.0 47 446395.5 48 470985.0 49 491339.0 50 486657.5 51 472780.0 52 476862.5 53 489721.5 54 487859.0 55 468075.0 56 435104.5 57 392797.5 58 351476.0 59 312281.5 60 272423.0 61 232789.0 62 193977.0 63 149368.5 64 105319.0 65 71162.0 66 48221.5 67 35151.5 68 26162.0 69 19496.5 70 15143.0 71 11255.5 72 8989.5 73 7096.0 74 5344.0 75 4629.0 76 3825.5 77 2334.0 78 1275.0 79 685.0 80 340.5 81 177.5 82 103.0 83 58.5 84 32.0 85 15.5 86 8.0 87 4.0 88 1.0 89 2.0 90 2.0 91 1.0 92 1.5 93 2.5 94 2.0 95 1.5 96 2.0 97 2.0 98 3.5 99 7.0 100 21.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.033399328510895E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.789130871206615E-5 28-29 0.0 30-31 0.001643104932332323 32-33 0.0031966485895270355 34-35 8.267194628087159E-4 36-37 9.128360735179573E-4 38-39 0.0016121029524769962 40-41 0.001646549596760693 42-43 0.001229745200927965 44-45 0.003406773119657584 46-47 6.338182548200156E-4 48-49 1.722332214184825E-4 50-51 0.0015225416773393854 52-53 9.576167110867627E-4 54-55 2.7901781869794164E-4 56-57 0.0 58-59 4.478063756880545E-5 60-61 3.2379845626674713E-4 62-63 0.0 64-65 0.0 66-67 3.44466442836965E-5 68-69 0.0032104272472405137 70-71 0.0032173165760972532 72-73 0.001436425066630144 74-75 0.00275573154269572 76-77 0.01682718573258574 78-79 0.030261377003227376 80-81 0.02639301885016826 82-83 0.001171185905645681 84-85 6.717095635320817E-4 86-87 0.001691330234329498 88-89 0.002400931106573646 90-91 7.57826174241323E-5 92-93 2.75573154269572E-5 94-95 4.13359731404358E-5 96-97 0.002676504260843218 98-99 6.200395971065369E-4 100-101 1.033399328510895E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4515202E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.48570361128683 #Duplication Level Percentage of deduplicated Percentage of total 1 74.28208953703744 24.873880338643062 2 12.827007642965274 8.590427523040923 3 4.441388669731559 4.461690738514755 4 2.06812659526395 2.770106967985136 5 1.2070666239106818 2.020973760367486 6 0.796995704023442 1.6012777154638718 7 0.5588772429864683 1.3100078399616608 8 0.41103252535381973 1.10109706548774 9 0.3141303993114018 0.9466989701973103 >10 2.6067264659894613 18.685128410731146 >50 0.3764479862443636 8.60021765745013 >100 0.10557348395203653 4.986394358421758 >500 0.0020998270306442756 0.49323981958607815 >1k 0.0018423766092436342 1.3240758318662815 >5k 3.262462269232977E-4 0.8109961666571152 >10k+ 2.686733633485981E-4 17.423786835625464 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 2136855 14.721496814167656 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 177805 1.2249571173725313 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 116520 0.8027445983872632 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 64321 0.4431285213943285 No Hit GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58629 0.40391446154176847 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27643 0.19044171758684447 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 23207 0.15988065477834892 No Hit GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19991 0.13772457317507536 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18992 0.1308421336471928 No Hit TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17300 0.11918538922158989 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.584545637050039E-4 0.0 0.0 0.0 0.0 2 1.653438925617432E-4 0.0 0.0 0.0 0.0 3 1.653438925617432E-4 0.0 0.0 0.0 0.0 4 1.653438925617432E-4 0.0 0.0 0.0 0.0 5 1.653438925617432E-4 0.0 0.0 0.0 0.0 6 1.722332214184825E-4 0.0 0.0 0.0 0.0 7 1.860118791319611E-4 0.0 0.0 0.0 0.0 8 1.860118791319611E-4 0.0 0.0 6.8893288567393E-6 0.0 9 1.929012079887004E-4 0.0 0.0 6.8893288567393E-6 0.0 10-11 2.06679865702179E-4 0.0 0.0 2.06679865702179E-5 6.8893288567393E-6 12-13 2.411265099858755E-4 0.0 0.0 2.06679865702179E-5 1.722332214184825E-5 14-15 3.1001979855326846E-4 0.0 0.0 2.06679865702179E-5 3.44466442836965E-5 16-17 3.4791110726533465E-4 0.0 0.0 2.06679865702179E-5 5.855929528228405E-5 18-19 4.236937246894669E-4 0.0 0.0 2.06679865702179E-5 9.645060399435021E-5 20-21 4.7880835554338136E-4 0.0 0.0 2.06679865702179E-5 1.033399328510895E-4 22-23 5.51146308539144E-4 0.0 0.0 2.06679865702179E-5 1.240079194213074E-4 24-25 6.234842615349067E-4 0.0 0.0 2.06679865702179E-5 1.3778657713478602E-4 26-27 6.78598892388821E-4 0.0 0.0 2.06679865702179E-5 1.446759059915253E-4 28-29 7.440475165278444E-4 0.0 0.0 2.06679865702179E-5 1.446759059915253E-4 30-31 7.957174829533891E-4 0.0 0.0 2.75573154269572E-5 1.5500989927663426E-4 32-33 8.542767782356731E-4 0.0 0.0 2.75573154269572E-5 1.791225502752218E-4 34-35 9.266147312314358E-4 0.0 0.0 2.75573154269572E-5 1.860118791319611E-4 36-37 9.817293620853502E-4 0.0 0.0 2.75573154269572E-5 2.0323520127380935E-4 38-39 0.0010575119795094825 0.0 0.0 2.75573154269572E-5 2.3079251670076655E-4 40-41 0.0011815198989307898 0.0 0.0 2.75573154269572E-5 2.411265099858755E-4 42-43 0.0012745258384967706 0.0 0.0 2.75573154269572E-5 2.480158388426148E-4 44-45 0.0013847551002045993 0.0 0.0 2.75573154269572E-5 2.652391609844631E-4 46-47 0.0015018736907691675 0.0 0.0 2.75573154269572E-5 2.686838254128327E-4 48-49 0.0015914349659067782 0.0 0.0 2.75573154269572E-5 2.7901781869794164E-4 50-51 0.0017188875497564553 0.0 0.0 2.75573154269572E-5 2.962411408397899E-4 52-53 0.002404375771002016 0.0 0.0 2.75573154269572E-5 2.962411408397899E-4 54-55 0.0033792158042306266 0.0 0.0 2.75573154269572E-5 3.0657513412489886E-4 56-57 0.004670964964869245 0.0 0.0 3.44466442836965E-5 3.375771139802257E-4 58-59 0.006262399930776024 0.0 0.0 3.44466442836965E-5 3.720237582639222E-4 60-61 0.008360200567653141 0.0 0.0 3.44466442836965E-5 4.0991506697598835E-4 62-63 0.011167602076774405 0.0 0.0 3.44466442836965E-5 4.374723824029455E-4 64-65 0.014698383115853298 0.0 0.0 3.44466442836965E-5 4.5814036897316344E-4 66-67 0.019245340161301236 0.0 0.0 3.44466442836965E-5 4.891423488284903E-4 68-69 0.024946259790253004 0.0 0.0 3.44466442836965E-5 4.994763421135993E-4 70-71 0.03228683968710873 0.0 0.0 3.44466442836965E-5 5.098103353987082E-4 72-73 0.041422089751145044 0.0 0.0 3.44466442836965E-5 5.201443286838172E-4 74-75 0.05333718400887566 0.0 0.0 3.44466442836965E-5 5.270336575405564E-4 76-77 0.06853159880241419 0.0 0.0 3.44466442836965E-5 5.339229863972957E-4 78-79 0.08707422742032801 0.0 0.0 3.44466442836965E-5 5.373676508256654E-4 80-81 0.11022237237897206 0.0 0.0 3.44466442836965E-5 5.442569796824047E-4 82-83 0.13927811683227004 0.0 0.0 3.44466442836965E-5 5.649249662526226E-4 84-85 0.17492350433703918 0.0 0.0 3.44466442836965E-5 5.718142951093619E-4 86-87 0.21812648559765135 0.0 0.0 3.7891308712066144E-5 5.855929528228405E-4 88-89 0.2702132564190288 0.0 0.0 4.13359731404358E-5 5.993716105363191E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 165870 0.0 94.611336 1 ATATCTC 257630 0.0 93.91368 1 TATCTCG 259115 0.0 93.23249 2 TCGACGG 169305 0.0 92.703 2 CGACGGC 172360 0.0 90.93589 3 ACGGCGC 173075 0.0 90.43672 5 GACGGCG 173500 0.0 90.31648 4 ATCTCGA 269080 0.0 89.71267 3 CGGCGCG 174740 0.0 89.56141 6 TCTCGAG 268540 0.0 89.13961 4 TCGAGGG 272815 0.0 87.67665 6 CTCGAGG 273130 0.0 87.596405 5 CGAGGGC 275690 0.0 86.7606 7 AGGGCGC 279450 0.0 85.79209 9 GAGGGCG 280900 0.0 85.36107 8 GGGCGCG 280785 0.0 42.76821 10-11 GGATCCT 280490 0.0 41.778572 18-19 CCATATT 179870 0.0 41.76867 18-19 TCCATAT 180345 0.0 41.69026 18-19 ATCCATA 179430 0.0 41.622276 16-17 >>END_MODULE