FastQCFastQC Report
Tue 31 May 2016
SRR1051260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1051260_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14515202
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13443459.261634801913194No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240830.8548485925307825No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1136090.7826897620852952No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT473320.3260857134471845No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368120.25360997387428713No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338400.23313488851205794No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT233600.16093472209343004No Hit
AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149290.10285079050226101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG192900.092.9365541
TCGACGG199050.090.0842362
CGACGGC237150.075.771473
ACGGCGC248600.072.127995
GACGGCG249700.071.924884
CGGCGCG268350.066.872636
ATCTCGA223150.064.3131561
TCTCGAG259700.054.8527642
TATCTCG55700.047.755882
CTCGAGG322300.044.287043
TCGAGGG323200.044.134174
ATATCTC63250.042.17741
CGAGGGC359600.039.758945
AGGGCGC411100.034.743537
GGGCGCG415450.034.3454488
CGCGCCG702400.032.7234549
GAGGGCG445550.032.163766
GGCGCGC724050.031.7712257
GCGCGCC734850.031.3172118
CCATATT340900.026.84293618-19
Produced by FastQC (version 0.11.5)