##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1051260_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14515202 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32850924155241 33.0 31.0 34.0 31.0 34.0 2 32.57677013382246 34.0 31.0 34.0 31.0 34.0 3 32.67601890762526 34.0 31.0 34.0 31.0 34.0 4 36.2017299518119 37.0 35.0 37.0 35.0 37.0 5 36.05856053536148 37.0 35.0 37.0 35.0 37.0 6 36.018403257495144 37.0 35.0 37.0 35.0 37.0 7 35.96089203581183 37.0 35.0 37.0 35.0 37.0 8 35.952116202034254 37.0 35.0 37.0 35.0 37.0 9 37.62868639375463 39.0 37.0 39.0 35.0 39.0 10-11 37.59116362968976 39.0 37.0 39.0 35.0 39.0 12-13 37.57821937304076 39.0 37.0 39.0 35.0 39.0 14-15 38.95630639518485 40.0 38.0 41.0 35.0 41.0 16-17 38.84755988928022 40.0 38.0 41.0 35.5 41.0 18-19 38.502273306289496 40.0 37.0 41.0 35.0 41.0 20-21 38.48809802991374 40.0 37.0 41.0 35.0 41.0 22-23 38.42978227240654 40.0 37.0 41.0 35.0 41.0 24-25 38.376834714391165 40.0 37.0 41.0 35.0 41.0 26-27 38.2342223001788 40.0 36.5 41.0 34.0 41.0 28-29 38.02697041350165 40.0 36.0 41.0 34.0 41.0 30-31 37.83759447508895 40.0 35.5 41.0 34.0 41.0 32-33 37.87605811479578 40.0 35.0 41.0 34.0 41.0 34-35 37.81241232467863 40.0 35.0 41.0 33.0 41.0 36-37 37.752459077042126 40.0 35.0 41.0 33.0 41.0 38-39 37.67150774064322 40.0 35.0 41.0 33.0 41.0 40-41 37.46449105565324 40.0 35.0 41.0 33.0 41.0 42-43 37.391130002875606 40.0 35.0 41.0 33.0 41.0 44-45 37.33019633484949 39.0 35.0 41.0 33.0 41.0 46-47 37.2200635581923 39.0 35.0 41.0 33.0 41.0 48-49 37.160854978111914 39.0 35.0 41.0 33.0 41.0 50-51 37.134455999992284 39.0 35.0 41.0 33.0 41.0 52-53 36.97806348130739 39.0 35.0 41.0 32.5 41.0 54-55 36.74268291271454 38.0 35.0 40.5 32.0 41.0 56-57 36.5674581380266 38.0 35.0 40.0 32.0 41.0 58-59 36.30916741634047 37.5 35.0 40.0 31.0 41.0 60-61 36.020570709246755 37.0 35.0 40.0 31.0 41.0 62-63 35.67835962599763 36.5 35.0 40.0 31.0 41.0 64-65 35.321801446511046 36.0 34.5 39.0 30.5 41.0 66-67 34.9315987817462 35.0 34.0 39.0 30.0 41.0 68-69 34.53235407953675 35.0 34.0 38.0 29.5 40.0 70-71 34.17776208005924 35.0 34.0 37.0 29.0 39.5 72-73 33.81188026181103 35.0 33.5 37.0 29.0 39.0 74-75 33.480585871281704 35.0 33.0 36.0 29.0 39.0 76-77 33.06961270673325 35.0 33.0 36.0 28.0 37.5 78-79 32.87881367410526 35.0 33.0 35.0 28.0 37.0 80-81 32.6443680907782 35.0 33.0 35.0 27.5 37.0 82-83 32.38179310215593 35.0 33.0 35.0 27.0 36.0 84-85 32.14784165594113 35.0 33.0 35.0 27.0 36.0 86-87 31.890626186256313 35.0 33.0 35.0 26.0 36.0 88-89 31.68919344009129 35.0 33.0 35.0 25.5 35.0 90-91 31.459754848744097 35.0 33.0 35.0 25.0 35.0 92-93 31.2007654802186 35.0 32.0 35.0 24.0 35.0 94-95 30.944916646699095 35.0 32.0 35.0 23.5 35.0 96-97 30.719307213223765 34.5 32.0 35.0 21.5 35.0 98-99 30.407400771963076 34.0 32.0 35.0 18.0 35.0 100-101 28.99457871822934 33.0 29.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.316107767712964 1101 2 0.33805048805901805 1101 3 0.3098653050967215 1101 4 0.207439137552079 1101 5 0.25325821099873025 1101 6 0.25227733322142143 1101 7 0.2950433454443342 1101 8 0.2982589470566168 1101 9 0.4056035758519059 1101 10-11 0.3831127121353006 1101 12-13 0.35027437917017323 1101 14-15 0.45731133479234387 1101 16-17 0.4403447456817844 1101 18-19 0.3686578976911292 1101 20-21 0.3340646238799323 1101 22-23 0.35251412670789506 1101 24-25 0.340236077633179 1101 26-27 0.3912645039301097 1101 28-29 0.39576935285044357 1101 30-31 0.420983886973886 1101 32-33 0.46369486083528244 1101 34-35 0.43341383198356453 1101 36-37 0.4292518477547489 1101 38-39 0.40789340735410917 1101 40-41 0.4155445700863183 1101 42-43 0.4062944228374832 1101 44-45 0.34992823941406215 1101 46-47 0.38686849518719413 1101 48-49 0.3501618065226424 1101 50-51 0.40288993749604884 1101 52-53 0.40805478895487113 1101 54-55 0.4496922375743182 1101 56-57 0.36592258821711 1101 58-59 0.3174125693873435 1101 60-61 0.29186878414991924 1101 62-63 0.3303841275005084 1101 64-65 0.34813929654525566 1101 66-67 0.2674493531348219 1101 68-69 0.26156633845198485 1101 70-71 0.25800973804906135 1101 72-73 0.21674666543277965 1101 74-75 0.2094792977041493 1101 76-77 0.1496029746917742 1101 78-79 0.17902076166087255 1101 80-81 0.18930850597676852 1101 82-83 0.03963522501574346 1101 84-85 0.09906124241514647 1101 86-87 0.18212879154366846 1101 88-89 0.1512159594850715 1101 90-91 0.11767523351438669 1101 92-93 0.02366536116593565 1101 94-95 0.05618670524653524 1101 96-97 0.05228841979389287 1101 98-99 0.050705808656186235 1101 100-101 0.029597870204494114 1102 1 0.3113172097837662 1102 2 0.30294660713509103 1102 3 0.287070094917766 1102 4 0.18296485874302704 1102 5 0.2470160380977049 1102 6 0.2534317804527788 1102 7 0.26588690465103326 1102 8 0.25123517634221315 1102 9 0.3306754116026056 1102 10-11 0.3237318380137424 1102 12-13 0.30026806181893306 1102 14-15 0.37867807110643525 1102 16-17 0.3532700748584645 1102 18-19 0.30836792492064546 1102 20-21 0.3199548053072263 1102 22-23 0.2876986637925185 1102 24-25 0.32886995556798126 1102 26-27 0.3524352389118022 1102 28-29 0.3711851462959217 1102 30-31 0.36454757177225616 1102 32-33 0.3373978699725413 1102 34-35 0.3826273024900644 1102 36-37 0.3227493299130231 1102 38-39 0.3480509199990891 1102 40-41 0.3461565687558945 1102 42-43 0.31755551236737745 1102 44-45 0.2976346927501865 1102 46-47 0.27185565790332333 1102 48-49 0.2891527353029417 1102 50-51 0.3444813230567547 1102 52-53 0.34944136327116837 1102 54-55 0.38535616348588775 1102 56-57 0.33002492420946083 1102 58-59 0.3196933353441267 1102 60-61 0.2923689942327812 1102 62-63 0.2841015150346351 1102 64-65 0.29267435111530915 1102 66-67 0.2895425424124767 1102 68-69 0.2833177474690203 1102 70-71 0.2744746227030319 1102 72-73 0.24310383905317678 1102 74-75 0.23631427219535084 1102 76-77 0.1542923838707253 1102 78-79 0.21073681148077839 1102 80-81 0.2115769772221583 1102 82-83 0.1137159143893669 1102 84-85 0.10191429497270832 1102 86-87 0.24213436027810076 1102 88-89 0.2588842391356003 1102 90-91 0.14849642325474122 1102 92-93 0.12581317604110964 1102 94-95 0.11190988619372177 1102 96-97 0.06905315049941407 1102 98-99 0.11099978719421344 1102 100-101 0.14894424602577416 1103 1 0.23315766785926684 1103 2 0.26710137427929226 1103 3 0.23587690148549 1103 4 0.13731216085766818 1103 5 0.18291428124893372 1103 6 0.19744484387153705 1103 7 0.21736841801696727 1103 8 0.1989749715891449 1103 9 0.2437361747219171 1103 10-11 0.2060365025757207 1103 12-13 0.23193002427063192 1103 14-15 0.29682161560748455 1103 16-17 0.264411359627033 1103 18-19 0.23927630409056633 1103 20-21 0.2501996313150485 1103 22-23 0.21249957207262327 1103 24-25 0.1704753438578308 1103 26-27 0.18217284684950386 1103 28-29 0.1940741541761426 1103 30-31 0.18908354334725175 1103 32-33 0.16169283226420106 1103 34-35 0.17564882753840294 1103 36-37 0.1877271572747219 1103 38-39 0.18601555509768275 1103 40-41 0.13345993984005844 1103 42-43 0.16939910842414463 1103 44-45 0.1363357957136202 1103 46-47 0.1816449191294751 1103 48-49 0.21184120287838226 1103 50-51 0.22174816211281012 1103 52-53 0.22212280637863557 1103 54-55 0.21548039734960156 1103 56-57 0.22973997881332053 1103 58-59 0.19786541259219348 1103 60-61 0.15100555437913243 1103 62-63 0.13422475472680873 1103 64-65 0.14399436644573882 1103 66-67 0.13420636565746236 1103 68-69 0.13951641178146446 1103 70-71 0.12124167642175365 1103 72-73 0.08777165384772445 1103 74-75 0.015888469030549857 1103 76-77 -0.047228047103473614 1103 78-79 0.030720554127150024 1103 80-81 0.07290466435880205 1103 82-83 -0.015412410177361835 1103 84-85 0.021090982556408733 1103 86-87 0.13355864429269104 1103 88-89 0.07824524760815166 1103 90-91 0.010085501449758283 1103 92-93 -0.041347268081469934 1103 94-95 -0.04142904741550524 1103 96-97 -0.03398780104907928 1103 98-99 0.003103392789700621 1103 100-101 0.10071205268499384 1104 1 0.29392670131853293 1104 2 0.315590107497421 1104 3 0.2709654558149168 1104 4 0.17588373789755707 1104 5 0.21966092819140215 1104 6 0.22527231909744216 1104 7 0.26437639946030345 1104 8 0.2344219114093491 1104 9 0.2998125860686329 1104 10-11 0.2979104999200288 1104 12-13 0.28818673397893946 1104 14-15 0.3674388977154095 1104 16-17 0.3318138415142755 1104 18-19 0.32351425259760447 1104 20-21 0.3120970013857942 1104 22-23 0.3243784783528483 1104 24-25 0.29905953045046374 1104 26-27 0.33165359420183194 1104 28-29 0.30827283672388006 1104 30-31 0.3124254507341959 1104 32-33 0.2961791298098504 1104 34-35 0.2895434932719354 1104 36-37 0.2846821797021448 1104 38-39 0.27670767636608673 1104 40-41 0.27031322346488906 1104 42-43 0.2489476687460126 1104 44-45 0.21745706743759996 1104 46-47 0.23228111833055465 1104 48-49 0.2622102022846562 1104 50-51 0.3058844492103816 1104 52-53 0.29540304802210926 1104 54-55 0.28146398668837236 1104 56-57 0.23721623846950024 1104 58-59 0.27922911830811614 1104 60-61 0.13911393351261125 1104 62-63 0.16382843001618141 1104 64-65 0.11221821060692605 1104 66-67 0.0027401693199209376 1104 68-69 0.07617513459573644 1104 70-71 0.1331457078300886 1104 72-73 0.17147630838135797 1104 74-75 0.17014296308398968 1104 76-77 0.06115053357278555 1104 78-79 0.12604622996268944 1104 80-81 0.1360804584399915 1104 82-83 0.05136415473944567 1104 84-85 0.017775101374773783 1104 86-87 0.13241596231102903 1104 88-89 0.14832727327392448 1104 90-91 0.10776600158630245 1104 92-93 0.004236237451586078 1104 94-95 0.03744829331218824 1104 96-97 0.10091976390863522 1104 98-99 0.15062338509461526 1104 100-101 0.1829789915543678 1105 1 -0.06907792798374857 1105 2 0.06870754899969711 1105 3 0.09509326226859116 1105 4 0.06126819254595972 1105 5 0.06704552308260503 1105 6 0.10746795029968581 1105 7 0.12482530892594923 1105 8 0.08124851936165811 1105 9 0.1544321440528762 1105 10-11 0.10740599286116748 1105 12-13 0.12745421393658773 1105 14-15 0.15519378109050308 1105 16-17 0.11144990675625621 1105 18-19 0.043845125936186946 1105 20-21 0.03663189598734817 1105 22-23 0.060789798174802456 1105 24-25 0.029759837448523285 1105 26-27 0.03868606709399103 1105 28-29 0.008137149469071403 1105 30-31 0.00862003386775001 1105 32-33 0.06833704965264786 1105 34-35 0.02658931502732287 1105 36-37 0.005673167460564343 1105 38-39 -0.016101297812937787 1105 40-41 -0.016004959776402927 1105 42-43 -0.057261065308260584 1105 44-45 -0.09249205409251715 1105 46-47 -0.16688465100498462 1105 48-49 -0.013512783944221951 1105 50-51 -0.02017735060896797 1105 52-53 -0.026798571641521107 1105 54-55 -0.02983955959005158 1105 56-57 -0.094346857833564 1105 58-59 -0.026844488407213873 1105 60-61 -0.0538450365564529 1105 62-63 -0.03572225948942531 1105 64-65 -0.003117251696551193 1105 66-67 0.019671145589228445 1105 68-69 -0.028655505806121084 1105 70-71 -0.06461008196377094 1105 72-73 -2.5059350625156185E-4 1105 74-75 0.006651857772553171 1105 76-77 -0.07919236300623567 1105 78-79 0.009570260085816074 1105 80-81 0.036470875166315864 1105 82-83 -0.02043638048148466 1105 84-85 -0.044961551022538515 1105 86-87 0.04277019029727569 1105 88-89 0.029376085390435236 1105 90-91 -0.04031698427672836 1105 92-93 -0.1634211079937522 1105 94-95 -0.17363463145845515 1105 96-97 -0.16291298681905175 1105 98-99 -0.1123136790724466 1105 100-101 -0.04550297569565842 1106 1 -0.04794843193349152 1106 2 0.04640378753854435 1106 3 0.04601544075380559 1106 4 0.023155130710698302 1106 5 -0.13276329044821011 1106 6 -0.06263450078100163 1106 7 -0.010425785452653713 1106 8 -0.12202183356215812 1106 9 -0.18969875489652566 1106 10-11 0.058395288636148734 1106 12-13 0.06779081055294256 1106 14-15 -0.01363101171956771 1106 16-17 0.059912156186477716 1106 18-19 -0.1086377975191084 1106 20-21 -0.09338166109443335 1106 22-23 -0.06847102658854709 1106 24-25 -0.061092259248155756 1106 26-27 -0.059430532102844325 1106 28-29 -0.13240481515050817 1106 30-31 -0.13045546291571952 1106 32-33 -0.13907242184792779 1106 34-35 -0.13675807737140389 1106 36-37 -0.1505676631243631 1106 38-39 -0.154650027110236 1106 40-41 -0.1633174724985551 1106 42-43 -0.27436685184949994 1106 44-45 -0.21214260386037154 1106 46-47 -0.25198436188308904 1106 48-49 -0.15914001352188478 1106 50-51 -0.12770811534207382 1106 52-53 -0.19535506619746457 1106 54-55 -0.16149336304905404 1106 56-57 -0.21528187358600093 1106 58-59 -0.17411659138185342 1106 60-61 -0.20473472442025553 1106 62-63 -0.19940472032276801 1106 64-65 -0.16547469305216111 1106 66-67 -0.18329726928885037 1106 68-69 -0.185695847647402 1106 70-71 -0.17834835041848152 1106 72-73 -0.14000556930062658 1106 74-75 -0.1962956060130452 1106 76-77 -0.3140393979327456 1106 78-79 -0.23630888432022346 1106 80-81 -0.26724578703377233 1106 82-83 -0.42400226503250593 1106 84-85 -0.40846032731922577 1106 86-87 -0.29544021595216563 1106 88-89 -0.27368561603530495 1106 90-91 -0.38534731094996744 1106 92-93 -0.5174747518365344 1106 94-95 -0.47637691616366595 1106 96-97 -0.49862395648516156 1106 98-99 -0.4473220929220183 1106 100-101 -0.4249094803710314 1107 1 -0.047277708070915025 1107 2 -0.009457094623250839 1107 3 -0.03346542043353651 1107 4 -0.0358204346837141 1107 5 -0.012702454313625822 1107 6 -0.02236540883811955 1107 7 0.02519587439429216 1107 8 -0.014340971576515926 1107 9 -0.03892372917828624 1107 10-11 -0.03650097664021246 1107 12-13 -0.03844432638772588 1107 14-15 -0.027354940928837834 1107 16-17 -0.09338597342721755 1107 18-19 -0.18114461938057502 1107 20-21 -0.19607892531437443 1107 22-23 -0.24032139597258606 1107 24-25 -0.2574424154511519 1107 26-27 -0.25231640666346067 1107 28-29 -0.26480851054952126 1107 30-31 -0.3418084062636666 1107 32-33 -0.37192342217741015 1107 34-35 -0.3480457547526967 1107 36-37 -0.3755746477444575 1107 38-39 -0.2945859427086859 1107 40-41 -0.43736539767684945 1107 42-43 -0.46771270131950615 1107 44-45 -0.483743588058168 1107 46-47 -0.42214301355844697 1107 48-49 -0.34661529018510606 1107 50-51 -0.2727258175964806 1107 52-53 -0.25447291733080135 1107 54-55 -0.3098005594397435 1107 56-57 -0.4074602091414974 1107 58-59 -0.3363195070682963 1107 60-61 -0.40734614810700265 1107 62-63 -0.3188228470856558 1107 64-65 -0.3008123955761022 1107 66-67 -0.36404801335336856 1107 68-69 -0.36498873027931467 1107 70-71 -0.3846658096552602 1107 72-73 -0.3643381376671755 1107 74-75 -0.3396193349056489 1107 76-77 -0.5272949122831037 1107 78-79 -0.4282763811264303 1107 80-81 -0.3841154671609317 1107 82-83 -0.467223392251384 1107 84-85 -0.5363723242780338 1107 86-87 -0.4250217256844877 1107 88-89 -0.41609838547088884 1107 90-91 -0.5213642749234779 1107 92-93 -0.6708032268459014 1107 94-95 -0.6624153670828363 1107 96-97 -0.7394747738430425 1107 98-99 -0.7569187173388308 1107 100-101 -0.6986323221114077 1108 1 -0.1559018962569212 1108 2 -0.09576005724438375 1108 3 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68-69 -0.9949717635880759 2308 70-71 -0.974046652937723 2308 72-73 -1.074444428880284 2308 74-75 -0.9987720862614111 2308 76-77 -2.1719549984189364 2308 78-79 -1.6218358562655908 2308 80-81 -1.1707589819631998 2308 82-83 -1.1530388103768736 2308 84-85 -1.1047871162944887 2308 86-87 -1.6062724699697526 2308 88-89 -1.662566913534711 2308 90-91 -2.3798238947211985 2308 92-93 -1.5994383814392954 2308 94-95 -1.528814430654009 2308 96-97 -1.9017517781904019 2308 98-99 -2.5779261041899026 2308 100-101 -1.9393984778457245 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 17.0 12 151.0 13 1131.0 14 4118.0 15 9252.0 16 15719.0 17 23464.0 18 36205.0 19 56505.0 20 59552.0 21 61921.0 22 67127.0 23 75197.0 24 88054.0 25 105806.0 26 129065.0 27 161014.0 28 198913.0 29 237554.0 30 292947.0 31 374084.0 32 475794.0 33 633555.0 34 976843.0 35 1938058.0 36 1854488.0 37 3311431.0 38 2914314.0 39 412687.0 40 235.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 23.83196991255301 36.042194430401395 20.648689881488952 19.47714577555664 2 22.36250725966629 34.08690104738986 25.334860934762553 18.21573075818129 3 21.877202650493626 31.734853084525355 24.088280526673348 22.29966373830767 4 23.97544723330123 31.425003343050285 24.153481453597532 20.446067970050954 5 22.516641518319897 33.743257586081135 22.236845205461144 21.50325569013783 6 23.137163368446405 31.63486116142235 21.44878865619645 23.779186813934796 7 23.816251403184054 32.34025265373503 22.413398036072802 21.430097907008115 8 23.76854969017999 31.491170429457338 22.418482360769076 22.321797519593595 9 22.661710116056256 30.923145265219183 22.767440646020635 23.64770397270393 10-11 22.961337362029134 31.611134312839738 22.565404187967896 22.86212413716323 12-13 22.86793184138946 31.470130419128857 22.966070330953713 22.69586740852797 14-15 22.24600456817618 31.750622554202142 23.142974517337063 22.860398360284616 16-17 22.549920421362376 32.49834897233948 22.989018685375513 21.962711920922633 18-19 21.488071609337574 32.1426494788016 24.404613866207306 21.964665045653515 20-21 20.882582274776475 32.13632851957555 24.4495150670311 22.53157413861688 22-23 20.81260736157857 32.08651522727689 25.59053949094198 21.510337920202556 24-25 20.879872043112872 31.29268994302284 26.80786760004675 21.019570413817544 26-27 20.77383766343727 31.33819288219344 26.983034752117124 20.904934702252163 28-29 20.9215862100989 31.49879002717289 26.85391150601969 20.72571225670852 30-31 20.908630827183803 31.398822420797174 26.8590853919911 20.833461360027922 32-33 20.828259916741082 31.438332721790573 26.902140252681296 20.83126710878705 34-35 20.91998099647528 31.60745541123024 26.685267624935566 20.787295967358908 36-37 20.9747442715575 31.5768771250996 26.491209009698935 20.95716959364396 38-39 20.895189746584304 31.707402349619386 26.420638169554927 20.976769734241383 40-41 21.15203770502126 32.08579184774693 25.728453520660615 21.03371692657119 42-43 21.14278533636666 32.15267345228816 25.594631752282883 21.109909459062298 44-45 21.060988334850595 32.46059545020455 25.482273687958322 20.99614252698653 46-47 21.139454345864426 32.71274833102564 25.219693807912556 20.928103515197378 48-49 21.104993922923015 32.64583227984013 25.251970313606382 20.99720348363047 50-51 20.995860064503407 32.670130942717854 25.351186983136714 20.982822009642028 52-53 21.037674845999387 32.81724911578909 25.250547667197466 20.894528371014058 54-55 21.066258671426 32.74320605390128 25.200045442013142 20.99048983265958 56-57 20.951751136498135 32.78164850892189 25.36685676162137 20.89974359295861 58-59 21.03445752942329 32.90925954733527 25.168326971956713 20.887955951284727 60-61 21.04384079532617 32.78817614801365 25.103364045502087 21.064619011158094 62-63 21.052228553209247 32.87720005550043 25.088534765137954 20.98203662615236 64-65 21.07595540179186 33.026168013369706 24.948474709480447 20.949401875357985 66-67 21.08127740833369 32.8694977858386 24.899119557550765 21.150105248276944 68-69 21.057051083408968 33.01002631585837 24.82216230955656 21.110760291176106 70-71 21.16820007051917 33.10290135817607 24.7210235172752 21.00787505402956 72-73 21.156030270200556 33.01072437895187 24.69468986630187 21.138555484545698 74-75 21.049004347304294 33.090045870529394 24.725625588951498 21.13532419321481 76-77 21.138503618482197 33.176062585970215 24.55763274944434 21.12780104610325 78-79 21.17688062487866 33.031772482394665 24.551380683506853 21.239966209219823 80-81 21.03907381903936 33.10661180881414 24.597932220881855 21.25638215126465 82-83 21.15147356484333 33.1919740353865 24.44785238034218 21.20870001942799 84-85 21.13711886338199 33.0336946051457 24.41202333939273 21.417163192079585 86-87 21.128672546203628 33.121257974914855 24.426880177072285 21.323189301809233 88-89 21.18229563736006 33.20583137596018 24.279345199605213 21.332527787074547 90-91 21.254099667369424 33.0562916037958 24.21763403637097 21.471974692463807 92-93 21.185726523130715 33.06812058144282 24.23857759609546 21.507575299331005 94-95 21.278137268711014 33.19179551176055 24.10796019607444 21.422107023454 96-97 21.19338745681941 33.1184712413923 24.06369887239599 21.624442429392303 98-99 21.23915323852397 33.11699518062561 24.074529791675765 21.56932178917466 100-101 21.2209208698785 33.174924671204145 23.80332767730819 21.800826781609167 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1192132.0 1 661739.0 2 88789.0 3 38743.5 4 27604.0 5 21957.0 6 19028.0 7 17463.0 8 16524.5 9 16325.0 10 17360.0 11 18941.5 12 25322.0 13 39017.0 14 52233.0 15 51643.0 16 130955.5 17 132947.5 18 42168.0 19 30591.0 20 24318.0 21 21351.0 22 21299.0 23 23119.5 24 26026.0 25 29732.0 26 34895.5 27 41462.0 28 48782.0 29 57994.0 30 70324.0 31 82612.5 32 96075.5 33 112241.5 34 128485.5 35 147519.5 36 173940.5 37 200612.5 38 224798.0 39 250489.5 40 271844.5 41 289867.5 42 310550.5 43 331946.0 44 357960.0 45 393372.0 46 436282.0 47 479729.5 48 518327.5 49 550497.5 50 556285.0 51 549772.5 52 557920.0 53 573430.0 54 575873.5 55 557774.0 56 519127.5 57 471034.5 58 426443.5 59 383516.0 60 336605.5 61 288356.5 62 243176.5 63 190352.5 64 135856.0 65 92010.5 66 62729.0 67 46574.5 68 35055.0 69 26392.5 70 20834.5 71 15347.0 72 12154.0 73 9705.0 74 7509.0 75 6712.0 76 5535.0 77 3363.5 78 1848.0 79 985.5 80 456.5 81 222.0 82 158.0 83 92.5 84 54.0 85 40.5 86 18.0 87 2.0 88 2.5 89 3.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1111386531169184 2 4.960316776852296E-4 3 1.3089724827804669E-4 4 1.0333993285108949E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.44466442836965E-6 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.722332214184825E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.716048319548016E-4 82-83 4.271383891178366E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 3.44466442836965E-6 96-97 0.0 98-99 2.75573154269572E-4 100-101 1.722332214184825E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4515202E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.636864851945454 #Duplication Level Percentage of deduplicated Percentage of total 1 74.37042050040063 26.50328624355131 2 13.474641477257737 9.603879545069054 3 4.47324310627596 4.782370800847592 4 1.9970595216944942 2.846757611036701 5 1.06026014857591 1.8892173811351658 6 0.6730351511731255 1.4390917633779212 7 0.4538790169776279 1.1322377631015907 8 0.3301060778722751 0.941115654715204 9 0.2583731621412105 0.8286848514536496 >10 2.2907283267397274 18.80003435952784 >50 0.4835625052735585 11.837475683179507 >100 0.13316257364473155 6.456719843195392 >500 4.926645764059845E-4 0.11580345030914269 >1k 6.967939018778844E-4 0.5922857807766435 >5k 1.1299116488030315E-4 0.27004062966623027 >10k+ 2.259823297606063E-4 11.960998639057124 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1344345 9.261634801913194 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 124083 0.8548485925307825 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 113609 0.7826897620852952 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47332 0.3260857134471845 No Hit GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36812 0.25360997387428713 No Hit TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33840 0.23313488851205794 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 23360 0.16093472209343004 No Hit AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14929 0.10285079050226101 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.584545637050039E-4 0.0 0.0 0.0 0.0 2 1.584545637050039E-4 0.0 0.0 0.0 6.8893288567393E-6 3 1.584545637050039E-4 0.0 0.0 0.0 6.8893288567393E-6 4 1.584545637050039E-4 0.0 0.0 6.8893288567393E-6 2.06679865702179E-5 5 1.584545637050039E-4 0.0 0.0 6.8893288567393E-6 2.75573154269572E-5 6 1.584545637050039E-4 0.0 0.0 6.8893288567393E-6 2.75573154269572E-5 7 1.584545637050039E-4 0.0 0.0 2.06679865702179E-5 2.75573154269572E-5 8 1.584545637050039E-4 0.0 0.0 2.06679865702179E-5 3.44466442836965E-5 9 1.722332214184825E-4 0.0 0.0 2.06679865702179E-5 3.44466442836965E-5 10-11 1.7567788584685215E-4 0.0 0.0 2.06679865702179E-5 4.478063756880545E-5 12-13 1.860118791319611E-4 0.0 0.0 2.06679865702179E-5 7.233795299576265E-5 14-15 2.342371811291362E-4 0.0 0.0 2.06679865702179E-5 9.300593956598055E-5 16-17 2.652391609844631E-4 0.0 0.0 2.06679865702179E-5 1.171185905645681E-4 18-19 3.582451005504436E-4 0.0 0.0 2.06679865702179E-5 1.3778657713478602E-4 20-21 4.374723824029455E-4 0.0 0.0 3.100197985532685E-5 1.6189922813337354E-4 22-23 5.063656709703386E-4 0.0 0.0 3.44466442836965E-5 1.7567788584685215E-4 24-25 5.649249662526226E-4 0.0 0.0 3.44466442836965E-5 1.8945654356033076E-4 26-27 6.20039597106537E-4 0.0 0.0 3.44466442836965E-5 2.06679865702179E-4 28-29 6.751542279604514E-4 3.44466442836965E-6 0.0 3.44466442836965E-5 2.06679865702179E-4 30-31 7.061562078157783E-4 6.8893288567393E-6 0.0 3.7891308712066144E-5 2.3079251670076655E-4 32-33 7.612708386696926E-4 6.8893288567393E-6 0.0 4.13359731404358E-5 2.5834983212772377E-4 34-35 8.16385469523607E-4 1.033399328510895E-5 0.0 4.13359731404358E-5 2.617944965560934E-4 36-37 8.646107715207822E-4 1.37786577134786E-5 0.0 4.13359731404358E-5 2.6868382541283273E-4 38-39 9.369487245165448E-4 1.37786577134786E-5 0.0 4.13359731404358E-5 2.893518119830506E-4 40-41 0.001043733321796004 1.37786577134786E-5 0.0 4.13359731404358E-5 2.9279647641142025E-4 42-43 0.0011229606036485059 1.37786577134786E-5 0.0 4.13359731404358E-5 3.1690912741000777E-4 44-45 0.001191853892215899 1.37786577134786E-5 0.0 4.82253019971751E-5 3.5480043612207395E-4 46-47 0.0012848598317818794 1.722332214184825E-5 0.0 4.82253019971751E-5 3.6857909383555256E-4 48-49 0.0013744211069194904 2.06679865702179E-5 0.0 4.82253019971751E-5 3.7546842269229187E-4 50-51 0.0014915396974840584 2.06679865702179E-5 0.0 4.82253019971751E-5 3.858024159774008E-4 52-53 0.0021873619120147277 2.06679865702179E-5 0.0 4.82253019971751E-5 4.064704025476187E-4 54-55 0.0031794252673851865 2.06679865702179E-5 0.0 4.82253019971751E-5 4.13359731404358E-4 56-57 0.0045159550655926115 2.06679865702179E-5 0.0 5.1669966425544743E-5 4.202490602610973E-4 58-59 0.006145281340211455 2.06679865702179E-5 0.0 5.51146308539144E-5 4.340277179745759E-4 60-61 0.008274083956943899 2.06679865702179E-5 0.0 6.20039597106537E-5 4.5125104011642413E-4 62-63 0.011140044761347449 2.06679865702179E-5 0.0 6.20039597106537E-5 4.6502969782990275E-4 64-65 0.014677715129283078 2.06679865702179E-5 0.0 6.20039597106537E-5 4.960316776852296E-4 66-67 0.01926256348344308 2.06679865702179E-5 0.0 6.20039597106537E-5 4.960316776852296E-4 68-69 0.024991040427821812 2.06679865702179E-5 0.0 6.8893288567393E-5 4.994763421135993E-4 70-71 0.0322902843515371 2.06679865702179E-5 0.0 6.8893288567393E-5 5.201443286838172E-4 72-73 0.04140486642900319 2.411265099858755E-5 0.0 6.8893288567393E-5 5.477016441107743E-4 74-75 0.053278624713593375 2.75573154269572E-5 0.0 6.8893288567393E-5 5.752589595377315E-4 76-77 0.06845237152056169 2.75573154269572E-5 0.0 6.8893288567393E-5 5.890376172512102E-4 78-79 0.0869743321519053 2.75573154269572E-5 0.0 7.233795299576265E-5 5.924822816795798E-4 80-81 0.10998813519784292 3.44466442836965E-5 0.0 7.57826174241323E-5 6.028162749646888E-4 82-83 0.13886820176529407 3.44466442836965E-5 0.0 7.57826174241323E-5 6.20039597106537E-4 84-85 0.1743103540687894 3.44466442836965E-5 0.0 7.57826174241323E-5 6.338182548200156E-4 86-87 0.21722742818184687 3.44466442836965E-5 0.0 7.57826174241323E-5 6.475969125334941E-4 88-89 0.2689146179295335 3.44466442836965E-5 0.0 8.26719462808716E-5 6.682648991037121E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 19290 0.0 92.936554 1 TCGACGG 19905 0.0 90.084236 2 CGACGGC 23715 0.0 75.77147 3 ACGGCGC 24860 0.0 72.12799 5 GACGGCG 24970 0.0 71.92488 4 CGGCGCG 26835 0.0 66.87263 6 ATCTCGA 22315 0.0 64.313156 1 TCTCGAG 25970 0.0 54.852764 2 TATCTCG 5570 0.0 47.75588 2 CTCGAGG 32230 0.0 44.28704 3 TCGAGGG 32320 0.0 44.13417 4 ATATCTC 6325 0.0 42.1774 1 CGAGGGC 35960 0.0 39.75894 5 AGGGCGC 41110 0.0 34.74353 7 GGGCGCG 41545 0.0 34.345448 8 CGCGCCG 70240 0.0 32.723454 9 GAGGGCG 44555 0.0 32.16376 6 GGCGCGC 72405 0.0 31.771225 7 GCGCGCC 73485 0.0 31.317211 8 CCATATT 34090 0.0 26.842936 18-19 >>END_MODULE