##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033293_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20113204
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.508568301698723	33.0	33.0	33.0	27.0	33.0
2	30.457861412831093	33.0	33.0	33.0	27.0	33.0
3	30.45191681046938	33.0	33.0	33.0	27.0	33.0
4	34.05682764416848	37.0	37.0	37.0	27.0	37.0
5	34.08102095518944	37.0	37.0	37.0	27.0	37.0
6	34.15770391430426	37.0	37.0	37.0	27.0	37.0
7	33.8869996048367	37.0	37.0	37.0	27.0	37.0
8	34.03622366680117	37.0	37.0	37.0	27.0	37.0
9	33.77640777670231	37.0	37.0	37.0	27.0	37.0
10-11	33.876458395191534	37.0	37.0	37.0	27.0	37.0
12-13	33.80687194342582	37.0	37.0	37.0	27.0	37.0
14-15	35.134137181724	37.0	37.0	40.0	27.0	40.0
16-17	34.970500373784304	37.0	37.0	40.0	27.0	40.0
18-19	34.929972643841324	37.0	37.0	40.0	27.0	40.0
20-21	34.827684465389005	37.0	37.0	40.0	27.0	40.0
22-23	34.809512721096056	37.0	37.0	40.0	27.0	40.0
24-25	33.86789479189889	37.0	35.0	40.0	18.5	40.0
26-27	32.86187531335136	37.0	33.0	40.0	15.0	40.0
28-29	32.43096940199085	37.0	33.0	40.0	10.5	40.0
30-31	32.36529140260299	37.0	33.0	40.0	6.0	40.0
32-33	32.22671261127765	37.0	33.0	40.0	6.0	40.0
34-35	32.03530243117904	37.0	33.0	40.0	6.0	40.0
36-37	31.930717850820784	37.0	33.0	40.0	6.0	40.0
38-39	31.693905207743132	37.0	33.0	40.0	4.0	40.0
40-41	31.362246586869006	37.0	33.0	40.0	2.0	40.0
42-43	31.051337643669303	37.0	33.0	40.0	2.0	40.0
44-45	30.898971292689122	37.0	27.0	40.0	2.0	40.0
46-47	30.720942073674586	37.0	27.0	40.0	2.0	40.0
48-49	30.576619816514565	37.0	27.0	40.0	2.0	40.0
50-51	30.346383052645415	37.0	27.0	40.0	2.0	40.0
52-53	30.115179784384427	37.0	27.0	38.5	2.0	40.0
54-55	29.951176575348214	37.0	27.0	37.0	2.0	40.0
56-57	29.75208815562155	37.0	27.0	37.0	2.0	40.0
58-59	29.476417432051104	37.0	27.0	37.0	2.0	40.0
60-61	29.27173122193759	37.0	27.0	37.0	2.0	40.0
62-63	29.009037495965337	33.0	27.0	37.0	2.0	40.0
64-65	28.749501620925237	33.0	27.0	37.0	2.0	40.0
66-67	28.50199341686188	33.0	27.0	37.0	2.0	40.0
68-69	28.500160093836865	33.0	27.0	37.0	2.0	40.0
70-71	28.44959532553839	33.0	27.0	37.0	2.0	38.5
72-73	28.282649596752464	33.0	27.0	37.0	2.0	37.0
74-75	28.047034748914196	33.0	27.0	37.0	2.0	37.0
76-77	27.820934272828936	33.0	27.0	37.0	2.0	37.0
78-79	27.58155269543331	33.0	27.0	37.0	2.0	37.0
80-81	27.37102470098747	33.0	24.5	37.0	2.0	37.0
82-83	27.147832488548318	33.0	22.0	37.0	2.0	37.0
84-85	26.967941482620073	33.0	22.0	37.0	2.0	37.0
86-87	26.763931942419518	33.0	22.0	37.0	2.0	37.0
88-89	26.518847618708584	33.0	22.0	37.0	2.0	37.0
90-91	26.297052846478365	33.0	22.0	37.0	2.0	37.0
92-93	26.057871311800945	33.0	22.0	37.0	2.0	37.0
94-95	25.800854503340194	33.0	15.0	37.0	2.0	37.0
96-97	25.454071588991987	33.0	15.0	37.0	2.0	37.0
98-99	25.004473106323587	33.0	2.0	37.0	2.0	37.0
100-101	22.16001744426199	30.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.624855849307508
1101	2	0.608402505815949
1101	3	0.5950783607836918
1101	4	0.6924843554695457
1101	5	0.6656044270839843
1101	6	0.7288943911664347
1101	7	0.7704290209693951
1101	8	0.7561941658829383
1101	9	0.7859471072642137
1101	10-11	0.7726584037447921
1101	12-13	0.7222344935998635
1101	14-15	1.1505459068421047
1101	16-17	1.1895365353845335
1101	18-19	1.1593859986422288
1101	20-21	1.1984465290127204
1101	22-23	1.2479589848035673
1101	24-25	1.3629923860972184
1101	26-27	1.4146074898691765
1101	28-29	1.5237375836163238
1101	30-31	1.392393871393793
1101	32-33	1.548872373452241
1101	34-35	1.5354891521141951
1101	36-37	1.4597950815520342
1101	38-39	1.5406424242904357
1101	40-41	1.5951530723673706
1101	42-43	1.6681665020978045
1101	44-45	1.5863913730057106
1101	46-47	1.575915575937767
1101	48-49	1.5635134344973878
1101	50-51	1.6663495531080876
1101	52-53	1.7007216278601156
1101	54-55	1.6481907350118519
1101	56-57	1.6169968736083788
1101	58-59	1.6479074152350783
1101	60-61	1.5914479525064031
1101	62-63	1.6123058291896797
1101	64-65	1.6775740330739701
1101	66-67	1.6638207129559994
1101	68-69	1.707848834679897
1101	70-71	1.7349733453784744
1101	72-73	1.7606028643116893
1101	74-75	1.7311277784674246
1101	76-77	1.7403962850580683
1101	78-79	1.7817755865444163
1101	80-81	1.8596554306100366
1101	82-83	1.8910094979982084
1101	84-85	1.8707992585429807
1101	86-87	1.859791647196733
1101	88-89	1.8739211792159374
1101	90-91	1.9127920069001014
1101	92-93	1.996071744741208
1101	94-95	1.9878068211474549
1101	96-97	2.06772376697959
1101	98-99	2.155016165618008
1101	100-101	2.1489564292023857
1102	1	0.6502932330309257
1102	2	0.6724472577600586
1102	3	0.6414780323522038
1102	4	0.7325451370438998
1102	5	0.743700205457138
1102	6	0.7350630389508552
1102	7	0.7429077080626669
1102	8	0.7868301655964558
1102	9	0.8007085341104556
1102	10-11	0.7904616690113642
1102	12-13	0.7779542920443632
1102	14-15	1.1797385247517056
1102	16-17	1.1725182291835807
1102	18-19	1.1751332421965373
1102	20-21	1.2002563719839472
1102	22-23	1.1970035617171888
1102	24-25	1.3695872850425417
1102	26-27	1.4501529520708445
1102	28-29	1.554181046412765
1102	30-31	1.450102363694036
1102	32-33	1.5701960063011597
1102	34-35	1.4515868729445245
1102	36-37	1.416268622535089
1102	38-39	1.4308345871657373
1102	40-41	1.5028502962852137
1102	42-43	1.4829817818934146
1102	44-45	1.457580190307155
1102	46-47	1.4162618404465555
1102	48-49	1.4404533740765615
1102	50-51	1.494128852593473
1102	52-53	1.421176364730453
1102	54-55	1.4075021446696496
1102	56-57	1.4009403040093886
1102	58-59	1.3931593483861953
1102	60-61	1.4485811738692647
1102	62-63	1.4927562077208734
1102	64-65	1.5375484078718742
1102	66-67	1.4983191165611451
1102	68-69	1.5489734292316761
1102	70-71	1.5901157582571877
1102	72-73	1.574029071697531
1102	74-75	1.5751554046434038
1102	76-77	1.6255462244617007
1102	78-79	1.6819208374456736
1102	80-81	1.65575721571118
1102	82-83	1.6657266173502414
1102	84-85	1.633680022050175
1102	86-87	1.656781756200047
1102	88-89	1.7920288307327255
1102	90-91	1.8502127121307943
1102	92-93	1.7881100356991553
1102	94-95	1.8245783771852793
1102	96-97	1.8483146092532508
1102	98-99	1.9655000548456414
1102	100-101	1.9327769237683938
1103	1	0.3409226411959452
1103	2	0.3400565506076916
1103	3	0.294972208486854
1103	4	0.3647503789760833
1103	5	0.361056737141233
1103	6	0.360106327971053
1103	7	0.4637569257245673
1103	8	0.4595125272881404
1103	9	0.5003965471966296
1103	10-11	0.5050945294075575
1103	12-13	0.4459212917194364
1103	14-15	0.7083720359196448
1103	16-17	0.7816174516301118
1103	18-19	0.6690268780215547
1103	20-21	0.6636237694181446
1103	22-23	0.6487528544672045
1103	24-25	0.7079953000851376
1103	26-27	0.7261418715517394
1103	28-29	0.7051922132218209
1103	30-31	0.6783841508014348
1103	32-33	0.8740406152632545
1103	34-35	0.8637870229383324
1103	36-37	0.8052560377108726
1103	38-39	0.8646221770693963
1103	40-41	0.8618570842305111
1103	42-43	0.7270477543596456
1103	44-45	0.6668328400722991
1103	46-47	0.5712824451761058
1103	48-49	0.6010998375551253
1103	50-51	0.41300331394726797
1103	52-53	0.45653565337794433
1103	54-55	0.46326108489087403
1103	56-57	0.3837037589813086
1103	58-59	0.394480167661019
1103	60-61	0.33829295316095553
1103	62-63	0.44147924061302746
1103	64-65	0.4278683945418358
1103	66-67	0.47263562886481836
1103	68-69	0.4643860862719613
1103	70-71	0.5696535270701624
1103	72-73	0.2987008480075559
1103	74-75	0.3771443590361372
1103	76-77	0.43948787170876
1103	78-79	0.474473344735042
1103	80-81	0.4473303125335164
1103	82-83	0.46552773944548065
1103	84-85	0.44207877181860766
1103	86-87	0.43723511151413064
1103	88-89	0.32901567418155153
1103	90-91	0.409798538172506
1103	92-93	0.4658801895800124
1103	94-95	0.517219629920298
1103	96-97	0.5358783790741732
1103	98-99	0.4944772114680305
1103	100-101	0.4800633711895159
1104	1	0.30994692334355634
1104	2	0.3364383605841397
1104	3	0.34470085025446906
1104	4	0.41825634016223745
1104	5	0.40439332202974754
1104	6	0.4572902604590823
1104	7	-1.0565472921045043
1104	8	0.15560773251259974
1104	9	0.41535547124127703
1104	10-11	0.4180513110598483
1104	12-13	0.5252942825875522
1104	14-15	0.6896906997827514
1104	16-17	0.7527435010794221
1104	18-19	0.8027693806369456
1104	20-21	0.7983855448540211
1104	22-23	0.856270603887431
1104	24-25	0.8873933573126607
1104	26-27	0.9197947377392737
1104	28-29	0.994109003571964
1104	30-31	0.9142328249278009
1104	32-33	1.0532142715465937
1104	34-35	0.9589183673072696
1104	36-37	0.8990794658330579
1104	38-39	0.9316926526339877
1104	40-41	0.8712722631885619
1104	42-43	0.9444384164009563
1104	44-45	0.7338486530438644
1104	46-47	0.5930306634808282
1104	48-49	0.7172503408215469
1104	50-51	0.5312488444557175
1104	52-53	0.8116058885762101
1104	54-55	0.694723413282432
1104	56-57	0.6993201272790408
1104	58-59	0.7325771795776852
1104	60-61	0.7176747666302923
1104	62-63	0.7321149358298342
1104	64-65	0.7520987421481351
1104	66-67	0.7138851192333568
1104	68-69	0.5808585539593913
1104	70-71	0.6884637499031996
1104	72-73	0.6413475436745877
1104	74-75	0.5872937331718724
1104	76-77	0.6543087025348555
1104	78-79	0.5636342561872887
1104	80-81	0.596736547249332
1104	82-83	0.6270073604190891
1104	84-85	0.6584798967908014
1104	86-87	0.6935218102278604
1104	88-89	0.6793602271201209
1104	90-91	0.6977853536592953
1104	92-93	0.7395290164812849
1104	94-95	0.7311602010380902
1104	96-97	0.758579086713155
1104	98-99	0.6687011011835438
1104	100-101	0.7439420866342132
1105	1	0.19713532294529656
1105	2	0.19583858034198798
1105	3	0.1365084471393594
1105	4	0.24152715023213744
1105	5	0.097654010075793
1105	6	0.2005512482616254
1105	7	-1.6815501240031523
1105	8	-0.39172821702034355
1105	9	0.11841611016439657
1105	10-11	0.21336444310349378
1105	12-13	0.32743250480211117
1105	14-15	0.4346622253894097
1105	16-17	0.5359207612464587
1105	18-19	0.5670271644669214
1105	20-21	0.528301911106162
1105	22-23	0.5250111026455286
1105	24-25	0.669081249935843
1105	26-27	0.5744918900152101
1105	28-29	0.6874365148658512
1105	30-31	0.5728600215501203
1105	32-33	0.6819373914418634
1105	34-35	0.5850402366249412
1105	36-37	0.3729217668993563
1105	38-39	0.29821989991095066
1105	40-41	0.37528893372354943
1105	42-43	0.39195947177706714
1105	44-45	0.2668709092690129
1105	46-47	0.23861038810064983
1105	48-49	0.33248266047780106
1105	50-51	0.333082114821611
1105	52-53	0.39146237613077517
1105	54-55	0.0956510264260011
1105	56-57	0.22432758942417408
1105	58-59	0.20588695313130856
1105	60-61	0.05572214059869296
1105	62-63	0.1302933010642242
1105	64-65	0.12010939243179308
1105	66-67	0.17596154694188115
1105	68-69	-0.011528188775432824
1105	70-71	0.22216926849739949
1105	72-73	0.20427662125351986
1105	74-75	0.17208353540373977
1105	76-77	0.20816257513100567
1105	78-79	0.2155273925765222
1105	80-81	0.2395928116511037
1105	82-83	0.07017315971542004
1105	84-85	0.20284522055913712
1105	86-87	0.26789480508587005
1105	88-89	0.352920777720783
1105	90-91	0.26372338835924936
1105	92-93	0.306598994464494
1105	94-95	0.2097024284527862
1105	96-97	0.23760437389559286
1105	98-99	0.23888016872770734
1105	100-101	0.329636997272317
1106	1	-0.3512205164308355
1106	2	-0.3251605334842864
1106	3	-0.39592520095401085
1106	4	-0.37532025179214656
1106	5	-0.5832388245942397
1106	6	-0.47256847428946713
1106	7	-0.23860883533904342
1106	8	-0.3169949510848724
1106	9	0.1308656704513922
1106	10-11	-0.13090077060942207
1106	12-13	-0.40181203337815674
1106	14-15	-0.5942867019823694
1106	16-17	-0.4860633332352364
1106	18-19	-0.6415843022665939
1106	20-21	-0.4549053661247058
1106	22-23	-0.3964817679922916
1106	24-25	-0.25000826467696413
1106	26-27	-0.38757141918974725
1106	28-29	-0.5119813487654028
1106	30-31	-0.7597917466711763
1106	32-33	-1.0162267138183871
1106	34-35	-0.6040096705085034
1106	36-37	-0.7743582305091685
1106	38-39	-0.7167695588405962
1106	40-41	-0.785543676358138
1106	42-43	-0.489748040175666
1106	44-45	-0.6490157515939785
1106	46-47	-0.849158550243235
1106	48-49	-1.0155340031648628
1106	50-51	-0.7390940604917269
1106	52-53	-1.2993589554458005
1106	54-55	-1.2886047999426964
1106	56-57	-0.8918640971404379
1106	58-59	-0.7288673395557801
1106	60-61	-0.7948986881316991
1106	62-63	-0.5895863181603929
1106	64-65	-0.9542500044129731
1106	66-67	-0.976862763529482
1106	68-69	-0.8542609699916675
1106	70-71	-0.8698585244742922
1106	72-73	-1.155290885375564
1106	74-75	-1.2979513462631402
1106	76-77	-0.9840147106146446
1106	78-79	-1.123304411205563
1106	80-81	-1.3110172812520595
1106	82-83	-1.203185927987203
1106	84-85	-1.0580799532150742
1106	86-87	-1.057754427701294
1106	88-89	-1.611012982425354
1106	90-91	-1.1442929598839058
1106	92-93	-1.2866880254512338
1106	94-95	-1.0934629111214953
1106	96-97	-1.0256103513080532
1106	98-99	-1.2833864955757193
1106	100-101	-1.063241422521756
1107	1	0.16272157674654153
1107	2	0.17249984476698188
1107	3	0.2196014816484535
1107	4	0.2475462426732733
1107	5	0.23599048893566277
1107	6	0.24048804146029568
1107	7	0.35463226301986595
1107	8	0.33431159557606094
1107	9	0.25004607815206015
1107	10-11	0.27549926014683024
1107	12-13	0.30003577553422645
1107	14-15	0.3956151793598792
1107	16-17	0.4314064181826751
1107	18-19	0.3681362326331694
1107	20-21	0.35418638356555476
1107	22-23	0.35491168199512657
1107	24-25	0.44010458431290544
1107	26-27	0.43612008090165233
1107	28-29	0.5131834802712092
1107	30-31	0.37310526464865035
1107	32-33	0.5805445551053694
1107	34-35	0.444033237287222
1107	36-37	0.33324919740866576
1107	38-39	0.3324448441007384
1107	40-41	0.3927713341882715
1107	42-43	0.38668029084390554
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2216	100-101	-1.4306331386540592
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	611829.0
3	164523.0
4	143745.0
5	156576.0
6	199318.0
7	186981.0
8	140027.0
9	142419.0
10	141014.0
11	139741.0
12	135853.0
13	143317.0
14	159808.0
15	168390.0
16	130886.0
17	123004.0
18	124535.0
19	129733.0
20	143668.0
21	162165.0
22	185429.0
23	217133.0
24	253436.0
25	297273.0
26	343021.0
27	396784.0
28	458363.0
29	532998.0
30	622618.0
31	734927.0
32	878043.0
33	1067547.0
34	1323046.0
35	1685591.0
36	2294277.0
37	3333729.0
38	2039160.0
39	2297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.155661508190327	12.6150063017833	19.599481134937744	39.62985105508863
2	22.296782366855705	23.94841374345027	31.258119631083954	22.496684258610074
3	25.391083377572578	24.804534157023806	27.403314994065127	22.40106747133849
4	27.420816496057054	27.880987717908535	19.155221446831757	25.54297433920265
5	28.762579830791797	30.108775282133866	19.898520833219358	21.23012405385498
6	23.964584973097505	33.610372039509706	19.456287438967948	22.968755548424838
7	21.738937980474883	16.935255970608377	38.28001617394652	23.04578987497022
8	20.709925927694922	25.058330447657827	24.38905274218778	29.842690882459472
9	22.146122332533157	22.665773780747166	27.632665578810112	27.55543830790956
10-11	27.236042254387684	29.752923532533813	19.114944191797324	23.89609002128118
12-13	25.021857890337802	21.92225037239737	26.294330805852596	26.76156093141223
14-15	24.187625739139847	23.72685351560256	26.063304159108906	26.022216586148684
16-17	25.021274297134948	24.93713957204796	25.98668980193373	24.054896328883363
18-19	25.287056861961275	25.277989539303906	24.927584521448544	24.507369077286267
20-21	25.927319342451195	26.32451822754919	24.34620744625466	23.40195498374495
22-23	27.68343254225093	24.92510804200918	24.211417619244124	23.180041796495765
24-25	24.698809608576283	26.932312179471285	25.062664190001627	23.3062140219508
26-27	24.352572043165104	26.533563158995154	24.019718769212602	25.09414602862714
28-29	24.681336356911245	26.984442076486243	24.8279620612867	23.506259505315807
30-31	26.13495830340648	25.83454495122503	24.756249634174342	23.274247111194143
32-33	24.85140295418052	25.903510916867063	26.007210312002886	23.23787581694953
34-35	24.913165022531363	26.843928442876663	24.092144712510493	24.15076182208148
36-37	24.21301723871744	24.717601082113745	26.511512897413347	24.557868781755467
38-39	24.18715665229817	25.23794771747319	25.755474355445763	24.819421274782872
40-41	27.41332039548793	24.597563400235835	24.47059161882707	23.518524585449175
42-43	25.60187407094568	24.624579421085045	25.71469734944738	24.058849158521898
44-45	26.807228900590985	25.22049341708416	24.62604523619582	23.346232446129033
46-47	25.05871734603975	25.078445103487297	24.358336927532612	25.50450062294034
48-49	24.60094330756881	24.526872274068026	24.67402629371606	26.198158124647104
50-51	26.118017289840274	24.658375650287525	25.516454273551716	23.70715278632049
52-53	24.399277917254967	25.704483203876453	25.53599239373622	24.360246485132357
54-55	23.264876771834718	25.53804447599165	25.529538338388065	25.667540413785566
56-57	23.255032844370213	24.55751086159222	28.81774200605049	23.36971428798708
58-59	23.429177037348868	26.74078544419168	24.762836198297226	25.06720132016223
60-61	23.809327248430893	28.548292258640778	24.29328353175711	23.349096961171224
62-63	23.19776301990212	28.275676018765054	24.728785527329002	23.797775434003825
64-65	23.47419570475467	28.344989097269963	24.65440997667346	23.526405221301903
66-67	23.43394244921771	28.568052259096227	24.51923869549999	23.478766596186073
68-69	24.522507179378536	26.92042574071898	24.570657863094976	23.986409216807512
70-71	24.95617322651852	26.53617398066205	24.681966739267786	23.82568605355165
72-73	24.633572314420483	26.699811932325662	24.905944893303786	23.760670859950064
74-75	25.003703043512633	26.52351618439598	24.833379308046	23.639401464045392
76-77	25.16667238112447	25.515786544228774	24.881922200117977	24.435618874528775
78-79	24.63401345903104	25.471606649932554	25.36851918124645	24.525860709789956
80-81	25.290193764327434	25.69693465400096	25.20577735485457	23.80709422681704
82-83	25.22141286088846	25.69024858434065	25.13208591103804	23.95625264373285
84-85	25.268918738984375	26.053582081107646	24.953033482150623	23.72446569775736
86-87	25.90475690942688	25.63702649474578	24.852595998813147	23.6056205970142
88-89	24.9583290600004	26.340704088099283	24.743969169736083	23.95699768216424
90-91	25.270259355320214	25.944445074356913	24.694660360500322	24.09063520982255
92-93	25.19581249552944	26.420082017576107	24.793580790946482	23.59052469594797
94-95	25.629036747253135	25.90745153488152	24.84102409009703	23.62248762776831
96-97	25.057493466313126	26.07544647392135	25.283860968158667	23.58319909160686
98-99	25.56811239538203	26.23809286644414	24.560363639184914	23.633431098988915
100-101	25.97670300003679	25.582268594670087	24.347657224594982	24.093371180698146
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5929.0
1	3601.5
2	1092.5
3	901.0
4	878.5
5	881.5
6	953.5
7	1156.5
8	1549.5
9	2149.5
10	2894.5
11	3803.0
12	4805.0
13	5974.0
14	7238.5
15	8473.5
16	9786.0
17	11116.0
18	12734.0
19	14934.5
20	17764.0
21	21834.0
22	27917.5
23	36651.5
24	47979.0
25	62145.5
26	80542.0
27	101659.0
28	123348.0
29	146250.0
30	170734.5
31	197170.5
32	224534.0
33	251416.5
34	279262.5
35	311613.0
36	346927.5
37	383008.5
38	416938.0
39	448806.5
40	477682.0
41	503776.5
42	533014.5
43	566279.5
44	590414.0
45	610121.5
46	632358.5
47	646231.5
48	663013.5
49	678321.0
50	694810.0
51	712065.5
52	714469.5
53	706895.5
54	685505.5
55	661686.0
56	634325.5
57	597798.0
58	567703.5
59	539898.0
60	510079.0
61	477507.0
62	441127.5
63	406387.5
64	372309.5
65	333325.0
66	287752.5
67	248513.5
68	213207.5
69	172411.5
70	132488.0
71	97517.5
72	70038.5
73	48432.0
74	32431.5
75	21008.0
76	13170.0
77	8259.5
78	5273.0
79	3336.0
80	2215.5
81	1542.5
82	1111.0
83	822.5
84	583.5
85	433.0
86	332.0
87	232.5
88	150.5
89	100.0
90	74.0
91	57.0
92	40.0
93	32.0
94	21.0
95	15.5
96	15.0
97	13.5
98	14.0
99	19.0
100	28.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038700944911611294
2	0.03401248254629148
3	0.03292364558128084
4	0.03368433989930197
5	0.03288387071497907
6	0.03055704103632619
7	0.033778805206768646
8	0.06922815479821116
9	0.037845785286123486
10-11	0.038019800326193676
12-13	0.041445410686432656
14-15	0.0426834034000749
16-17	0.04248204313942224
18-19	0.039978712491555296
20-21	0.059985470241339975
22-23	0.04180587041229235
24-25	0.04483870396780145
26-27	0.041761123687702864
28-29	0.14115105678836648
30-31	0.15019238108458505
32-33	0.14513351527683008
34-35	0.11262999172086158
36-37	0.16896363204987133
38-39	0.3119269311841117
40-41	0.5975129571598836
42-43	0.6472489415410891
44-45	0.6382225328197337
46-47	0.6271377747672623
48-49	0.6179199495018298
50-51	0.6154017032790997
52-53	0.6238041437853461
54-55	0.6345831325531228
56-57	0.6226133837254373
58-59	0.6322985636699155
60-61	0.6298747827546521
62-63	0.6357390896050177
64-65	0.6333351961229051
66-67	0.6330393705547858
68-69	0.6468511928780716
70-71	0.6476864650704085
72-73	0.6541324793404373
74-75	0.6613441597867749
76-77	0.6694060280003127
78-79	0.6664974909019965
80-81	0.6675639545047124
82-83	0.6679741328134493
84-85	0.6573517575817358
86-87	0.6595542908031957
88-89	0.6694756340163408
90-91	0.6805653639270998
92-93	0.6726476796039059
94-95	0.67169059688352
96-97	0.6693587953465794
98-99	0.6719913943099269
100-101	0.6610035874940661
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.0113204E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.06963795282029
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.34127185107447	45.27977659257111
2	11.27496889620228	12.869167856711474
3	3.6968338509342864	6.329309083336502
4	1.698162590587558	3.8765409691942114
5	0.9565727696464212	2.7295630819623904
6	0.6236955407740428	2.1356447222857846
7	0.4115947241931524	1.644269332509589
8	0.3075913923140213	1.4043303517412082
9	0.22549866334357266	1.1582214368276338
>10	1.3231785005849297	14.03783660424241
>50	0.0990619213114276	3.8760384423038445
>100	0.04056699669086721	3.877430907566448
>500	7.571571369958025E-4	0.2776474435478341
>1k	2.1890662303488157E-4	0.2790501709554824
>5k	8.746194341560896E-6	0.03240929944484492
>10k+	1.7492388683121754E-5	0.19276370479908564
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTG	24029	0.11946878279562023	TruSeq Adapter, Index 3 (100% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.10561718560603274	1.4915574863159544E-5	0.0	0.0	0.0
2	0.105985103119324	3.9774866301758785E-5	0.0	1.9887433150879393E-5	0.0
3	0.10604973727706436	3.9774866301758785E-5	0.0	1.9887433150879393E-5	0.0
4	0.10627844275829948	3.9774866301758785E-5	0.0	2.485929143859924E-5	0.0
5	0.10635302063261527	3.9774866301758785E-5	0.0	2.485929143859924E-5	4.971858287719848E-6
6	0.10665630398816618	4.4746724589478636E-5	0.0	2.9831149726319087E-5	4.971858287719848E-6
7	0.10678060044535918	5.9662299452638175E-5	0.0	2.9831149726319087E-5	4.971858287719848E-6
8	0.10714354610036272	5.9662299452638175E-5	0.0	2.9831149726319087E-5	4.971858287719848E-6
9	0.10757609777139436	5.9662299452638175E-5	0.0	3.9774866301758785E-5	4.971858287719848E-6
10-11	0.1081752066950646	5.9662299452638175E-5	0.0	3.9774866301758785E-5	4.971858287719848E-6
12-13	0.10886380906791379	6.960601602807787E-5	2.485929143859924E-6	4.4746724589478636E-5	4.971858287719848E-6
14-15	0.11014654850614553	8.203566174737749E-5	4.971858287719848E-6	4.4746724589478636E-5	4.971858287719848E-6
16-17	0.11126273069173861	8.452159089123741E-5	4.971858287719848E-6	5.469044116491833E-5	4.971858287719848E-6
18-19	0.11272197109918439	8.452159089123741E-5	4.971858287719848E-6	6.463415774035803E-5	4.971858287719848E-6
20-21	0.13092643021967063	9.695123661053703E-5	9.943716575439696E-6	6.463415774035803E-5	7.457787431579773E-6
22-23	0.13221911337447778	1.0440902404211682E-4	9.943716575439696E-6	6.712008688421794E-5	1.4915574863159544E-5
24-25	0.13326320361489896	1.2678238633685612E-4	9.943716575439696E-6	7.457787431579773E-5	1.4915574863159544E-5
26-27	0.134468879249671	1.342401737684359E-4	1.242964571929962E-5	7.954973260351757E-5	1.9887433150879393E-5
28-29	0.135622350372422	1.367261029122958E-4	1.9887433150879393E-5	7.954973260351757E-5	2.485929143859924E-5
30-31	0.13713130936274498	1.7650096921405462E-4	1.9887433150879393E-5	8.203566174737749E-5	2.485929143859924E-5
32-33	0.13845382366727849	2.0384618979651378E-4	1.9887433150879393E-5	8.949344917895727E-5	2.485929143859924E-5
34-35	0.13965701337290667	2.0881804808423364E-4	1.9887433150879393E-5	9.446530746667712E-5	2.485929143859924E-5
36-37	0.14099941511059103	2.2621955209125308E-4	1.9887433150879393E-5	9.446530746667712E-5	2.485929143859924E-5
38-39	0.14440016617939141	2.3367733952283287E-4	1.9887433150879393E-5	9.943716575439696E-5	2.485929143859924E-5
40-41	0.1478183187521988	2.4362105609827253E-4	1.9887433150879393E-5	1.0440902404211682E-4	2.485929143859924E-5
42-43	0.14967033596437446	2.485929143859924E-4	1.9887433150879393E-5	1.1683866976141643E-4	2.485929143859924E-5
44-45	0.15205185608419225	2.6102256010529203E-4	1.9887433150879393E-5	1.2678238633685612E-4	2.485929143859924E-5
46-47	0.15437619983370127	2.6350848924915196E-4	1.9887433150879393E-5	1.2926831548071605E-4	2.485929143859924E-5
48-49	0.15670551544149802	2.809099932561714E-4	1.9887433150879393E-5	1.3175424462457598E-4	2.485929143859924E-5
50-51	0.159226247593372	2.883677806877512E-4	1.9887433150879393E-5	1.3921203205615574E-4	2.485929143859924E-5
52-53	0.16202540380935826	2.95825568119331E-4	1.9887433150879393E-5	1.566135360631752E-4	2.485929143859924E-5
54-55	0.16515270267233406	3.0079742640705084E-4	1.9887433150879393E-5	1.64071323494755E-4	2.485929143859924E-5
56-57	0.1686479190486011	3.132270721263504E-4	1.9887433150879393E-5	1.6904318178247483E-4	2.485929143859924E-5
58-59	0.17291128753032087	3.2317078870179014E-4	1.9887433150879393E-5	1.740150400701947E-4	2.485929143859924E-5
60-61	0.1784126487256829	3.2565671784565007E-4	1.9887433150879393E-5	1.9141654407721416E-4	2.485929143859924E-5
62-63	0.18842099945886293	3.3560043442108973E-4	1.9887433150879393E-5	2.088180480842336E-4	2.9831149726319087E-5
64-65	0.22465838858890907	3.5300193842810923E-4	1.9887433150879393E-5	2.1130397722809354E-4	2.9831149726319087E-5
66-67	0.23853981692822288	3.579737967158291E-4	1.9887433150879393E-5	2.1378990637195347E-4	2.9831149726319087E-5
68-69	0.24654202284230797	3.579737967158291E-4	1.9887433150879393E-5	2.1876176465967332E-4	2.9831149726319087E-5
70-71	0.25713456692429515	3.6791751329126875E-4	1.9887433150879393E-5	2.2373362294739315E-4	2.9831149726319087E-5
72-73	0.274048828819118	3.728893715789886E-4	1.9887433150879393E-5	2.485929143859924E-4	2.9831149726319087E-5
74-75	0.2983288987671979	3.8531901729828825E-4	1.9887433150879393E-5	2.560507018175722E-4	2.9831149726319087E-5
76-77	0.3250327496305412	3.878049464421482E-4	1.9887433150879393E-5	2.734522058245916E-4	2.9831149726319087E-5
78-79	0.353715400092397	3.9029087558600805E-4	1.9887433150879393E-5	2.809099932561714E-4	2.9831149726319087E-5
80-81	0.3947058857455033	4.0769237959302756E-4	1.9887433150879393E-5	3.032833555509107E-4	2.9831149726319087E-5
82-83	0.467208009226178	4.226079544561871E-4	1.9887433150879393E-5	3.032833555509107E-4	2.9831149726319087E-5
84-85	0.5804594832330046	4.400094584632066E-4	1.9887433150879393E-5	3.132270721263504E-4	3.4803008014038935E-5
86-87	0.7117215138870963	4.424953876070665E-4	2.485929143859924E-5	3.206848595579302E-4	3.4803008014038935E-5
88-89	0.8417306362526826	4.5243910418250616E-4	2.485929143859924E-5	3.3560043442108973E-4	3.4803008014038935E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACGGC	30610	0.0	73.13143	9
AAGACGG	31820	0.0	69.80802	8
GAAGACG	34735	0.0	65.542305	7
AGAAGAC	41840	0.0	45.50366	6
ATCGGAA	61390	0.0	43.20007	2
AACCGGA	6930	0.0	42.99905	2
TCGGAAG	57025	0.0	42.832832	3
GATCGGA	63485	0.0	42.283905	1
ATCGGGA	7450	0.0	41.584988	2
CATACGA	22130	0.0	41.407566	14-15
AGCAGAA	50175	0.0	41.101246	3
AAGCAGA	50460	0.0	40.653103	2
GATCGGG	7915	0.0	40.564503	1
GACGCGT	7880	0.0	40.271023	44-45
ATACGAG	21025	0.0	40.242455	16-17
GCATACG	23170	0.0	40.22274	14-15
CAAGCAG	39190	0.0	39.97362	1
CGGCATA	23925	0.0	39.163727	12-13
ACGCGTG	7910	0.0	38.6117	46-47
GCAGAAG	52680	0.0	37.943974	4
>>END_MODULE