FastQCFastQC Report
Wed 25 May 2016
SRR1033293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20113204
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC6933283.447128562908227TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG205690.10226615312010955TruSeq Adapter, Index 3 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG1066900.066.198623
CGGAAGA1084100.065.1705554
ATCGGAA1096250.064.7561952
GATCGGA1117000.063.6729741
AGAGCAC1125150.063.0845878
GAGCACA1127950.063.054239
AAGAGCA1203600.058.9445767
GAAGAGC1229150.057.758396
GGAAGAG1334300.053.227675
CGTATGC813850.044.4584844-45
CTCGTAT801400.044.45533842-43
TATGCCG818300.044.22677646-47
ATCTCGT817000.043.40929840-41
TCGTATG808900.043.12337542-43
ACTTAGG857900.042.5807832-33
TTAGGCA862600.042.41005334-35
TCTCGTA812500.042.10379440-41
CCGTCTT857450.041.9542750-51
TGCCGTC855500.041.951648-49
ATGCCGT842450.041.19030446-47
Produced by FastQC (version 0.11.5)