##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033293_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20113204
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.500445776814075	33.0	33.0	33.0	33.0	33.0
2	32.59318276690278	33.0	33.0	33.0	33.0	33.0
3	32.546246237049054	33.0	33.0	33.0	33.0	33.0
4	36.32043537170905	37.0	37.0	37.0	37.0	37.0
5	36.43097643717033	37.0	37.0	37.0	37.0	37.0
6	36.303498786170515	37.0	37.0	37.0	37.0	37.0
7	36.26300334844712	37.0	37.0	37.0	33.0	37.0
8	36.28730131708504	37.0	37.0	37.0	37.0	37.0
9	36.29636228022149	37.0	37.0	37.0	37.0	37.0
10-11	36.30766793793769	37.0	37.0	37.0	37.0	37.0
12-13	36.25828639733381	37.0	37.0	37.0	35.0	37.0
14-15	38.052644397183066	40.0	37.0	40.0	33.0	40.0
16-17	38.00450614432191	40.0	37.0	40.0	33.0	40.0
18-19	37.9365253790495	40.0	37.0	40.0	33.0	40.0
20-21	37.98161667827762	40.0	37.0	40.0	33.0	40.0
22-23	37.92683972677849	40.0	37.0	40.0	33.0	40.0
24-25	37.24353844867282	40.0	37.0	40.0	33.0	40.0
26-27	36.20314930927961	37.0	37.0	40.0	30.0	40.0
28-29	35.99268836034278	37.0	37.0	40.0	27.0	40.0
30-31	35.777417511401964	37.0	37.0	40.0	27.0	40.0
32-33	35.67054846159766	37.0	37.0	40.0	27.0	40.0
34-35	35.750901547063314	37.0	37.0	40.0	27.0	40.0
36-37	35.77902916412522	37.0	37.0	40.0	27.0	40.0
38-39	35.69010191016807	37.0	37.0	40.0	27.0	40.0
40-41	35.52283788301456	37.0	37.0	40.0	27.0	40.0
42-43	35.24993653422896	37.0	33.0	40.0	27.0	40.0
44-45	35.092241096943084	37.0	33.0	40.0	27.0	40.0
46-47	34.96430966443735	37.0	33.0	40.0	27.0	40.0
48-49	34.84750393323709	37.0	33.0	40.0	27.0	40.0
50-51	34.75129815219893	37.0	33.0	40.0	27.0	40.0
52-53	34.57211635699613	37.0	33.0	40.0	24.5	40.0
54-55	34.41598710478947	37.0	33.0	40.0	24.5	40.0
56-57	34.270469240007706	37.0	33.0	40.0	24.5	40.0
58-59	34.05829058861035	37.0	33.0	40.0	22.0	40.0
60-61	33.825629869810896	37.0	33.0	40.0	22.0	40.0
62-63	33.63010043054304	37.0	33.0	38.5	22.0	40.0
64-65	33.14334911533737	37.0	33.0	37.0	22.0	40.0
66-67	32.87787848221497	37.0	33.0	37.0	22.0	40.0
68-69	32.46089954141568	37.0	33.0	37.0	22.0	40.0
70-71	32.13618073977672	37.0	33.0	37.0	22.0	40.0
72-73	31.744166568389602	37.0	33.0	37.0	18.5	37.0
74-75	31.153837772440433	37.0	33.0	37.0	15.0	37.0
76-77	30.960829637088153	37.0	33.0	37.0	15.0	37.0
78-79	30.751034096805263	33.0	33.0	37.0	15.0	37.0
80-81	30.531459184722635	33.0	27.0	37.0	15.0	37.0
82-83	30.319316181549198	33.0	27.0	37.0	6.0	37.0
84-85	30.16624198710459	33.0	27.0	37.0	6.0	37.0
86-87	29.985439266662837	33.0	27.0	37.0	6.0	37.0
88-89	29.764131736544808	33.0	27.0	37.0	2.0	37.0
90-91	29.535773887641174	33.0	27.0	37.0	2.0	37.0
92-93	29.276804978460916	33.0	27.0	37.0	2.0	37.0
94-95	29.026039958626185	33.0	27.0	37.0	2.0	37.0
96-97	28.882109036431988	33.0	27.0	37.0	2.0	37.0
98-99	28.590134147697206	33.0	27.0	37.0	2.0	37.0
100-101	26.186891630990267	30.0	21.0	35.0	2.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17017784904054878
1101	2	0.13320107150111227
1101	3	0.2092779881291662
1101	4	0.32948377455122824
1101	5	0.2237408504074594
1101	6	0.26490877459816886
1101	7	0.2806173981165827
1101	8	0.2838817549195909
1101	9	0.2769380920382716
1101	10-11	0.2769732814808634
1101	12-13	0.2904053080888218
1101	14-15	0.681607309606477
1101	16-17	0.714722665041613
1101	18-19	0.7121916301252185
1101	20-21	0.6783561067175157
1101	22-23	0.6528618630033804
1101	24-25	0.7916689588267403
1101	26-27	0.915370121450259
1101	28-29	0.9697508158071955
1101	30-31	1.0580894840109352
1101	32-33	1.1202165838654068
1101	34-35	1.0314957941609038
1101	36-37	1.010737588186828
1101	38-39	0.9950263379391657
1101	40-41	0.9834601221626968
1101	42-43	1.0874975800055395
1101	44-45	1.1479433033961683
1101	46-47	1.1404179098823448
1101	48-49	1.0839931497384683
1101	50-51	1.0764660077162702
1101	52-53	1.003491885743479
1101	54-55	1.1167340198108775
1101	56-57	1.0999544795761977
1101	58-59	1.0845650252915036
1101	60-61	1.1308970720429699
1101	62-63	1.0808639949028347
1101	64-65	1.1043148670986085
1101	66-67	1.121627123420616
1101	68-69	1.1513712181087215
1101	70-71	1.1847547764510935
1101	72-73	1.219628675082852
1101	74-75	1.133523217856947
1101	76-77	1.1391503204006348
1101	78-79	1.1588708536666168
1101	80-81	1.2321132145411298
1101	82-83	1.2458132147922285
1101	84-85	1.1992436707877765
1101	86-87	1.2965127967359784
1101	88-89	1.352858455329443
1101	90-91	1.3527836620994798
1101	92-93	1.4601141863931986
1101	94-95	1.4582656761233928
1101	96-97	1.5526247983393802
1101	98-99	1.6221462486007745
1101	100-101	1.7603109330245807
1102	1	0.1500965705079551
1102	2	0.13520843988782616
1102	3	0.21033323269973891
1102	4	0.30790300626159706
1102	5	0.19481331860396978
1102	6	0.22450148191335018
1102	7	0.2189906506514916
1102	8	0.22157856482018445
1102	9	0.20538313829995758
1102	10-11	0.24482201047097618
1102	12-13	0.2627523629377322
1102	14-15	0.6045889951494772
1102	16-17	0.6535839102630945
1102	18-19	0.6439610572833772
1102	20-21	0.6052293794864951
1102	22-23	0.5864650391194743
1102	24-25	0.7572275182494508
1102	26-27	0.864187178968983
1102	28-29	0.9100987280389532
1102	30-31	0.9178854049795646
1102	32-33	1.0388608015333531
1102	34-35	0.9387159674694203
1102	36-37	0.9042848756187709
1102	38-39	0.9084186414201625
1102	40-41	0.9310341409228613
1102	42-43	0.9889696770010801
1102	44-45	1.1079955751433843
1102	46-47	0.9739579503927303
1102	48-49	0.9647896518857166
1102	50-51	1.0092234921887666
1102	52-53	0.9832790171466286
1102	54-55	0.9803263275467771
1102	56-57	0.9823527649427959
1102	58-59	0.9721594290245861
1102	60-61	1.0041769683338515
1102	62-63	0.981529964614964
1102	64-65	1.0013061947127824
1102	66-67	1.0381491286949327
1102	68-69	1.079769114905595
1102	70-71	1.0753787335132685
1102	72-73	1.0910496971433687
1102	74-75	1.0363581124418104
1102	76-77	1.0756863055958767
1102	78-79	1.0952552723078988
1102	80-81	1.0671216327756134
1102	82-83	1.1570551560136408
1102	84-85	1.1544441734088586
1102	86-87	1.2056919679934417
1102	88-89	1.303996453633392
1102	90-91	1.271945916487109
1102	92-93	1.283050558823696
1102	94-95	1.3714202931210728
1102	96-97	1.3854385730954561
1102	98-99	1.423159246944934
1102	100-101	1.5552947517642295
1103	1	0.1343467634210782
1103	2	0.10432423286955128
1103	3	0.11820233092466736
1103	4	0.2750651788863365
1103	5	0.1698741637783101
1103	6	0.17325335819338505
1103	7	0.1962908246847448
1103	8	0.19394011455748483
1103	9	0.21467772853847578
1103	10-11	0.24401787760579907
1103	12-13	0.2145039393809327
1103	14-15	0.5161713496853295
1103	16-17	0.5171276100275932
1103	18-19	0.4919624533184077
1103	20-21	0.47008989625114594
1103	22-23	0.4830916038794868
1103	24-25	0.6067670646568644
1103	26-27	0.839294674913738
1103	28-29	0.8996563578203549
1103	30-31	0.8962064961289968
1103	32-33	0.956842187202362
1103	34-35	0.8613766122132063
1103	36-37	0.7698521240191312
1103	38-39	0.801949111582978
1103	40-41	0.8149222319785068
1103	42-43	0.8857541038502177
1103	44-45	0.9843260615083622
1103	46-47	0.9323318540112098
1103	48-49	0.8903040003880136
1103	50-51	0.8487585872985051
1103	52-53	0.878713331900471
1103	54-55	0.8878425636282969
1103	56-57	0.8457716049179425
1103	58-59	0.8471755962825966
1103	60-61	0.8458212854430514
1103	62-63	0.8152825327610529
1103	64-65	0.7706640513108596
1103	66-67	0.7334707630611419
1103	68-69	0.7093282887203998
1103	70-71	0.7594412679530436
1103	72-73	0.6480864190413271
1103	74-75	0.6185799322927181
1103	76-77	0.607420451626421
1103	78-79	0.5675780916938926
1103	80-81	0.6506150529447261
1103	82-83	0.5786860294871907
1103	84-85	0.5700634770064887
1103	86-87	0.7508911198583554
1103	88-89	0.6372831328753783
1103	90-91	0.6548559697269702
1103	92-93	0.6730195286806016
1103	94-95	0.6783451292746925
1103	96-97	0.659771928494159
1103	98-99	0.7540318350238593
1103	100-101	0.9308333871717913
1104	1	0.1073331583921302
1104	2	0.056783941866463294
1104	3	-0.008364033297532103
1104	4	0.2082215845814872
1104	5	0.1208368407119238
1104	6	0.1286336585646808
1104	7	0.10138950154951942
1104	8	0.008297808458621603
1104	9	0.06300317926730514
1104	10-11	0.11345542815274001
1104	12-13	0.11991706221014908
1104	14-15	0.31306008701990606
1104	16-17	0.25820320289879106
1104	18-19	0.29760679532098777
1104	20-21	0.37781237593199535
1104	22-23	0.39720861580757116
1104	24-25	0.3607319769056261
1104	26-27	0.4788619397312601
1104	28-29	0.5819751119676013
1104	30-31	0.5968969246212268
1104	32-33	0.5718453152859908
1104	34-35	0.5958678000626207
1104	36-37	0.5094928164022576
1104	38-39	0.5706887079248801
1104	40-41	0.5816460755261801
1104	42-43	0.6045775917539515
1104	44-45	0.6240317243066116
1104	46-47	0.6540536401338102
1104	48-49	0.6009147808349127
1104	50-51	0.6992996442276649
1104	52-53	0.5238943671820095
1104	54-55	0.6387386097865999
1104	56-57	0.608687496160293
1104	58-59	0.6187290034450967
1104	60-61	0.4860097340810938
1104	62-63	0.5127002590691632
1104	64-65	0.5772825125166818
1104	66-67	0.5293910047802868
1104	68-69	0.6778022805353601
1104	70-71	0.7288822793977658
1104	72-73	0.7169385181437704
1104	74-75	0.6877955180139494
1104	76-77	0.6305298272070736
1104	78-79	0.6495690820333415
1104	80-81	0.6156807563887519
1104	82-83	0.6357373412167604
1104	84-85	0.6919296135373649
1104	86-87	0.6761231498343427
1104	88-89	0.7488146783649263
1104	90-91	0.772181867166573
1104	92-93	0.7515819313111081
1104	94-95	0.8011822219468065
1104	96-97	0.8818446116550476
1104	98-99	0.882094065363134
1104	100-101	0.9055085406207866
1105	1	0.08060837827522249
1105	2	0.02191248026981185
1105	3	0.004626295947900871
1105	4	0.2028323297145107
1105	5	0.12944167163104936
1105	6	0.05360241109314501
1105	7	0.0026470336856334598
1105	8	0.06283604586346314
1105	9	0.08794755760926165
1105	10-11	0.13812653476229997
1105	12-13	0.08790498104068689
1105	14-15	0.13692942566416377
1105	16-17	0.11273351027722356
1105	18-19	0.12937016337647833
1105	20-21	0.30960121995806844
1105	22-23	0.36324479746349425
1105	24-25	0.3777657187686714
1105	26-27	0.4258482119092335
1105	28-29	0.4668893293747729
1105	30-31	0.42289419575206466
1105	32-33	0.5574823274113356
1105	34-35	0.4784611618706123
1105	36-37	0.425647237331674
1105	38-39	0.44334857615768186
1105	40-41	0.2690978495964629
1105	42-43	0.48059674052079515
1105	44-45	0.5915067825357809
1105	46-47	0.4351806018539932
1105	48-49	0.45179629872642835
1105	50-51	0.45411953354506096
1105	52-53	0.5298453997217933
1105	54-55	0.46688351418608676
1105	56-57	0.5183732711116562
1105	58-59	0.45624868959991005
1105	60-61	0.4635788186877505
1105	62-63	0.43741305603431613
1105	64-65	0.3691431062644739
1105	66-67	0.47271086373361726
1105	68-69	0.5611137976371552
1105	70-71	0.4855473888056139
1105	72-73	0.47207818051271033
1105	74-75	0.40833891787593046
1105	76-77	0.37291894313716156
1105	78-79	0.5503234165771538
1105	80-81	0.5191951512725375
1105	82-83	0.5242991954113201
1105	84-85	0.5765862102494062
1105	86-87	0.5912726648013837
1105	88-89	0.6483852265547192
1105	90-91	0.6813972259889383
1105	92-93	0.6572191638588265
1105	94-95	0.492294087422362
1105	96-97	0.5395985619063275
1105	98-99	0.5830436104998711
1105	100-101	0.7308315136699939
1106	1	7.548467929012759E-4
1106	2	-0.03166091286314554
1106	3	-0.08210604111894781
1106	4	0.11626630437410768
1106	5	-0.18484203333459703
1106	6	-0.09556966666963262
1106	7	0.013241741120594952
1106	8	-0.07240039836957379
1106	9	-0.0339272272201967
1106	10-11	-0.1934330881976578
1106	12-13	0.008518620510663766
1106	14-15	-0.2693157447808687
1106	16-17	-0.15397867818843025
1106	18-19	0.025518011057684475
1106	20-21	0.08905959670912011
1106	22-23	0.0711263474416981
1106	24-25	-1.3639804765830377E-4
1106	26-27	0.002294662279375359
1106	28-29	-0.229109419340368
1106	30-31	-0.24521769605556898
1106	32-33	-0.11917348689470941
1106	34-35	-0.20689854923756457
1106	36-37	0.044710958410242085
1106	38-39	-0.10249790340839127
1106	40-41	-0.5564890591176805
1106	42-43	0.0435871047126426
1106	44-45	0.266559620341674
1106	46-47	0.2020294575533157
1106	48-49	-0.10681120020121426
1106	50-51	-0.2733262791211786
1106	52-53	0.013005660174911782
1106	54-55	-0.07607463169287598
1106	56-57	0.07077795719487767
1106	58-59	0.21719350432221063
1106	60-61	0.20522979136411834
1106	62-63	-0.008075737061211896
1106	64-65	-0.7887929331287538
1106	66-67	-0.38208546008791444
1106	68-69	-0.05192450663240322
1106	70-71	-0.463842412333932
1106	72-73	-0.5008444096933289
1106	74-75	-0.2169232358907749
1106	76-77	-0.2455747112843305
1106	78-79	-0.07091614493087661
1106	80-81	-0.06745391102347753
1106	82-83	-0.1312750426367728
1106	84-85	-0.28265592923460403
1106	86-87	-0.07800830078591048
1106	88-89	-0.1573872248970254
1106	90-91	-0.7929853089828676
1106	92-93	-0.9792145776417733
1106	94-95	-1.4647914870276573
1106	96-97	-1.0585245008009032
1106	98-99	-0.9216338672197715
1106	100-101	-0.7788210910781537
1107	1	0.08605325994463442
1107	2	0.026826943907330758
1107	3	0.05652023570933551
1107	4	0.21497325159867842
1107	5	0.1259317991739195
1107	6	0.08989118007942665
1107	7	0.12334964432400852
1107	8	0.08747317425983425
1107	9	0.10894999813726258
1107	10-11	0.05990787505340478
1107	12-13	0.06294400659041344
1107	14-15	0.10192328457192446
1107	16-17	0.04253301862471659
1107	18-19	0.18319090023348394
1107	20-21	0.14692024551405325
1107	22-23	0.19596358290478122
1107	24-25	0.24533180260142728
1107	26-27	0.2418716905095053
1107	28-29	0.2642685640799769
1107	30-31	0.30251835892489964
1107	32-33	0.4291755329749023
1107	34-35	0.33106943946935985
1107	36-37	0.24973125275213448
1107	38-39	0.22631059861259217
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2216	62-63	-1.7244819059020173
2216	64-65	-1.63569711594403
2216	66-67	-1.6105723574562347
2216	68-69	-1.7266209356817228
2216	70-71	-1.65577336206389
2216	72-73	-1.7376361578582404
2216	74-75	-1.5015216235776165
2216	76-77	-1.5474401715889812
2216	78-79	-1.5531609367779957
2216	80-81	-1.5867103246009613
2216	82-83	-1.5805716876418998
2216	84-85	-1.5766286892087145
2216	86-87	-1.5596354969342698
2216	88-89	-1.6536738048085944
2216	90-91	-1.6943438305776901
2216	92-93	-1.6386838681500109
2216	94-95	-1.6953641661254046
2216	96-97	-1.808751130586483
2216	98-99	-1.9674214987044536
2216	100-101	-1.8083609915942453
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6307.0
3	5202.0
4	3573.0
5	6300.0
6	10169.0
7	18041.0
8	31714.0
9	48527.0
10	57025.0
11	57130.0
12	60899.0
13	66231.0
14	70041.0
15	74570.0
16	79605.0
17	86613.0
18	95348.0
19	106298.0
20	121290.0
21	141339.0
22	169643.0
23	205516.0
24	250045.0
25	295224.0
26	357650.0
27	445706.0
28	483718.0
29	479893.0
30	572154.0
31	690319.0
32	841340.0
33	1044277.0
34	1326140.0
35	1735261.0
36	2444526.0
37	4285744.0
38	3334150.0
39	5676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.657338389955918	12.805229431691952	18.632702648062498	39.90472953028963
2	22.10305608125685	23.66190293790785	31.055412628445467	23.179628352389837
3	25.36751133704068	23.133166307472067	28.803474042453924	22.69584831303333
4	25.533514564404307	28.6179794235432	18.91001726678639	26.938488745266103
5	30.46273377389011	30.40440884922005	20.04489592274918	19.087961454140654
6	24.84065784695366	32.430249845015126	19.629133267697753	23.099959040333463
7	19.305999183421996	18.602993337113272	38.731114147701184	23.35989333176355
8	21.07436985176504	24.178151825039908	24.560005457111657	30.187472866083393
9	24.14426574508917	20.127888118802872	28.02556688446391	27.702279251644047
10-11	26.964602553316404	29.719949792717586	19.214398375857904	24.101049278108107
12-13	22.7018236910923	23.952634570568545	26.581796997169892	26.76374474116926
14-15	22.99810280737127	25.795659598542535	25.546521466725768	25.659716127360426
16-17	25.520284796521604	24.06016665600201	24.4832203700193	25.936328177457085
18-19	23.601590278704478	24.513324679648253	26.218868460738527	25.666216580908742
20-21	25.41557403860539	24.54557337287353	26.352622702609825	23.68622988591126
22-23	23.771058432168086	28.421628241987527	24.404229619928245	23.403083705916146
24-25	23.578351813067673	24.712067256912423	26.28237897850586	25.42720195151404
26-27	23.563634714778342	24.60163761975159	24.372103068776987	27.46262459669308
28-29	25.46267611814027	26.36371185054785	24.38984867382944	23.78376335748244
30-31	23.43092413818066	24.75957171278194	26.473052710857896	25.3364514381795
32-33	23.423360292113014	26.683559415869635	24.57957536633701	25.31350492568034
34-35	23.670547713058422	24.523570257051627	28.176094490211558	23.629787539678393
36-37	25.391261271610972	26.391995833925126	24.520857012735668	23.69588588172823
38-39	25.52667620012547	24.612932357951976	24.431756202523918	25.428635239398634
40-41	23.625217890634943	26.495429116306756	26.32657824614008	23.55277474691822
42-43	23.357149290808398	24.64908689797641	26.65682905133063	25.336934759884567
44-45	25.382536744072297	24.555497933195465	24.688973417399467	25.37299190533277
46-47	23.699159369636984	26.30742862700824	26.3606652149347	23.63274678842008
48-49	25.35309606307181	24.577154429784116	26.511685691406385	23.558063815737686
50-51	23.59361419893798	24.61670081297575	24.539891194702175	27.249793793384093
52-53	25.4689343172878	24.650996413874125	26.298777662408163	23.581291606429915
54-55	23.35246776550749	24.54769463408161	26.679719058928104	25.420118541482793
56-57	23.509663918952086	24.513485326345993	26.641221277265043	25.33562947743688
58-59	25.514301450927462	24.454537427254255	26.386820319626846	23.644340802191437
60-61	23.41243344421903	24.698238033085133	26.506266729060172	25.383061793635665
62-63	25.21125177271607	24.80662205782828	26.625737997784938	23.35638817167071
64-65	23.700928007293122	28.30803088359269	24.580022158578014	23.411018950536175
66-67	23.448026977700817	28.409188312314637	24.71052100898494	23.432263700999602
68-69	23.74653486336637	28.248420788652073	24.5451694319811	23.45987491600045
70-71	23.689447986506774	28.337586095184044	24.473974907230094	23.49899101107909
72-73	23.598975081245136	28.388567530066318	24.63554538600613	23.376912002682417
74-75	24.287249311447347	27.291305751187128	24.825403252510142	23.596041684855383
76-77	24.205146529613085	26.541992016786587	24.899270151090796	24.353591302509535
78-79	24.055247488167474	26.25002709662767	25.10882154827247	24.58590386693239
80-81	24.64660170428728	25.88596289874474	24.944194773472933	24.523240623495045
82-83	24.340556681179297	25.703700191812516	25.44766343525073	24.508079691757462
84-85	24.744808832955105	25.780920336252066	25.636143469861988	23.838127360930837
86-87	24.342457617770513	26.8784415140535	25.015557348360073	23.763543519815915
88-89	24.42585733139733	25.60392184621105	25.3790048332146	24.591215989177012
90-91	24.242053188228404	25.722387200200654	24.885002789449832	25.15055682212111
92-93	24.854957134793647	26.419108941075383	24.88915302525471	23.836780898876263
94-95	24.658316453418532	25.815480206050285	25.226622319786994	24.29958102074419
96-97	24.441362875917225	26.277730045729868	24.960103430385256	24.32080364796765
98-99	24.64420671232943	25.737984933798685	25.7653646923929	23.85244366147898
100-101	25.473718236519648	26.273116711809447	24.242560603020365	24.01060444865054
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	716.0
1	446.5
2	164.5
3	157.5
4	160.5
5	159.5
6	149.0
7	157.5
8	171.5
9	170.5
10	203.0
11	235.0
12	271.5
13	348.0
14	464.5
15	619.0
16	959.0
17	1524.5
18	2460.0
19	4048.0
20	6599.5
21	10727.5
22	17030.5
23	26173.0
24	38347.0
25	53681.0
26	72954.0
27	94753.0
28	117975.5
29	142249.5
30	167951.5
31	194989.0
32	222917.0
33	250669.5
34	278651.5
35	311811.5
36	349528.5
37	388500.5
38	424251.5
39	459863.0
40	495119.0
41	529867.0
42	569874.0
43	614912.0
44	648894.5
45	676265.0
46	715415.5
47	733154.0
48	730287.0
49	721229.0
50	711071.5
51	702780.5
52	680666.5
53	659735.0
54	638126.5
55	620479.0
56	599348.5
57	567054.5
58	543831.0
59	523670.0
60	498845.0
61	469423.5
62	434667.5
63	402043.5
64	371064.5
65	331490.5
66	284143.5
67	243541.0
68	207557.0
69	166293.0
70	124602.0
71	88875.5
72	62031.5
73	41391.0
74	26422.5
75	15988.0
76	9066.5
77	4935.5
78	2762.0
79	1448.0
80	765.0
81	445.0
82	271.5
83	159.5
84	109.0
85	70.5
86	43.0
87	35.5
88	25.5
89	13.5
90	11.0
91	8.5
92	5.0
93	4.0
94	3.0
95	3.0
96	3.0
97	2.5
98	1.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031019423857084134
2	0.001929081015635301
3	7.507506014456971E-4
4	1.1435274061755649E-4
5	9.446530746667712E-5
6	0.015795593780085958
7	0.0
8	0.0
9	7.706380345965764E-4
10-11	9.222797123720318E-4
12-13	5.7176370308778246E-5
14-15	3.206848595579302E-4
16-17	1.8395875664563437E-4
18-19	0.0
20-21	4.971858287719848E-6
22-23	9.943716575439696E-6
24-25	0.0
26-27	5.469044116491833E-4
28-29	0.006707036830134076
30-31	0.0017202629675510677
32-33	0.20915613444779857
34-35	0.008255770686758809
36-37	0.006102956048176114
38-39	9.048782083650124E-4
40-41	9.421671455229112E-4
42-43	0.061976699485571767
44-45	0.40488576558960976
46-47	0.11751981434683405
48-49	0.17186471136075585
50-51	0.002428752773551146
52-53	0.002746951703965216
54-55	7.979832551790356E-4
56-57	3.206848595579302E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.007519935660176271
82-83	0.00814638980442897
84-85	0.007783444149425423
86-87	0.006590198160372658
88-89	0.013302206848794454
90-91	0.012964120485229503
92-93	0.020198174293861882
94-95	0.016200800230535124
96-97	0.007519935660176271
98-99	0.008111586796414933
100-101	0.00817622095415529
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.0113204E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.78981324943107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.58351317487734	38.388725188879334
2	13.194029243807059	13.402445626009857
3	4.454136956454333	6.786743526171146
4	2.050265895739132	4.165304878250723
5	1.154994368304261	2.9330974135159007
6	0.7092654917100933	2.1614077120932924
7	0.48390209150671376	1.7204107801044568
8	0.35282439860340853	1.433590825192796
9	0.2625344164198716	1.2000666583362285
>10	1.5795181368970783	14.995747611328941
>50	0.11965385011265736	4.159538257169535
>100	0.05414814543968277	4.595956796390115
>500	0.001007736285281394	0.32903764348634423
>1k	1.7668867690519372E-4	0.14066578816372458
>5k	9.80172206701123E-6	0.03328475556286108
>10k+	1.9603444134022452E-5	3.5539765393448746
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	693328	3.447128562908227	TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG	20569	0.10226615312010955	TruSeq Adapter, Index 3 (100% over 49bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.11538191528311452	4.971858287719848E-6	0.0	0.0	0.0
2	0.11584429810387246	2.485929143859924E-5	0.0	7.457787431579773E-5	0.0
3	0.11591887597818826	2.9831149726319087E-5	0.0	7.457787431579773E-5	0.0
4	0.11619730004230057	3.4803008014038935E-5	0.0	8.452159089123741E-5	0.0
5	0.11626193420004093	3.4803008014038935E-5	0.0	8.452159089123741E-5	0.0
6	0.11658510498874272	3.4803008014038935E-5	0.0	8.452159089123741E-5	0.0
7	0.11674420445394976	5.469044116491833E-5	0.0	8.949344917895727E-5	0.0
8	0.11711709382552875	6.463415774035803E-5	0.0	9.446530746667712E-5	0.0
9	0.11767891381204108	6.960601602807787E-5	0.0	9.446530746667712E-5	0.0
10-11	0.11841723476776747	7.706380345965766E-5	0.0	9.695123661053703E-5	0.0
12-13	0.11926990846411142	1.0192309489825689E-4	0.0	1.0192309489825689E-4	0.0
14-15	0.12074157851727652	1.242964571929962E-4	0.0	1.044090240421168E-4	0.0
16-17	0.1223052279487644	1.242964571929962E-4	0.0	1.0689495318597673E-4	0.0
18-19	0.12441329586275762	1.242964571929962E-4	0.0	1.143527406175565E-4	0.0
20-21	0.1528871282765292	1.2926831548071605E-4	0.0	1.2181052804913626E-4	2.485929143859924E-6
22-23	0.15447066514116797	1.367261029122958E-4	0.0	1.2926831548071605E-4	4.971858287719848E-6
24-25	0.15577329201255058	1.566135360631752E-4	0.0	1.441838903438756E-4	4.971858287719848E-6
26-27	0.15723253241999635	1.64071323494755E-4	4.971858287719848E-6	1.4915574863159545E-4	7.457787431579773E-6
28-29	0.15865945574857193	1.6904318178247483E-4	4.971858287719848E-6	1.4915574863159545E-4	9.943716575439696E-6
30-31	0.160238020754923	1.7898689835791454E-4	4.971858287719848E-6	1.5164167777545538E-4	9.943716575439696E-6
32-33	0.16178675461154773	1.8893061493335423E-4	4.971858287719848E-6	1.5412760691931528E-4	1.4915574863159544E-5
34-35	0.16322859351498648	1.8893061493335423E-4	4.971858287719848E-6	1.5909946520703514E-4	1.4915574863159544E-5
36-37	0.1649264831202428	2.0881804808423364E-4	4.971858287719848E-6	1.5909946520703514E-4	1.4915574863159544E-5
38-39	0.1678101609271203	2.28705481235113E-4	4.971858287719848E-6	1.64071323494755E-4	1.4915574863159544E-5
40-41	0.17079327589975224	2.28705481235113E-4	4.971858287719848E-6	1.64071323494755E-4	1.4915574863159544E-5
42-43	0.17284913930172438	2.3119141037897294E-4	4.971858287719848E-6	1.740150400701947E-4	1.7401504007019467E-5
44-45	0.17532512472900885	2.6599441839301183E-4	4.971858287719848E-6	1.8147282750177447E-4	1.9887433150879393E-5
46-47	0.17783839909345123	2.9333963897547105E-4	4.971858287719848E-6	1.8395875664563437E-4	1.9887433150879393E-5
48-49	0.18039393425333924	3.0825521383863057E-4	4.971858287719848E-6	1.864446857894943E-4	1.9887433150879393E-5
50-51	0.1831085688784343	3.206848595579302E-4	4.971858287719848E-6	1.8893061493335423E-4	1.9887433150879393E-5
52-53	0.18607925420534688	3.2565671784565007E-4	4.971858287719848E-6	1.939024732210741E-4	1.9887433150879393E-5
54-55	0.18937559625010514	3.405722927088096E-4	4.971858287719848E-6	1.939024732210741E-4	1.9887433150879393E-5
56-57	0.19311940554075818	3.579737967158291E-4	4.971858287719848E-6	1.939024732210741E-4	1.9887433150879393E-5
58-59	0.19853127328694126	3.7786122986670846E-4	4.971858287719848E-6	1.9887433150879392E-4	1.9887433150879393E-5
60-61	0.20472869464258403	3.828330881544283E-4	4.971858287719848E-6	1.9887433150879392E-4	1.9887433150879393E-5
62-63	0.21362086319017098	4.0023459216144777E-4	4.971858287719848E-6	2.1130397722809354E-4	1.9887433150879393E-5
64-65	0.2679533305583735	4.25093883600047E-4	4.971858287719848E-6	2.2373362294739315E-4	1.9887433150879393E-5
66-67	0.27740234723418505	4.4249538760706645E-4	4.971858287719848E-6	2.3367733952283287E-4	2.485929143859924E-5
68-69	0.2852156225333368	4.474672458947863E-4	4.971858287719848E-6	2.510788435298523E-4	2.485929143859924E-5
70-71	0.29559189077980813	4.5243910418250616E-4	4.971858287719848E-6	2.709662766807317E-4	2.7345220582459162E-5
72-73	0.31258321647809073	4.6735467904566574E-4	4.971858287719848E-6	2.9333963897547105E-4	2.9831149726319087E-5
74-75	0.3373206973886408	4.748124664772455E-4	4.971858287719848E-6	3.0328335555091077E-4	2.9831149726319087E-5
76-77	0.3644247828441456	4.872421121965451E-4	4.971858287719848E-6	3.132270721263504E-4	2.9831149726319087E-5
78-79	0.3939899381520716	4.872421121965451E-4	4.971858287719848E-6	3.132270721263504E-4	2.9831149726319087E-5
80-81	0.436417290850329	4.872421121965451E-4	4.971858287719848E-6	3.2814264698951E-4	2.9831149726319087E-5
82-83	0.5113755123251373	5.071295453474245E-4	4.971858287719848E-6	3.2814264698951E-4	2.9831149726319087E-5
84-85	0.6277145103286379	5.24531049354444E-4	4.971858287719848E-6	3.3062857613336987E-4	2.9831149726319087E-5
86-87	0.7628197874391369	5.319888367860238E-4	4.971858287719848E-6	3.579737967158291E-4	2.9831149726319087E-5
88-89	0.8966075221033905	5.319888367860238E-4	4.971858287719848E-6	3.704034424351287E-4	3.4803008014038935E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGAAG	106690	0.0	66.19862	3
CGGAAGA	108410	0.0	65.170555	4
ATCGGAA	109625	0.0	64.756195	2
GATCGGA	111700	0.0	63.672974	1
AGAGCAC	112515	0.0	63.084587	8
GAGCACA	112795	0.0	63.05423	9
AAGAGCA	120360	0.0	58.944576	7
GAAGAGC	122915	0.0	57.75839	6
GGAAGAG	133430	0.0	53.22767	5
CGTATGC	81385	0.0	44.45848	44-45
CTCGTAT	80140	0.0	44.455338	42-43
TATGCCG	81830	0.0	44.226776	46-47
ATCTCGT	81700	0.0	43.409298	40-41
TCGTATG	80890	0.0	43.123375	42-43
ACTTAGG	85790	0.0	42.58078	32-33
TTAGGCA	86260	0.0	42.410053	34-35
TCTCGTA	81250	0.0	42.103794	40-41
CCGTCTT	85745	0.0	41.95427	50-51
TGCCGTC	85550	0.0	41.9516	48-49
ATGCCGT	84245	0.0	41.190304	46-47
>>END_MODULE