FastQCFastQC Report
Wed 25 May 2016
SRR1033292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23850053
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC345680.14493888126789486Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA451500.047.2136731
ATCGGAA450050.046.667222
TCGGAAG439500.045.022663
CGGAAGA460200.037.1812444
AAGAGCG354700.036.575047
CGCCGTA134400.034.47886746-47
GATCTCG327100.033.9549134-35
CCGTATC162300.032.64042748-49
AGAGCGT265600.032.302818
AGACGGC119800.032.1250539
GAGCGTC269450.031.2859559
ATCGGGA73100.030.8597872
GATCGGG77400.030.3571321
AGAGCGG140150.030.2712578
TAGATCT277900.030.0803332-33
GGCGCCG210800.029.63580744-45
TCGGGGG312350.029.45557438-39
GAGCGGC145250.028.8399059
AAGGGCG94650.028.5322287
GCCGGAT146600.028.39666746-47
Produced by FastQC (version 0.11.5)