##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033292_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23850053
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.868147546674216	33.0	33.0	33.0	27.0	33.0
2	30.924484570327788	33.0	33.0	33.0	27.0	33.0
3	30.945991314987854	33.0	33.0	33.0	27.0	33.0
4	34.63284270269756	37.0	37.0	37.0	33.0	37.0
5	34.56157980026292	37.0	37.0	37.0	27.0	37.0
6	34.62891529004149	37.0	37.0	37.0	33.0	37.0
7	34.48624218990205	37.0	37.0	37.0	27.0	37.0
8	34.55473067502198	37.0	37.0	37.0	33.0	37.0
9	34.41275958590113	37.0	37.0	37.0	27.0	37.0
10-11	34.48478756001087	37.0	37.0	37.0	30.0	37.0
12-13	34.404879729198086	37.0	37.0	37.0	30.0	37.0
14-15	35.814772382266824	40.0	37.0	40.0	27.0	40.0
16-17	35.709611232310465	40.0	37.0	40.0	27.0	40.0
18-19	35.69637434348678	40.0	37.0	40.0	27.0	40.0
20-21	35.58868265408047	40.0	37.0	40.0	27.0	40.0
22-23	35.52608101122459	37.0	37.0	40.0	27.0	40.0
24-25	34.67430063991891	37.0	35.0	40.0	24.5	40.0
26-27	33.69300368011761	37.0	33.0	40.0	15.0	40.0
28-29	33.266919239131255	37.0	33.0	40.0	15.0	40.0
30-31	33.18566721843344	37.0	33.0	40.0	15.0	40.0
32-33	33.007492163644244	37.0	33.0	40.0	15.0	40.0
34-35	32.86091961724362	37.0	33.0	40.0	15.0	40.0
36-37	32.7465994310369	37.0	33.0	40.0	15.0	40.0
38-39	32.49709422029376	37.0	33.0	40.0	6.0	40.0
40-41	32.16335133511024	37.0	33.0	40.0	6.0	40.0
42-43	31.832170561633554	37.0	33.0	40.0	6.0	40.0
44-45	31.69673522318797	37.0	33.0	40.0	6.0	40.0
46-47	31.50768337915224	37.0	33.0	40.0	6.0	40.0
48-49	31.35616843283325	37.0	33.0	40.0	6.0	40.0
50-51	31.100475143598214	37.0	27.0	40.0	2.0	40.0
52-53	30.875241912460318	37.0	27.0	40.0	2.0	40.0
54-55	30.721448627388796	37.0	27.0	38.5	2.0	40.0
56-57	30.5213314410664	37.0	27.0	37.0	2.0	40.0
58-59	30.289360551945105	37.0	27.0	37.0	2.0	40.0
60-61	30.05421973276118	37.0	27.0	37.0	2.0	40.0
62-63	29.77809816607116	37.0	27.0	37.0	2.0	40.0
64-65	29.50131624445447	33.0	27.0	37.0	2.0	40.0
66-67	29.224865873463678	33.0	27.0	37.0	2.0	40.0
68-69	29.207773877902916	33.0	27.0	37.0	2.0	40.0
70-71	29.14707772347508	33.0	27.0	37.0	2.0	38.5
72-73	28.969159355746505	33.0	27.0	37.0	2.0	37.0
74-75	28.72271260780846	33.0	27.0	37.0	2.0	37.0
76-77	28.4916531422383	33.0	27.0	37.0	2.0	37.0
78-79	28.249897012807477	33.0	27.0	37.0	2.0	37.0
80-81	28.032685084599184	33.0	27.0	37.0	2.0	37.0
82-83	27.8008025600614	33.0	27.0	37.0	2.0	37.0
84-85	27.61677818913023	33.0	27.0	37.0	2.0	37.0
86-87	27.404141198344508	33.0	27.0	37.0	2.0	37.0
88-89	27.155865125331168	33.0	24.5	37.0	2.0	37.0
90-91	26.931475120830967	33.0	22.0	37.0	2.0	37.0
92-93	26.689242179042537	33.0	22.0	37.0	2.0	37.0
94-95	26.43112619078876	33.0	22.0	37.0	2.0	37.0
96-97	26.080343175757303	33.0	22.0	37.0	2.0	37.0
98-99	25.62789164451752	33.0	15.0	37.0	2.0	37.0
100-101	22.72736423269164	30.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6082820913029003
1101	2	0.5941896688651127
1101	3	0.5774676651168953
1101	4	0.6561769422278303
1101	5	0.6780592284180713
1101	6	0.7240264763182651
1101	7	0.7791806515385602
1101	8	0.7725048824590601
1101	9	0.7946456445267032
1101	10-11	0.7718074454289621
1101	12-13	0.7363010780183359
1101	14-15	1.1629466982973042
1101	16-17	1.207242270863361
1101	18-19	1.133504212970827
1101	20-21	1.185444201151192
1101	22-23	1.238833652032163
1101	24-25	1.3326680565127873
1101	26-27	1.3659130098163104
1101	28-29	1.4799941814855302
1101	30-31	1.3511756985523462
1101	32-33	1.5154842583577164
1101	34-35	1.5218580248412081
1101	36-37	1.4730203163887126
1101	38-39	1.5451013537178255
1101	40-41	1.5644105149798264
1101	42-43	1.6115958597974185
1101	44-45	1.5362986452347513
1101	46-47	1.5639904378477993
1101	48-49	1.5280420503785344
1101	50-51	1.6127031262562532
1101	52-53	1.6009142433362484
1101	54-55	1.6081053808178183
1101	56-57	1.5466191942788505
1101	58-59	1.5632445093464682
1101	60-61	1.5101469762085031
1101	62-63	1.545480380361262
1101	64-65	1.5918646845040634
1101	66-67	1.5708365948210208
1101	68-69	1.570391845700982
1101	70-71	1.6109312172172388
1101	72-73	1.576335034371386
1101	74-75	1.6075935302259516
1101	76-77	1.6310399130862585
1101	78-79	1.6587418492932677
1101	80-81	1.7099587085536605
1101	82-83	1.7409632453476576
1101	84-85	1.7779073787166446
1101	86-87	1.7626407602448815
1101	88-89	1.8016103511608037
1101	90-91	1.825088203032891
1101	92-93	1.8725794229715689
1101	94-95	1.8491463344071128
1101	96-97	1.9347282288313146
1101	98-99	2.075348311215727
1101	100-101	2.0549989345944013
1102	1	0.5517529831758452
1102	2	0.5764495739743936
1102	3	0.5542437551495354
1102	4	0.6256386961088438
1102	5	0.6242453258312537
1102	6	0.6725925134082971
1102	7	0.6602125321681171
1102	8	0.7069892139563976
1102	9	0.7344099105415225
1102	10-11	0.6626685147371134
1102	12-13	0.6573994489075545
1102	14-15	1.0655250534350529
1102	16-17	1.0350252161917268
1102	18-19	1.0365803501371502
1102	20-21	1.0808275443692068
1102	22-23	1.1141840174237387
1102	24-25	1.226663156477997
1102	26-27	1.269666347338486
1102	28-29	1.3716103933819284
1102	30-31	1.339662525588949
1102	32-33	1.4579746951485717
1102	34-35	1.3569208956798917
1102	36-37	1.3096291308903858
1102	38-39	1.3174276138248118
1102	40-41	1.3734190288430383
1102	42-43	1.3507083291973458
1102	44-45	1.2922697189598793
1102	46-47	1.2821002828428902
1102	48-49	1.2985181188870918
1102	50-51	1.3838883929463215
1102	52-53	1.332572302309984
1102	54-55	1.3470812081616046
1102	56-57	1.2656232676509624
1102	58-59	1.301143906166093
1102	60-61	1.32329268822539
1102	62-63	1.399995558901324
1102	64-65	1.4220059263082945
1102	66-67	1.4200994112416296
1102	68-69	1.438585422054242
1102	70-71	1.4492231108221176
1102	72-73	1.413536307828938
1102	74-75	1.389906606423196
1102	76-77	1.4552808390556287
1102	78-79	1.531383849534894
1102	80-81	1.5496545628504919
1102	82-83	1.5512280426181917
1102	84-85	1.549486387804297
1102	86-87	1.5540970477669127
1102	88-89	1.6586726819238358
1102	90-91	1.6649368123051964
1102	92-93	1.647498456797166
1102	94-95	1.6847430919106259
1102	96-97	1.6899230243216117
1102	98-99	1.795365059496966
1102	100-101	1.803475197938571
1103	1	0.3496145078862085
1103	2	0.35514240921195395
1103	3	0.32253418238690656
1103	4	0.39278251526111063
1103	5	0.3406615378413278
1103	6	0.39840636635339877
1103	7	0.4435747526175646
1103	8	0.4817235471370225
1103	9	0.5240005706110722
1103	10-11	0.5126063532471221
1103	12-13	0.4199117474335594
1103	14-15	0.6484175779103936
1103	16-17	0.7423865212292782
1103	18-19	0.6198453197270979
1103	20-21	0.5894267938365871
1103	22-23	0.59540496179347
1103	24-25	0.6610679169906817
1103	26-27	0.6561600408277286
1103	28-29	0.658012005228052
1103	30-31	0.6766808644322353
1103	32-33	0.8178931463507624
1103	34-35	0.8350265481487185
1103	36-37	0.7784908961245236
1103	38-39	0.8439249840187273
1103	40-41	0.8677989963668225
1103	42-43	0.7858754256666174
1103	44-45	0.696059135283619
1103	46-47	0.6368350828554625
1103	48-49	0.6157790026745893
1103	50-51	0.42914825364421105
1103	52-53	0.49296529997296545
1103	54-55	0.4629463942020351
1103	56-57	0.35041530277641897
1103	58-59	0.359730303267348
1103	60-61	0.3567782325064677
1103	62-63	0.4167085833555326
1103	64-65	0.3993096737238524
1103	66-67	0.4277196339024769
1103	68-69	0.4366286345414174
1103	70-71	0.4666033373434004
1103	72-73	0.2621563035517127
1103	74-75	0.3160166013462238
1103	76-77	0.4032697942976604
1103	78-79	0.38558558607563853
1103	80-81	0.4228714291731066
1103	82-83	0.4968133059411741
1103	84-85	0.39215994663181775
1103	86-87	0.3767528287330748
1103	88-89	0.2748865091347241
1103	90-91	0.4088511176124179
1103	92-93	0.3973552003905425
1103	94-95	0.4703527511331558
1103	96-97	0.5065121657184228
1103	98-99	0.4824400600420198
1103	100-101	0.5412274173562146
1104	1	0.21769774193163371
1104	2	0.2849171199837386
1104	3	0.2674262766104327
1104	4	0.318917783678458
1104	5	0.3149510860387892
1104	6	0.331722162907532
1104	7	-1.1632747441423348
1104	8	0.0529821235997332
1104	9	0.33334308489784803
1104	10-11	0.37291335164129435
1104	12-13	0.401096591951422
1104	14-15	0.6040477161931221
1104	16-17	0.6690923940700273
1104	18-19	0.6684493407952417
1104	20-21	0.6789900688526203
1104	22-23	0.7002627410832716
1104	24-25	0.7614734971222532
1104	26-27	0.8321020853408285
1104	28-29	0.9322268489289982
1104	30-31	0.8276986057664928
1104	32-33	1.0445901473893358
1104	34-35	0.9247160488028072
1104	36-37	0.9081140110388048
1104	38-39	0.9631109879585082
1104	40-41	0.8536905097331342
1104	42-43	0.8823707034833888
1104	44-45	0.7602839920877855
1104	46-47	0.6404648791839449
1104	48-49	0.7240139675371502
1104	50-51	0.5209979781983414
1104	52-53	0.7442908566856516
1104	54-55	0.701498874467795
1104	56-57	0.6973214294085643
1104	58-59	0.7357043603266327
1104	60-61	0.6783495703343441
1104	62-63	0.6993473144831412
1104	64-65	0.8132577178657527
1104	66-67	0.7303393540111855
1104	68-69	0.5654746949089322
1104	70-71	0.6231002332305664
1104	72-73	0.5846388868245462
1104	74-75	0.5121959447760993
1104	76-77	0.5824243255298143
1104	78-79	0.5018013254491009
1104	80-81	0.5475280903915731
1104	82-83	0.5245395491628528
1104	84-85	0.5579164007333368
1104	86-87	0.6131538340518183
1104	88-89	0.5799777424368564
1104	90-91	0.6199137595404132
1104	92-93	0.6786592713840385
1104	94-95	0.6792005897484223
1104	96-97	0.6479784475338235
1104	98-99	0.6150215620277386
1104	100-101	0.7200442853505926
1105	1	0.10522852328490018
1105	2	0.11978682133940666
1105	3	0.06997807470379769
1105	4	0.13817476788508998
1105	5	-0.025696794254258748
1105	6	0.1232780765666206
1105	7	-1.7829625271410237
1105	8	-0.47452246009387977
1105	9	0.02947665614264139
1105	10-11	0.1732780986889324
1105	12-13	0.26821240240133903
1105	14-15	0.3935833445175163
1105	16-17	0.49712539204533357
1105	18-19	0.49222332384150747
1105	20-21	0.4717591639612735
1105	22-23	0.47213384398037306
1105	24-25	0.5850820274544617
1105	26-27	0.486845318045539
1105	28-29	0.5618749554932876
1105	30-31	0.4507712378159212
1105	32-33	0.5882855459434069
1105	34-35	0.5698642032592574
1105	36-37	0.2787391869622411
1105	38-39	0.21258266050273278
1105	40-41	0.2995744903738924
1105	42-43	0.3569452566024225
1105	44-45	0.33692024853704083
1105	46-47	0.2627505233756935
1105	48-49	0.3293556156224575
1105	50-51	0.28351444368212597
1105	52-53	0.34253509723426134
1105	54-55	0.088407824049483
1105	56-57	0.2070058897823408
1105	58-59	0.24649644666005344
1105	60-61	0.1671799296430514
1105	62-63	0.15707531480242665
1105	64-65	0.22642590575405563
1105	66-67	0.18560541035606093
1105	68-69	0.05047934623943462
1105	70-71	0.32497445201879316
1105	72-73	0.271882406556589
1105	74-75	0.2841771117449845
1105	76-77	0.29632917726021546
1105	78-79	0.28689749315761404
1105	80-81	0.316566742216672
1105	82-83	0.17186163693587275
1105	84-85	0.3045159352606568
1105	86-87	0.37081239233633667
1105	88-89	0.43417613656534826
1105	90-91	0.30847601702797434
1105	92-93	0.3866440336046395
1105	94-95	0.3045670772718694
1105	96-97	0.3367507584225642
1105	98-99	0.3572041233582688
1105	100-101	0.3746210004182764
1106	1	-0.4603899402305487
1106	2	-0.42758275719445393
1106	3	-0.45186669996024165
1106	4	-0.46853331558313016
1106	5	-0.7418153380168135
1106	6	-0.612831547229689
1106	7	-0.3717017984465869
1106	8	-0.4088705715983494
1106	9	-0.008866141038922137
1106	10-11	-0.21049065177442117
1106	12-13	-0.4853625316063912
1106	14-15	-0.6909256527086427
1106	16-17	-0.6177745045522158
1106	18-19	-0.7625083023088877
1106	20-21	-0.5866235494808691
1106	22-23	-0.5436502372563154
1106	24-25	-0.37456139386222276
1106	26-27	-0.5649078097133824
1106	28-29	-0.7431124860378802
1106	30-31	-0.9147717105904789
1106	32-33	-1.1626475247092571
1106	34-35	-0.7956620100267884
1106	36-37	-0.9475298126609175
1106	38-39	-0.9400499538412568
1106	40-41	-1.0033040317975335
1106	42-43	-0.686514934449967
1106	44-45	-0.8655754433292664
1106	46-47	-1.010476296140368
1106	48-49	-1.2620390859139654
1106	50-51	-1.0142211236739804
1106	52-53	-1.536735979308986
1106	54-55	-1.5279346421863096
1106	56-57	-1.1664604761786883
1106	58-59	-1.0224470080499302
1106	60-61	-1.0615316281221432
1106	62-63	-0.8014985750424195
1106	64-65	-1.2069006618684597
1106	66-67	-1.157885809125709
1106	68-69	-1.0847061752993312
1106	70-71	-1.0412647181292485
1106	72-73	-1.3660677704441788
1106	74-75	-1.4829125691286045
1106	76-77	-1.1568211305075842
1106	78-79	-1.3429545828617897
1106	80-81	-1.5010303239707632
1106	82-83	-1.3327902626032575
1106	84-85	-1.2232613886535155
1106	86-87	-1.2152554038427077
1106	88-89	-1.7251667405464985
1106	90-91	-1.3070199847831745
1106	92-93	-1.5069052801671674
1106	94-95	-1.284083064525177
1106	96-97	-1.2098851338777585
1106	98-99	-1.4680760603492224
1106	100-101	-1.2004303455108492
1107	1	0.1458899707139274
1107	2	0.1631524481977067
1107	3	0.18454438681509444
1107	4	0.22879024897975597
1107	5	0.23098363127557775
1107	6	0.21747999565117482
1107	7	0.3666117549425394
1107	8	0.30982950618621174
1107	9	0.24960799079065055
1107	10-11	0.29314641508978667
1107	12-13	0.2678876469183322
1107	14-15	0.38791106910949225
1107	16-17	0.4253425507019415
1107	18-19	0.3507908685090442
1107	20-21	0.42066742513640776
1107	22-23	0.34578085039158424
1107	24-25	0.4502927685612548
1107	26-27	0.42334795908223555
1107	28-29	0.5080471617456581
1107	30-31	0.37931530639530564
1107	32-33	0.5442053833505653
1107	34-35	0.40749259927927994
1107	36-37	0.2531512487084129
1107	38-39	0.2702897528831443
1107	40-41	0.34681485470549944
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2216	86-87	-0.8975378587910008
2216	88-89	-0.867429568789774
2216	90-91	-0.8671370938011869
2216	92-93	-0.9249043505424908
2216	94-95	-0.8991801772387547
2216	96-97	-0.9417847656972675
2216	98-99	-0.968261709026244
2216	100-101	-0.8706287175124565
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	512276.0
3	134241.0
4	112713.0
5	122981.0
6	173899.0
7	174678.0
8	157568.0
9	169880.0
10	170178.0
11	173034.0
12	168382.0
13	174244.0
14	188546.0
15	200915.0
16	156153.0
17	147739.0
18	149536.0
19	155401.0
20	167818.0
21	188940.0
22	217141.0
23	252380.0
24	298146.0
25	349627.0
26	403073.0
27	465726.0
28	539859.0
29	626471.0
30	734642.0
31	866814.0
32	1043779.0
33	1277490.0
34	1592248.0
35	2038701.0
36	2799751.0
37	4145827.0
38	2596277.0
39	2979.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.464416876629883	12.841959567938572	21.068141333528853	39.62548222190269
2	22.232813785621378	22.409742340635223	31.978270792754266	23.379173080989137
3	25.908025869585387	24.10759778562684	27.141015379045395	22.84336096574238
4	26.329837063651883	28.855924868698523	19.680560519391772	25.133677548257822
5	28.40314486380156	30.757590056224192	20.634500808445583	20.204764271528663
6	23.667267563654416	32.817397400100305	20.096860719023766	23.41847431722152
7	20.352175377763075	16.72532103947298	39.26171994144097	23.660783641322965
8	21.337534599416703	23.132749689266618	25.188266267804572	30.341449443512104
9	22.09402202699862	21.12192044234527	28.555311027374607	28.2287465032815
10-11	26.5939411220645	29.33506842127571	19.674591105990213	24.396399350669572
12-13	24.3381976412128	22.548973055758946	26.984127716521378	26.128701586506875
14-15	23.8035190152083	24.369269035101603	26.603487269817077	25.223724679873023
16-17	25.032355627249082	24.705561065494482	25.649251829339807	24.61283147791663
18-19	25.11761466934778	25.274204226271667	25.39478340734161	24.213397697038943
20-21	24.988565885931813	26.133424715344944	25.035296658595573	23.84271274012767
22-23	26.361890340296277	25.213990628101747	24.836822321414058	23.587296710187918
24-25	24.24809234082202	27.055749522088945	25.016932221280914	23.67922591580812
26-27	24.42875360823843	26.895849930167458	24.754996751662592	23.920399709931523
28-29	24.877892880466685	26.078756087300945	24.940550136375318	24.102800895857047
30-31	25.395168757545534	25.446855283187237	25.422281245079	23.73569471418823
32-33	24.311552542440637	26.228696435791566	25.81798426385416	23.641766757913643
34-35	24.98613514036292	26.331213320876927	24.787976366857723	23.89467517190243
36-37	23.867848716052304	25.465973275011365	26.01237420981708	24.653803799119252
38-39	23.878132017730948	25.35355804709419	25.97634176034647	24.791968174828394
40-41	25.98008417966272	25.24197197603707	24.877223667702822	23.900720176597385
42-43	25.38866995892985	25.290111385452306	25.53198798390618	23.789230671711664
44-45	25.831136265946725	25.313767301510058	25.15599115147093	23.69910528107229
46-47	25.03881832626685	25.181809006577428	25.03978878442352	24.739583882732198
48-49	24.18201017689751	25.140256359801526	25.039742958408446	25.637990504892517
50-51	25.33295847150879	25.28300689584877	25.368996170772963	24.01503846186948
52-53	24.123625628300783	26.135198640722706	25.791341484665303	23.949834246311205
54-55	23.687065609132414	25.988609042406512	25.490646553919483	24.833678794541594
56-57	23.740765333272858	25.320408482935136	27.1656341026186	23.773192081173406
58-59	23.890770307906063	26.81000473967796	25.260164858088686	24.03906009432729
60-61	23.67127554104948	27.694388522017515	24.948534770528454	23.685801166404552
62-63	23.620816735433987	27.742408450930668	24.978708144884116	23.65806666875123
64-65	23.75289731204552	27.612541030748954	24.95364722691423	23.680914430291296
66-67	23.726602468227487	27.506648358494836	25.042832505587754	23.723916667689927
68-69	24.28787968179861	26.81672553454813	25.098263657040427	23.79713112661283
70-71	24.593543241124568	26.2685740752659	25.169173666113466	23.968709017496067
72-73	24.33729291399338	26.362112530603422	25.353154041436675	23.94744051396652
74-75	24.60688825853224	26.269838137721695	25.31193051847972	23.811343085266348
76-77	24.792096670114923	25.826314718856313	25.31960133585944	24.061987275169326
78-79	24.51310290635358	25.765039885164292	25.59099407371332	24.130863134768813
80-81	24.77182617152462	25.935332879428262	25.338759370234154	23.95408157881296
82-83	24.700645164939527	26.021136883761702	25.278909957737966	23.999307993560805
84-85	24.69707112656371	26.01218801944178	25.40123174241888	23.889509111575634
86-87	25.024188968901623	25.80206206000536	25.35769158140312	23.816057389689902
88-89	24.87090896181882	25.812148183443167	25.212215924368575	24.104726930369438
90-91	24.719397941373824	25.933290775189977	25.232081933523553	24.115229349912646
92-93	24.73735581147796	26.077707904988863	25.31274009786337	23.872196185669814
94-95	24.94048645334298	25.9268951673643	25.22137979827541	23.911238581017308
96-97	24.755682416816487	25.888843741431085	25.453373857421308	23.90209998433112
98-99	24.963366015819837	25.95308089337533	25.202539055706453	23.88101403509838
100-101	25.539205896741475	25.751925052859537	24.546044476993266	24.162824573405718
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10458.0
1	6330.0
2	1769.0
3	1319.0
4	1283.5
5	1277.5
6	1401.5
7	1721.5
8	2191.5
9	2809.0
10	3704.0
11	4849.0
12	6153.0
13	7518.5
14	8895.5
15	10484.0
16	12078.0
17	13782.0
18	15901.5
19	18568.5
20	22233.0
21	27877.5
22	36257.5
23	47685.0
24	62478.0
25	80932.5
26	103937.0
27	130667.5
28	158599.5
29	187812.0
30	218547.5
31	250385.5
32	283343.5
33	315741.0
34	348222.5
35	386849.5
36	430283.0
37	472964.0
38	511099.0
39	545668.5
40	578366.0
41	608916.5
42	641770.5
43	679036.5
44	704099.0
45	724636.0
46	749906.5
47	763862.5
48	779081.5
49	789943.0
50	799588.5
51	809849.0
52	807656.0
53	803561.5
54	786958.5
55	765866.5
56	739667.0
57	700805.5
58	668118.5
59	640143.5
60	608175.5
61	569290.5
62	526114.0
63	486322.0
64	447147.5
65	399002.5
66	342719.0
67	293337.0
68	247273.5
69	195405.5
70	145611.5
71	102975.0
72	70371.5
73	45731.0
74	28514.5
75	17595.5
76	10987.0
77	6963.0
78	4621.0
79	3144.5
80	2180.0
81	1543.0
82	1141.0
83	870.5
84	643.0
85	461.5
86	328.0
87	240.5
88	166.5
89	119.5
90	80.0
91	48.5
92	43.0
93	41.5
94	34.0
95	24.0
96	15.0
97	15.0
98	15.5
99	14.5
100	26.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03928293157252103
2	0.034490489392203866
3	0.03354709526222017
4	0.034238917624208214
5	0.033555480987820026
6	0.030876241658666335
7	0.03406701024941118
8	0.07094323857477382
9	0.03827664450053843
10-11	0.03851773411153426
12-13	0.04194959231327494
14-15	0.04348837296084835
16-17	0.0432074511532532
18-19	0.0406791548848969
20-21	0.06113822891714328
22-23	0.042622546792663314
24-25	0.04565608302841088
26-27	0.04262045036126335
28-29	0.14245880292173774
30-31	0.15221349822576916
32-33	0.14758877055744907
34-35	0.11420100408162616
36-37	0.17028683332485675
38-39	0.3135611480611804
40-41	0.5996653340770354
42-43	0.6477931097260036
44-45	0.6396421844429444
46-47	0.6285394837487362
48-49	0.6194577429240933
50-51	0.6167575392809399
52-53	0.6256715655935859
54-55	0.6364011014986005
56-57	0.6244870818526064
58-59	0.6339000588384437
60-61	0.6312648445686893
62-63	0.6374157742961829
64-65	0.6346442919854308
66-67	0.6345730133178321
68-69	0.6482962532619948
70-71	0.648753275307187
72-73	0.6549838694278793
74-75	0.66246393666295
76-77	0.6701704184892168
78-79	0.6673528146876655
80-81	0.6681033371288525
82-83	0.6688538595700395
84-85	0.6580698164486259
86-87	0.6601033549065908
88-89	0.6699985111144198
90-91	0.6811703940448267
92-93	0.6729293222115691
94-95	0.6720257602781847
96-97	0.6699398110352208
98-99	0.6721200996911831
100-101	0.6615561818667657
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.3850053E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.68660621196306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.21636727299425	40.91798096691591
2	13.05494459929098	14.017513396422574
3	4.237847104997465	6.825468861375198
4	1.986253039625085	4.265407391026664
5	1.0987437648263016	2.9493911915039703
6	0.6854126243262809	2.2078486592947932
7	0.47643109545753637	1.7904578026274056
8	0.3372841294016416	1.448611218938458
9	0.26247145585940407	1.2682081523344024
>10	1.4777434524557673	14.679048450128796
>50	0.11292628835633607	4.178711368706662
>100	0.05237656109372173	4.798530523693268
>500	0.0010488217831552745	0.3538763796671979
>1k	1.3409839613514119E-4	0.10627945818992332
>5k	0.0	0.0
>10k+	1.5691135760081853E-5	0.19266617917481696
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	34568	0.14493888126789486	Illumina Single End PCR Primer 1 (100% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.1982469389061735	4.19286279992753E-6	0.0	0.0	0.0
2	0.198624296558167	2.9350039599492714E-5	0.0	2.5157176799565185E-5	0.0
3	0.1986746109117661	2.9350039599492714E-5	0.0	2.5157176799565185E-5	4.19286279992753E-6
4	0.19918614017335726	2.9350039599492714E-5	0.0	3.354290239942024E-5	4.19286279992753E-6
5	0.19925322597815612	2.9350039599492714E-5	0.0	3.773576519934778E-5	4.19286279992753E-6
6	0.1994460976669528	2.9350039599492714E-5	0.0	4.1928627999275306E-5	4.19286279992753E-6
7	0.19974379092574762	3.354290239942024E-5	0.0	4.6121490799202835E-5	4.19286279992753E-6
8	0.20084651384212857	4.1928627999275306E-5	0.0	5.031435359913037E-5	4.19286279992753E-6
9	0.2010477712565251	4.6121490799202835E-5	0.0	6.289294199891296E-5	4.19286279992753E-6
10-11	0.20171653287311353	5.45072163990579E-5	0.0	6.289294199891296E-5	4.19286279992753E-6
12-13	0.2024356088433011	5.660364779902166E-5	0.0	6.289294199891296E-5	8.38572559985506E-6
14-15	0.2031127561854894	7.127866759876802E-5	0.0	7.337509899873179E-5	8.38572559985506E-6
16-17	0.20370604627167915	7.966439319862308E-5	0.0	8.385725599855061E-5	8.38572559985506E-6
18-19	0.20452784738046495	7.966439319862308E-5	0.0	8.595368739851438E-5	1.0482156999818826E-5
20-21	0.2082385309584008	8.385725599855061E-5	0.0	1.027251385982245E-4	1.2578588399782592E-5
22-23	0.20900792128218748	9.014655019844192E-5	0.0	1.0482156999818826E-4	1.2578588399782592E-5
24-25	0.2097940830571739	1.0691800139815203E-4	0.0	1.1530372699800709E-4	1.2578588399782592E-5
26-27	0.2105802448321603	1.1320729559804332E-4	0.0	1.1740015839797085E-4	1.2578588399782592E-5
28-29	0.2114502638631453	1.1949658979793462E-4	0.0	1.1949658979793462E-4	1.4675019799746357E-5
30-31	0.21248380454332744	1.4255733519753604E-4	0.0	1.3207517819771721E-4	2.0964313999637653E-5
32-33	0.21354669526310907	1.509430607973911E-4	0.0	1.3626804099764472E-4	2.5157176799565185E-5
34-35	0.21458862166889103	1.635216491971737E-4	0.0	1.4884662939742736E-4	2.5157176799565185E-5
36-37	0.21585276980306922	1.8867882599673887E-4	0.0	1.635216491971737E-4	2.5157176799565185E-5
38-39	0.21742718978444198	1.9706455159659394E-4	0.0	1.6561808059713744E-4	2.5157176799565185E-5
40-41	0.21878987019441842	2.0125741439652148E-4	0.0	1.7819666899692005E-4	2.5157176799565185E-5
42-43	0.22026575789999292	2.1383600279630406E-4	0.0	1.928716887966664E-4	3.1446470999456474E-5
44-45	0.22194709588276385	2.2012529699619535E-4	0.0	1.9706455159659394E-4	3.354290239942024E-5
46-47	0.22413996312712597	2.41089610995833E-4	0.0	1.9706455159659394E-4	3.563933379938401E-5
48-49	0.22636218041108758	2.5157176799565185E-4	0.0	2.0335384579648523E-4	3.773576519934778E-5
50-51	0.22841249032025213	2.5576463079557936E-4	0.0	2.05450277196449E-4	3.773576519934778E-5
52-53	0.230559236073815	2.7463251339525325E-4	0.0	2.0964313999637652E-4	3.773576519934778E-5
54-55	0.23287159990797507	2.8721110179503583E-4	0.0	2.117395713963403E-4	3.9832196599311545E-5
56-57	0.23541247476473115	2.9978969019481847E-4	0.0	2.2431815979612288E-4	4.1928627999275306E-5
58-59	0.23932651218846349	3.144647099945648E-4	2.096431399963765E-6	2.3270388539597795E-4	4.402505939923907E-5
60-61	0.24271434533080494	3.186575727944923E-4	4.19286279992753E-6	2.599574935955069E-4	4.6121490799202835E-5
62-63	0.24725311931172647	3.249468669943836E-4	4.19286279992753E-6	2.7253608199528944E-4	4.6121490799202835E-5
64-65	0.25547113039958447	3.3542902399420245E-4	4.19286279992753E-6	2.8092180759514457E-4	5.031435359913037E-5
66-67	0.2604417692488985	3.5220047519391254E-4	4.19286279992753E-6	2.830182389951083E-4	5.031435359913037E-5
68-69	0.2650664969172186	3.6058620079376766E-4	4.19286279992753E-6	3.03982552994746E-4	5.241078499909414E-5
70-71	0.27095117985691686	3.7316478919355024E-4	4.19286279992753E-6	3.249468669943836E-4	5.45072163990579E-5
72-73	0.27808323947959357	3.8155051479340526E-4	4.19286279992753E-6	3.3752545539416626E-4	5.870007919898543E-5
74-75	0.28768070242862775	3.8155051479340526E-4	6.289294199891295E-6	3.5220047519391254E-4	5.870007919898543E-5
76-77	0.3002278443574109	3.8364694619336906E-4	1.2578588399782592E-5	3.605862007937676E-4	5.870007919898543E-5
78-79	0.31731795312991545	3.857433775933328E-4	1.2578588399782592E-5	3.7106835779358643E-4	6.498937339887673E-5
80-81	0.338405956582151	3.899362403932603E-4	1.677145119971012E-5	3.8155051479340526E-4	6.708580479884048E-5
82-83	0.3702486531162006	4.0251482879304296E-4	1.677145119971012E-5	3.8155051479340526E-4	6.708580479884048E-5
84-85	0.41262382100366823	4.0670769159297047E-4	1.677145119971012E-5	3.9622553459315164E-4	6.708580479884048E-5
86-87	0.4606404857884383	4.1509341719282554E-4	1.677145119971012E-5	4.2557557419264436E-4	6.708580479884048E-5
88-89	0.5095187838785935	4.2138271139271685E-4	1.677145119971012E-5	4.4863631959224577E-4	6.708580479884048E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	45150	0.0	47.213673	1
ATCGGAA	45005	0.0	46.66722	2
TCGGAAG	43950	0.0	45.02266	3
CGGAAGA	46020	0.0	37.181244	4
AAGAGCG	35470	0.0	36.57504	7
CGCCGTA	13440	0.0	34.478867	46-47
GATCTCG	32710	0.0	33.95491	34-35
CCGTATC	16230	0.0	32.640427	48-49
AGAGCGT	26560	0.0	32.30281	8
AGACGGC	11980	0.0	32.125053	9
GAGCGTC	26945	0.0	31.285955	9
ATCGGGA	7310	0.0	30.859787	2
GATCGGG	7740	0.0	30.357132	1
AGAGCGG	14015	0.0	30.271257	8
TAGATCT	27790	0.0	30.08033	32-33
GGCGCCG	21080	0.0	29.635807	44-45
TCGGGGG	31235	0.0	29.455574	38-39
GAGCGGC	14525	0.0	28.839905	9
AAGGGCG	9465	0.0	28.532228	7
GCCGGAT	14660	0.0	28.396667	46-47
>>END_MODULE