FastQCFastQC Report
Wed 25 May 2016
SRR1033292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23850053
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC4865442.0400122381279404TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG434860.18233083171764858TruSeq Adapter, Index 2 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA767400.066.352231
ATCGGAA776250.065.27272
TCGGAAG772800.065.2382353
CGGAAGA802800.062.918744
AGAGCAC839100.060.5036358
GAGCACA854450.059.5222479
AAGAGCA935900.054.2708857
GAAGAGC983050.051.768426
GGAAGAG1099100.046.2721445
CTCGTAT625200.043.11053542-43
CGTATGC637200.042.93879344-45
TATGCCG643950.042.5210746-47
ATCTCGT649600.041.2592540-41
ACCGATG669050.041.17176432-33
CGATGTA661400.041.1351334-35
TCGTATG631150.040.05612642-43
CCGTCTT680450.039.87274650-51
TATCTCG618550.039.6961338-39
TGCCGTC682900.039.69428648-49
ATGTATC674600.039.5332736-37
Produced by FastQC (version 0.11.5)