##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033292_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23850053
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50029016707007	33.0	33.0	33.0	33.0	33.0
2	32.595612890252276	33.0	33.0	33.0	33.0	33.0
3	32.54990611551261	33.0	33.0	33.0	33.0	33.0
4	36.318341305153496	37.0	37.0	37.0	37.0	37.0
5	36.427280979207886	37.0	37.0	37.0	37.0	37.0
6	36.29736478992311	37.0	37.0	37.0	37.0	37.0
7	36.25631070924664	37.0	37.0	37.0	33.0	37.0
8	36.27478509167254	37.0	37.0	37.0	37.0	37.0
9	36.286357812286624	37.0	37.0	37.0	37.0	37.0
10-11	36.30396896811927	37.0	37.0	37.0	37.0	37.0
12-13	36.24791395641762	37.0	37.0	37.0	35.0	37.0
14-15	38.04020259409906	40.0	37.0	40.0	33.0	40.0
16-17	37.99098243094051	40.0	37.0	40.0	33.0	40.0
18-19	37.95178872348837	40.0	37.0	40.0	33.0	40.0
20-21	37.973204566882934	40.0	37.0	40.0	33.0	40.0
22-23	37.906358216478594	40.0	37.0	40.0	33.0	40.0
24-25	37.246498152435976	40.0	37.0	40.0	33.0	40.0
26-27	36.22013349404297	37.0	37.0	40.0	30.0	40.0
28-29	36.01966731478542	37.0	37.0	40.0	30.0	40.0
30-31	35.84476084812054	37.0	37.0	40.0	27.0	40.0
32-33	35.72342141126479	37.0	37.0	40.0	27.0	40.0
34-35	35.82004478145185	37.0	37.0	40.0	27.0	40.0
36-37	35.796315022863894	37.0	37.0	40.0	27.0	40.0
38-39	35.678970713398414	37.0	37.0	40.0	27.0	40.0
40-41	35.5635417875172	37.0	37.0	40.0	27.0	40.0
42-43	35.36936486053092	37.0	35.0	40.0	27.0	40.0
44-45	35.16127551163094	37.0	33.0	40.0	27.0	40.0
46-47	35.041368440564895	37.0	33.0	40.0	27.0	40.0
48-49	34.91298669231469	37.0	33.0	40.0	27.0	40.0
50-51	34.823073684574204	37.0	33.0	40.0	27.0	40.0
52-53	34.67357334593764	37.0	33.0	40.0	27.0	40.0
54-55	34.51411881558502	37.0	33.0	40.0	27.0	40.0
56-57	34.36020045741617	37.0	33.0	40.0	27.0	40.0
58-59	34.12025537217883	37.0	33.0	40.0	27.0	40.0
60-61	33.88456514121793	37.0	33.0	40.0	24.5	40.0
62-63	33.67736155554874	37.0	33.0	37.0	22.0	40.0
64-65	33.34052674851499	37.0	33.0	37.0	22.0	40.0
66-67	33.08377998153715	37.0	33.0	37.0	22.0	40.0
68-69	32.7567859492807	37.0	33.0	37.0	22.0	40.0
70-71	32.425789787553086	37.0	33.0	37.0	22.0	40.0
72-73	32.041623387587435	37.0	33.0	37.0	22.0	38.5
74-75	31.54159141700859	37.0	33.0	37.0	15.0	37.0
76-77	31.321183709738506	37.0	33.0	37.0	15.0	37.0
78-79	31.091692982820625	35.0	33.0	37.0	15.0	37.0
80-81	30.86417000834338	33.0	33.0	37.0	15.0	37.0
82-83	30.667077050101312	33.0	33.0	37.0	15.0	37.0
84-85	30.49864694221015	33.0	30.0	37.0	15.0	37.0
86-87	30.30883625290057	33.0	27.0	37.0	10.5	37.0
88-89	30.098638250405564	33.0	27.0	37.0	6.0	37.0
90-91	29.870197835619066	33.0	27.0	37.0	4.0	37.0
92-93	29.619530237521907	33.0	27.0	37.0	2.0	37.0
94-95	29.38574583041807	33.0	27.0	37.0	2.0	37.0
96-97	29.23780683003094	33.0	27.0	37.0	2.0	37.0
98-99	28.945774795552865	33.0	27.0	37.0	2.0	37.0
100-101	26.52090337073884	30.0	21.0	35.0	2.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18905116932874222
1101	2	0.1329543987703019
1101	3	0.2252242290532962
1101	4	0.33728177508294266
1101	5	0.2374970612491154
1101	6	0.27797586484647496
1101	7	0.3070814163075397
1101	8	0.2975996045508964
1101	9	0.29721419937678917
1101	10-11	0.2836295294485325
1101	12-13	0.303046110704301
1101	14-15	0.6950034819338384
1101	16-17	0.7247440876862257
1101	18-19	0.7143176435350469
1101	20-21	0.6812480894752113
1101	22-23	0.6980401755340466
1101	24-25	0.808051310830237
1101	26-27	0.9632167201523956
1101	28-29	1.0134672818305148
1101	30-31	1.0515117545802255
1101	32-33	1.130527616880535
1101	34-35	0.9958933845921578
1101	36-37	0.9917943378171827
1101	38-39	0.9937978131472107
1101	40-41	1.008281002876977
1101	42-43	1.0442031241171819
1101	44-45	1.2163080659401047
1101	46-47	1.1201225242080142
1101	48-49	1.1030424458889385
1101	50-51	1.0897611102104108
1101	52-53	1.0161033088437463
1101	54-55	1.0751510951220382
1101	56-57	1.075792719449339
1101	58-59	1.048018073458124
1101	60-61	1.07430682592274
1101	62-63	1.0548959292765048
1101	64-65	1.0929816848040588
1101	66-67	1.1144150440179459
1101	68-69	1.1369360546410263
1101	70-71	1.1665805448539714
1101	72-73	1.1422838679098177
1101	74-75	1.0826818167721726
1101	76-77	1.078300848011775
1101	78-79	1.057778702504578
1101	80-81	1.100638942650555
1101	82-83	1.1244261378812688
1101	84-85	1.0974503429708555
1101	86-87	1.1646580919882652
1101	88-89	1.239249197605968
1101	90-91	1.2717492376823785
1101	92-93	1.3221480258012228
1101	94-95	1.3359136449165518
1101	96-97	1.4590331109253434
1101	98-99	1.4955039931579712
1101	100-101	1.6498030187762858
1102	1	0.1481462889740186
1102	2	0.1324320789349187
1102	3	0.214501503765959
1102	4	0.3077293515839301
1102	5	0.1941275972920522
1102	6	0.22240096153541344
1102	7	0.24281097279593666
1102	8	0.22015201354295044
1102	9	0.2122260352186487
1102	10-11	0.24285413214261098
1102	12-13	0.27306458561513125
1102	14-15	0.6144579292646384
1102	16-17	0.6367510813493524
1102	18-19	0.6276417253629063
1102	20-21	0.5908499679911756
1102	22-23	0.5942445064304991
1102	24-25	0.7048167396615952
1102	26-27	0.8447908062717033
1102	28-29	0.8354359911732416
1102	30-31	0.8719518990594395
1102	32-33	0.9961079331259768
1102	34-35	0.895818195176723
1102	36-37	0.8533381281613757
1102	38-39	0.8577255722568964
1102	40-41	0.8883998799113755
1102	42-43	0.9117797466480582
1102	44-45	1.072820264862834
1102	46-47	0.9381581602865623
1102	48-49	0.9032824459417057
1102	50-51	0.8979240354661329
1102	52-53	0.8772658476951491
1102	54-55	0.9043831679001784
1102	56-57	0.895694330693523
1102	58-59	0.8146737114232252
1102	60-61	0.8433455875881535
1102	62-63	0.8649199177566018
1102	64-65	0.9261445700394191
1102	66-67	0.9213968996718762
1102	68-69	0.9822334860781652
1102	70-71	1.0004084955155292
1102	72-73	1.0034873872688834
1102	74-75	0.9296102935414225
1102	76-77	0.9592393755635662
1102	78-79	0.9735530142796449
1102	80-81	0.968264012578409
1102	82-83	1.0123203899372974
1102	84-85	1.0279267296327035
1102	86-87	1.0787643189845646
1102	88-89	1.0696903057171063
1102	90-91	1.1262778002855178
1102	92-93	1.1313313754025742
1102	94-95	1.1921581746010474
1102	96-97	1.234413526284559
1102	98-99	1.3008833098294126
1102	100-101	1.4679456045501666
1103	1	0.14096226240898346
1103	2	0.11036873914780188
1103	3	0.12804289019771886
1103	4	0.256849371483554
1103	5	0.1569269823447783
1103	6	0.1664983454400044
1103	7	0.15860071135666942
1103	8	0.1690061196391639
1103	9	0.17722005699570786
1103	10-11	0.21324392666018355
1103	12-13	0.20083202668186573
1103	14-15	0.49148945102069064
1103	16-17	0.4863020084472751
1103	18-19	0.4677540049959177
1103	20-21	0.4421211486228884
1103	22-23	0.48448767449758634
1103	24-25	0.5882433009311825
1103	26-27	0.7585948133864662
1103	28-29	0.8039433831413376
1103	30-31	0.803927770368766
1103	32-33	0.8617086383868937
1103	34-35	0.7520446628299737
1103	36-37	0.7013604515065026
1103	38-39	0.7016959427499927
1103	40-41	0.7640300403579801
1103	42-43	0.8013494262399661
1103	44-45	0.9273194152768198
1103	46-47	0.864089044230191
1103	48-49	0.7737637042958738
1103	50-51	0.7750143307287516
1103	52-53	0.8561851805793026
1103	54-55	0.839829494271811
1103	56-57	0.7976707317996556
1103	58-59	0.7395543910509161
1103	60-61	0.7480353372348887
1103	62-63	0.7209880876065
1103	64-65	0.6678568284714999
1103	66-67	0.6432890921870751
1103	68-69	0.6181947579643108
1103	70-71	0.6255289662123431
1103	72-73	0.6298332691370447
1103	74-75	0.611519393496117
1103	76-77	0.552609911230217
1103	78-79	0.5991703379816578
1103	80-81	0.6109599928442151
1103	82-83	0.5754118085041888
1103	84-85	0.5749045044134995
1103	86-87	0.7003271185802546
1103	88-89	0.6021259505541359
1103	90-91	0.6189711270320046
1103	92-93	0.6450035710269084
1103	94-95	0.6650449190831687
1103	96-97	0.6596788320449001
1103	98-99	0.7198274281436561
1103	100-101	0.9807657386550517
1104	1	0.10949382086556625
1104	2	0.0526727779438545
1104	3	-0.002095478493735925
1104	4	0.21846105880496225
1104	5	0.1291066623364827
1104	6	0.1419934674676
1104	7	0.13303477052891566
1104	8	0.019573179752299552
1104	9	0.10876734402399535
1104	10-11	0.12103542650585553
1104	12-13	0.11642755524273696
1104	14-15	0.3363678152937055
1104	16-17	0.27087807324401325
1104	18-19	0.33375913593915385
1104	20-21	0.40472330289720304
1104	22-23	0.3963683679532082
1104	24-25	0.39998115570548975
1104	26-27	0.5339471916729721
1104	28-29	0.591768771960588
1104	30-31	0.67157760355893
1104	32-33	0.6154200901566327
1104	34-35	0.6247208530073536
1104	36-37	0.556529884525574
1104	38-39	0.6289642853068926
1104	40-41	0.6131184638681759
1104	42-43	0.6298049329459943
1104	44-45	0.6477999313497307
1104	46-47	0.698247246097921
1104	48-49	0.6466686829587331
1104	50-51	0.74219485124042
1104	52-53	0.5535419546677431
1104	54-55	0.6914472809665568
1104	56-57	0.6769862746665467
1104	58-59	0.6088025241443233
1104	60-61	0.5259370547759659
1104	62-63	0.5334386867653862
1104	64-65	0.5940693809684205
1104	66-67	0.5797157856877817
1104	68-69	0.6860716497843029
1104	70-71	0.7819623318923803
1104	72-73	0.7095665110925253
1104	74-75	0.7559373592545953
1104	76-77	0.6746469537590549
1104	78-79	0.6761472847097352
1104	80-81	0.6711882339656903
1104	82-83	0.7304874106841481
1104	84-85	0.703751131681269
1104	86-87	0.7564718226350351
1104	88-89	0.8036624653063136
1104	90-91	0.8014048336346455
1104	92-93	0.8271660164782375
1104	94-95	0.8429540177902233
1104	96-97	0.8510686681701358
1104	98-99	0.8718531567636809
1104	100-101	0.9660606760904571
1105	1	0.08473572978453348
1105	2	0.02788889695850827
1105	3	9.940156029202285E-4
1105	4	0.2068252903189105
1105	5	0.12648538915902208
1105	6	0.05897821193295272
1105	7	-0.007451990919292939
1105	8	0.04561346826687185
1105	9	0.0891630075721963
1105	10-11	0.128591781563685
1105	12-13	0.08065718848223469
1105	14-15	0.12256222335926736
1105	16-17	0.11342213046543748
1105	18-19	0.11083931804917313
1105	20-21	0.31499636675338394
1105	22-23	0.3496755560488509
1105	24-25	0.3374353477996834
1105	26-27	0.37208362825088415
1105	28-29	0.38753201791993064
1105	30-31	0.41106812207327437
1105	32-33	0.5433885879109113
1105	34-35	0.460392604964305
1105	36-37	0.39722379682747544
1105	38-39	0.38967389192923463
1105	40-41	0.23096906830237174
1105	42-43	0.40318082420755985
1105	44-45	0.5331353503620093
1105	46-47	0.4112889300336846
1105	48-49	0.41125002385589227
1105	50-51	0.37737471071370976
1105	52-53	0.4192835461001252
1105	54-55	0.4119827779733072
1105	56-57	0.4420947124418575
1105	58-59	0.367085949026837
1105	60-61	0.3544163493140715
1105	62-63	0.36349496614023025
1105	64-65	0.33768069353222074
1105	66-67	0.40506753941049567
1105	68-69	0.4940359122787825
1105	70-71	0.4358071906942911
1105	72-73	0.39770692689175036
1105	74-75	0.3448758337852844
1105	76-77	0.3308071456133561
1105	78-79	0.508103790168299
1105	80-81	0.4747153031917435
1105	82-83	0.4966703422586072
1105	84-85	0.5850150527846232
1105	86-87	0.5473494872707718
1105	88-89	0.5900498635247509
1105	90-91	0.6181881132004676
1105	92-93	0.6522536582911549
1105	94-95	0.546706650102287
1105	96-97	0.5271729823825915
1105	98-99	0.5980963270557389
1105	100-101	0.780168909581711
1106	1	-0.02035039351250134
1106	2	-0.053012318352948284
1106	3	-0.08159562512447849
1106	4	0.10248870579336256
1106	5	-0.18536132447068354
1106	6	-0.09163859549580167
1106	7	0.009811818938239014
1106	8	-0.08302760284600197
1106	9	-0.043342235646242955
1106	10-11	-0.19921481693020837
1106	12-13	-0.01818317064461894
1106	14-15	-0.2956125314887572
1106	16-17	-0.2022206945876306
1106	18-19	-0.02410611844305066
1106	20-21	0.056743508340517224
1106	22-23	0.054864900753585744
1106	24-25	-0.09902395741153214
1106	26-27	-0.06470205141044261
1106	28-29	-0.3144623671360236
1106	30-31	-0.305903878882269
1106	32-33	-0.1367084748819778
1106	34-35	-0.21138755561499778
1106	36-37	0.03303050928764861
1106	38-39	-0.13950476918329002
1106	40-41	-0.6016716943140068
1106	42-43	-0.025958885601149007
1106	44-45	0.2313998629361791
1106	46-47	0.15218210524565734
1106	48-49	-0.15334106847064533
1106	50-51	-0.3203694176918006
1106	52-53	-0.0703791168709742
1106	54-55	-0.16928133712195859
1106	56-57	-0.0700021770933148
1106	58-59	0.09614799384627304
1106	60-61	0.11235095756108393
1106	62-63	-0.11488048613799862
1106	64-65	-0.8932561147438136
1106	66-67	-0.5149791161588695
1106	68-69	-0.1419594691448296
1106	70-71	-0.5677999462563328
1106	72-73	-0.5876789793262134
1106	74-75	-0.295925910414816
1106	76-77	-0.3223036134102344
1106	78-79	-0.19846739571559624
1106	80-81	-0.2204975109634688
1106	82-83	-0.29231744162402507
1106	84-85	-0.43234585245345514
1106	86-87	-0.24342810585366692
1106	88-89	-0.32950812426700793
1106	90-91	-0.992036540684655
1106	92-93	-1.0952353607995136
1106	94-95	-1.618412400760441
1106	96-97	-1.1761785994359215
1106	98-99	-1.0279859226549775
1106	100-101	-0.8966158893076255
1107	1	0.08776195307434875
1107	2	0.045433753617096784
1107	3	0.08081807662269824
1107	4	0.21202614195904346
1107	5	0.13219386673895883
1107	6	0.11934824216277917
1107	7	0.15169331242895367
1107	8	0.12816324692264658
1107	9	0.11856471358265708
1107	10-11	0.10093757616010635
1107	12-13	0.09494869328914035
1107	14-15	0.14685568613712263
1107	16-17	0.05861181158302742
1107	18-19	0.2393139789990073
1107	20-21	0.1804613402648343
1107	22-23	0.23424893450589224
1107	24-25	0.3123566010142582
1107	26-27	0.3210818535373221
1107	28-29	0.3534800627940058
1107	30-31	0.39007809413297423
1107	32-33	0.48346420865376416
1107	34-35	0.3826495377425232
1107	36-37	0.28940371197487025
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2216	60-61	-1.2774144917603465
2216	62-63	-1.370981477739491
2216	64-65	-1.3361692972325727
2216	66-67	-1.2580754206119664
2216	68-69	-1.3166433466112473
2216	70-71	-1.3335978142345901
2216	72-73	-1.422390789587574
2216	74-75	-1.2410748848622468
2216	76-77	-1.3087665215281028
2216	78-79	-1.2646109110440946
2216	80-81	-1.2920942429813849
2216	82-83	-1.2858368824952748
2216	84-85	-1.3056061515732829
2216	86-87	-1.3086467774937205
2216	88-89	-1.3937595038126673
2216	90-91	-1.4087728849437404
2216	92-93	-1.374886027428552
2216	94-95	-1.4344843595490602
2216	96-97	-1.528253173725524
2216	98-99	-1.6637885218790203
2216	100-101	-1.549066900191253
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7893.0
3	7440.0
4	4493.0
5	8257.0
6	12964.0
7	22476.0
8	38704.0
9	57305.0
10	65211.0
11	67225.0
12	71390.0
13	76484.0
14	79631.0
15	84470.0
16	89696.0
17	96720.0
18	106416.0
19	118191.0
20	133208.0
21	154487.0
22	182111.0
23	220395.0
24	268296.0
25	326702.0
26	400547.0
27	510963.0
28	541092.0
29	561884.0
30	671548.0
31	808751.0
32	990468.0
33	1229006.0
34	1561865.0
35	2045273.0
36	2933672.0
37	5193296.0
38	4094176.0
39	7347.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.800092238432825	12.655677520480607	19.717495892638357	40.82673434844821
2	21.804893403118935	22.82491808359706	31.49285110173039	23.87733741155362
3	25.716748739040042	23.624067789006112	27.517185093345315	23.141998378608534
4	25.798395356046992	29.165343467550098	19.233949682067433	25.802311494335477
5	29.135803328414546	30.839473855676687	20.525077658170215	19.49964515773856
6	23.74751056652882	32.903417382889224	19.985587694588453	23.363484355993506
7	19.491915573181977	17.33661628913849	39.396027312644854	23.77544082503468
8	21.484484751459462	23.135697015012923	25.036644572655664	30.343173660871948
9	22.84704781250363	20.534580220932668	28.58628997507787	28.03208199148583
10-11	26.50252944039605	29.503341961553158	19.57764245844747	24.416486139603325
12-13	22.952296729670987	23.545507686226745	27.023645650111618	26.478549933990653
14-15	23.229982908367468	25.47340040132957	26.028197640211314	25.268419050091644
16-17	24.99271436073974	24.540362168965153	24.9629743194345	25.503949150860606
18-19	23.822716952090566	24.93773965487078	26.031299092528204	25.20824430051045
20-21	24.882861890615583	24.962981212787295	26.135043646865004	24.01911324973212
22-23	24.031784504262767	27.430974514329627	24.8281057026737	23.70913527873391
24-25	23.795789887762513	25.249231102337593	25.983904522140893	24.971074487759
26-27	23.769956814711772	25.098735720304454	24.871317618902836	26.259989846080934
28-29	24.938950889960314	26.08766625784858	24.851764831578173	24.121618020612935
30-31	23.70458712572795	25.22086222253567	26.230931700858513	24.84361895087787
32-33	23.677130981284673	26.433529196746836	25.028532441225483	24.860807380743008
34-35	25.02547638080307	24.99159523163919	24.829412292680548	25.153516094877197
36-37	23.824238099982324	26.147179432733314	26.087410564740743	23.94117190254362
38-39	24.961716159259854	25.07925376107223	26.149956009091813	23.809074070576106
40-41	23.849261641441153	26.25610494431857	26.14558842634864	23.749044987891633
42-43	23.617525475057306	25.096916628729044	26.390315876404024	24.89524201980963
44-45	24.889537134444375	25.009425514794348	25.171882637411336	24.929154713349945
46-47	23.938475461136623	26.025345313567698	26.164088665107695	23.872090560187985
48-49	24.806847481079135	25.063103853625435	26.319246236449125	23.810802428846305
50-51	23.87578752788374	25.076392673330773	24.995177042276783	26.05264275650871
52-53	24.985091334327727	25.120759405761607	26.050802275421404	23.843346984489262
54-55	23.560549707417458	25.033328300457725	26.41524469062721	24.99087730149761
56-57	23.689817504661114	24.983871054980803	26.47508620447842	24.851225235879664
58-59	24.966022088085086	24.93698651319559	26.184887304024016	23.9121040946953
60-61	23.6519390543912	25.20522700725235	26.237228906786914	24.90560503156953
62-63	24.704561453175806	25.324398650183294	26.394673001355596	23.576366895285304
64-65	23.811284612239646	27.506628601621973	25.02720014919883	23.654886636939548
66-67	23.626693827472835	27.57215466145924	25.149782686017513	23.651368825050408
68-69	23.777079237517835	27.55596601818872	25.074514509464613	23.59244023482883
70-71	23.73533090261896	27.55520291715914	25.008221155734955	23.701245024486948
72-73	23.61433536437005	27.674875607194664	25.12515171349934	23.585637314935944
74-75	24.018030484041272	27.01255003500411	25.244006795288882	23.72541268566573
76-77	24.252640025579815	26.280931115750562	25.331612470630567	24.134816388039056
78-79	24.118682251984932	25.99712881141187	25.594869747249614	24.289319189353584
80-81	24.32188010315281	26.088439354095073	25.419662363056062	24.170018179696058
82-83	24.368939034960423	25.975224956114523	25.499934449846144	24.155901559078906
84-85	24.32670588584562	26.1245169236281	25.610515780732428	23.93826140979385
86-87	24.37324997170687	26.181693783138034	25.50868830854782	23.93636793660728
88-89	24.408926721380755	25.845764941769726	25.42319802587123	24.32211031097829
90-91	24.259908184221402	25.961634446074022	25.375621296951493	24.40283607275308
92-93	24.446301186706993	26.15057502697919	25.41564603971721	23.987477746596607
94-95	24.522173584634743	25.99162500106935	25.378829560015653	24.107371854280252
96-97	24.37309054629746	25.996450771366934	25.49024656096275	24.14021212137286
98-99	24.615398485168058	25.8911242320696	25.385691744321058	24.10778553844128
100-101	24.802463737967464	25.92339331268128	25.065259770851018	24.208883178500237
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1582.0
1	982.5
2	355.5
3	327.5
4	295.0
5	268.5
6	252.0
7	247.0
8	267.5
9	282.5
10	307.5
11	370.0
12	440.0
13	535.0
14	693.5
15	915.5
16	1356.0
17	2165.0
18	3451.0
19	5547.5
20	9032.5
21	14482.0
22	22861.5
23	34719.0
24	50308.0
25	70031.5
26	94334.0
27	122357.5
28	152006.0
29	181648.0
30	212565.0
31	246332.5
32	280322.0
33	313243.5
34	346953.0
35	386376.0
36	430005.0
37	474138.5
38	515160.5
39	554407.5
40	593106.5
41	630278.0
42	672312.0
43	722498.5
44	756244.5
45	780259.0
46	806943.5
47	817640.5
48	830515.0
49	832806.5
50	825659.0
51	818483.5
52	800784.5
53	787410.0
54	765507.5
55	744203.5
56	719872.5
57	682699.5
58	655317.5
59	632736.5
60	603817.0
61	567482.0
62	525174.5
63	486114.5
64	447288.5
65	397075.5
66	337819.0
67	285987.5
68	239114.0
69	185616.5
70	134352.0
71	91577.0
72	59557.5
73	36426.5
74	20932.5
75	11381.0
76	6041.5
77	3231.5
78	1812.0
79	1017.0
80	602.5
81	388.5
82	254.5
83	168.0
84	126.0
85	92.0
86	60.0
87	32.5
88	22.0
89	20.5
90	16.0
91	13.5
92	12.5
93	5.5
94	3.0
95	3.5
96	1.5
97	0.5
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0314338924110567
2	0.0018197024551685481
3	7.044009503878252E-4
4	1.1320729559804332E-4
5	7.966439319862308E-5
6	0.015781935578927226
7	4.192862799927531E-6
8	0.0
9	6.792437735882599E-4
10-11	8.909833449846003E-4
12-13	9.014655019844192E-5
14-15	3.291397297943112E-4
16-17	2.2012529699619538E-4
18-19	2.0964313999637656E-6
20-21	4.192862799927531E-6
22-23	1.4675019799746358E-5
24-25	0.0
26-27	6.226401257892383E-4
28-29	0.006834366363881875
30-31	0.0019119454367669537
32-33	0.21127626005694827
34-35	0.008316543363656257
36-37	0.006360572867490063
38-39	9.99997777782716E-4
40-41	9.412976985837306E-4
42-43	0.06237302701172195
44-45	0.4054854720867916
46-47	0.11895571049674396
48-49	0.1714419670262368
50-51	0.002431860423957968
52-53	0.0028029287817515542
54-55	8.469582855853611E-4
56-57	3.102718471946373E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.007314449154473577
82-83	0.0079727286140622
84-85	0.007786146219465425
86-87	0.006331222827890571
88-89	0.013329110840969619
90-91	0.012840642324778061
92-93	0.020226370146850407
94-95	0.016104786014521644
96-97	0.007431849312871548
98-99	0.007811303396264989
100-101	0.007966439319862307
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.3850053E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.38356751640244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.60430614506083	35.128553483511034
2	14.820876939732432	14.341738001316722
3	5.017596067257606	7.2830759427058105
4	2.2745905044302686	4.402112129730791
5	1.2879698722479773	3.115828863650111
6	0.8083333447929673	2.346603057812996
7	0.5481109470195364	1.8563694108119315
8	0.4043320940125533	1.56504233357638
9	0.30492635246283284	1.327808228572422
>10	1.7201416920425983	15.568670013810653
>50	0.14232082367661378	4.744054612365525
>100	0.06463205204201589	5.415128226569537
>500	0.001654175567949677	0.5248592699817858
>1k	1.916340525423781E-4	0.15475681294314694
>5k	0.0	0.0
>10k+	1.735560129568268E-5	2.2253996126412363
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	486544	2.0400122381279404	TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG	43486	0.18233083171764858	TruSeq Adapter, Index 2 (100% over 49bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.2073244868680166	1.2578588399782592E-5	0.0	0.0	0.0
2	0.2077186159712098	4.1928627999275306E-5	0.0	8.38572559985506E-6	0.0
3	0.2077521588736092	4.6121490799202835E-5	0.0	8.38572559985506E-6	0.0
4	0.2081588665652022	4.6121490799202835E-5	0.0	1.2578588399782592E-5	0.0
5	0.2082217595072011	4.6121490799202835E-5	0.0	1.677145119971012E-5	0.0
6	0.2083768954307984	4.6121490799202835E-5	0.0	2.5157176799565185E-5	4.19286279992753E-6
7	0.2086787815523932	5.45072163990579E-5	0.0	2.5157176799565185E-5	4.19286279992753E-6
8	0.2097773116059742	6.289294199891296E-5	0.0	2.9350039599492714E-5	8.38572559985506E-6
9	0.20997018329477088	6.708580479884048E-5	0.0	3.773576519934778E-5	8.38572559985506E-6
10-11	0.21072489859875781	7.547153039869555E-5	0.0	4.1928627999275306E-5	8.38572559985506E-6
12-13	0.21150896394234428	8.385725599855061E-5	0.0	6.289294199891296E-5	8.38572559985506E-6
14-15	0.21223852206953167	1.027251385982245E-4	0.0	6.498937339887673E-5	8.38572559985506E-6
16-17	0.21299952666771851	1.1949658979793462E-4	0.0	7.127866759876802E-5	8.38572559985506E-6
18-19	0.2140938638584996	1.2578588399782592E-4	0.0	7.337509899873179E-5	8.38572559985506E-6
20-21	0.21949217471340632	1.2997874679775346E-4	0.0	8.385725599855061E-5	8.38572559985506E-6
22-23	0.22038735092119083	1.2997874679775346E-4	0.0	8.385725599855061E-5	8.38572559985506E-6
24-25	0.22133493791397446	1.5303949219735486E-4	0.0	1.0482156999818828E-4	8.38572559985506E-6
26-27	0.2222405962787588	1.5723235499728237E-4	4.19286279992753E-6	1.1530372699800709E-4	8.38572559985506E-6
28-29	0.2232426904879415	1.6142521779720993E-4	4.19286279992753E-6	1.1740015839797085E-4	1.886788259967389E-5
30-31	0.2246871317225165	1.7190737479702876E-4	6.289294199891295E-6	1.2368945259786214E-4	2.0964313999637653E-5
32-33	0.2261525372710912	1.802931003968838E-4	8.38572559985506E-6	1.2578588399782592E-4	2.0964313999637653E-5
34-35	0.22743974615066892	1.802931003968838E-4	1.2578588399782592E-5	1.278823153977897E-4	2.0964313999637653E-5
36-37	0.22889257311084382	1.8867882599673887E-4	1.2578588399782592E-5	1.3207517819771721E-4	2.0964313999637653E-5
38-39	0.23074372203701182	1.8867882599673887E-4	1.2578588399782592E-5	1.3417160959768097E-4	2.0964313999637653E-5
40-41	0.23280241767177623	2.0125741439652148E-4	1.2578588399782592E-5	1.3417160959768097E-4	2.0964313999637653E-5
42-43	0.23439570553574868	2.159324341962678E-4	1.2578588399782592E-5	1.4884662939742736E-4	2.0964313999637653E-5
44-45	0.2362573366189165	2.3270388539597795E-4	1.2578588399782592E-5	1.5303949219735486E-4	2.5157176799565185E-5
46-47	0.23875628284767333	2.4318604239579678E-4	1.2578588399782592E-5	1.5513592359731862E-4	2.5157176799565185E-5
48-49	0.24104558593643377	2.4947533659568804E-4	1.2578588399782592E-5	1.5932878639724615E-4	2.5157176799565185E-5
50-51	0.24321748886679623	2.6834321919536194E-4	1.2578588399782592E-5	1.6142521779720993E-4	2.5157176799565185E-5
52-53	0.24551937054395645	2.830182389951083E-4	1.2578588399782592E-5	1.7190737479702876E-4	2.5157176799565185E-5
54-55	0.24815668124511087	3.081754157946735E-4	1.2578588399782592E-5	1.740038061969925E-4	2.725360819952895E-5
56-57	0.25099105649786185	3.1446470999456474E-4	1.4675019799746357E-5	1.865823945967751E-4	2.9350039599492714E-5
58-59	0.25445855403340195	3.207540041944561E-4	1.677145119971012E-5	2.0335384579648523E-4	2.9350039599492714E-5
60-61	0.25820068408233726	3.333325925942387E-4	1.677145119971012E-5	2.3689674819590546E-4	2.9350039599492714E-5
62-63	0.26377928803764084	3.4591118099402127E-4	1.677145119971012E-5	2.4108961099583302E-4	3.354290239942024E-5
64-65	0.27760106025760195	3.626826321937314E-4	1.677145119971012E-5	2.5786106219554317E-4	3.354290239942024E-5
66-67	0.28246478110551787	3.857433775933328E-4	1.677145119971012E-5	2.725360819952895E-4	3.354290239942024E-5
68-69	0.2871691731670366	3.920326717932241E-4	2.0964313999637653E-5	2.830182389951083E-4	3.354290239942024E-5
70-71	0.29283582724113866	4.10900554392898E-4	2.0964313999637653E-5	2.893075331949996E-4	3.773576519934778E-5
72-73	0.3001523728270122	4.339612997924994E-4	2.0964313999637653E-5	3.018861215947822E-4	3.773576519934778E-5
74-75	0.3103095829598366	4.3815416259242694E-4	2.0964313999637653E-5	3.03982552994746E-4	3.773576519934778E-5
76-77	0.32372255105680475	4.4444345679231826E-4	2.0964313999637653E-5	3.0607898439470973E-4	3.773576519934778E-5
78-79	0.3425296371458797	4.46539888192282E-4	2.0964313999637653E-5	3.1027184719463724E-4	3.773576519934778E-5
80-81	0.3662486620050698	4.528291823921733E-4	2.0964313999637653E-5	3.1027184719463724E-4	3.773576519934778E-5
82-83	0.40195927447205254	4.6121490799202835E-4	2.0964313999637653E-5	3.1656114139452855E-4	3.773576519934778E-5
84-85	0.4478375792288596	4.7169706499184717E-4	2.0964313999637653E-5	3.186575727944923E-4	3.773576519934778E-5
86-87	0.49836996169358616	4.8637208479159356E-4	2.0964313999637653E-5	3.3962188679413E-4	3.773576519934778E-5
88-89	0.5494558020479032	4.968542417914123E-4	2.0964313999637653E-5	3.5220047519391254E-4	4.1928627999275306E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	76740	0.0	66.35223	1
ATCGGAA	77625	0.0	65.2727	2
TCGGAAG	77280	0.0	65.238235	3
CGGAAGA	80280	0.0	62.91874	4
AGAGCAC	83910	0.0	60.503635	8
GAGCACA	85445	0.0	59.522247	9
AAGAGCA	93590	0.0	54.270885	7
GAAGAGC	98305	0.0	51.76842	6
GGAAGAG	109910	0.0	46.272144	5
CTCGTAT	62520	0.0	43.110535	42-43
CGTATGC	63720	0.0	42.938793	44-45
TATGCCG	64395	0.0	42.52107	46-47
ATCTCGT	64960	0.0	41.25925	40-41
ACCGATG	66905	0.0	41.171764	32-33
CGATGTA	66140	0.0	41.13513	34-35
TCGTATG	63115	0.0	40.056126	42-43
CCGTCTT	68045	0.0	39.872746	50-51
TATCTCG	61855	0.0	39.69613	38-39
TGCCGTC	68290	0.0	39.694286	48-49
ATGTATC	67460	0.0	39.53327	36-37
>>END_MODULE