FastQCFastQC Report
Wed 25 May 2016
SRR1033291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22754866
Sequences flagged as poor quality0
Sequence length101
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA367050.034.8462872
GATCGGA374500.034.5080641
TCGGAAG357650.033.9377483
CGCCGTA84200.030.36880146-47
CGGAAGA365950.029.6909944
AAGAGCG264450.028.2805067
GATCTCG216450.027.59347334-35
CCGTATC107400.027.01970348-49
TGATACG26450.025.7526173
ATACGGC24300.025.1113975
GCCGGAT110600.024.86188146-47
GGCGCCG141050.024.8080444-45
AGAGCGT204500.024.2422318
ATCGGGA63250.023.7068462
TCGGGGG205550.023.67422138-39
GATCGGG65750.023.4452361
TAGATCT195800.023.4320232-33
GAGCGTC205000.023.1133489
AGAGCGG108550.023.053168
TACGGCG28200.022.6455366
Produced by FastQC (version 0.11.5)