##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033291_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22754866
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.334078038517124	33.0	33.0	33.0	27.0	33.0
2	31.256917047984373	33.0	33.0	33.0	27.0	33.0
3	31.360410560097343	33.0	33.0	33.0	27.0	33.0
4	35.028556573350066	37.0	37.0	37.0	33.0	37.0
5	35.06749896923146	37.0	37.0	37.0	33.0	37.0
6	35.02698904049797	37.0	37.0	37.0	33.0	37.0
7	34.91597594993528	37.0	37.0	37.0	33.0	37.0
8	34.93961533326542	37.0	37.0	37.0	33.0	37.0
9	34.83341809176112	37.0	37.0	37.0	33.0	37.0
10-11	34.89393552130784	37.0	37.0	37.0	33.0	37.0
12-13	34.81382560108243	37.0	37.0	37.0	33.0	37.0
14-15	36.29040577518673	40.0	37.0	40.0	33.0	40.0
16-17	36.199927149647905	40.0	37.0	40.0	33.0	40.0
18-19	36.197929489015664	40.0	37.0	40.0	33.0	40.0
20-21	36.093234365783566	40.0	37.0	40.0	33.0	40.0
22-23	36.00341272060226	40.0	37.0	40.0	33.0	40.0
24-25	35.20140070260137	37.0	35.0	40.0	24.5	40.0
26-27	34.25400953800387	37.0	33.0	40.0	22.0	40.0
28-29	33.85454007947135	37.0	33.0	40.0	18.5	40.0
30-31	33.795133269516946	37.0	33.0	40.0	15.0	40.0
32-33	33.61704129569473	37.0	33.0	40.0	15.0	40.0
34-35	33.47376899516789	37.0	33.0	40.0	15.0	40.0
36-37	33.355559290043715	37.0	33.0	40.0	15.0	40.0
38-39	33.11529171386903	37.0	33.0	40.0	15.0	40.0
40-41	32.79060922617606	37.0	33.0	40.0	15.0	40.0
42-43	32.473048380069564	37.0	33.0	40.0	10.5	40.0
44-45	32.3565594277725	37.0	33.0	40.0	6.0	40.0
46-47	32.188152327506565	37.0	33.0	40.0	6.0	40.0
48-49	32.0339528696851	37.0	33.0	40.0	6.0	40.0
50-51	31.779195425716857	37.0	33.0	40.0	6.0	40.0
52-53	31.570062442907815	37.0	33.0	40.0	6.0	40.0
54-55	31.413765345838556	37.0	33.0	40.0	6.0	40.0
56-57	31.217873684687923	37.0	27.0	38.5	6.0	40.0
58-59	30.990425586333927	37.0	27.0	37.0	6.0	40.0
60-61	30.755004204375453	37.0	27.0	37.0	2.0	40.0
62-63	30.477252008427563	37.0	27.0	37.0	2.0	40.0
64-65	30.203361733705663	37.0	27.0	37.0	2.0	40.0
66-67	29.926587262698007	35.0	27.0	37.0	2.0	40.0
68-69	29.917582002021017	33.0	27.0	37.0	2.0	40.0
70-71	29.852605570166837	33.0	27.0	37.0	2.0	40.0
72-73	29.671861767940097	33.0	27.0	37.0	2.0	37.0
74-75	29.424602873952324	33.0	27.0	37.0	2.0	37.0
76-77	29.191643866415212	33.0	27.0	37.0	2.0	37.0
78-79	28.948498927657937	33.0	27.0	37.0	2.0	37.0
80-81	28.730455147483617	33.0	27.0	37.0	2.0	37.0
82-83	28.49941329032656	33.0	27.0	37.0	2.0	37.0
84-85	28.316205927119064	33.0	27.0	37.0	2.0	37.0
86-87	28.106012116265596	33.0	27.0	37.0	2.0	37.0
88-89	27.860378786673586	33.0	27.0	37.0	2.0	37.0
90-91	27.639112596839723	33.0	27.0	37.0	2.0	37.0
92-93	27.400775442931632	33.0	27.0	37.0	2.0	37.0
94-95	27.149568030855466	33.0	27.0	37.0	2.0	37.0
96-97	26.805528980043213	33.0	22.0	37.0	2.0	37.0
98-99	26.359240942135187	33.0	22.0	37.0	2.0	37.0
100-101	23.406682575058888	30.0	8.5	35.0	2.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5380003161354558
1101	2	0.5837138742614449
1101	3	0.561533125781235
1101	4	0.6428644331842435
1101	5	0.6349853608642135
1101	6	0.6755876884524028
1101	7	0.7219994384856534
1101	8	0.7403263912794245
1101	9	0.7898686739857723
1101	10-11	0.7594604416996091
1101	12-13	0.7305251535265924
1101	14-15	1.1453678216067544
1101	16-17	1.203101580726404
1101	18-19	1.1481453954462992
1101	20-21	1.1711355663908165
1101	22-23	1.2173158870283274
1101	24-25	1.3712915142527677
1101	26-27	1.471175531888285
1101	28-29	1.6142849191236905
1101	30-31	1.4656961422724777
1101	32-33	1.6446364179620048
1101	34-35	1.6512799321677392
1101	36-37	1.5837763071850048
1101	38-39	1.6670715296987737
1101	40-41	1.6851635818408752
1101	42-43	1.7524664914007957
1101	44-45	1.6748170283490964
1101	46-47	1.680892205412249
1101	48-49	1.6586839970341813
1101	50-51	1.77436608298963
1101	52-53	1.7650081085450466
1101	54-55	1.7684086768512763
1101	56-57	1.723744625315021
1101	58-59	1.7668227232861256
1101	60-61	1.7135839436394278
1101	62-63	1.7664725513965642
1101	64-65	1.8067239367187184
1101	66-67	1.7578506853187825
1101	68-69	1.794934764566218
1101	70-71	1.8360229083982702
1101	72-73	1.835679530602004
1101	74-75	1.840544360546918
1101	76-77	1.8622341945649339
1101	78-79	1.8479369231998604
1101	80-81	1.8657154001672218
1101	82-83	1.8944934927320034
1101	84-85	1.9221125730170385
1101	86-87	1.9433303276092602
1101	88-89	2.000804510723885
1101	90-91	1.9886667188936293
1101	92-93	2.080735776652176
1101	94-95	2.10219473762524
1101	96-97	2.1581734700922333
1101	98-99	2.2379458607496083
1101	100-101	2.181633290321141
1102	1	0.5191062038881213
1102	2	0.5529182572155662
1102	3	0.5174347218913411
1102	4	0.5922548246468438
1102	5	0.5930055127209357
1102	6	0.6266637246726106
1102	7	0.6485410499279212
1102	8	0.6770578922167587
1102	9	0.6946316292647907
1102	10-11	0.6580556711268599
1102	12-13	0.6386329980302179
1102	14-15	1.037945543850043
1102	16-17	1.0421590917572559
1102	18-19	1.019646884739693
1102	20-21	1.0715332037800565
1102	22-23	1.0676430860929926
1102	24-25	1.2339323494605452
1102	26-27	1.2925008887641312
1102	28-29	1.3458140493161537
1102	30-31	1.3385689291269003
1102	32-33	1.4566771183576677
1102	34-35	1.3497958525086062
1102	36-37	1.3004562732644018
1102	38-39	1.2990844613380474
1102	40-41	1.3595096316178186
1102	42-43	1.3431073382206336
1102	44-45	1.2518813531862207
1102	46-47	1.2607538142821966
1102	48-49	1.2763228754457074
1102	50-51	1.3489702158562764
1102	52-53	1.2833995123692716
1102	54-55	1.2758523110821578
1102	56-57	1.280306981191913
1102	58-59	1.28043210258042
1102	60-61	1.3445226011534857
1102	62-63	1.372916708882002
1102	64-65	1.4142218245492302
1102	66-67	1.4342564884582458
1102	68-69	1.4064160517430437
1102	70-71	1.4177770989808138
1102	72-73	1.4403737471945668
1102	74-75	1.403825684494798
1102	76-77	1.4392614797447933
1102	78-79	1.5039047827421506
1102	80-81	1.5076241640951515
1102	82-83	1.5566377069854553
1102	84-85	1.48515519003276
1102	86-87	1.5369543516044573
1102	88-89	1.6406126408178459
1102	90-91	1.6698117824793144
1102	92-93	1.67468989603125
1102	94-95	1.68940288011283
1102	96-97	1.711704221608315
1102	98-99	1.8334932637302117
1102	100-101	1.806367970112916
1103	1	0.2697704867228161
1103	2	0.29120829762439726
1103	3	0.25766482625846976
1103	4	0.28506952173366784
1103	5	0.2845707265302977
1103	6	0.3335019400823853
1103	7	0.3888772936500331
1103	8	0.3847512491140179
1103	9	0.42879623775878173
1103	10-11	0.44372727331118256
1103	12-13	0.39143256886106315
1103	14-15	0.5872258356685052
1103	16-17	0.6958364333504505
1103	18-19	0.5872975227776251
1103	20-21	0.5793076615796053
1103	22-23	0.551690200879797
1103	24-25	0.5624736488804913
1103	26-27	0.6048752514452218
1103	28-29	0.6150461894792869
1103	30-31	0.5868288910837336
1103	32-33	0.7747648161006353
1103	34-35	0.7524852279858365
1103	36-37	0.6773525852423106
1103	38-39	0.7249905802102035
1103	40-41	0.7767438933868007
1103	42-43	0.6767223806078917
1103	44-45	0.5555656867089667
1103	46-47	0.5483598907323142
1103	48-49	0.4955452888217948
1103	50-51	0.3189215068980573
1103	52-53	0.3526713861922808
1103	54-55	0.35844200316038766
1103	56-57	0.23242208139981102
1103	58-59	0.257589427462527
1103	60-61	0.20165885946768114
1103	62-63	0.31052199909067113
1103	64-65	0.28951531488147
1103	66-67	0.3186468733161867
1103	68-69	0.37716572963770645
1103	70-71	0.40858925623281905
1103	72-73	0.1839013003219847
1103	74-75	0.2672648279609042
1103	76-77	0.3226166230930403
1103	78-79	0.32754500542587195
1103	80-81	0.32792909418950345
1103	82-83	0.3677823808251901
1103	84-85	0.35329801021641316
1103	86-87	0.24489232142584427
1103	88-89	0.15440501822730468
1103	90-91	0.32896400153743244
1103	92-93	0.3559440675406371
1103	94-95	0.4037880978286523
1103	96-97	0.3998766665697744
1103	98-99	0.35859205233405334
1103	100-101	0.43331081456830844
1104	1	0.2844101131344843
1104	2	0.33499023216421975
1104	3	0.33623749324646823
1104	4	0.3656263993806306
1104	5	0.32887817404889574
1104	6	0.35732403601173246
1104	7	-1.069006242871815
1104	8	0.10676174332328259
1104	9	0.3932578786684928
1104	10-11	0.41213233885358846
1104	12-13	0.48352294166210186
1104	14-15	0.6762738305931606
1104	16-17	0.7309298669182169
1104	18-19	0.7180180005435588
1104	20-21	0.7542051662491787
1104	22-23	0.7701152601292307
1104	24-25	0.8248917481424485
1104	26-27	0.8957281652543898
1104	28-29	0.973195405081853
1104	30-31	0.9390933043891039
1104	32-33	1.0641727813167847
1104	34-35	0.9193920187947171
1104	36-37	0.9029237189012278
1104	38-39	0.9550742008767656
1104	40-41	0.8615586040828092
1104	42-43	0.8885350783588848
1104	44-45	0.750106686286685
1104	46-47	0.6787460473910585
1104	48-49	0.7692930573897385
1104	50-51	0.5669519182986349
1104	52-53	0.8099264636271357
1104	54-55	0.6879918994461711
1104	56-57	0.7341515206721994
1104	58-59	0.7748400905519439
1104	60-61	0.7407877247681114
1104	62-63	0.7448124834180696
1104	64-65	0.8423436089837928
1104	66-67	0.7480548869235903
1104	68-69	0.5879880734657199
1104	70-71	0.6939884112771324
1104	72-73	0.6743002837939969
1104	74-75	0.6347999028981981
1104	76-77	0.7033165973870545
1104	78-79	0.5899264098087613
1104	80-81	0.6684777013017431
1104	82-83	0.6575682857270806
1104	84-85	0.7325973272035604
1104	86-87	0.7771038847839371
1104	88-89	0.7014415867283823
1104	90-91	0.7249713969733484
1104	92-93	0.8120027677536363
1104	94-95	0.7927393040587205
1104	96-97	0.7828763613953882
1104	98-99	0.7650363321519507
1104	100-101	0.8173767709494584
1105	1	0.13656904240854217
1105	2	0.11480950998465644
1105	3	0.12161336886195429
1105	4	0.13749438373887557
1105	5	0.03389372598132212
1105	6	0.16411795474842705
1105	7	-1.7744583463081085
1105	8	-0.394610637172498
1105	9	0.08200509461758543
1105	10-11	0.19234173479664918
1105	12-13	0.25314153949111073
1105	14-15	0.3676335720547286
1105	16-17	0.4880192390624032
1105	18-19	0.46323637675877904
1105	20-21	0.42608469940897464
1105	22-23	0.4065815152438077
1105	24-25	0.5461059582291341
1105	26-27	0.46182241500777366
1105	28-29	0.5617573898224606
1105	30-31	0.4294328626142203
1105	32-33	0.5396596208762929
1105	34-35	0.4956689412556088
1105	36-37	0.24766005129124835
1105	38-39	0.23074833911124415
1105	40-41	0.2370933920314897
1105	42-43	0.28714196320675
1105	44-45	0.19328151648223013
1105	46-47	0.16534753487097475
1105	48-49	0.24270970756322896
1105	50-51	0.2524336000262011
1105	52-53	0.3260887687543139
1105	54-55	0.06353457761343506
1105	56-57	0.13418316839983646
1105	58-59	0.14387521334244013
1105	60-61	0.050858147207478055
1105	62-63	0.0912413384309545
1105	64-65	0.0916685825177126
1105	66-67	0.091889570225689
1105	68-69	-0.0013829353569256853
1105	70-71	0.1989182219985608
1105	72-73	0.16710777291871182
1105	74-75	0.1202821711093307
1105	76-77	0.18387234992945878
1105	78-79	0.16772261481060724
1105	80-81	0.2004584298278651
1105	82-83	0.08474245103829858
1105	84-85	0.16590495554425289
1105	86-87	0.261564618607931
1105	88-89	0.3189970195432217
1105	90-91	0.28549071035170215
1105	92-93	0.3166913537555516
1105	94-95	0.22605937301414514
1105	96-97	0.2974035969041928
1105	98-99	0.2595754171358706
1105	100-101	0.3411972656471818
1106	1	-0.33330489912407657
1106	2	-0.3602098665585416
1106	3	-0.35999950447419593
1106	4	-0.3555571625794016
1106	5	-0.5742952173297269
1106	6	-0.5071846892674046
1106	7	-0.28771988967343276
1106	8	-0.31674171702515963
1106	9	0.08596895327385567
1106	10-11	-0.1796839418725611
1106	12-13	-0.4352806253199901
1106	14-15	-0.599174871205463
1106	16-17	-0.4875821752700489
1106	18-19	-0.6820096286996886
1106	20-21	-0.4769378420654107
1106	22-23	-0.43281489614297897
1106	24-25	-0.2691225849652099
1106	26-27	-0.4373638295740605
1106	28-29	-0.5925246547417871
1106	30-31	-0.7695392886967056
1106	32-33	-0.9761650532709041
1106	34-35	-0.5710704588191717
1106	36-37	-0.8070732552027096
1106	38-39	-0.7737620010996977
1106	40-41	-0.8672569139887543
1106	42-43	-0.5462275433142878
1106	44-45	-0.6741264653430186
1106	46-47	-0.8251238014104061
1106	48-49	-1.0259274575548076
1106	50-51	-0.7662473045772913
1106	52-53	-1.3519298544929548
1106	54-55	-1.3206481056927295
1106	56-57	-0.9254160847190178
1106	58-59	-0.7339910330220363
1106	60-61	-0.8265987153153631
1106	62-63	-0.6171966632985075
1106	64-65	-0.9721460238742416
1106	66-67	-0.907888335272883
1106	68-69	-0.8306467041219783
1106	70-71	-0.812918291862232
1106	72-73	-1.118412822087734
1106	74-75	-1.2671559793403198
1106	76-77	-0.9569770147965997
1106	78-79	-1.15054657417501
1106	80-81	-1.3048042799237436
1106	82-83	-1.1208527370492476
1106	84-85	-1.0471051755923888
1106	86-87	-1.0172329960903603
1106	88-89	-1.574214598327817
1106	90-91	-1.1109692500038904
1106	92-93	-1.2675178939808625
1106	94-95	-1.0691964663321833
1106	96-97	-1.0231408653744118
1106	98-99	-1.2990782001292516
1106	100-101	-1.0246777237094165
1107	1	0.14643772699083257
1107	2	0.12752607123461246
1107	3	0.22157208550785867
1107	4	0.23784475550375817
1107	5	0.2299328089878543
1107	6	0.2135516734952958
1107	7	0.37789160996506865
1107	8	0.344147188824401
1107	9	0.2978905162363574
1107	10-11	0.2996010159346483
1107	12-13	0.30071536799159304
1107	14-15	0.3870129067813508
1107	16-17	0.4700520393926695
1107	18-19	0.37750618842366634
1107	20-21	0.40381216629386074
1107	22-23	0.38368218671685383
1107	24-25	0.45517865005717795
1107	26-27	0.4472356247813991
1107	28-29	0.6463594834140096
1107	30-31	0.4173925667685907
1107	32-33	0.5794780881126727
1107	34-35	0.48390158532664174
1107	36-37	0.3282404760292792
1107	38-39	0.3157601582349514
1107	40-41	0.48012722219639414
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2216	84-85	-1.300800641321839
2216	86-87	-1.273015636271225
2216	88-89	-1.2831321242173566
2216	90-91	-1.2640670146255069
2216	92-93	-1.2710359137103673
2216	94-95	-1.2358707214908868
2216	96-97	-1.2868843127601934
2216	98-99	-1.3624439780939142
2216	100-101	-1.3105170773285728
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	337050.0
3	99866.0
4	86307.0
5	94154.0
6	131743.0
7	141806.0
8	136392.0
9	149392.0
10	151841.0
11	154306.0
12	151610.0
13	156590.0
14	170275.0
15	183429.0
16	141558.0
17	133415.0
18	135105.0
19	140062.0
20	151942.0
21	170231.0
22	196671.0
23	230219.0
24	272189.0
25	319526.0
26	370821.0
27	430987.0
28	501198.0
29	584568.0
30	685571.0
31	815979.0
32	985003.0
33	1216312.0
34	1526466.0
35	1974096.0
36	2734241.0
37	4162328.0
38	2728037.0
39	3580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.411004896049405	13.222516358193193	21.838487961933158	38.527990783824244
2	23.414563722213988	21.687519862340817	32.295914489317425	22.602001926127766
3	26.109718722200213	24.03620076354845	27.608021215188884	22.246059299062455
4	26.360253542345824	29.49900614512897	19.87347501031442	24.26726530221079
5	28.00800353270289	31.654552124622292	20.756955008813076	19.580489333861745
6	22.39470491686605	33.22186295816955	20.50878038013549	23.874651744828906
7	19.90308511532953	15.912353522161208	40.24751770346505	23.937043659044207
8	21.53214009159261	22.044468012422033	25.251344037315356	31.172047858670005
9	21.640099878736766	20.77477548848096	28.800447086466452	28.784677546315823
10-11	26.25679697410594	29.152687674739692	19.8760767393956	24.714438611758773
12-13	23.83801551679408	22.76135432035516	27.61340251668579	25.787227646164972
14-15	23.663883936262405	24.658281595920798	26.84190460798445	24.835929859832348
16-17	24.704777327735865	24.92755312979316	25.769879204436574	24.597790338034393
18-19	24.69473053025307	25.501455837222853	25.519015086996212	24.28479854552786
20-21	24.57642920945992	26.024112242282694	25.388595830149235	24.01086271810815
22-23	25.401454652041345	25.54731026410811	25.29053131727791	23.760703766572636
24-25	24.24463460732655	26.515994083123417	25.38568810795431	23.853683201595725
26-27	24.412020018871036	26.37195365567474	25.177925235426873	24.038101090027347
28-29	24.59889300352899	25.977909888105525	25.26042749686253	24.162769611502956
30-31	24.568549786630676	25.832894746455416	25.7186861072666	23.879869359647312
32-33	24.118086038869695	26.203564477329767	25.910854244594343	23.76749523920619
34-35	24.722456568741805	26.110522944182062	25.190381631305925	23.976638855770204
36-37	23.991781384058804	25.67483505474903	25.84312039230379	24.49026316888838
38-39	23.97859883155584	25.62053152280298	25.885954362383444	24.514915283257736
40-41	25.141741076831426	25.613919917731167	25.277307724929305	23.967031280508106
42-43	24.771960547691883	25.650986659344273	25.73826007566527	23.838792717298578
44-45	25.041377397870075	25.61544650040225	25.48050280028854	23.86267330143913
46-47	24.72570250718271	25.442373673879516	25.336133014685057	24.495790804252714
48-49	24.08697468025169	25.492563904061743	25.398426675914276	25.02203473977229
50-51	24.783888125439038	25.630710577439803	25.552151775613968	24.033249521507187
52-53	24.050053051306353	26.14493788916627	25.83058344408767	23.974425615439706
54-55	23.848984589869392	26.003537005044574	25.686447101098747	24.461031303987284
56-57	23.9817768642935	25.591058832664913	26.50167612395291	23.92548817908867
58-59	24.102794969332937	26.385386869031645	25.464081327625692	24.04773683400973
60-61	23.76694867685266	26.99630439445843	25.361261356821085	23.87548557186782
62-63	23.803331965032683	27.063231787557534	25.343009635916225	23.790426611493555
64-65	23.943260297300103	26.888043172166693	25.357706220477326	23.810990310055878
66-67	23.906900634969425	26.794860540017005	25.4182516946707	23.87998713034287
68-69	24.199276584382755	26.512179858202117	25.391750604444223	23.89679295297091
70-71	24.31882594023257	26.176572471218467	25.442654632463523	24.06194695608544
72-73	24.148785776874682	26.275511434077643	25.63283847511069	23.942864313936983
74-75	24.32513369722043	26.25263776339562	25.578470971259414	23.84375756812454
76-77	24.51830160684956	25.895585426916863	25.56247299761745	24.023639968616127
78-79	24.328868094018436	25.842228751587392	25.739438808363087	24.08946434603109
80-81	24.4854100962945	26.049157749939734	25.488775521896233	23.976656631869528
82-83	24.465852612131584	26.064616698569882	25.5122160716322	23.957314617666338
84-85	24.43801503312648	26.074621883176686	25.654822006511147	23.83254107718569
86-87	24.594019380546754	26.02374091098647	25.57769141611021	23.804548292356564
88-89	24.617753634128505	25.915932311098615	25.387306462901925	24.079007591870955
90-91	24.486992237360923	26.038002403857803	25.43467741309256	24.040327945688716
92-93	24.492385472386697	26.22055513652775	25.484827310010218	23.80223208107533
94-95	24.69891841974527	26.146345572560804	25.35321901603083	23.8015169916631
96-97	24.592068345168975	26.066912765325878	25.5621737487444	23.778845140760748
98-99	24.784013925786873	26.064159005975952	25.365958383903497	23.78586868433368
100-101	25.435799414475703	25.84303074726953	24.666164909192066	24.055004929062704
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10344.0
1	6180.0
2	1647.5
3	1257.5
4	1187.5
5	1165.5
6	1270.0
7	1571.5
8	2087.5
9	2704.5
10	3552.5
11	4648.0
12	5991.0
13	7427.0
14	8749.0
15	10076.0
16	11654.0
17	13439.5
18	15377.5
19	17903.5
20	21452.0
21	26790.5
22	34825.5
23	46025.5
24	60644.0
25	78729.5
26	100982.5
27	126443.0
28	153101.0
29	181562.0
30	211535.0
31	242817.0
32	275123.0
33	306698.5
34	339372.0
35	377997.0
36	420443.5
37	462321.0
38	500709.5
39	535211.0
40	567349.5
41	598404.0
42	629466.5
43	664227.0
44	689095.0
45	706201.0
46	725081.5
47	735065.5
48	746626.5
49	755373.5
50	762818.0
51	768855.5
52	765304.5
53	759267.0
54	741476.5
55	722079.0
56	696549.0
57	660271.5
58	630908.5
59	603928.0
60	574266.0
61	538672.0
62	498186.5
63	459984.0
64	421291.5
65	374048.5
66	318262.0
67	267743.5
68	222010.5
69	171576.0
70	123524.0
71	83926.5
72	54479.5
73	33890.0
74	20671.0
75	12511.5
76	7762.0
77	5054.5
78	3451.5
79	2424.5
80	1751.5
81	1310.0
82	980.5
83	710.0
84	535.0
85	404.0
86	302.5
87	223.5
88	174.5
89	129.5
90	84.0
91	62.0
92	47.5
93	36.5
94	25.5
95	23.0
96	20.0
97	17.0
98	18.0
99	17.5
100	37.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03854999629529789
2	0.03372026009733478
3	0.03247657006637613
4	0.033315950970662714
5	0.03256885801920345
6	0.029967216682357082
7	0.032885273857468554
8	0.06993668958542758
9	0.03727993827781715
10-11	0.03749088216999388
12-13	0.04094508840438788
14-15	0.0423623676799503
16-17	0.04208770115367851
18-19	0.039637675739334174
20-21	0.0597520547912697
22-23	0.04142630415841605
24-25	0.04436633465562926
26-27	0.0414306988228364
28-29	0.14036118692151384
30-31	0.15015689391447087
32-33	0.1453645123640807
34-35	0.11243968652682904
36-37	0.16870677243276228
38-39	0.31063245988792026
40-41	0.5948815519282777
42-43	0.6442885666740468
44-45	0.6358903629667606
46-47	0.6242818569004098
48-49	0.6152903734963766
50-51	0.6122756337040175
52-53	0.6213154584166745
54-55	0.632517458024143
56-57	0.6200212297448818
58-59	0.6298081474089982
60-61	0.6272922020283486
62-63	0.6332469723179209
64-65	0.6308035389002071
66-67	0.6308145255612579
68-69	0.6446994677973493
70-71	0.6449148063539465
72-73	0.6512409257870383
74-75	0.658221850218762
76-77	0.6659520649341553
78-79	0.6635987221370585
80-81	0.6644161297192434
82-83	0.6651038947010279
84-85	0.654174364287621
86-87	0.6566287843663856
88-89	0.665987222249518
90-91	0.6772683258165528
92-93	0.669228287259525
94-95	0.6684504316571234
96-97	0.6662333234570575
98-99	0.6688393594583242
100-101	0.6576857011594794
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.2754866E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.41235132603979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.37455373706884	42.101314016369344
2	12.072817443109285	13.138207684192077
3	4.157707532389846	6.786919288899548
4	1.912331525311135	4.1621781922836405
5	1.083383868912235	2.947473184810838
6	0.7166634666653716	2.3397206598440285
7	0.46966820739955456	1.788902605538712
8	0.3365963284154297	1.4651998141436344
9	0.24886079796351085	1.2186991053062246
>10	1.4666498190811017	14.923668955855005
>50	0.11112506167696226	4.160885184248793
>100	0.04863888219987173	4.468716656908028
>500	9.300725434421722E-4	0.3295324736545521
>1k	5.703423560543435E-5	0.039354467881854795
>5k	0.0	0.0
>10k+	1.6223027751403687E-5	0.12922771006369943
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.10532252749807448	1.3183993261045792E-5	0.0	0.0	0.0
2	0.10590701786598083	3.5157315362788776E-5	0.0	3.0762650942440176E-5	0.0
3	0.10596414850344538	3.5157315362788776E-5	0.0	3.0762650942440176E-5	0.0
4	0.10629374833497152	3.5157315362788776E-5	0.0	4.834130862383457E-5	0.0
5	0.10635966830127674	3.9551979783137375E-5	0.0	4.834130862383457E-5	0.0
6	0.10657500685787383	3.9551979783137375E-5	0.0	5.713063746453176E-5	0.0
7	0.10673760944142673	4.394664420348597E-5	0.0	5.713063746453176E-5	0.0
8	0.10717268121904124	5.713063746453176E-5	0.0	6.152530188488035E-5	0.0
9	0.10730891581607205	6.591996630522895E-5	0.0	6.152530188488035E-5	0.0
10-11	0.10762533165433714	7.470929514592615E-5	0.0	6.152530188488035E-5	2.1973322101742985E-6
12-13	0.10805820609974148	8.569595619679764E-5	0.0	7.470929514592615E-5	4.394664420348597E-6
14-15	0.10867785378301062	1.0107728166801773E-4	0.0	8.569595619679764E-5	4.394664420348597E-6
16-17	0.10942274940225971	1.1426127492906353E-4	0.0	9.887994945784344E-5	4.394664420348597E-6
18-19	0.11018302634698003	1.1865593934941212E-4	0.0	1.1426127492906352E-4	4.394664420348597E-6
20-21	0.1134087100315159	1.274452681901093E-4	0.0	1.296426004002836E-4	4.394664420348597E-6
22-23	0.11655968442090583	1.428265936613294E-4	0.0	1.3843192924098082E-4	4.394664420348597E-6
24-25	0.11738148666751103	1.47221258081678E-4	0.0	1.428265936613294E-4	4.394664420348597E-6
26-27	0.11829337953473336	1.538132547122009E-4	0.0	1.494185902918523E-4	6.591996630522896E-6
28-29	0.11941401896192225	1.647999157630724E-4	0.0	1.516159225020266E-4	1.538132547122009E-5
30-31	0.12036985847334808	1.9775989891568688E-4	2.1973322101742985E-6	1.538132547122009E-4	1.7578657681394388E-5
32-33	0.12162892982977794	2.0215456333603547E-4	4.394664420348597E-6	1.582079191325495E-4	2.1973322101742984E-5
34-35	0.12285723853526538	2.0215456333603547E-4	4.394664420348597E-6	1.626025835528981E-4	2.1973322101742984E-5
36-37	0.12421958450557344	2.0654922775638406E-4	4.394664420348597E-6	1.669972479732467E-4	2.6367986522091584E-5
38-39	0.12549843185189488	2.1094389217673267E-4	4.394664420348597E-6	1.7578657681394387E-4	3.0762650942440176E-5
40-41	0.12691131646303697	2.1094389217673267E-4	4.394664420348597E-6	1.9116790228516396E-4	3.0762650942440176E-5
42-43	0.1283769370472232	2.1753588880725556E-4	4.394664420348597E-6	2.0435189554620975E-4	3.0762650942440176E-5
44-45	0.1298930962722435	2.2852254985812707E-4	4.394664420348597E-6	2.1973322101742984E-4	3.2959983152614476E-5
46-47	0.13154768742650474	2.461012075395214E-4	6.591996630522896E-6	2.3511454648864993E-4	3.5157315362788776E-5
48-49	0.13325501455381017	2.5269320417004433E-4	8.789328840697194E-6	2.461012075395214E-4	3.7354647572963076E-5
50-51	0.13520624555644495	2.614825330107415E-4	1.3183993261045792E-5	2.5049587195987E-4	4.1749311993311675E-5
52-53	0.13746510306850412	2.768638584819616E-4	1.3183993261045792E-5	2.5489053638021864E-4	4.394664420348597E-5
54-55	0.1399612724592621	2.856531873226588E-4	1.3183993261045792E-5	2.5708786859039294E-4	4.614397641366027E-5
56-57	0.14257170312494918	3.0982384163457614E-4	1.3183993261045792E-5	2.7686385848196165E-4	4.834130862383457E-5
58-59	0.14655766375420537	3.252051671057962E-4	1.3183993261045792E-5	2.94442516163356E-4	5.273597304418317E-5
60-61	0.1501283285957386	3.274024993159705E-4	1.3183993261045792E-5	3.16415838265099E-4	5.273597304418317E-5
62-63	0.15442411306662934	3.4278382478719057E-4	1.3183993261045792E-5	3.274024993159705E-4	5.713063746453176E-5
64-65	0.15976143300514273	3.4937582141771344E-4	1.538132547122009E-5	3.4058649257701627E-4	5.713063746453176E-5
66-67	0.1647471797900282	3.5377048583806205E-4	1.7578657681394388E-5	3.4278382478719057E-4	5.713063746453176E-5
68-69	0.1697395185715442	3.5816515025841066E-4	1.7578657681394388E-5	3.647571468889336E-4	5.713063746453176E-5
70-71	0.17674681098979006	3.7354647572963076E-4	1.7578657681394388E-5	3.779411401499793E-4	5.713063746453176E-5
72-73	0.1865644913048488	3.801384723601536E-4	1.7578657681394388E-5	3.97717130041548E-4	5.713063746453176E-5
74-75	0.20111522520062303	3.9112513341102515E-4	1.7578657681394388E-5	4.109011233025938E-4	5.713063746453176E-5
76-77	0.21824342977893169	3.9332246562119946E-4	1.7578657681394388E-5	4.130984555127681E-4	5.713063746453176E-5
78-79	0.23982342941505347	4.0430912667207094E-4	1.7578657681394388E-5	4.174931199331167E-4	5.713063746453176E-5
80-81	0.2657255815085881	4.109011233025938E-4	1.7578657681394388E-5	4.2628244877381395E-4	6.152530188488035E-5
82-83	0.3055632144790481	4.2628244877381395E-4	1.7578657681394388E-5	4.350717776145111E-4	6.591996630522895E-5
84-85	0.3655745544711184	4.614397641366027E-4	1.7578657681394388E-5	4.438611064552083E-4	6.591996630522895E-5
86-87	0.4336501036745284	4.658344285569513E-4	1.7578657681394388E-5	4.922024150790429E-4	6.591996630522895E-5
88-89	0.5030440522040429	4.7242642518747417E-4	1.7578657681394388E-5	5.097810727604373E-4	7.031463072557755E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGAA	36705	0.0	34.846287	2
GATCGGA	37450	0.0	34.508064	1
TCGGAAG	35765	0.0	33.937748	3
CGCCGTA	8420	0.0	30.368801	46-47
CGGAAGA	36595	0.0	29.690994	4
AAGAGCG	26445	0.0	28.280506	7
GATCTCG	21645	0.0	27.593473	34-35
CCGTATC	10740	0.0	27.019703	48-49
TGATACG	2645	0.0	25.752617	3
ATACGGC	2430	0.0	25.111397	5
GCCGGAT	11060	0.0	24.861881	46-47
GGCGCCG	14105	0.0	24.80804	44-45
AGAGCGT	20450	0.0	24.242231	8
ATCGGGA	6325	0.0	23.706846	2
TCGGGGG	20555	0.0	23.674221	38-39
GATCGGG	6575	0.0	23.445236	1
TAGATCT	19580	0.0	23.43202	32-33
GAGCGTC	20500	0.0	23.113348	9
AGAGCGG	10855	0.0	23.05316	8
TACGGCG	2820	0.0	22.645536	6
>>END_MODULE