FastQCFastQC Report
Wed 25 May 2016
SRR1033291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22754866
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2266510.9960550855364299TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG506300.050.304483
ATCGGAA511200.050.0740242
GATCGGA515200.049.792171
CGGAAGA525500.048.5120164
AGAGCAC548350.046.604028
GAGCACA563500.045.5785759
AAGAGCA641000.039.9490747
CGTATGC356600.038.44154444-45
CTCGTAT323500.038.22450342-43
TATGCCG359900.038.01973746-47
GAAGAGC688000.037.2745976
CACGATC340100.036.16968536-37
TCGTATG349500.036.049542-43
ATCTCGT343850.035.76247440-41
ATCACGA390500.035.57136534-35
TCTCGTA333450.034.2263840-41
CCGTCTT401850.033.98321550-51
TGCCGTC404300.033.74521648-49
ACGATCT338900.033.6980536-37
TCACGAT378150.033.19913534-35
Produced by FastQC (version 0.11.5)