##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033291_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22754866 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46603974727867 33.0 33.0 33.0 33.0 33.0 2 32.569801773387724 33.0 33.0 33.0 33.0 33.0 3 32.5231233618339 33.0 33.0 33.0 33.0 33.0 4 36.28291829976059 37.0 37.0 37.0 37.0 37.0 5 36.39266603459673 37.0 37.0 37.0 37.0 37.0 6 36.254416308142616 37.0 37.0 37.0 33.0 37.0 7 36.21687027293415 37.0 37.0 37.0 33.0 37.0 8 36.23106802738368 37.0 37.0 37.0 33.0 37.0 9 36.24255067026103 37.0 37.0 37.0 33.0 37.0 10-11 36.26250804992655 37.0 37.0 37.0 33.0 37.0 12-13 36.20173032440621 37.0 37.0 37.0 33.0 37.0 14-15 37.95960351952853 40.0 37.0 40.0 33.0 40.0 16-17 37.91058472943765 40.0 37.0 40.0 33.0 40.0 18-19 37.877759618536096 40.0 37.0 40.0 33.0 40.0 20-21 37.89828061391353 40.0 37.0 40.0 33.0 40.0 22-23 37.82133054529963 40.0 37.0 40.0 33.0 40.0 24-25 37.12878717897087 38.5 37.0 40.0 33.0 40.0 26-27 36.04599394257035 37.0 37.0 40.0 27.0 40.0 28-29 35.84715959215053 37.0 37.0 40.0 27.0 40.0 30-31 35.67403528106911 37.0 37.0 40.0 27.0 40.0 32-33 35.523274845916475 37.0 37.0 40.0 27.0 40.0 34-35 35.61563511294683 37.0 37.0 40.0 27.0 40.0 36-37 35.632573116449024 37.0 37.0 40.0 27.0 40.0 38-39 35.55762609193128 37.0 37.0 40.0 27.0 40.0 40-41 35.4206172429229 37.0 37.0 40.0 27.0 40.0 42-43 35.184894804478304 37.0 33.0 40.0 27.0 40.0 44-45 34.98543915837606 37.0 33.0 40.0 27.0 40.0 46-47 34.9000287674733 37.0 33.0 40.0 27.0 40.0 48-49 34.74174934715063 37.0 33.0 40.0 27.0 40.0 50-51 34.64506932275496 37.0 33.0 40.0 27.0 40.0 52-53 34.51875871297155 37.0 33.0 40.0 24.5 40.0 54-55 34.35569653541357 37.0 33.0 40.0 24.5 40.0 56-57 34.18884384992643 37.0 33.0 40.0 24.5 40.0 58-59 33.97234844186734 37.0 33.0 40.0 22.0 40.0 60-61 33.72158730796305 37.0 33.0 40.0 22.0 40.0 62-63 33.49794723027593 37.0 33.0 37.0 22.0 40.0 64-65 33.19435559409578 37.0 33.0 37.0 22.0 40.0 66-67 32.92909037565855 37.0 33.0 37.0 22.0 40.0 68-69 32.63761210459336 37.0 33.0 37.0 22.0 40.0 70-71 32.33776226148728 37.0 33.0 37.0 22.0 40.0 72-73 32.01832186135484 37.0 33.0 37.0 22.0 38.5 74-75 31.63590286578704 37.0 33.0 37.0 15.0 37.0 76-77 31.408963559706308 37.0 33.0 37.0 15.0 37.0 78-79 31.162566371518075 35.0 33.0 37.0 15.0 37.0 80-81 30.924000760980093 33.0 33.0 37.0 15.0 37.0 82-83 30.718285882237232 33.0 33.0 37.0 15.0 37.0 84-85 30.540061101656235 33.0 27.0 37.0 15.0 37.0 86-87 30.34065379686261 33.0 27.0 37.0 15.0 37.0 88-89 30.119236606359273 33.0 27.0 37.0 6.0 37.0 90-91 29.881124327429568 33.0 27.0 37.0 6.0 37.0 92-93 29.621692037210853 33.0 27.0 37.0 2.0 37.0 94-95 29.379713200684197 33.0 27.0 37.0 2.0 37.0 96-97 29.217705566800525 33.0 27.0 37.0 2.0 37.0 98-99 28.911099762134395 33.0 27.0 37.0 2.0 37.0 100-101 26.475117739651818 30.0 21.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20283701011918254 1101 2 0.15017811349363086 1101 3 0.24524700085470386 1101 4 0.3573492937832725 1101 5 0.2343235529166634 1101 6 0.2865506818548482 1101 7 0.29407939185677634 1101 8 0.307946161621345 1101 9 0.30475590217047 1101 10-11 0.29068275954168854 1101 12-13 0.31080814712110794 1101 14-15 0.7272754296127673 1101 16-17 0.7591481668218947 1101 18-19 0.7613116679259306 1101 20-21 0.7123521773040835 1101 22-23 0.7394316800271667 1101 24-25 0.861344417217687 1101 26-27 1.0227117821762164 1101 28-29 1.100197444221422 1101 30-31 1.1492773360568762 1101 32-33 1.2007975874169574 1101 34-35 1.093177442708189 1101 36-37 1.0862184107982884 1101 38-39 1.0651082511166763 1101 40-41 1.0553066748045978 1101 42-43 1.1178360946031418 1101 44-45 1.2411561123207946 1101 46-47 1.2206497989042333 1101 48-49 1.158408983039216 1101 50-51 1.172834297967114 1101 52-53 1.082818295239278 1101 54-55 1.1290717364664005 1101 56-57 1.1194887910938291 1101 58-59 1.1305997375572474 1101 60-61 1.1766664027362381 1101 62-63 1.106695773887047 1101 64-65 1.1541543022411531 1101 66-67 1.2043811303655332 1101 68-69 1.182222913769266 1101 70-71 1.2144735258153858 1101 72-73 1.218952983566858 1101 74-75 1.22177423264095 1101 76-77 1.2365211337424356 1101 78-79 1.247853846817165 1101 80-81 1.3247126309071646 1101 82-83 1.3271158911659633 1101 84-85 1.2839375495040706 1101 86-87 1.3359911791558723 1101 88-89 1.413334017014936 1101 90-91 1.4577194599947028 1101 92-93 1.517908746585256 1101 94-95 1.5605224496265677 1101 96-97 1.6376379967214874 1101 98-99 1.7264136732805895 1101 100-101 1.9111299500875703 1102 1 0.15181753076063842 1102 2 0.14008037523542072 1102 3 0.21445086585082862 1102 4 0.29986067664606963 1102 5 0.19386382751595477 1102 6 0.21702863535779215 1102 7 0.22825652587111733 1102 8 0.21791777229655906 1102 9 0.21999871567926732 1102 10-11 0.24932443787563585 1102 12-13 0.2737658514808601 1102 14-15 0.6170148043210872 1102 16-17 0.6682288548407556 1102 18-19 0.6320989469236551 1102 20-21 0.6015649061936585 1102 22-23 0.5924646204176938 1102 24-25 0.729025324759796 1102 26-27 0.8803062819091494 1102 28-29 0.8894280920989175 1102 30-31 0.906168524217172 1102 32-33 1.0075103383881512 1102 34-35 0.9340393311678952 1102 36-37 0.9303430713846126 1102 38-39 0.8862576268111511 1102 40-41 0.9026008897023416 1102 42-43 0.96548688584096 1102 44-45 1.1321109655604857 1102 46-47 0.9674002125352033 1102 48-49 0.9403651580869621 1102 50-51 0.9856924436302847 1102 52-53 0.9634752939894824 1102 54-55 0.9523077125409856 1102 56-57 0.9517045072499926 1102 58-59 0.9146307197703152 1102 60-61 0.933263659169917 1102 62-63 0.95262232966639 1102 64-65 0.973025215159403 1102 66-67 0.9831656688918642 1102 68-69 0.9995694409336622 1102 70-71 1.0730466996289536 1102 72-73 1.0633262970109527 1102 74-75 1.0155334476349047 1102 76-77 1.0544395506864959 1102 78-79 1.0467805225646316 1102 80-81 1.0358870974114254 1102 82-83 1.1258357200586069 1102 84-85 1.0926264394732392 1102 86-87 1.157325722474301 1102 88-89 1.1857819291542846 1102 90-91 1.2089391449299285 1102 92-93 1.26080157135695 1102 94-95 1.296304267318611 1102 96-97 1.3730011151917978 1102 98-99 1.4311213757444676 1102 100-101 1.5804729851131327 1103 1 0.1307921079096559 1103 2 0.09296237144240393 1103 3 0.10863047419857708 1103 4 0.24026347418367067 1103 5 0.14864810958057006 1103 6 0.16496527470646072 1103 7 0.144336913284981 1103 8 0.189085774520791 1103 9 0.21482780111970357 1103 10-11 0.22798020582933987 1103 12-13 0.21831483306018384 1103 14-15 0.5010719840722615 1103 16-17 0.4992180220648166 1103 18-19 0.4729690852469375 1103 20-21 0.4533640672235819 1103 22-23 0.483560898647859 1103 24-25 0.5942363183589165 1103 26-27 0.8030317134581324 1103 28-29 0.8541458247731484 1103 30-31 0.8412283065987936 1103 32-33 0.8891732606520648 1103 34-35 0.7756220205889548 1103 36-37 0.7191411011600124 1103 38-39 0.7385620106894137 1103 40-41 0.7435199878256924 1103 42-43 0.8104739729476975 1103 44-45 0.9382560144780356 1103 46-47 0.8168787388454888 1103 48-49 0.7863824862522222 1103 50-51 0.7740540163654259 1103 52-53 0.8154294597477261 1103 54-55 0.8255025617084044 1103 56-57 0.7600871245201475 1103 58-59 0.7073247689266395 1103 60-61 0.7376675674732951 1103 62-63 0.6876344088641417 1103 64-65 0.6801560812440641 1103 66-67 0.6439747138201142 1103 68-69 0.5957959053426549 1103 70-71 0.6072516666656398 1103 72-73 0.5637559909676426 1103 74-75 0.5561957083673228 1103 76-77 0.5002720096313347 1103 78-79 0.5267022924573155 1103 80-81 0.4951344216900537 1103 82-83 0.4863215275479007 1103 84-85 0.5161020011839241 1103 86-87 0.6424329648446623 1103 88-89 0.5397610151799803 1103 90-91 0.5667478863928679 1103 92-93 0.543273677708207 1103 94-95 0.5768935600865355 1103 96-97 0.569384292619862 1103 98-99 0.6504674650017357 1103 100-101 0.8716818664861279 1104 1 0.10735609516008537 1104 2 0.05031594910745696 1104 3 -0.009031138947889872 1104 4 0.2289876551657315 1104 5 0.12501725012724307 1104 6 0.12256975318614138 1104 7 0.15225272447817417 1104 8 0.010472218563137403 1104 9 0.10039941909167283 1104 10-11 0.1228258571055747 1104 12-13 0.11923091569589417 1104 14-15 0.32260277714473773 1104 16-17 0.27349374593149633 1104 18-19 0.3373351366839472 1104 20-21 0.3824467789210928 1104 22-23 0.3924823845655965 1104 24-25 0.389506311215726 1104 26-27 0.5068301753401698 1104 28-29 0.5538576727074869 1104 30-31 0.6218401567447813 1104 32-33 0.5817363979642209 1104 34-35 0.6016523116777392 1104 36-37 0.5016134010641338 1104 38-39 0.5947984209492674 1104 40-41 0.5701967445357496 1104 42-43 0.6269081403410937 1104 44-45 0.6169744782284425 1104 46-47 0.6980760643392543 1104 48-49 0.6787075076530655 1104 50-51 0.7334465297704469 1104 52-53 0.5477871943633517 1104 54-55 0.6821405128578846 1104 56-57 0.6528629983705798 1104 58-59 0.6223476362147409 1104 60-61 0.5217469912965313 1104 62-63 0.5323192036488607 1104 64-65 0.6197424064572914 1104 66-67 0.5765393017410716 1104 68-69 0.6977050653202497 1104 70-71 0.7964076228747103 1104 72-73 0.7555473578983225 1104 74-75 0.7734762853704886 1104 76-77 0.722226322288499 1104 78-79 0.7247068804432288 1104 80-81 0.7017869916220221 1104 82-83 0.7451281765452897 1104 84-85 0.7136737132147957 1104 86-87 0.7754071513995449 1104 88-89 0.8166329852603944 1104 90-91 0.856294669690147 1104 92-93 0.8545675753557376 1104 94-95 0.8528949910184167 1104 96-97 0.8966934245035887 1104 98-99 0.9612993144883824 1104 100-101 1.0034295669203495 1105 1 0.11209349271170055 1105 2 0.0345363160001142 1105 3 -0.0026080998821029766 1105 4 0.20599860254854008 1105 5 0.14337592277399835 1105 6 0.026729572769113474 1105 7 -0.026968884506082702 1105 8 0.0362159603089367 1105 9 0.08380781873973575 1105 10-11 0.11990441234387816 1105 12-13 0.07617866593777478 1105 14-15 0.0981711603046378 1105 16-17 0.10729429624827702 1105 18-19 0.1172405908772376 1105 20-21 0.32479616886715235 1105 22-23 0.35248509725354893 1105 24-25 0.34367328858437673 1105 26-27 0.40252614795484476 1105 28-29 0.4200889375571606 1105 30-31 0.39016693811653624 1105 32-33 0.5207733457483101 1105 34-35 0.4797678431020458 1105 36-37 0.34802665382339626 1105 38-39 0.41168815193902475 1105 40-41 0.23766849644292165 1105 42-43 0.4306605606826608 1105 44-45 0.5665043556890126 1105 46-47 0.4217208315484413 1105 48-49 0.4354883625802799 1105 50-51 0.41681436893146895 1105 52-53 0.4536106976948986 1105 54-55 0.4055913939728839 1105 56-57 0.46091911480140624 1105 58-59 0.3791418987830184 1105 60-61 0.39803365654776 1105 62-63 0.3843979780797966 1105 64-65 0.3600552228226448 1105 66-67 0.42332565833182656 1105 68-69 0.47143094186336754 1105 70-71 0.428015915533841 1105 72-73 0.39141320605957475 1105 74-75 0.3445147052910009 1105 76-77 0.278938039309665 1105 78-79 0.4689380257397957 1105 80-81 0.46103824784295355 1105 82-83 0.4712313163150519 1105 84-85 0.5464748570350366 1105 86-87 0.5388326511175698 1105 88-89 0.5884943741408648 1105 90-91 0.6081642091700026 1105 92-93 0.5931514498119341 1105 94-95 0.45374414579799804 1105 96-97 0.49690497315472015 1105 98-99 0.6123096833029145 1105 100-101 0.7164794897265772 1106 1 -6.103231165255352E-4 1106 2 -0.03800173448780697 1106 3 -0.08661908149436925 1106 4 0.09558500100843759 1106 5 -0.18221794442074923 1106 6 -0.10624550431745661 1106 7 -0.01718841098804802 1106 8 -0.07247588601562427 1106 9 -0.050341808716382275 1106 10-11 -0.20019648842301052 1106 12-13 -0.005064714749195787 1106 14-15 -0.2913497999303942 1106 16-17 -0.1683645541205081 1106 18-19 0.004971615870886126 1106 20-21 0.08153774303109174 1106 22-23 0.0850288340565939 1106 24-25 -0.039703618500112725 1106 26-27 0.008558607314682831 1106 28-29 -0.2641070725112016 1106 30-31 -0.30861358793831073 1106 32-33 -0.07604990515319088 1106 34-35 -0.17301473043244187 1106 36-37 0.032989762709192405 1106 38-39 -0.1377356751583747 1106 40-41 -0.5989494991369355 1106 42-43 -0.02225090623796433 1106 44-45 0.21114306276832906 1106 46-47 0.10585954832274069 1106 48-49 -0.15249997210077026 1106 50-51 -0.35571981601943037 1106 52-53 -0.09388914097760193 1106 54-55 -0.15964430741046698 1106 56-57 -0.08403506033494779 1106 58-59 0.11997107458039835 1106 60-61 0.08706836000751395 1106 62-63 -0.10063847829322725 1106 64-65 -0.8885882208471969 1106 66-67 -0.46741790659805105 1106 68-69 -0.18407536090672494 1106 70-71 -0.5934573121878515 1106 72-73 -0.6365626577380255 1106 74-75 -0.36733852091014185 1106 76-77 -0.37880568514665924 1106 78-79 -0.24194784958217497 1106 80-81 -0.26403508527425146 1106 82-83 -0.34586131911937557 1106 84-85 -0.5077317238161356 1106 86-87 -0.29934866439203134 1106 88-89 -0.33813095436751084 1106 90-91 -0.9996669626431647 1106 92-93 -1.120512345072072 1106 94-95 -1.6192371950871447 1106 96-97 -1.2629854722570748 1106 98-99 -1.1478834379686873 1106 100-101 -0.9818424370632357 1107 1 0.09284097292421478 1107 2 0.033965152923912 1107 3 0.06352677011622632 1107 4 0.21697175487307163 1107 5 0.13734628341138944 1107 6 0.11994139011388683 1107 7 0.15140328973625117 1107 8 0.11702651060848268 1107 9 0.12261891539451142 1107 10-11 0.10218279393415486 1107 12-13 0.09785770483102851 1107 14-15 0.12472509059649894 1107 16-17 0.07297150172303901 1107 18-19 0.23239868731179314 1107 20-21 0.18741023483586616 1107 22-23 0.24787480577753485 1107 24-25 0.30774196658259 1107 26-27 0.30396096687550767 1107 28-29 0.35033397338207095 1107 30-31 0.34587594394017174 1107 32-33 0.4954084767580369 1107 34-35 0.3804281825170577 1107 36-37 0.26284343159116474 1107 38-39 0.25847190465929515 1107 40-41 0.2531675946091738 1107 42-43 0.35179676896505896 1107 44-45 0.3255804290102091 1107 46-47 0.3593358994410565 1107 48-49 0.11703994295164932 1107 50-51 0.2033832186880744 1107 52-53 0.2362875894039007 1107 54-55 0.2868958058527511 1107 56-57 0.3182060791989656 1107 58-59 0.310340612803472 1107 60-61 0.33379682146995293 1107 62-63 0.37882824990175124 1107 64-65 0.3368463943804372 1107 66-67 0.39836413527662984 1107 68-69 0.4202587420208488 1107 70-71 0.22141283320697625 1107 72-73 0.32339338493775216 1107 74-75 0.33021778545044 1107 76-77 0.38764589537450433 1107 78-79 0.46332500780393104 1107 80-81 0.42851443323650074 1107 82-83 0.4406356454646385 1107 84-85 0.38018066835722664 1107 86-87 0.3950673449408697 1107 88-89 0.3314430586659043 1107 90-91 0.4108199362590206 1107 92-93 0.47337673342285314 1107 94-95 0.45978121268828076 1107 96-97 0.517932444578932 1107 98-99 0.4844876215111995 1107 100-101 0.7011437077116831 1108 1 0.09934527404130478 1108 2 0.03121745788535435 1108 3 0.14873404614799313 1108 4 0.2575481073189323 1108 5 0.1637314193246553 1108 6 0.12453404091889553 1108 7 0.1412311904693624 1108 8 0.13471269061991364 1108 9 0.09847803709827474 1108 10-11 0.09968010543438055 1108 12-13 0.08858966986726102 1108 14-15 0.19139376055182566 1108 16-17 0.15809463630839815 1108 18-19 0.21350559549312464 1108 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-0.7888484803826401 2215 28-29 -0.819231973546529 2215 30-31 -0.8329473502144396 2215 32-33 -0.9311525480816982 2215 34-35 -0.9183328252125804 2215 36-37 -0.8481939403607086 2215 38-39 -0.9570407609000711 2215 40-41 -0.8706314543617708 2215 42-43 -0.9278374441129102 2215 44-45 -1.0215495895365265 2215 46-47 -0.9684064827475822 2215 48-49 -0.9025544528299747 2215 50-51 -0.8636124363121169 2215 52-53 -0.8580412505000652 2215 54-55 -0.8695289104071549 2215 56-57 -0.9040168828810451 2215 58-59 -0.8854389754767737 2215 60-61 -0.8603090081223215 2215 62-63 -0.9051153771451723 2215 64-65 -0.8902705992342277 2215 66-67 -0.8779730645270618 2215 68-69 -0.87051926186275 2215 70-71 -0.8576988385754056 2215 72-73 -0.8748215905712406 2215 74-75 -0.8577018157542362 2215 76-77 -0.8651472835559701 2215 78-79 -0.8816540195619424 2215 80-81 -0.9205417764282053 2215 82-83 -0.9261335875106163 2215 84-85 -0.9212243092456518 2215 86-87 -0.9495896086790232 2215 88-89 -0.9095482171512899 2215 90-91 -0.9655956153649008 2215 92-93 -1.0226993163122557 2215 94-95 -1.0052187380319069 2215 96-97 -1.012574025596308 2215 98-99 -1.0693128680685504 2215 100-101 -1.111627319700549 2216 1 -0.18606311730571434 2216 2 -0.1593495867310537 2216 3 -0.09605137870755698 2216 4 -0.03277559604056535 2216 5 -0.16460512749286949 2216 6 -0.20852566670551909 2216 7 -0.2455301417653004 2216 8 -0.24952749821805753 2216 9 -0.2568900385730828 2216 10-11 -0.3004808736463005 2216 12-13 -0.35088944314855297 2216 14-15 -0.7014186969058684 2216 16-17 -0.7632160940217219 2216 18-19 -0.7312888034536158 2216 20-21 -0.8095721880637328 2216 22-23 -0.8914630513239956 2216 24-25 -0.8899618343059572 2216 26-27 -1.0918954207384957 2216 28-29 -1.0722228264574056 2216 30-31 -1.1893638549664445 2216 32-33 -1.2599030866529333 2216 34-35 -1.2482104525066617 2216 36-37 -1.3625257405495574 2216 38-39 -1.305891453807881 2216 40-41 -1.278631313419396 2216 42-43 -1.1697850619825658 2216 44-45 -1.3320243467101704 2216 46-47 -1.4247778436766154 2216 48-49 -1.282053384119429 2216 50-51 -1.2512451468535772 2216 52-53 -1.29928796509639 2216 54-55 -1.2820568089230449 2216 56-57 -1.4718099241496034 2216 58-59 -1.469339543399549 2216 60-61 -1.4092179262138487 2216 62-63 -1.5001717788073918 2216 64-65 -1.4715119029972783 2216 66-67 -1.406098627447676 2216 68-69 -1.409004072894266 2216 70-71 -1.387861325939717 2216 72-73 -1.5538715094708664 2216 74-75 -1.4639954603404028 2216 76-77 -1.412999501686997 2216 78-79 -1.3750341525861884 2216 80-81 -1.369944760462186 2216 82-83 -1.4008054647189319 2216 84-85 -1.436087691254329 2216 86-87 -1.3894325931056102 2216 88-89 -1.507222393792862 2216 90-91 -1.5268773412684808 2216 92-93 -1.4904335879602968 2216 94-95 -1.5356656614571413 2216 96-97 -1.667044592190841 2216 98-99 -1.8203140035087912 2216 100-101 -1.699647982476442 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7706.0 3 7257.0 4 4528.0 5 8210.0 6 13291.0 7 23840.0 8 41563.0 9 61784.0 10 68903.0 11 70162.0 12 73983.0 13 79147.0 14 83173.0 15 87595.0 16 92429.0 17 98794.0 18 105934.0 19 116402.0 20 130115.0 21 147414.0 22 174215.0 23 209548.0 24 254592.0 25 308577.0 26 371892.0 27 455888.0 28 496968.0 29 554118.0 30 660926.0 31 790732.0 32 960971.0 33 1186832.0 34 1501180.0 35 1959358.0 36 2788948.0 37 4891501.0 38 3859754.0 39 6636.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.641413325487328 13.348175155303476 20.180525825680203 39.82988569352899 2 22.67265899352447 22.194409326745255 31.973242312827573 23.159689366902704 3 25.664965919128797 24.270809986508283 27.346899761367983 22.71732433299494 4 25.580102907328474 30.127842680691874 19.676045212706818 24.61600919927284 5 28.10639228700525 31.642694116351922 20.69613745995633 19.554776136686495 6 22.37333951613701 33.49334927383191 20.284819202440303 23.84849200759078 7 19.359468843256156 16.142622687500012 40.317791382194535 24.180117087049297 8 21.538276692114998 22.051533944431927 25.11345924867235 31.296730114780726 9 21.82579958539553 20.651883815294592 28.824389195392126 28.697927403917756 10-11 26.153734494612795 29.295561808142086 19.781423349015974 24.76928034822915 12-13 22.97426078848935 23.28346734283595 27.68164981414515 26.060622054529553 14-15 23.333630413562044 25.250782296620017 26.537425341013826 24.878161948804113 16-17 24.589423360125892 24.912154898481756 25.362514307953838 25.135907433438515 18-19 23.93086656565823 25.34456861544622 25.84252365719323 24.88204116170232 20-21 24.469383776585087 25.374544097472963 25.96604180978625 24.190030316155703 22-23 24.145498041451624 26.746289353620806 25.24151430839201 23.866698296535557 24-25 23.944212635662193 25.601170316713795 25.88026886205351 24.574348185570503 26-27 23.94591291809211 25.514229457898445 25.209851244462172 25.330006379547264 28-29 24.56708745244608 25.924791051950386 25.294988002443418 24.213133493160115 30-31 23.7633587660456 25.643649412011587 26.09951553512247 24.493476286820346 32-33 23.825729498525803 26.280407441841458 25.434295458396733 24.45956760123601 34-35 24.09256672180876 26.04420952781401 25.83677128105517 24.026452469322063 36-37 23.890221800718248 25.91845990077194 25.412085986283483 24.77923231222633 38-39 24.52866245985053 25.533307266876644 25.29292133096787 24.645108942304958 40-41 24.048808526002922 26.083955418034595 25.93740309682337 23.929832959139116 42-43 23.76375001448374 25.54320394094874 26.265983698225693 24.42706234634183 44-45 24.52426180572002 25.4184197547914 25.53026588550718 24.5270525539814 46-47 24.10499066943781 25.871536633203 25.958073230767987 24.065399466591202 48-49 24.42936449471179 25.4089686608149 26.1236919243878 24.03797492008551 50-51 23.949484014962945 25.48913301909587 25.431686840252294 25.129696125688888 52-53 24.567615495044432 25.517255442613802 25.949187834337184 23.96594122800458 54-55 23.75090918108035 25.429961541318942 26.25418757253041 24.564941705070304 56-57 23.916642814555296 25.37260821332678 26.211462030492992 24.499286941624927 58-59 24.623493717783266 25.37670184478344 25.947889563489408 24.051914873943886 60-61 23.74700866179568 25.64914686819074 26.08554363712799 24.51830083288559 62-63 24.31517504871266 25.74327179074577 26.16216900596119 23.779384154580388 64-65 23.937501983092318 26.802794619840874 25.440765065371075 23.818938331695737 66-67 23.81753643374564 26.826281903835426 25.502198079303124 23.853983583115802 68-69 23.958719422913763 26.84490209698444 25.395146251355644 23.80123222874615 70-71 23.91974973616632 26.82357479055249 25.34758719299863 23.909088280282557 72-73 23.762759139078206 26.93138250078027 25.529499932014545 23.77635842812698 74-75 24.000191871048592 26.586232149202726 25.559939575122087 23.853636404626595 76-77 24.230072372212607 26.11566906172948 25.584749653107163 24.069508912950752 78-79 24.100954934210556 25.949363973402434 25.76854990049161 24.181131191895393 80-81 24.21984531084886 26.09703258137609 25.589880722063192 24.09324138571186 82-83 24.222697331959868 26.032226105745735 25.641395948315804 24.103680613978593 84-85 24.16900469284122 26.12348206295853 25.786056508089622 23.921456736110624 86-87 24.27364372439209 26.124075849708895 25.67653310358961 23.9257473223094 88-89 24.371713784639528 25.90065296157123 25.524010544765797 24.203622709023442 90-91 24.198413890004296 26.053447307702314 25.51147775212535 24.23666105016804 92-93 24.29201341769019 26.195851341339356 25.585697596221713 23.926437644748745 94-95 24.422609050147162 26.176026301808193 25.447049974644294 23.95431467340035 96-97 24.304703128434603 26.13759964868313 25.607413816097512 23.95028340678475 98-99 24.4816327883196 26.091046738323044 25.448277944389798 23.979042528967558 100-101 24.72422954788503 26.051983865507662 25.022663377886566 24.20112320872074 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1823.0 1 1104.0 2 346.0 3 282.5 4 255.5 5 243.5 6 236.5 7 251.5 8 266.0 9 290.0 10 307.5 11 329.0 12 392.5 13 492.5 14 635.0 15 856.5 16 1326.0 17 2144.0 18 3433.5 19 5560.0 20 9101.0 21 14606.5 22 22731.0 23 34105.5 24 49009.5 25 67974.0 26 91855.0 27 118880.5 28 146863.0 29 175779.5 30 206328.5 31 239529.5 32 273337.0 33 305546.0 34 338821.5 35 377768.0 36 421576.0 37 465399.5 38 505744.5 39 543987.5 40 579339.5 41 614040.5 42 650838.5 43 693145.0 44 722850.5 45 741515.0 46 761632.0 47 769195.5 48 777312.5 49 779127.0 50 778398.0 51 778173.0 52 767872.0 53 758278.5 54 738166.0 55 717462.5 56 693536.0 57 658436.5 58 630990.5 59 606119.5 60 577276.5 61 542262.0 62 500567.0 63 461759.5 64 421946.0 65 371501.0 66 312140.0 67 260148.5 68 213455.0 69 161650.5 70 112990.0 71 73299.5 72 44860.0 73 25713.5 74 14091.5 75 7211.0 76 3732.5 77 2036.5 78 1175.5 79 687.0 80 408.0 81 278.5 82 199.5 83 139.5 84 88.5 85 57.0 86 44.5 87 29.5 88 18.0 89 17.0 90 15.5 91 10.0 92 5.5 93 3.5 94 4.0 95 4.0 96 2.5 97 1.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03069673097613495 2 0.0019028896940109425 3 7.295142937778672E-4 4 1.0547194608836632E-4 5 9.228795282732054E-5 6 0.015781239933471813 7 4.394664420348597E-6 8 0.0 9 7.339089581982158E-4 10-11 8.525648975476278E-4 12-13 5.273597304418316E-5 14-15 3.076265094244018E-4 16-17 1.7578657681394387E-4 18-19 2.1973322101742985E-6 20-21 8.789328840697194E-6 22-23 1.318399326104579E-5 24-25 0.0 26-27 5.119784049706115E-4 28-29 0.006517287335376969 30-31 0.0016633804831019442 32-33 0.20627675856232244 34-35 0.008040038557027758 36-37 0.006253607470156054 38-39 9.096955350121595E-4 40-41 8.525648975476278E-4 42-43 0.06084193156751615 44-45 0.3985674976068855 46-47 0.11572030351661926 48-49 0.1680585594307609 50-51 0.002302804156262665 52-53 0.0026829426286228185 54-55 8.152102499746647E-4 56-57 2.94442516163356E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.007128145689805424 82-83 0.00773460937981353 84-85 0.007466534850172267 86-87 0.00613934619522699 88-89 0.012808249453105986 90-91 0.012524793597993501 92-93 0.01956724333160213 94-95 0.015726306628217453 96-97 0.007108369699913857 98-99 0.007642321426986211 100-101 0.007684070738979522 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.2754866E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.71034626116037 #Duplication Level Percentage of deduplicated Percentage of total 1 74.81996671373908 37.94146419302202 2 13.470607794503122 13.661983712150782 3 4.571199055822957 6.954212608484143 4 2.1253794189067645 4.311149050764234 5 1.22666295355576 3.110225156027514 6 0.7741251759575809 2.3553693433374376 7 0.5136598802137771 1.8233509270274753 8 0.3733869961157028 1.5147667089953465 9 0.2983185083477286 1.361505136898356 >10 1.634673426691283 15.493916281187747 >50 0.13128792782501805 4.564088877606217 >100 0.0592508288368225 5.174790994744913 >500 0.0013246199583182255 0.4394062409940023 >1k 1.2196280499352329E-4 0.09802617088759964 >5k 8.684180270943457E-6 0.03682439067720306 >10k+ 2.605254081283037E-5 1.1589202071951858 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC 226651 0.9960550855364299 TruSeq Adapter, Index 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.11061370346017418 4.394664420348597E-6 0.0 0.0 0.0 2 0.11125532446554508 3.0762650942440176E-5 0.0 3.0762650942440176E-5 0.0 3 0.11129927110974858 3.0762650942440176E-5 0.0 3.0762650942440176E-5 0.0 4 0.11158492429707123 3.0762650942440176E-5 0.0 3.9551979783137375E-5 4.394664420348597E-6 5 0.11163326560569506 3.5157315362788776E-5 0.0 3.9551979783137375E-5 4.394664420348597E-6 6 0.11174752688062413 3.5157315362788776E-5 0.0 4.394664420348597E-5 4.394664420348597E-6 7 0.11193210278627877 5.273597304418317E-5 0.0 4.834130862383457E-5 4.394664420348597E-6 8 0.1123408065773712 6.152530188488035E-5 0.0 5.273597304418317E-5 4.394664420348597E-6 9 0.11248143583882234 6.152530188488035E-5 4.394664420348597E-6 6.152530188488035E-5 4.394664420348597E-6 10-11 0.11280664100592813 6.591996630522895E-5 4.394664420348597E-6 7.031463072557755E-5 4.394664420348597E-6 12-13 0.11322413412586126 7.251196293575184E-5 4.394664420348597E-6 7.470929514592615E-5 4.394664420348597E-6 14-15 0.11381741382260832 9.448528503749483E-5 4.394664420348597E-6 7.910395956627475E-5 4.394664420348597E-6 16-17 0.1145623094418574 1.0107728166801773E-4 4.394664420348597E-6 8.569595619679764E-5 8.789328840697194E-6 18-19 0.11529841573226579 1.0547194608836633E-4 4.394664420348597E-6 9.448528503749483E-5 8.789328840697194E-6 20-21 0.11801871300846158 1.1206394271888923E-4 4.394664420348597E-6 1.0107728166801773E-4 1.0986661050871492E-5 22-23 0.12056761837226376 1.340372648206322E-4 4.394664420348597E-6 1.0327461387819203E-4 1.3183993261045792E-5 24-25 0.12151906321926922 1.560105869223752E-4 4.394664420348597E-6 1.1865593934941212E-4 1.3183993261045792E-5 26-27 0.12248369205953574 1.647999157630724E-4 4.394664420348597E-6 1.230506037697607E-4 1.3183993261045792E-5 28-29 0.12368343544629092 1.69194580183421E-4 4.394664420348597E-6 1.25247935979935E-4 1.538132547122009E-5 30-31 0.12486779750757486 1.8018124123429249E-4 4.394664420348597E-6 1.2744526819010932E-4 1.7578657681394388E-5 32-33 0.12628947144755764 1.9336523449533827E-4 4.394664420348597E-6 1.2744526819010932E-4 2.1973322101742984E-5 34-35 0.1276276467635538 1.9556256670551258E-4 4.394664420348597E-6 1.2744526819010932E-4 2.1973322101742984E-5 36-37 0.1291042540087909 2.0874655996655836E-4 4.394664420348597E-6 1.318399326104579E-4 2.1973322101742984E-5 38-39 0.13075445049863182 2.1533855659708125E-4 4.394664420348597E-6 1.318399326104579E-4 2.1973322101742984E-5 40-41 0.13258043356528665 2.1533855659708125E-4 4.394664420348597E-6 1.318399326104579E-4 2.1973322101742984E-5 42-43 0.1341581180921918 2.3731187869882424E-4 6.591996630522896E-6 1.3623459703080652E-4 2.1973322101742984E-5 44-45 0.13575338127677833 2.5269320417004433E-4 8.789328840697194E-6 1.47221258081678E-4 2.1973322101742984E-5 46-47 0.1377221909370945 2.7466652627178734E-4 8.789328840697194E-6 1.560105869223752E-4 2.1973322101742984E-5 48-49 0.13969100059741069 2.966398483735303E-4 8.789328840697194E-6 1.647999157630724E-4 2.1973322101742984E-5 50-51 0.14188174081095445 2.988371805837046E-4 8.789328840697194E-6 1.669972479732467E-4 2.1973322101742984E-5 52-53 0.14436033154403105 3.032318450040532E-4 8.789328840697194E-6 1.7139191239359529E-4 2.4170654311917284E-5 54-55 0.14694439422319605 3.054291772142275E-4 8.789328840697194E-6 1.7139191239359529E-4 2.6367986522091584E-5 56-57 0.1495811928754052 3.142185060549247E-4 8.789328840697194E-6 1.7578657681394387E-4 2.6367986522091584E-5 58-59 0.15314966038472827 3.339944959464934E-4 8.789328840697194E-6 1.7798390902411818E-4 2.6367986522091584E-5 60-61 0.15732239425184924 3.339944959464934E-4 8.789328840697194E-6 1.8897057007498968E-4 2.6367986522091584E-5 62-63 0.16380012960744308 3.449811569973649E-4 8.789328840697194E-6 2.0654922775638406E-4 2.6367986522091584E-5 64-65 0.1736837298888071 3.5596781804823636E-4 8.789328840697194E-6 2.2193055322760415E-4 2.6367986522091584E-5 66-67 0.17880790860293355 3.7354647572963076E-4 8.789328840697194E-6 2.3071988206830135E-4 2.856531873226588E-5 68-69 0.18381562870992077 3.8233580457032793E-4 8.789328840697194E-6 2.482985397496957E-4 3.0762650942440176E-5 70-71 0.1907811718161733 3.999144622517223E-4 8.789328840697194E-6 2.614825330107415E-4 3.0762650942440176E-5 72-73 0.2008339666777207 4.0211179446189663E-4 8.789328840697194E-6 2.7246919406161303E-4 3.0762650942440176E-5 74-75 0.21590107364288585 4.1969045214329103E-4 8.789328840697194E-6 2.790611906921359E-4 3.0762650942440176E-5 76-77 0.23366210989772473 4.262824487738139E-4 8.789328840697194E-6 2.812585229023102E-4 3.0762650942440176E-5 78-79 0.2572702471638374 4.262824487738139E-4 8.789328840697194E-6 2.856531873226588E-4 3.0762650942440176E-5 80-81 0.2846555984992397 4.284797809839882E-4 8.789328840697194E-6 3.010345127938789E-4 3.0762650942440176E-5 82-83 0.32551718827964093 4.328744454043368E-4 8.789328840697194E-6 3.032318450040532E-4 3.0762650942440176E-5 84-85 0.38715894877166057 4.438611064552083E-4 8.789328840697194E-6 3.120211738447504E-4 3.0762650942440176E-5 86-87 0.4566803425693652 4.460584386653826E-4 8.789328840697194E-6 3.295998315261448E-4 3.2959983152614476E-5 88-89 0.5274871757100217 4.548477675060798E-4 8.789328840697194E-6 3.4937582141771344E-4 3.9551979783137375E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 50630 0.0 50.30448 3 ATCGGAA 51120 0.0 50.074024 2 GATCGGA 51520 0.0 49.79217 1 CGGAAGA 52550 0.0 48.512016 4 AGAGCAC 54835 0.0 46.60402 8 GAGCACA 56350 0.0 45.578575 9 AAGAGCA 64100 0.0 39.949074 7 CGTATGC 35660 0.0 38.441544 44-45 CTCGTAT 32350 0.0 38.224503 42-43 TATGCCG 35990 0.0 38.019737 46-47 GAAGAGC 68800 0.0 37.274597 6 CACGATC 34010 0.0 36.169685 36-37 TCGTATG 34950 0.0 36.0495 42-43 ATCTCGT 34385 0.0 35.762474 40-41 ATCACGA 39050 0.0 35.571365 34-35 TCTCGTA 33345 0.0 34.22638 40-41 CCGTCTT 40185 0.0 33.983215 50-51 TGCCGTC 40430 0.0 33.745216 48-49 ACGATCT 33890 0.0 33.69805 36-37 TCACGAT 37815 0.0 33.199135 34-35 >>END_MODULE