FastQCFastQC Report
Wed 25 May 2016
SRR1033290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11407454
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT13750.011.3603011
CGTATCG10750.010.5627183
CTCCGAT23750.09.9651771
GATCGGA85750.09.63936194
CGGGGTT36000.08.6780071
CTCGCGT10608.067218E-98.4845021
CCCGGTT27800.08.3431471
CGTCGAT13301.05501385E-108.185681
CTCGGAT31250.08.1794161
CGCCGAT18000.08.1520671
CGCCAAT22700.08.1323731
CGCGGAT12904.838512E-108.0725651
TAGATCG18050.08.06592694
AGATCGG104550.08.04079194
CTCGACT19200.07.8890981
TACCGTA7905.1184477E-57.79644979
CCCCGTT23100.07.78664161
CGCGTAC6100.00206083557.7597681
CTCCGTT27650.07.703641
CGCCATT26200.07.58798741
Produced by FastQC (version 0.11.5)