##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033290_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11407454
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.331110342412956	34.0	31.0	34.0	31.0	34.0
2	32.504844726965366	34.0	31.0	34.0	31.0	34.0
3	32.57179866778336	34.0	31.0	34.0	31.0	34.0
4	35.87504968242695	37.0	37.0	37.0	35.0	37.0
5	35.85498727411042	37.0	37.0	37.0	35.0	37.0
6	35.79278908334848	37.0	37.0	37.0	35.0	37.0
7	35.752081577536934	37.0	37.0	37.0	35.0	37.0
8	35.7390267802088	37.0	37.0	37.0	35.0	37.0
9	37.51616285281536	39.0	39.0	39.0	35.0	39.0
10-11	37.634241917609316	39.0	39.0	39.0	35.0	39.0
12-13	37.576236774656294	39.0	39.0	39.0	35.0	39.0
14-15	39.08130153319049	41.0	40.0	41.0	36.0	41.0
16-17	39.17766374512665	41.0	40.0	41.0	36.0	41.0
18-19	39.224381663077494	41.0	40.0	41.0	36.0	41.0
20-21	39.187978930267874	41.0	40.0	41.0	36.0	41.0
22-23	39.12579651866227	41.0	39.5	41.0	36.0	41.0
24-25	39.07945361866022	41.0	39.0	41.0	36.0	41.0
26-27	39.00610272020383	41.0	39.0	41.0	36.0	41.0
28-29	38.91002606716626	41.0	39.0	41.0	35.0	41.0
30-31	38.90384287326515	41.0	39.0	41.0	35.0	41.0
32-33	38.9839843754794	41.0	39.0	41.0	35.0	41.0
34-35	38.91775474176797	41.0	39.0	41.0	35.0	41.0
36-37	38.858277315867326	41.0	39.0	41.0	35.0	41.0
38-39	38.770365411949065	41.0	39.0	41.0	35.0	41.0
40-41	38.63669741732029	41.0	39.0	41.0	35.0	41.0
42-43	38.51661496947522	41.0	38.5	41.0	34.5	41.0
44-45	38.38162761822226	41.0	38.0	41.0	34.0	41.0
46-47	38.251585235408356	40.0	38.0	41.0	34.0	41.0
48-49	38.106063368741175	40.0	38.0	41.0	34.0	41.0
50-51	37.92355248594472	40.0	37.0	41.0	33.0	41.0
52-53	37.7347497960544	40.0	37.0	41.0	33.0	41.0
54-55	37.52873572841057	40.0	36.0	41.0	33.0	41.0
56-57	37.291336962656175	39.5	35.5	41.0	33.0	41.0
58-59	37.04511773617496	39.0	35.0	41.0	32.0	41.0
60-61	36.77632953856312	39.0	35.0	41.0	32.0	41.0
62-63	36.4822724685105	38.5	35.0	41.0	32.0	41.0
64-65	36.204007967071355	37.5	35.0	40.5	31.5	41.0
66-67	35.88589430209405	37.0	35.0	40.0	31.0	41.0
68-69	35.56568674307168	36.5	35.0	39.5	31.0	41.0
70-71	35.23896278696368	36.0	35.0	39.0	31.0	41.0
72-73	34.89131005919463	35.0	35.0	39.0	30.5	41.0
74-75	34.56103890491252	35.0	35.0	37.5	30.0	40.0
76-77	34.31070162544596	35.0	34.5	37.0	30.0	39.0
78-79	34.146387835532806	35.0	35.0	37.0	31.0	39.0
80-81	33.89242108712426	35.0	34.5	36.0	30.0	38.5
82-83	33.66370396935197	35.0	34.0	36.0	30.0	37.0
84-85	33.45081277557639	35.0	34.0	35.5	30.0	37.0
86-87	33.263315591717486	35.0	34.0	35.0	30.0	36.5
88-89	33.09242873124889	35.0	34.0	35.0	30.0	36.0
90-91	32.96951962287115	35.0	34.0	35.0	30.0	36.0
92-93	32.82574792762697	35.0	34.0	35.0	29.0	36.0
94-95	32.704428569249544	35.0	34.0	35.0	29.0	36.0
96-97	32.62977882707219	35.0	34.0	35.0	29.0	35.0
98-99	32.54122436084336	35.0	34.0	35.0	29.0	35.0
100	32.451262569193794	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-6.136846177824395E-4
1101	2	-0.03995084522821202
1101	3	-0.010190824387947828
1101	4	-0.017724898704187808
1101	5	0.007217231483707565
1101	6	0.013645401333818086
1101	7	0.12119066069676876
1101	8	0.11843778863664767
1101	9	0.10387082386222346
1101	10-11	0.14303724221082348
1101	12-13	0.09031145732739532
1101	14-15	0.18592191709034722
1101	16-17	0.201761565645576
1101	18-19	0.17488425827025367
1101	20-21	0.22308976001479408
1101	22-23	0.2608197143958577
1101	24-25	0.2182694639092162
1101	26-27	0.09753188714670813
1101	28-29	0.07769259876015155
1101	30-31	0.1611887802877945
1101	32-33	0.14060096841939895
1101	34-35	0.17302884182591072
1101	36-37	0.1530496238546064
1101	38-39	0.17587346474195442
1101	40-41	0.1269119369854934
1101	42-43	0.11119090399107989
1101	44-45	0.10365637885330159
1101	46-47	0.017441713050288854
1101	48-49	0.05525238104053187
1101	50-51	0.0894612103023178
1101	52-53	0.06977192208899652
1101	54-55	0.10220524939319375
1101	56-57	0.1181777672320834
1101	58-59	0.11920678075863123
1101	60-61	0.08750516822740906
1101	62-63	0.12451944908158197
1101	64-65	0.09666701720204429
1101	66-67	0.012000174210889725
1101	68-69	0.09102762024834732
1101	70-71	0.10824054832380625
1101	72-73	0.10801354200265934
1101	74-75	0.04456972966944761
1101	76-77	0.07492449322170103
1101	78-79	0.11359171151207192
1101	80-81	0.11193636763115933
1101	82-83	0.14280194905301613
1101	84-85	0.16320775361463546
1101	86-87	0.12149964872679675
1101	88-89	0.17200443895633555
1101	90-91	0.16254652983036522
1101	92-93	0.1620199359486989
1101	94-95	0.14870212961172058
1101	96-97	0.10738611891780181
1101	98-99	0.0774299019508291
1101	100	0.10426325061100528
1102	1	0.09640072664836907
1102	2	0.07165812135669114
1102	3	0.09806390252753516
1102	4	0.06315113020920649
1102	5	0.09976707603254908
1102	6	0.128099276264102
1102	7	0.1768134227187943
1102	8	0.18596297897207137
1102	9	0.20814297417489058
1102	10-11	0.2797244774122163
1102	12-13	0.17318228027718874
1102	14-15	0.3553676252436162
1102	16-17	0.339971767436559
1102	18-19	0.3581031778023771
1102	20-21	0.3021735655961706
1102	22-23	0.3361878835130767
1102	24-25	0.3440909201559492
1102	26-27	0.3584964718947745
1102	28-29	0.342146854212416
1102	30-31	0.37761594183458413
1102	32-33	0.3913969882686317
1102	34-35	0.3938538157870255
1102	36-37	0.37926457947508396
1102	38-39	0.378975757015219
1102	40-41	0.3776235527415821
1102	42-43	0.4015176730206278
1102	44-45	0.38811863240211153
1102	46-47	0.3674781339372757
1102	48-49	0.3804821066572259
1102	50-51	0.38126935275411
1102	52-53	0.3557349913554049
1102	54-55	0.32695209040446827
1102	56-57	0.33321037955199984
1102	58-59	0.3100078101093686
1102	60-61	0.3373476457771858
1102	62-63	0.3467456380106384
1102	64-65	0.2770842224790826
1102	66-67	0.30742061982645197
1102	68-69	0.2416157244685948
1102	70-71	0.24228912778677625
1102	72-73	0.2563567008189196
1102	74-75	0.23830274039764276
1102	76-77	0.2860738808517169
1102	78-79	0.3089712549776422
1102	80-81	0.31522917193746025
1102	82-83	0.3201342660931559
1102	84-85	0.349585298529405
1102	86-87	0.3017807006819453
1102	88-89	0.32421701092366106
1102	90-91	0.279649826540755
1102	92-93	0.29174999162417237
1102	94-95	0.37184258647876334
1102	96-97	0.3704901158767342
1102	98-99	0.3576743841573844
1102	100	0.37687953095183957
1103	1	-0.02959424024052737
1103	2	-0.011305302660083782
1103	3	0.013567136413612957
1103	4	-0.011716397455316496
1103	5	-0.008188614237027991
1103	6	0.0808772473121877
1103	7	0.108942477414665
1103	8	0.13031807048434985
1103	9	0.17585854775581566
1103	10-11	0.16568150806381965
1103	12-13	0.1050745557818118
1103	14-15	0.27418689347039304
1103	16-17	0.241726539456657
1103	18-19	0.25548432649614483
1103	20-21	0.2393426866113444
1103	22-23	0.25131783109263495
1103	24-25	0.2350491157118597
1103	26-27	0.2180731487847325
1103	28-29	0.21031286417087358
1103	30-31	0.20152842907056367
1103	32-33	0.2217426790961312
1103	34-35	0.25522621494506836
1103	36-37	0.1982635714844534
1103	38-39	0.2321083698636599
1103	40-41	0.2643388088225649
1103	42-43	0.22511351556742198
1103	44-45	0.23339051820235568
1103	46-47	0.19830473454624098
1103	48-49	0.2827434774791939
1103	50-51	0.25862815193722355
1103	52-53	0.2762611550661447
1103	54-55	0.25900954914015983
1103	56-57	0.26184694117704765
1103	58-59	0.2934571697742214
1103	60-61	0.2704920802285784
1103	62-63	0.2615172536265291
1103	64-65	0.24542575919389265
1103	66-67	0.2514527340072945
1103	68-69	0.21581870956169524
1103	70-71	0.23129456035114515
1103	72-73	0.23217108867264358
1103	74-75	0.2149411389543019
1103	76-77	0.261765565115212
1103	78-79	0.29906873817557766
1103	80-81	0.26457902024834823
1103	82-83	0.3001350335565718
1103	84-85	0.28800823868752445
1103	86-87	0.29896698259160814
1103	88-89	0.3042927387599548
1103	90-91	0.2939946447087749
1103	92-93	0.3408972758777793
1103	94-95	0.32654718374811864
1103	96-97	0.3081597986167637
1103	98-99	0.29946652266564655
1103	100	0.32161126060609746
1104	1	-0.09781576802532044
1104	2	-0.06331167386437642
1104	3	-0.04779478414084082
1104	4	-0.019689325808478486
1104	5	-0.01194626728820225
1104	6	0.014766708317573318
1104	7	0.06276845374858198
1104	8	0.04663081247891654
1104	9	0.06696560700701326
1104	10-11	0.13181789721203074
1104	12-13	0.043466984075379855
1104	14-15	0.16044370983106404
1104	16-17	0.1546569379181122
1104	18-19	0.18038501822708497
1104	20-21	0.20318479029757697
1104	22-23	0.21773942246515077
1104	24-25	0.1588106108672065
1104	26-27	0.17592511225733887
1104	28-29	0.1529513966406455
1104	30-31	0.15030922419746418
1104	32-33	0.12064809021892131
1104	34-35	0.1488458194137081
1104	36-37	0.16639497872353815
1104	38-39	0.2033016862729511
1104	40-41	0.15190478214154268
1104	42-43	0.19434462252234397
1104	44-45	0.208361823395407
1104	46-47	0.1485528547790267
1104	48-49	0.09802936015140062
1104	50-51	0.13972734020779143
1104	52-53	0.16886895443720817
1104	54-55	0.15971171098475168
1104	56-57	0.14948739853393533
1104	58-59	0.18783505130465983
1104	60-61	0.1498356088878694
1104	62-63	0.15985413966937045
1104	64-65	0.11858280381231623
1104	66-67	0.11219791203023277
1104	68-69	0.1096078728385379
1104	70-71	0.08077622826849051
1104	72-73	0.09482850221153427
1104	74-75	0.12163878396121675
1104	76-77	0.08567635241699634
1104	78-79	0.12972349531522553
1104	80-81	0.15733494037304752
1104	82-83	0.13024962480731972
1104	84-85	0.14822641041786255
1104	86-87	0.1562769257042902
1104	88-89	0.15635796030537108
1104	90-91	0.1350266972728491
1104	92-93	0.18216654050576864
1104	94-95	0.1967727546090572
1104	96-97	0.10278317950402993
1104	98-99	0.10442268845996239
1104	100	0.13309626876390723
1105	1	-0.08466695564935378
1105	2	-0.09050146001363402
1105	3	-0.07121034914685254
1105	4	-0.06258411585704948
1105	5	-0.01768519103934807
1105	6	0.048439410483013035
1105	7	0.06480832734096253
1105	8	0.04493114630376738
1105	9	0.03941003583045699
1105	10-11	0.09283251006191051
1105	12-13	-0.005260020410190691
1105	14-15	0.10956670253761303
1105	16-17	0.15996690590225882
1105	18-19	0.1429613163944765
1105	20-21	0.13344041197025547
1105	22-23	0.1393600059387765
1105	24-25	0.11258918493935255
1105	26-27	0.13342160656291213
1105	28-29	0.1573889363268961
1105	30-31	0.16202105642508258
1105	32-33	0.10504119619685781
1105	34-35	0.12803727656221042
1105	36-37	0.07768489418216973
1105	38-39	0.09307088397054031
1105	40-41	0.12193911693263715
1105	42-43	0.08136853653379461
1105	44-45	0.07672055665499045
1105	46-47	0.025546912639676123
1105	48-49	0.03252836194106834
1105	50-51	0.029289688817108583
1105	52-53	0.04888473694531825
1105	54-55	0.09344852443413743
1105	56-57	0.07265927856900589
1105	58-59	0.07451334490062322
1105	60-61	0.08940885834009293
1105	62-63	0.03906218445934684
1105	64-65	0.04476106880245112
1105	66-67	-0.0012200350005073801
1105	68-69	-3.996886331307792E-4
1105	70-71	0.06511109153088768
1105	72-73	0.052346518674525555
1105	74-75	0.05391204488249457
1105	76-77	0.057308146047702735
1105	78-79	0.011128772516144636
1105	80-81	0.0673932412091034
1105	82-83	0.038812042145494274
1105	84-85	0.044789594916316844
1105	86-87	0.037656728399873884
1105	88-89	0.054171805160962094
1105	90-91	0.08437733376392487
1105	92-93	0.10532640502126611
1105	94-95	0.09681980969781279
1105	96-97	0.0801902833083119
1105	98-99	0.08369245746848009
1105	100	0.11339965694796206
1106	1	-0.12841093309510399
1106	2	-0.10688109446677174
1106	3	-0.06597475411586373
1106	4	-0.08887534966910948
1106	5	-0.12008812688190318
1106	6	-0.02489544153134915
1106	7	-0.00974909288164838
1106	8	-5.445814923135117E-4
1106	9	0.019021892909037774
1106	10-11	0.07304903431796106
1106	12-13	-0.016340631558101393
1106	14-15	0.07850538733286072
1106	16-17	0.09338966517908176
1106	18-19	0.07148169857359221
1106	20-21	0.07222638302362583
1106	22-23	0.0877078375151612
1106	24-25	0.04116411928883679
1106	26-27	0.07675454116752434
1106	28-29	0.030289895689179502
1106	30-31	0.06750206555651062
1106	32-33	0.06337829571781839
1106	34-35	0.090959820596197
1106	36-37	0.06409304694674489
1106	38-39	0.12429993505378434
1106	40-41	0.0981462065648202
1106	42-43	0.07836425910828382
1106	44-45	0.07908042786861103
1106	46-47	0.11947496805962743
1106	48-49	0.09518490766508592
1106	50-51	0.12090715199578739
1106	52-53	0.09566862471607607
1106	54-55	0.10829448805641562
1106	56-57	0.07268032317957562
1106	58-59	0.10136474224028547
1106	60-61	0.05577841964662866
1106	62-63	0.08979594916062439
1106	64-65	0.07480764939639784
1106	66-67	0.06014569609055087
1106	68-69	0.07622261421709453
1106	70-71	0.06616961777601205
1106	72-73	0.057085175019125245
1106	74-75	0.10207939496633145
1106	76-77	0.09989206811084017
1106	78-79	0.10818075074487155
1106	80-81	0.05419491373689311
1106	82-83	0.10669413438001385
1106	84-85	0.1175275905104769
1106	86-87	0.12519912303821457
1106	88-89	0.12473169694487751
1106	90-91	0.08917927919175384
1106	92-93	0.0945751947607576
1106	94-95	0.09374675585104342
1106	96-97	0.08456892209601108
1106	98-99	0.05999460385601907
1106	100	0.04925944208800104
1107	1	-0.1323446157693411
1107	2	-0.10274228647597283
1107	3	-0.04943806330486211
1107	4	-0.037367325291960185
1107	5	-0.01822726159768706
1107	6	0.044813615124276396
1107	7	0.02758242482593687
1107	8	0.0670527236989642
1107	9	0.030741995099262454
1107	10-11	0.09597221897105612
1107	12-13	0.0155971664746275
1107	14-15	0.14395695788186202
1107	16-17	0.029922007965943465
1107	18-19	0.09726941920673227
1107	20-21	0.09485060918724031
1107	22-23	0.03604376625801109
1107	24-25	0.06644626686004074
1107	26-27	0.09691865395129895
1107	28-29	0.09515473459292423
1107	30-31	0.08347607896912024
1107	32-33	0.051806701991900184
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2216	22-23	-0.33233298182811666
2216	24-25	-0.27454963029478563
2216	26-27	-0.31907231394951197
2216	28-29	-0.30630592607784024
2216	30-31	-0.29975920470491957
2216	32-33	-0.30961421359099006
2216	34-35	-0.3493071109136068
2216	36-37	-0.3583885799853661
2216	38-39	-0.2467017944947969
2216	40-41	-0.5690611652100799
2216	42-43	-0.7607173846352495
2216	44-45	-0.479845007061769
2216	46-47	-0.3399928600014519
2216	48-49	-0.43567405450221486
2216	50-51	-0.4190252586232859
2216	52-53	-0.3554543432920667
2216	54-55	-0.47185296908928365
2216	56-57	-0.3392873416495732
2216	58-59	-0.45692384648315
2216	60-61	-0.537227223743983
2216	62-63	-0.44971935853272527
2216	64-65	-0.44314998256114535
2216	66-67	-0.41674347547520085
2216	68-69	-0.4419838249349439
2216	70-71	-0.4357283780896921
2216	72-73	-0.51315320794032
2216	74-75	-0.4115657669056816
2216	76-77	-0.45485580473508946
2216	78-79	-0.5022084986632365
2216	80-81	-0.4653947044629021
2216	82-83	-0.6100524782542038
2216	84-85	-0.47414258463253134
2216	86-87	-0.37741204320804655
2216	88-89	-0.41960438666910704
2216	90-91	-0.5480489281650733
2216	92-93	-0.8534946643141055
2216	94-95	-0.7594774129434292
2216	96-97	-0.7199216024129313
2216	98-99	-0.5647304794851138
2216	100	-0.498159290852513
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47756.0
3	8673.0
4	6838.0
5	6671.0
6	8525.0
7	10973.0
8	11849.0
9	11512.0
10	13818.0
11	13169.0
12	13640.0
13	14782.0
14	15297.0
15	15985.0
16	16562.0
17	17139.0
18	17026.0
19	16162.0
20	16757.0
21	17587.0
22	18985.0
23	23244.0
24	31042.0
25	47930.0
26	64026.0
27	69687.0
28	77843.0
29	85751.0
30	107182.0
31	136517.0
32	178899.0
33	241260.0
34	349773.0
35	585998.0
36	1254379.0
37	2730761.0
38	3700542.0
39	1397919.0
40	4995.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.945172534863815	12.416012367936217	11.53381897277144	50.104996124428524
2	21.12825896695061	19.55433923252159	36.0966487455134	23.220753055014402
3	25.68434195575869	22.38157988068442	23.55860196080764	28.375476202749255
4	27.271195043625717	29.31930557967435	18.562976390191743	24.84652298650819
5	28.534611721967746	30.852936519189	20.978418820609292	19.63403293823396
6	22.906219596985274	33.2568384873535	22.052343560843468	21.784598354817764
7	20.370590322995717	17.683327960204913	38.830856745326436	23.11522497147293
8	21.149615199815095	21.433942384397692	28.906585309738553	28.509857106048663
9	21.466697447491274	20.7148846515133	31.123606238954725	26.694811662040696
10-11	25.297602364926732	29.588194303222092	21.40815621300567	23.706047118845504
12-13	23.646581125653654	23.890795414805048	26.970268354763576	25.49235510477772
14-15	24.060051199691742	25.115207231249826	26.285924910112602	24.538816658945827
16-17	24.93970527136925	25.188061503429243	25.178341214498584	24.69389201070293
18-19	24.658909403369293	25.322560984776878	25.074779201758783	24.94375041009505
20-21	24.55941160385597	25.441897090826526	25.407309846772243	24.591381458545268
22-23	24.765723359480564	25.516556981075706	25.350665450853448	24.36705420859028
24-25	24.562711363990598	25.6275852613563	25.351016098771908	24.458687275881193
26-27	24.369405077792226	25.714104118783247	25.435525346552318	24.480965456872212
28-29	24.53889365687908	25.377283570565623	25.334540422170456	24.749282350384842
30-31	24.400001139634355	25.44270082822927	25.397571307688782	24.75972672444759
32-33	24.359602940323054	25.67692142348328	25.516662175451245	24.446813460742423
34-35	24.72774509518486	25.539313937984883	25.130734269180703	24.602206697649553
36-37	24.51686528138812	25.425821190734567	25.229593802424716	24.8277197254526
38-39	24.446584843664894	25.5844325831669	25.407967270565507	24.5610153026027
40-41	24.706067789400276	25.426429064432977	25.274376588578896	24.59312655758785
42-43	24.44075766589626	25.38096077946191	25.494793174794545	24.683488379847287
44-45	24.458644511123797	25.503765012943052	25.553316892361583	24.484273583571568
46-47	24.57898691834162	25.5025139124525	25.26174081810583	24.656758351100045
48-49	24.51821621083091	25.356229923159866	25.405855193531306	24.719698672477914
50-51	24.516191156790708	25.514168564560524	25.509185016629736	24.460455262019025
52-53	24.568350984876822	25.534748427783306	25.267361055064253	24.629539532275622
54-55	24.390466424490533	25.417566884228478	25.374323967384466	24.817642723896522
56-57	24.415750248935172	25.503090305345854	25.52011001399301	24.561049431725966
58-59	24.620427248475522	25.415781662329906	25.35112508671624	24.612666002478335
60-61	24.434005890645842	25.493174049225875	25.434852170121182	24.637967890007104
62-63	24.332713511000126	25.627312206695397	25.5729830042135	24.466991278090976
64-65	24.495662192908156	25.51572106128331	25.365302464094068	24.623314281714467
66-67	24.445736777710486	25.44624573262817	25.430840667809562	24.677176821851788
68-69	24.463564306925697	25.64012159121315	25.517442549029067	24.378871552832084
70-71	24.573490222407138	25.51857707346972	25.328325854605716	24.579606849517425
72-73	24.426362951626228	25.528269805742404	25.406559446220495	24.63880779641087
74-75	24.40647389379989	25.666464789801662	25.48090287741054	24.44615843898791
76-77	24.630192961107454	25.509886610112133	25.33612007179119	24.523800356989224
78-79	24.48308082385116	25.369585345112434	25.531377609830663	24.61595622120574
80-81	24.409539796136695	25.61153152792473	25.584244163265186	24.394684512673393
82-83	24.526307240466572	25.519462624099997	25.393492935013462	24.560737200419968
84-85	24.482027004232528	25.462193771443648	25.513372851101156	24.54240637322267
86-87	24.483042384958253	25.651088285941903	25.596454086303066	24.26941524279678
88-89	24.595404118475784	25.575792790813484	25.32668177252152	24.502121318189214
90-91	24.464144919834446	25.56032274772762	25.375666982476147	24.59986534996178
92-93	24.432312633980594	25.721911733175222	25.556712269411552	24.28906336343263
94-95	24.75333408614206	25.664613002697866	25.25168439490263	24.33036851625744
96-97	24.623184550207615	25.59368567685267	25.363474149292863	24.419655623646854
98-99	24.65016990204283	25.76617969143597	25.388511270239107	24.195139136282094
100	24.85881629509548	25.539264019213714	25.051597962897937	24.55032172279287
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	26.5
2	20.0
3	37.0
4	59.0
5	91.0
6	144.0
7	223.5
8	313.5
9	419.5
10	536.0
11	633.0
12	756.5
13	859.5
14	958.0
15	1100.5
16	1322.5
17	1686.0
18	2206.5
19	3036.0
20	4524.5
21	6837.5
22	10337.0
23	15428.5
24	22303.5
25	31322.5
26	42657.0
27	55879.5
28	70130.5
29	84520.0
30	99652.5
31	115852.5
32	132327.5
33	149238.5
34	166118.5
35	185237.0
36	206474.0
37	229564.0
38	249359.5
39	266459.0
40	283068.5
41	297265.5
42	313112.5
43	330330.0
44	341488.0
45	350870.5
46	362846.5
47	366910.5
48	369988.0
49	374184.5
50	378118.5
51	380103.0
52	373925.5
53	369714.5
54	365227.0
55	359010.5
56	347442.5
57	330251.0
58	317992.0
59	306751.5
60	295090.0
61	281255.5
62	263099.5
63	244850.5
64	226200.5
65	203790.5
66	175517.0
67	150372.5
68	129199.5
69	104403.5
70	79906.0
71	58774.5
72	42073.0
73	28791.5
74	18849.0
75	11599.0
76	6819.0
77	4068.5
78	2273.5
79	1069.5
80	597.0
81	394.5
82	284.0
83	208.0
84	138.0
85	96.0
86	74.5
87	66.0
88	55.5
89	40.5
90	32.0
91	28.0
92	26.0
93	20.0
94	16.5
95	15.5
96	13.5
97	10.0
98	10.0
99	14.0
100	24.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.6794855363869975
2	0.5191868404641387
3	0.5133397864238594
4	0.503030737621208
5	0.5172144459228151
6	0.7232814614023427
7	0.8054908658847102
8	0.8586315579269486
9	0.8916450594497247
10-11	0.3802864337651504
12-13	0.6232635257613136
14-15	0.4448407155531813
16-17	0.030436239322113416
18-19	3.2873242355393236E-4
20-21	0.02595232906483778
22-23	0.0
24-25	0.0
26-27	0.00549202302284103
28-29	0.008305972568462692
30-31	0.0025115157159520435
32-33	0.0
34-35	0.036555045499197275
36-37	2.1915494903595493E-4
38-39	0.008441848636864983
40-41	0.1142454749324433
42-43	0.09532363663267895
44-45	0.07267178110032264
46-47	0.042161029095537005
48-49	0.17629262410350285
50-51	0.08723681901325221
52-53	0.07242632755740239
54-55	0.11992597121145525
56-57	0.0780542266486457
58-59	0.09719960299642671
60-61	0.1283853522442431
62-63	0.15455683625811684
64-65	0.060797965961554616
66-67	0.0754375165571564
68-69	0.07542436726021425
70-71	0.036033456720491706
72-73	0.0724087951614795
74-75	0.17663450582399892
76-77	0.15809399713555713
78-79	0.1266671774438012
80-81	0.1539957995885848
82-83	0.10392765993183055
84-85	0.19235229876885762
86-87	0.19047633240510986
88-89	0.10987990834764706
90-91	0.08555370900465607
92-93	0.1780458636957905
94-95	0.12767529020936663
96-97	0.17430269716625638
98-99	0.193469989008941
100	0.165882764024295
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1407454E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.825179567960205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.21264055475886	48.78948265363455
2	11.108395941624902	13.513403557226699
3	3.556999305787783	6.490653644929551
4	1.5514502455227244	3.7746895909870264
5	0.8872191778239086	2.698263290363863
6	0.5731659550744178	2.0917753283785725
7	0.37007942513077885	1.5757103241590977
8	0.2580646528919072	1.2557463081834863
9	0.20543443914317686	1.1246027985294234
>10	1.1756208653981353	12.978808034844459
>50	0.07478864201927178	3.1074390669207093
>100	0.025923015921564475	2.520188481191566
>500	2.0334789314662566E-4	0.06985357284206248
>1k	1.4431009397650214E-5	0.009383347808781663
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.010177555833229747	8.766197961438197E-6	0.0	0.0	0.0
2	0.010230153020998375	4.383098980719098E-5	0.0	3.506479184575279E-5	0.0
3	0.010335347396535634	4.383098980719098E-5	0.0	3.506479184575279E-5	0.0
4	0.010650930523147408	4.383098980719098E-5	0.0	4.383098980719098E-5	0.0
5	0.010764891096646105	4.383098980719098E-5	0.0	4.383098980719098E-5	0.0
6	0.010975279847720622	4.383098980719098E-5	0.0	5.259718776862918E-5	0.0
7	0.011054175629373567	5.259718776862918E-5	0.0	5.259718776862918E-5	0.0
8	0.011308395370255273	5.259718776862918E-5	0.0	5.259718776862918E-5	0.0
9	0.011571381309098419	5.259718776862918E-5	8.766197961438197E-6	5.259718776862918E-5	0.0
10-11	0.01224637855212916	5.259718776862918E-5	8.766197961438197E-6	5.698028674934828E-5	0.0
12-13	0.013158063140118734	5.259718776862918E-5	8.766197961438197E-6	7.012958369150558E-5	0.0
14-15	0.014709680179293295	5.259718776862918E-5	8.766197961438197E-6	7.012958369150558E-5	0.0
16-17	0.01648921836546525	5.259718776862918E-5	8.766197961438197E-6	7.012958369150558E-5	0.0
18-19	0.018444080510865962	6.136338573006737E-5	8.766197961438197E-6	7.889578165294377E-5	8.766197961438197E-6
20-21	0.020298131379710142	7.451268267222468E-5	8.766197961438197E-6	8.327888063366286E-5	8.766197961438197E-6
22-23	0.022594875245606952	7.889578165294377E-5	8.766197961438197E-6	9.204507859510107E-5	8.766197961438197E-6
24-25	0.024615483875718457	8.766197961438196E-5	8.766197961438197E-6	9.642817757582016E-5	8.766197961438197E-6
26-27	0.02678950097015513	8.766197961438196E-5	8.766197961438197E-6	9.642817757582016E-5	8.766197961438197E-6
28-29	0.02910816033095553	8.766197961438196E-5	8.766197961438197E-6	9.642817757582016E-5	8.766197961438197E-6
30-31	0.031628442244869014	8.766197961438196E-5	8.766197961438197E-6	1.0519437553725836E-4	1.7532395922876394E-5
32-33	0.034131191762859614	9.204507859510107E-5	8.766197961438197E-6	1.0519437553725836E-4	1.7532395922876394E-5
34-35	0.037107315970767885	1.0081127655653926E-4	8.766197961438197E-6	1.0519437553725836E-4	1.7532395922876394E-5
36-37	0.04002207679294609	1.2272677146013474E-4	8.766197961438197E-6	1.0519437553725836E-4	1.7532395922876394E-5
38-39	0.042660702379338986	1.2272677146013474E-4	8.766197961438197E-6	1.1396057349869656E-4	1.7532395922876394E-5
40-41	0.045706956170938756	1.2272677146013474E-4	8.766197961438197E-6	1.1396057349869656E-4	1.7532395922876394E-5
42-43	0.04885402123909507	1.2710987044085384E-4	8.766197961438197E-6	1.2710987044085384E-4	1.7532395922876394E-5
44-45	0.05225530604813309	1.3587606840229205E-4	8.766197961438197E-6	1.4025916738301115E-4	1.7532395922876394E-5
46-47	0.055722337341881895	1.4902536534444936E-4	8.766197961438197E-6	1.4025916738301115E-4	1.7532395922876394E-5
48-49	0.05926388131830293	1.7094086024804485E-4	8.766197961438197E-6	1.4025916738301115E-4	1.7532395922876394E-5
50-51	0.0631604563121622	1.8409015719020213E-4	8.766197961438197E-6	1.4025916738301115E-4	1.7532395922876394E-5
52-53	0.06692992143558063	1.9723945413235942E-4	8.766197961438197E-6	1.4902536534444936E-4	1.7532395922876394E-5
54-55	0.07087909361720854	2.103887510745167E-4	8.766197961438197E-6	1.6217466228660664E-4	1.7532395922876394E-5
56-57	0.07537177007244561	2.191549490359549E-4	8.766197961438197E-6	1.7532395922876393E-4	1.7532395922876394E-5
58-59	0.08046931418702191	2.191549490359549E-4	8.766197961438197E-6	1.9285635515164034E-4	1.7532395922876394E-5
60-61	0.08598325270476655	2.23538048016674E-4	8.766197961438197E-6	2.366873449588313E-4	1.7532395922876394E-5
62-63	0.09179962505218078	2.410704439395504E-4	8.766197961438197E-6	2.542197408817077E-4	2.629859388431459E-5
64-65	0.09809375518849342	2.542197408817077E-4	8.766197961438197E-6	2.6736903782386497E-4	2.629859388431459E-5
66-67	0.10496207129128024	2.542197408817077E-4	8.766197961438197E-6	2.761352357853032E-4	2.629859388431459E-5
68-69	0.1124790860432135	2.542197408817077E-4	8.766197961438197E-6	2.980507306888987E-4	2.629859388431459E-5
70-71	0.12130664739038176	2.586028398624268E-4	8.766197961438197E-6	3.155831266117751E-4	2.629859388431459E-5
72-73	0.1313176454623442	2.717521368045841E-4	8.766197961438197E-6	3.2873242355393236E-4	2.629859388431459E-5
74-75	0.14377441276554787	2.805183347660223E-4	8.766197961438197E-6	3.5064791845752785E-4	2.629859388431459E-5
76-77	0.1581948084121137	3.024338296696178E-4	8.766197961438197E-6	3.5941411641896606E-4	2.629859388431459E-5
78-79	0.17497769440928712	3.199662255924942E-4	8.766197961438197E-6	3.5941411641896606E-4	2.629859388431459E-5
80-81	0.19648994420665644	3.2873242355393236E-4	8.766197961438197E-6	3.637972153996852E-4	2.629859388431459E-5
82-83	0.2263125496714692	3.331155225346515E-4	8.766197961438197E-6	3.6818031438040427E-4	2.629859388431459E-5
84-85	0.26804841816587643	3.5503101743824693E-4	8.766197961438197E-6	3.769465123418425E-4	2.629859388431459E-5
86-87	0.3196462593669017	3.6818031438040427E-4	8.766197961438197E-6	4.207775021490334E-4	2.629859388431459E-5
88	0.3665322691636539	3.6818031438040427E-4	8.766197961438197E-6	4.47076096033348E-4	2.629859388431459E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTAT	1375	0.0	11.360301	1
CGTATCG	1075	0.0	10.562718	3
CTCCGAT	2375	0.0	9.965177	1
GATCGGA	8575	0.0	9.639361	94
CGGGGTT	3600	0.0	8.678007	1
CTCGCGT	1060	8.067218E-9	8.484502	1
CCCGGTT	2780	0.0	8.343147	1
CGTCGAT	1330	1.05501385E-10	8.18568	1
CTCGGAT	3125	0.0	8.179416	1
CGCCGAT	1800	0.0	8.152067	1
CGCCAAT	2270	0.0	8.132373	1
CGCGGAT	1290	4.838512E-10	8.072565	1
TAGATCG	1805	0.0	8.065926	94
AGATCGG	10455	0.0	8.040791	94
CTCGACT	1920	0.0	7.889098	1
TACCGTA	790	5.1184477E-5	7.7964497	9
CCCCGTT	2310	0.0	7.7866416	1
CGCGTAC	610	0.0020608355	7.759768	1
CTCCGTT	2765	0.0	7.70364	1
CGCCATT	2620	0.0	7.5879874	1
>>END_MODULE