FastQCFastQC Report
Wed 25 May 2016
SRR1033290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11407454
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG10300.010.999723
GTATCGA12900.010.24664
CCCGTAT15400.010.1245551
CTCGCGT10053.092282E-119.87271
GCGAACG5355.4110824E-59.7061393
CTCGCGA4409.5271406E-49.6643481
GTCCGAT9451.0550139E-99.4995811
CCGGGAT34100.09.4218721
GATCGGA88900.09.40647594
CCCGGAT38550.09.1922031
CTCGGTT21650.09.1658781
CTCCGAT23000.09.0387321
CCCGGTT29300.09.0303191
TCCGATT18850.09.0195792
CTCGGAT32300.08.7767031
CTCGTTT23300.08.7195731
CCCGTTT25150.08.2660371
CTCGAAT27200.08.1641631
CGCCATT26200.08.11511
CTCAGAT93700.08.0679471
Produced by FastQC (version 0.11.5)