FastQCFastQC Report
Wed 25 May 2016
SRR1033289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12007230
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT14900.010.801661
CGCCAAT22350.010.1662681
CTCGGAT34900.09.901371
CTCGCGT10304.9112714E-119.6511681
CGCGAAT4450.00103856389.5737111
CGACGAT10456.548362E-119.5126351
TCGGATA10153.8016879E-109.3222892
CGTATCG11807.2759576E-129.22322753
CTCGTAT14200.09.0006371
CGAATCG6353.4773147E-58.9422053
CGCGGAT13351.8189894E-128.8645481
CGGGGTT35400.08.8254831
CCGGGAT34550.08.7685891
GTATCGA13451.4551915E-118.4433214
GTCGGAT14053.6379788E-128.4228981
TCCGATT20000.08.2793582
CGCGGAG25750.08.272431
CGCGTTT8107.992863E-68.1816491
CGCTAAT6959.9621626E-58.1732411
CCGCAAT22350.08.0482961
Produced by FastQC (version 0.11.5)