FastQCFastQC Report
Wed 25 May 2016
SRR1033289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12007230
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT13500.010.8441081
CTCGGTT21200.010.6923031
CCCGTAT16750.09.8677721
CGCGTAA5405.967856E-59.6197731
CCGGGAT34550.09.56788351
CCCGGAT36300.09.3668111
CTCGGAT32600.08.8364561
CGTATGC23750.08.70623944-45
CTCGTTT21550.08.54641
CTCCGAT22750.08.5107591
CTCGCAT27300.08.4761631
CGTATCG11202.5520421E-98.426853
CTCGTAT25100.08.14622842-43
CACGATT20000.08.0281371
GTATCGA13601.8007995E-107.98092374
CCCGTCT43200.07.98003861
CGGAAAT23800.07.9368641
CGGGAAT27450.07.91373251
TCCGATT19900.07.82884552
TCAATCG13903.1468517E-107.7741738
Produced by FastQC (version 0.11.5)