FastQCFastQC Report
Wed 25 May 2016
SRR1033288_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033288_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10927215
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAT30100.010.0639071
GCGAACG4801.9037357E-49.8566933
CGCATAC5851.394104E-48.8999981
CGACCGT8057.7758887E-78.81959151
CGCCAGT34150.08.7317831
AGATCGG75900.08.60156294
GTCCGAT9459.8556484E-88.5147171
CCCCGTT23350.08.5136441
CTGGAAT113250.08.48420051
ACGTTCG6152.4152492E-48.4599886
GTCGATT10153.4806362E-88.3938191
CTCGGGT29900.08.3899191
CCCGTAT15800.08.3879151
CTCGTTT21450.08.3851221
CTCCGAT21350.08.2027021
CGCGCAT5850.00136527218.0909071
CTCGTAT12301.6261765E-98.081041
CGATTCA15303.6379788E-128.0395244
CGGGGTT34100.07.9117691
ATACGAC6655.5099174E-47.83591278
Produced by FastQC (version 0.11.5)