FastQCFastQC Report
Wed 25 May 2016
SRR1033287_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033287_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12281605
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAAAT25150.010.3495911
CCGGGAT37450.09.9832931
CGCCAAT21150.09.1742811
CGCGTAT4650.00155495559.1598441
CCGGATT35700.09.014451
CCCGGTT32100.08.9933871
CCCGTAT15850.08.9575771
CTCGGTT22650.08.7756571
GATCGGA87350.08.65672494
CCGTAAT11756.657501E-108.458241
CGAGAAT24900.08.3628031
CCCGGAT39150.08.3409561
CGCTAAT5150.00392554478.2705381
CGACCGT7452.4935467E-58.25820351
CGCCAGT37200.08.0148641
CGCGTAA5350.00553135857.961361
CGTAATA7754.1095827E-57.9349442
GTCGTTA10759.215364E-87.924331
CCGCAAT21350.07.75833751
CTCGAAT27500.07.7442321
Produced by FastQC (version 0.11.5)