##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033287_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12281605
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24484202186929	34.0	31.0	34.0	31.0	34.0
2	32.41050571159062	34.0	31.0	34.0	31.0	34.0
3	32.49418353708656	34.0	31.0	34.0	31.0	34.0
4	35.80101721232689	37.0	37.0	37.0	35.0	37.0
5	35.79943142610433	37.0	37.0	37.0	35.0	37.0
6	35.73169329252976	37.0	37.0	37.0	35.0	37.0
7	35.695365548721036	37.0	37.0	37.0	35.0	37.0
8	35.682517960803985	37.0	37.0	37.0	35.0	37.0
9	37.43865968658005	39.0	39.0	39.0	35.0	39.0
10-11	37.55067696770902	39.0	39.0	39.0	35.0	39.0
12-13	37.48685355049279	39.0	39.0	39.0	35.0	39.0
14-15	38.977253339445454	41.0	40.0	41.0	36.0	41.0
16-17	39.0758284442465	41.0	40.0	41.0	36.0	41.0
18-19	39.12537660997891	41.0	40.0	41.0	36.0	41.0
20-21	39.08904715629594	41.0	39.5	41.0	36.0	41.0
22-23	39.02336535004993	41.0	39.0	41.0	36.0	41.0
24-25	38.97611554027344	41.0	39.0	41.0	35.5	41.0
26-27	38.90266166352036	41.0	39.0	41.0	35.0	41.0
28-29	38.805929965993855	41.0	39.0	41.0	35.0	41.0
30-31	38.804683345539935	41.0	39.0	41.0	35.0	41.0
32-33	38.88666550503782	41.0	39.0	41.0	35.0	41.0
34-35	38.821063777902	41.0	39.0	41.0	35.0	41.0
36-37	38.764726515793335	41.0	39.0	41.0	35.0	41.0
38-39	38.68145873442437	41.0	39.0	41.0	35.0	41.0
40-41	38.548910789754274	41.0	39.0	41.0	34.5	41.0
42-43	38.43366762731744	41.0	38.5	41.0	34.0	41.0
44-45	38.3018828565159	41.0	38.0	41.0	34.0	41.0
46-47	38.1736478660566	40.0	38.0	41.0	34.0	41.0
48-49	38.030232652816956	40.0	38.0	41.0	33.0	41.0
50-51	37.85423053420135	40.0	37.0	41.0	33.0	41.0
52-53	37.666645849626335	40.0	37.0	41.0	33.0	41.0
54-55	37.46175748202292	40.0	36.0	41.0	33.0	41.0
56-57	37.22605555218557	39.5	35.5	41.0	32.0	41.0
58-59	36.97990356309293	39.0	35.0	41.0	32.0	41.0
60-61	36.71280117704486	39.0	35.0	41.0	32.0	41.0
62-63	36.41853613595291	38.5	35.0	41.0	31.0	41.0
64-65	36.139478797763	37.5	35.0	40.5	31.0	41.0
66-67	35.81987431610119	37.0	35.0	40.0	31.0	41.0
68-69	35.49608223029482	36.5	35.0	39.5	31.0	41.0
70-71	35.16798435546494	36.0	35.0	39.0	31.0	41.0
72-73	34.81792049980438	35.0	35.0	39.0	30.0	41.0
74-75	34.48598644069729	35.0	34.5	37.5	30.0	40.0
76-77	34.23023489193798	35.0	34.5	37.0	30.0	39.0
78-79	34.0611990045275	35.0	35.0	37.0	30.0	39.0
80-81	33.80325942741197	35.0	34.0	36.0	30.0	39.0
82-83	33.57317883126839	35.0	34.0	36.0	30.0	37.0
84-85	33.355974728058754	35.0	34.0	35.5	30.0	37.0
86-87	33.162192360037636	35.0	34.0	35.0	30.0	36.5
88-89	32.988717924082394	35.0	34.0	35.0	29.5	36.0
90-91	32.86371435166658	35.0	34.0	35.0	29.0	36.0
92-93	32.716588426349816	35.0	34.0	35.0	29.0	36.0
94-95	32.590398364057464	35.0	34.0	35.0	29.0	36.0
96-97	32.513226365772226	35.0	34.0	35.0	29.0	35.0
98-99	32.423700729668475	35.0	34.0	35.0	29.0	35.0
100	32.33037196685612	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.01509002956521499
1101	2	-0.002516677543766832
1101	3	0.033799413268724265
1101	4	0.016820620334932812
1101	5	0.03978756662295524
1101	6	0.07710065514192621
1101	7	0.17112836369023654
1101	8	0.1436792694018436
1101	9	0.18959019663454058
1101	10-11	0.2141659105370124
1101	12-13	0.15501252544058275
1101	14-15	0.25918553486668117
1101	16-17	0.2880900506332793
1101	18-19	0.2516435319175514
1101	20-21	0.3225495623518313
1101	22-23	0.3245165809100641
1101	24-25	0.29317015188050277
1101	26-27	0.16374167037566423
1101	28-29	0.19961129255834464
1101	30-31	0.28416356933142595
1101	32-33	0.2141050919797749
1101	34-35	0.29182555493719775
1101	36-37	0.23084434785840813
1101	38-39	0.2601163932377517
1101	40-41	0.23725961245192195
1101	42-43	0.22048034674903505
1101	44-45	0.16631467154601864
1101	46-47	0.13315413214856875
1101	48-49	0.1789622718224564
1101	50-51	0.1769475445568176
1101	52-53	0.17209709553746677
1101	54-55	0.21030348747449068
1101	56-57	0.19767087892906687
1101	58-59	0.17021838289697655
1101	60-61	0.16595299838954247
1101	62-63	0.1729540611779825
1101	64-65	0.14885960044176016
1101	66-67	0.09926274756551834
1101	68-69	0.1664924597493922
1101	70-71	0.18860824091602524
1101	72-73	0.16973347206144496
1101	74-75	0.09704618470193793
1101	76-77	0.16896221937625455
1101	78-79	0.20707874914177893
1101	80-81	0.18091280034563795
1101	82-83	0.20881454079086126
1101	84-85	0.26215986261031077
1101	86-87	0.21269159861949305
1101	88-89	0.23526356426771855
1101	90-91	0.21596645449507434
1101	92-93	0.21697821846893106
1101	94-95	0.21498145928656243
1101	96-97	0.18994265184220893
1101	98-99	0.15299418470646486
1101	100	0.19283032820265333
1102	1	0.06568896170323058
1102	2	0.05763511296797219
1102	3	0.0639846116098326
1102	4	0.014907816343267655
1102	5	0.06687341414092884
1102	6	0.1187771070491479
1102	7	0.15683067548065566
1102	8	0.18216074285570016
1102	9	0.22399718893490927
1102	10-11	0.25172351147808314
1102	12-13	0.122124262807084
1102	14-15	0.3109162868774362
1102	16-17	0.25568409917581647
1102	18-19	0.25752015648744475
1102	20-21	0.22983467834357896
1102	22-23	0.2619242495924041
1102	24-25	0.2620586677110168
1102	26-27	0.25993592699627754
1102	28-29	0.2673410448531186
1102	30-31	0.32457732927258576
1102	32-33	0.3101516838527658
1102	34-35	0.3064975081727752
1102	36-37	0.26490445118150063
1102	38-39	0.2553642229002975
1102	40-41	0.278426050331241
1102	42-43	0.30735812423017705
1102	44-45	0.28256297738590064
1102	46-47	0.30251433553878115
1102	48-49	0.3239721209153217
1102	50-51	0.312962438535088
1102	52-53	0.31149392451117563
1102	54-55	0.27547422025112667
1102	56-57	0.3074620572449831
1102	58-59	0.356870646091771
1102	60-61	0.3276798342929865
1102	62-63	0.38071979399709477
1102	64-65	0.28723094626088397
1102	66-67	0.27387578372317023
1102	68-69	0.20620303986068222
1102	70-71	0.23656449192879592
1102	72-73	0.2393544593398076
1102	74-75	0.23453875684336367
1102	76-77	0.2832684849116589
1102	78-79	0.29099679616211915
1102	80-81	0.28070086095947033
1102	82-83	0.2929805057106023
1102	84-85	0.31016842726622684
1102	86-87	0.2686415804416953
1102	88-89	0.2865593915595852
1102	90-91	0.23335547428119696
1102	92-93	0.25084055560720486
1102	94-95	0.33434148419198095
1102	96-97	0.29128104147391554
1102	98-99	0.2584539391747285
1102	100	0.2713522769811334
1103	1	-0.010866863382368308
1103	2	-0.0061121802037007456
1103	3	0.00752519459781098
1103	4	0.0034568722826762155
1103	5	0.030926116248785718
1103	6	0.0738328159537005
1103	7	0.11213051094752302
1103	8	0.13542985500808413
1103	9	0.15433549151249082
1103	10-11	0.1777524807819617
1103	12-13	0.09004090816967647
1103	14-15	0.2589418191651589
1103	16-17	0.20859779182820404
1103	18-19	0.22320732608028493
1103	20-21	0.21833489723173471
1103	22-23	0.22840077054079444
1103	24-25	0.23026586289766726
1103	26-27	0.2144439734736565
1103	28-29	0.19950541037501068
1103	30-31	0.18615708421982902
1103	32-33	0.20413256442416383
1103	34-35	0.22321314028375383
1103	36-37	0.19771423577147829
1103	38-39	0.21977469226695234
1103	40-41	0.25147440485203276
1103	42-43	0.24044216739920898
1103	44-45	0.24375490605823558
1103	46-47	0.21425204402920173
1103	48-49	0.26822319440785236
1103	50-51	0.22030803691252032
1103	52-53	0.24029986143263216
1103	54-55	0.22503202281727397
1103	56-57	0.22450712026664377
1103	58-59	0.2646222490002401
1103	60-61	0.2496848972641672
1103	62-63	0.24504414316919565
1103	64-65	0.18973077049078313
1103	66-67	0.18979728825681264
1103	68-69	0.19169291657054544
1103	70-71	0.2096975271745336
1103	72-73	0.1906431103919175
1103	74-75	0.18664723609485634
1103	76-77	0.22754701434275404
1103	78-79	0.24233390642306318
1103	80-81	0.22663350384776493
1103	82-83	0.26475465266473464
1103	84-85	0.24878765111287038
1103	86-87	0.23773168150339075
1103	88-89	0.2642677721553284
1103	90-91	0.26298523022514075
1103	92-93	0.30818905053204304
1103	94-95	0.3171692390413199
1103	96-97	0.2931015101492349
1103	98-99	0.2881859785319989
1103	100	0.28183973018030173
1104	1	-0.037428414613373207
1104	2	-0.01904025621349348
1104	3	0.0092111152791432
1104	4	-0.02291723792993139
1104	5	-0.018098489369116066
1104	6	0.057135551022639675
1104	7	0.09074558492122975
1104	8	0.07483365333089154
1104	9	0.09030200907771047
1104	10-11	0.12801425933133004
1104	12-13	0.06178696011983931
1104	14-15	0.18109604951588665
1104	16-17	0.17407068232514433
1104	18-19	0.18407274704983934
1104	20-21	0.17552579342501673
1104	22-23	0.1795499651616268
1104	24-25	0.16719633937712075
1104	26-27	0.17215190519385004
1104	28-29	0.14534067029808995
1104	30-31	0.1195429466124267
1104	32-33	0.11254585341639967
1104	34-35	0.15677825153806424
1104	36-37	0.16795787973783405
1104	38-39	0.18866477456387543
1104	40-41	0.1424548543260329
1104	42-43	0.13810517995858618
1104	44-45	0.16117835465283292
1104	46-47	0.1191735835087897
1104	48-49	0.09704274377352817
1104	50-51	0.1350572461282482
1104	52-53	0.1145283011196625
1104	54-55	0.12980023895488557
1104	56-57	0.0679973683499
1104	58-59	0.0986103083027885
1104	60-61	0.08484796486896329
1104	62-63	0.09511483197509563
1104	64-65	0.0784019299822134
1104	66-67	0.061509016328656685
1104	68-69	0.06362767471004815
1104	70-71	0.04415111696431495
1104	72-73	0.07423098423858221
1104	74-75	0.04799731539870322
1104	76-77	0.055119080682167976
1104	78-79	0.09476141999096654
1104	80-81	0.08799540655863325
1104	82-83	0.09904656269332435
1104	84-85	0.1025929708002522
1104	86-87	0.1137751207636839
1104	88-89	0.11735610338575242
1104	90-91	0.1355529862459406
1104	92-93	0.19058293501718282
1104	94-95	0.21355071380706647
1104	96-97	0.09386164023676002
1104	98-99	0.1310173241839081
1104	100	0.1457018064153104
1105	1	-0.0918129660433209
1105	2	-0.10648467620305979
1105	3	-0.047243200714646605
1105	4	-0.044490992578495536
1105	5	0.002017377690982869
1105	6	0.04260965812976991
1105	7	0.0703219742652621
1105	8	0.05469888982144511
1105	9	0.07517195360204454
1105	10-11	0.11785959752921116
1105	12-13	-0.0029891201048073412
1105	14-15	0.12702958334386238
1105	16-17	0.18811842708861093
1105	18-19	0.15259392783128334
1105	20-21	0.1516081513783334
1105	22-23	0.16268036057446267
1105	24-25	0.11498196890586598
1105	26-27	0.13253196351227103
1105	28-29	0.16865606758582885
1105	30-31	0.18503007701984586
1105	32-33	0.12452269154115925
1105	34-35	0.14667759048391105
1105	36-37	0.10715334417891142
1105	38-39	0.1210218228416835
1105	40-41	0.1387260196339284
1105	42-43	0.10618445235378715
1105	44-45	0.16512190936735038
1105	46-47	0.11866532206402525
1105	48-49	0.09838806653930732
1105	50-51	0.1409647850722493
1105	52-53	0.14120567345367618
1105	54-55	0.19441673369204437
1105	56-57	0.16120555348285137
1105	58-59	0.14087055968457207
1105	60-61	0.13875250338833212
1105	62-63	0.10099913657432324
1105	64-65	0.10553917061726281
1105	66-67	0.0767298278038453
1105	68-69	0.10118133772542848
1105	70-71	0.15101373550098884
1105	72-73	0.09119355935652607
1105	74-75	0.13295867398313277
1105	76-77	0.18302979442857747
1105	78-79	0.10000496588383356
1105	80-81	0.16126334723717406
1105	82-83	0.11842428305683228
1105	84-85	0.14876312961363425
1105	86-87	0.14637766375501116
1105	88-89	0.18198340225070098
1105	90-91	0.17298571530065487
1105	92-93	0.20419481825820895
1105	94-95	0.1614526277753825
1105	96-97	0.12164197179784253
1105	98-99	0.1144102940483478
1105	100	0.15786031566712921
1106	1	-0.05642243916664569
1106	2	-0.014194628546640331
1106	3	-0.026866404196141502
1106	4	-0.06103346148452715
1106	5	-0.033261855522908945
1106	6	0.03982085604563679
1106	7	0.04845074867981225
1106	8	0.07408919120573643
1106	9	0.05769876801920759
1106	10-11	0.1164727719511589
1106	12-13	0.07909123839811372
1106	14-15	0.15209993264572574
1106	16-17	0.1451179347020357
1106	18-19	0.11330739958801672
1106	20-21	0.10980790237784532
1106	22-23	0.14246012467786073
1106	24-25	0.09228060594919185
1106	26-27	0.13136556659323873
1106	28-29	0.11373563368158557
1106	30-31	0.1387770842469962
1106	32-33	0.14318209537159277
1106	34-35	0.16775255303894454
1106	36-37	0.15676841521705853
1106	38-39	0.22288997159947144
1106	40-41	0.20578757785446555
1106	42-43	0.18505448189549156
1106	44-45	0.16760201439016242
1106	46-47	0.18799675688985218
1106	48-49	0.19197194677200002
1106	50-51	0.1775695185318753
1106	52-53	0.15737154176265022
1106	54-55	0.2099051074422178
1106	56-57	0.17770969754040067
1106	58-59	0.18158216712147635
1106	60-61	0.1764139784608787
1106	62-63	0.1660410082637256
1106	64-65	0.17579599513785382
1106	66-67	0.1584843840583119
1106	68-69	0.1738630927425291
1106	70-71	0.16281566733227493
1106	72-73	0.17523204213144083
1106	74-75	0.2022335421440431
1106	76-77	0.1822874500574727
1106	78-79	0.19405637396128128
1106	80-81	0.13347130188815015
1106	82-83	0.17100624926771246
1106	84-85	0.1919234082336274
1106	86-87	0.1747558056585845
1106	88-89	0.19217320039128083
1106	90-91	0.17194459692084507
1106	92-93	0.15296095480179162
1106	94-95	0.1366707577797115
1106	96-97	0.17594419704672504
1106	98-99	0.1583583016876844
1106	100	0.1695473643168981
1107	1	-0.11836721918929527
1107	2	-0.11687986728932742
1107	3	-0.06612643423978426
1107	4	-0.015936726870428686
1107	5	-0.020392882231725196
1107	6	0.04477555743279282
1107	7	0.0565079558827577
1107	8	0.0809578884589186
1107	9	0.0458619453406115
1107	10-11	0.09907552106082562
1107	12-13	0.024779686778629184
1107	14-15	0.14914323849957611
1107	16-17	-0.029730172451245096
1107	18-19	0.024773031815648494
1107	20-21	0.015309934853284801
1107	22-23	-0.004023545304221443
1107	24-25	0.012750781807255862
1107	26-27	0.04181459845042923
1107	28-29	0.02473755458834148
1107	30-31	-0.00463700829550362
1107	32-33	-0.02507427232534809
1107	34-35	-0.0029024928833223385
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2216	20-21	-0.34597191905293556
2216	22-23	-0.35635306086719254
2216	24-25	-0.2890502963861792
2216	26-27	-0.343141879576919
2216	28-29	-0.33061049512778595
2216	30-31	-0.34612004831825516
2216	32-33	-0.32750815285606194
2216	34-35	-0.37392502956698337
2216	36-37	-0.3891305337820228
2216	38-39	-0.272591215528621
2216	40-41	-0.6107301865172161
2216	42-43	-0.8100680504767439
2216	44-45	-0.5104799500319714
2216	46-47	-0.41671895333498554
2216	48-49	-0.47189778777224944
2216	50-51	-0.4477167546869367
2216	52-53	-0.4163691983506652
2216	54-55	-0.523479105395154
2216	56-57	-0.40545878468282837
2216	58-59	-0.5099510151137849
2216	60-61	-0.5624513668259112
2216	62-63	-0.5218216690082116
2216	64-65	-0.5509275094239214
2216	66-67	-0.4953332490332656
2216	68-69	-0.5515943358639532
2216	70-71	-0.5447913763933983
2216	72-73	-0.5860938418330335
2216	74-75	-0.47476657684737233
2216	76-77	-0.5062913906594204
2216	78-79	-0.5453335539795532
2216	80-81	-0.49527470690178177
2216	82-83	-0.6632531032164053
2216	84-85	-0.5138092059363757
2216	86-87	-0.42640293146775576
2216	88-89	-0.47308098633182993
2216	90-91	-0.6346579621851305
2216	92-93	-0.9665205022802326
2216	94-95	-0.8262279052751325
2216	96-97	-0.8311917928235353
2216	98-99	-0.6570489287682548
2216	100	-0.5986384487631327
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59710.0
3	10863.0
4	8333.0
5	7768.0
6	9619.0
7	12491.0
8	13241.0
9	13406.0
10	15768.0
11	15218.0
12	15723.0
13	16915.0
14	17985.0
15	18209.0
16	19210.0
17	19862.0
18	19712.0
19	18764.0
20	19386.0
21	19969.0
22	22001.0
23	26241.0
24	35634.0
25	53458.0
26	72157.0
27	78553.0
28	87122.0
29	97049.0
30	119022.0
31	151666.0
32	196469.0
33	263809.0
34	380019.0
35	624037.0
36	1318376.0
37	2914542.0
38	3980993.0
39	1503144.0
40	5161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.31217450499838	12.552195609502403	12.437401339067007	47.698228546432205
2	21.515643538295127	19.942908598756976	34.80312412423948	23.73832373870842
3	25.81360910425085	22.574027036060627	23.65772339385614	27.95464046583238
4	27.489321900261903	29.334289680816983	18.641885646645427	24.534502772275687
5	28.416446415954198	31.019113748727012	21.034527547387423	19.529912287931364
6	22.868697760873317	33.667110419710774	22.012655163205547	21.451536656210365
7	20.213206857193516	17.950434782251808	39.0008073032419	22.835551057312784
8	21.22899779152564	21.77171695885089	28.751049479073203	28.248235770550263
9	21.602941116063175	20.962673293762453	30.89403355994167	26.5403520302327
10-11	25.22443883649887	29.94860488163716	21.37737364242876	23.44958263943521
12-13	23.5237701056039	24.146372528507918	27.08852702965723	25.241330336230956
14-15	23.896527894975115	25.309857270985653	26.468048918954473	24.325565915084756
16-17	24.686381723666994	25.403964090053744	25.42347080394702	24.486183382332243
18-19	24.468472947456277	25.5842503542808	25.314411107731612	24.63286559053131
20-21	24.341566876183787	25.66068911955246	25.592691616296335	24.405052387967416
22-23	24.554144185552296	25.73375385383262	25.527192903533376	24.184909057081708
24-25	24.364307433759674	25.867258391716717	25.52389121780093	24.244542956722675
26-27	24.206552241130293	25.95015639106622	25.568490940717965	24.274800427085527
28-29	24.35732238574516	25.620712028607134	25.515338289562035	24.50662729608567
30-31	24.195928678696806	25.719281813551348	25.58687721502996	24.49791229272189
32-33	24.14295607129524	25.893126346271515	25.708158664930192	24.255758917503044
34-35	24.484559206085294	25.779999613101996	25.35626077332766	24.37918040748505
36-37	24.3039002981214	25.690078486682573	25.437386934280575	24.568634280915457
38-39	24.224888869961028	25.802021579578856	25.580370187952973	24.392719362507144
40-41	24.470963734523245	25.69350622249358	25.44381170221698	24.391718340766197
42-43	24.240527258727727	25.608825352983356	25.687192068543474	24.46345531974545
44-45	24.25888293438521	25.737152979501644	25.725590722164416	24.278373363948734
46-47	24.37305244421115	25.734061215031662	25.459242253794063	24.43364408696312
48-49	24.271492929951485	25.614828453508736	25.628817283570676	24.484861332969107
50-51	24.25942484264357	25.745525907376727	25.71521020354876	24.279839046430947
52-53	24.32839323392295	25.782307797920506	25.49437592965522	24.39492303850132
54-55	24.1534342255593	25.669299907841314	25.616939426757483	24.5603264398419
56-57	24.180211867429723	25.72851710441028	25.721757796519405	24.369513231640585
58-59	24.345058805033215	25.683063516347254	25.575562163207593	24.39631551541194
60-61	24.17562474757118	25.754914015630735	25.65425636772844	24.415204869069644
62-63	24.08263847012841	25.902016021061858	25.783998110343404	24.23134739846633
64-65	24.246947050157065	25.793619902179	25.573018131148515	24.386414916515424
66-67	24.216480421890456	25.7178558682179	25.643909331360337	24.421754378531304
68-69	24.225453669573128	25.859762540280713	25.726939167865098	24.187844622281066
70-71	24.310075719857906	25.759799953775964	25.564852627795716	24.365271698570414
72-73	24.181902478851296	25.814418600103316	25.618861335232413	24.384817585812975
74-75	24.15097228314801	25.94272337814009	25.659597226928167	24.246707111783728
76-77	24.381272693606608	25.74331621843895	25.56466449763016	24.310746590324282
78-79	24.22539684881947	25.63350347744486	25.749336100445998	24.391763573289673
80-81	24.172505267146068	25.851810597133774	25.781494997317246	24.194189138402912
82-83	24.265563989051252	25.738397277908632	25.64996566086893	24.346073072171187
84-85	24.22611699525755	25.730269944123723	25.744350778883902	24.299262281734823
86-87	24.23303494292294	25.887710791084018	25.783097391986775	24.09615687400626
88-89	24.31685636284236	25.820510254964457	25.56042478520991	24.30220859698327
90-91	24.220287415952217	25.797979234937408	25.615752788119238	24.365980560991137
92-93	24.188113626631967	25.951900640503943	25.741278692157728	24.118707040706365
94-95	24.480103715110207	25.909997857476185	25.46644325067496	24.143455176738648
96-97	24.360370545057023	25.82985506377524	25.60260064245265	24.207173748715086
98-99	24.386480732825273	25.944252479958624	25.625340345487125	24.043926441728978
100	24.540417338573526	25.758364404437962	25.322376643446393	24.378841613542118
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	66.0
1	45.5
2	30.5
3	52.0
4	89.5
5	130.0
6	184.5
7	256.0
8	358.5
9	497.5
10	603.5
11	725.0
12	872.5
13	976.5
14	1093.5
15	1268.0
16	1497.0
17	1909.5
18	2539.0
19	3466.0
20	5149.5
21	7855.0
22	11960.0
23	17815.0
24	25450.5
25	35652.5
26	48700.0
27	63695.0
28	79583.0
29	95540.0
30	112234.0
31	129676.0
32	148027.0
33	166899.5
34	185093.5
35	205439.5
36	228873.0
37	253547.5
38	274629.5
39	293698.5
40	310128.5
41	324714.0
42	342040.0
43	360983.5
44	373493.0
45	384189.0
46	398448.5
47	403849.0
48	406971.0
49	410174.5
50	413909.0
51	415683.5
52	408136.0
53	402072.5
54	396818.0
55	389627.5
56	374396.5
57	353847.5
58	340300.0
59	328926.0
60	317180.5
61	301201.0
62	279672.0
63	259899.5
64	238387.5
65	211371.5
66	179523.5
67	150545.0
68	125328.0
69	96444.5
70	68133.5
71	44881.5
72	28198.0
73	16421.5
74	8850.0
75	4404.5
76	2269.5
77	1305.0
78	798.5
79	487.5
80	319.5
81	231.0
82	165.0
83	117.5
84	93.5
85	79.0
86	76.0
87	57.0
88	40.0
89	34.5
90	31.0
91	28.0
92	23.0
93	19.0
94	16.0
95	15.0
96	16.0
97	14.5
98	10.5
99	13.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.6779325666311529
2	0.5182791662816058
3	0.5126447235520113
4	0.500748884205281
5	0.5156655013738025
6	0.7217053471431462
7	0.8066535277758892
8	0.857591495574072
9	0.8903966541832277
10-11	0.3825232939831561
12-13	0.6245559924781818
14-15	0.44361872898534027
16-17	0.030785878555775078
18-19	2.727656523719823E-4
20-21	0.026161075852871023
22-23	0.0
24-25	0.0
26-27	0.005638513858734261
28-29	0.008394668286433248
30-31	0.002520028937585926
32-33	0.0
34-35	0.036375538864830775
36-37	2.524100066725807E-4
38-39	0.008626722647406427
40-41	0.11411374979084575
42-43	0.09548833397589322
44-45	0.07258823256406634
46-47	0.04200998159442516
48-49	0.1776925735683569
50-51	0.08742342714979028
52-53	0.07254752127266755
54-55	0.11985404187807702
56-57	0.07847915642947319
58-59	0.09764196129088992
60-61	0.12841155533010545
62-63	0.15518737168309843
64-65	0.060672037571636604
66-67	0.07511233263079214
68-69	0.07565786393553611
70-71	0.03617198240783676
72-73	0.07315819064364959
74-75	0.1766788624125267
76-77	0.15859897790231814
78-79	0.12726756804179912
80-81	0.1549838152261044
82-83	0.10424533275577581
84-85	0.1938590273828217
86-87	0.19227942927654815
88-89	0.11010368758806362
90-91	0.08638121808998091
92-93	0.17881620521096386
94-95	0.12780902821740317
96-97	0.17552673286594056
98-99	0.1941114373894943
100	0.1667615918277782
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2281605E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.45471532358977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.41934084896832	48.61728357535113
2	10.954263982645816	13.244738213006112
3	3.4764091393997445	6.304959746122117
4	1.5804495166114965	3.821825024402123
5	0.900554082719154	2.722137035214149
6	0.5547251134462089	2.012144928974196
7	0.37018594453291803	1.5665640125472224
8	0.2568202758843576	1.2420797334331701
9	0.2050953887070746	1.1159085004620466
>10	1.1711511361082476	12.995533535231244
>50	0.08045539654892082	3.331027761677585
>100	0.03030473499826605	2.924027735670848
>500	2.3095752391895697E-4	0.0837169563534621
>1k	1.3481905606358213E-5	0.01805324155475947
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.01967169600390177	1.6284516559521332E-5	0.0	0.0	0.0
2	0.019687980520461293	4.8853549678564E-5	0.0	8.142258279760666E-6	0.0
3	0.019720549553580335	5.699580795832467E-5	0.0	8.142258279760666E-6	0.0
4	0.020005528593371957	5.699580795832467E-5	0.0	8.142258279760666E-6	0.0
5	0.02013580472584813	5.699580795832467E-5	0.0	1.6284516559521332E-5	0.0
6	0.020314934408002864	5.699580795832467E-5	0.0	1.6284516559521332E-5	0.0
7	0.020477779573598077	7.3280324517846E-5	0.0	1.6284516559521332E-5	0.0
8	0.020681336030592093	8.142258279760666E-5	0.0	1.6284516559521332E-5	0.0
9	0.020941888295544435	8.142258279760666E-5	0.0	3.2569033119042664E-5	0.0
10-11	0.02165840702416337	8.142258279760666E-5	0.0	3.2569033119042664E-5	0.0
12-13	0.022541842047517406	1.0177822849700833E-4	0.0	3.2569033119042664E-5	0.0
14-15	0.0237021138523833	1.3841839075593132E-4	0.0	3.2569033119042664E-5	0.0
16-17	0.025127009051341416	1.5877403645533298E-4	0.0	3.6640162258923E-5	0.0
18-19	0.026922377002028645	1.7098742387497401E-4	0.0	4.8853549678564E-5	0.0
20-21	0.028774740760674197	1.7912968215473468E-4	0.0	5.2924678818444337E-5	0.0
22-23	0.030724811618676875	1.7912968215473468E-4	0.0	5.699580795832467E-5	0.0
24-25	0.032740020542917636	1.9948532785413631E-4	0.0	5.699580795832467E-5	0.0
26-27	0.034673806884360794	2.0355645699401666E-4	0.0	5.699580795832467E-5	0.0
28-29	0.036729727100000366	2.07627586133897E-4	0.0	6.920919537796566E-5	8.142258279760666E-6
30-31	0.03896884812693455	2.1169871527377732E-4	0.0	7.3280324517846E-5	8.142258279760666E-6
32-33	0.041407454481722866	2.2798323183329867E-4	0.0	7.3280324517846E-5	2.0355645699401666E-5
34-35	0.04368728680005586	2.4833887753270033E-4	0.0	7.735145365772633E-5	2.4426774839282E-5
36-37	0.04604447057204657	2.605522649523413E-4	0.0	8.142258279760666E-5	2.4426774839282E-5
38-39	0.04868256225468902	2.727656523719823E-4	0.0	8.956484107736734E-5	2.4426774839282E-5
40-41	0.05134100958303088	2.7683678151186263E-4	0.0	9.7707099357128E-5	2.4426774839282E-5
42-43	0.05413787530212867	2.7683678151186263E-4	0.0	1.1806274505652966E-4	2.4426774839282E-5
44-45	0.05703651924972347	2.849790397916233E-4	0.0	1.3027613247617066E-4	2.4426774839282E-5
46-47	0.0599066652933391	2.849790397916233E-4	0.0	1.3434726161605097E-4	2.4426774839282E-5
48-49	0.0632205644132017	3.1754807291066597E-4	0.0	1.4248951989581166E-4	2.4426774839282E-5
50-51	0.06683979821855532	3.2569033119042665E-4	0.0	1.46560649035692E-4	2.4426774839282E-5
52-53	0.07051602783186725	3.4197484774994803E-4	0.0	1.46560649035692E-4	2.4426774839282E-5
54-55	0.07421668422001848	3.582593643094693E-4	0.0	1.46560649035692E-4	2.4426774839282E-5
56-57	0.07819824851882144	3.78615010008871E-4	0.0	1.46560649035692E-4	2.4426774839282E-5
58-59	0.08244443621171663	3.989706557082727E-4	0.0	1.46560649035692E-4	2.4426774839282E-5
60-61	0.08724836859677543	4.071129139880333E-4	0.0	1.6284516559521333E-4	2.4426774839282E-5
62-63	0.09250012518722106	4.15255172267794E-4	0.0	1.70987423874974E-4	2.4426774839282E-5
64-65	0.09837883566520825	4.15255172267794E-4	0.0	1.70987423874974E-4	2.4426774839282E-5
66-67	0.10429418630545438	4.2339743054755464E-4	0.0	1.7505855301485433E-4	3.2569033119042664E-5
68-69	0.11128024390948903	4.3968194710707596E-4	0.0	1.83200811294615E-4	3.2569033119042664E-5
70-71	0.11951613815946693	4.6410872194635797E-4	0.0	1.95414198714256E-4	3.2569033119042664E-5
72-73	0.1293357016448583	4.681798510862383E-4	0.0	2.1576984441365766E-4	3.2569033119042664E-5
74-75	0.1406005159749072	4.966777550654007E-4	0.0	2.2798323183329867E-4	3.2569033119042664E-5
76-77	0.15318437614627728	5.048200133451614E-4	0.0	2.2798323183329867E-4	3.6640162258923E-5
78-79	0.1682964075135131	5.048200133451614E-4	0.0	2.2798323183329867E-4	4.071129139880333E-5
80-81	0.18662463090125436	5.048200133451614E-4	0.0	2.68694523232102E-4	4.071129139880333E-5
82-83	0.21308697031047652	5.048200133451614E-4	0.0	2.68694523232102E-4	4.071129139880333E-5
84-85	0.25019124129134584	5.088911424850416E-4	0.0	2.7683678151186263E-4	4.071129139880333E-5
86-87	0.29519757393272295	5.129622716249219E-4	0.0	3.379037186100677E-4	4.071129139880333E-5
88	0.33600657243088344	5.129622716249219E-4	0.0	3.41974847749948E-4	4.071129139880333E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAAAT	2515	0.0	10.349591	1
CCGGGAT	3745	0.0	9.983293	1
CGCCAAT	2115	0.0	9.174281	1
CGCGTAT	465	0.0015549555	9.159844	1
CCGGATT	3570	0.0	9.01445	1
CCCGGTT	3210	0.0	8.993387	1
CCCGTAT	1585	0.0	8.957577	1
CTCGGTT	2265	0.0	8.775657	1
GATCGGA	8735	0.0	8.656724	94
CCGTAAT	1175	6.657501E-10	8.45824	1
CGAGAAT	2490	0.0	8.362803	1
CCCGGAT	3915	0.0	8.340956	1
CGCTAAT	515	0.0039255447	8.270538	1
CGACCGT	745	2.4935467E-5	8.2582035	1
CGCCAGT	3720	0.0	8.014864	1
CGCGTAA	535	0.0055313585	7.96136	1
CGTAATA	775	4.1095827E-5	7.934944	2
GTCGTTA	1075	9.215364E-8	7.92433	1
CCGCAAT	2135	0.0	7.7583375	1
CTCGAAT	2750	0.0	7.744232	1
>>END_MODULE