FastQCFastQC Report
Wed 25 May 2016
SRR1033287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033287_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12281605
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT15650.011.7724761
CGCGTAT4154.420926E-511.3833331
CGTATGC29950.010.98397744-45
TATGCCG31000.010.91351546-47
CTCGTAT30000.010.8893442-43
CGACCGT8006.002665E-1010.6291871
CGCGTAC5751.0970223E-59.8589561
CTCGGAT30450.09.7739651
CCCGGTT30400.09.6346431
CGTATCG11555.456968E-129.3994923
CCCGGAT38200.09.1513651
GTATCGA13950.08.7976844
GATCGGA92050.08.77859894
CCGGGAT38200.08.6566961
CGCGAGT7151.5214871E-58.5892421
CTCCGAT24200.08.3940321
CGCTGAT28750.08.3801131
CGGCGAA11353.2105163E-98.3243751
ATCTCGT42150.08.14125940-41
CGCGTGT13351.1823431E-108.1388691
Produced by FastQC (version 0.11.5)