FastQCFastQC Report
Wed 25 May 2016
SRR1033286_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033286_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11386386
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG3354.8356305E-614.1176546
GCGAACG5956.9485395E-1012.7204573
CGATCGT2850.00413732511.6182664
CGCGAGT8106.184564E-1111.1005741
TACCGTA8601.6370905E-1111.014019
CGTATCG10400.010.9163543
TAGATCG14300.010.18134194
CCCGGTT29000.010.1174371
CTCGCGT9358.458301E-109.6165391
GTCCGAT9451.02591E-99.5147771
GTCGATT10751.1459633E-109.244591
AGATCGG87900.09.19007294
CGCGGAT11859.094947E-129.1851511
CCGGGAT34050.09.1728361
CGGCGAA11504.5474735E-119.053191
CGCGTCT10851.3333192E-98.7232251
CTCGGAT28700.08.4094021
CCCGTAT15800.08.3864421
CGGGGTT34150.08.3145241
CGCGATC8602.0313018E-68.2489412
Produced by FastQC (version 0.11.5)