##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033286_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11386386
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21291654788447	34.0	31.0	34.0	31.0	34.0
2	32.36276620167277	34.0	31.0	34.0	31.0	34.0
3	32.45147389171595	34.0	31.0	34.0	31.0	34.0
4	35.78249832738852	37.0	37.0	37.0	35.0	37.0
5	35.78895682967361	37.0	37.0	37.0	35.0	37.0
6	35.717090831103036	37.0	37.0	37.0	35.0	37.0
7	35.67452930192249	37.0	37.0	37.0	35.0	37.0
8	35.667657850348654	37.0	37.0	37.0	35.0	37.0
9	37.42273536133414	39.0	39.0	39.0	35.0	39.0
10-11	37.537706608576244	39.0	39.0	39.0	35.0	39.0
12-13	37.473918414499565	39.0	39.0	39.0	35.0	39.0
14-15	38.95782458982157	41.0	40.0	41.0	36.0	41.0
16-17	39.05876605623593	41.0	39.5	41.0	36.0	41.0
18-19	39.111334404085724	41.0	39.5	41.0	36.0	41.0
20-21	39.07239369014892	41.0	39.5	41.0	36.0	41.0
22-23	39.003821713052766	41.0	39.0	41.0	36.0	41.0
24-25	38.954739941189416	41.0	39.0	41.0	35.5	41.0
26-27	38.88202459498562	41.0	39.0	41.0	35.0	41.0
28-29	38.78198455594251	41.0	39.0	41.0	35.0	41.0
30-31	38.781024066811014	41.0	39.0	41.0	35.0	41.0
32-33	38.86830132054192	41.0	39.0	41.0	35.0	41.0
34-35	38.79588795777694	41.0	39.0	41.0	35.0	41.0
36-37	38.735424172340544	41.0	39.0	41.0	35.0	41.0
38-39	38.65170911121404	41.0	39.0	41.0	35.0	41.0
40-41	38.51540181406111	41.0	39.0	41.0	34.5	41.0
42-43	38.38468641410892	41.0	38.0	41.0	34.0	41.0
44-45	38.24500723934706	40.0	38.0	41.0	34.0	41.0
46-47	38.11563208027552	40.0	38.0	41.0	33.0	41.0
48-49	37.9706266325417	40.0	37.5	41.0	33.0	41.0
50-51	37.79105420279973	40.0	37.0	41.0	33.0	41.0
52-53	37.60371780826682	40.0	37.0	41.0	33.0	41.0
54-55	37.39736119959397	40.0	36.0	41.0	33.0	41.0
56-57	37.15801550202145	39.5	35.5	41.0	32.0	41.0
58-59	36.91179852852345	39.0	35.0	41.0	32.0	41.0
60-61	36.64390549380637	39.0	35.0	41.0	32.0	41.0
62-63	36.35031989078888	38.5	35.0	41.0	31.0	41.0
64-65	36.07417999003371	37.0	35.0	40.5	31.0	41.0
66-67	35.757058648810954	37.0	35.0	40.0	31.0	41.0
68-69	35.43600792209222	36.0	35.0	39.5	31.0	41.0
70-71	35.110981877832	36.0	35.0	39.0	30.5	41.0
72-73	34.760387975605255	35.0	35.0	39.0	30.0	41.0
74-75	34.431437376178884	35.0	34.0	37.5	30.0	40.0
76-77	34.180641864767274	35.0	34.0	37.0	30.0	39.0
78-79	34.01908441361465	35.0	34.0	36.5	30.0	39.0
80-81	33.765009547366475	35.0	34.0	36.0	30.0	38.5
82-83	33.535785893785786	35.0	34.0	36.0	30.0	37.0
84-85	33.320393757949184	35.0	34.0	35.5	30.0	37.0
86-87	33.129502635867084	35.0	34.0	35.0	30.0	36.5
88-89	32.95511130572949	35.0	34.0	35.0	29.0	36.0
90-91	32.829643224812514	35.0	34.0	35.0	29.0	36.0
92-93	32.682604954724	35.0	34.0	35.0	29.0	36.0
94-95	32.55907216741115	35.0	34.0	35.0	29.0	35.5
96-97	32.47996835870486	35.0	34.0	35.0	29.0	35.0
98-99	32.38902220599232	35.0	34.0	35.0	29.0	35.0
100	32.29533268940646	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.01684031791830165
1101	2	-0.05550688374893298
1101	3	-0.015399213742639972
1101	4	0.016596886139716105
1101	5	0.0061055336395057225
1101	6	0.05347612260923995
1101	7	0.1301270696074468
1101	8	0.12617863328308943
1101	9	0.17844536132488287
1101	10-11	0.18956547500091148
1101	12-13	0.10931550737954154
1101	14-15	0.1920799387293286
1101	16-17	0.21897405077704235
1101	18-19	0.18391506047519357
1101	20-21	0.23986369250632578
1101	22-23	0.2768165146139552
1101	24-25	0.2132180405459536
1101	26-27	0.11799105512655927
1101	28-29	0.11880647635452846
1101	30-31	0.23488043820299964
1101	32-33	0.17719447219093354
1101	34-35	0.19871176601508722
1101	36-37	0.1622457218726865
1101	38-39	0.18304464120423347
1101	40-41	0.14950534650464675
1101	42-43	0.10188637767155484
1101	44-45	0.05169077923234511
1101	46-47	0.02638885837770033
1101	48-49	0.07044675398852007
1101	50-51	0.09535373410469106
1101	52-53	0.08030234367691946
1101	54-55	0.11437700706049725
1101	56-57	0.0887216555566539
1101	58-59	0.10700259060374151
1101	60-61	0.09250863426106548
1101	62-63	0.1339779152920073
1101	64-65	0.07767457333306993
1101	66-67	-0.0023317580363553247
1101	68-69	0.06597021914585355
1101	70-71	0.08439152042672049
1101	72-73	0.04612030823205515
1101	74-75	0.017202504166526467
1101	76-77	0.05328905808529072
1101	78-79	0.07680724247504145
1101	80-81	0.08182817059102376
1101	82-83	0.1300131455289133
1101	84-85	0.17383278400525626
1101	86-87	0.09610580157414716
1101	88-89	0.15218970061288672
1101	90-91	0.10493370726435813
1101	92-93	0.10118935629157733
1101	94-95	0.13035147187608942
1101	96-97	0.07886023963841637
1101	98-99	0.02784688974337257
1101	100	0.052463898868147396
1102	1	0.10772057432306781
1102	2	0.08373374949577084
1102	3	0.09834001762956035
1102	4	0.06996157238113909
1102	5	0.09400152027486541
1102	6	0.13431835469559417
1102	7	0.17853593226386977
1102	8	0.19106438697743044
1102	9	0.2305397808166063
1102	10-11	0.27737491571190986
1102	12-13	0.17465272576755098
1102	14-15	0.3621967600244105
1102	16-17	0.3204788966900125
1102	18-19	0.30690517126318184
1102	20-21	0.2842574920927845
1102	22-23	0.3258408595763669
1102	24-25	0.3515595031496943
1102	26-27	0.34869999565986376
1102	28-29	0.3405325861733459
1102	30-31	0.37194052928540344
1102	32-33	0.3866584955375032
1102	34-35	0.39494301679717836
1102	36-37	0.3828608942738896
1102	38-39	0.39567185040176156
1102	40-41	0.36969387186515235
1102	42-43	0.35759621118914
1102	44-45	0.35860892019844925
1102	46-47	0.3737749214804893
1102	48-49	0.389364514441354
1102	50-51	0.401800326127443
1102	52-53	0.425190541594219
1102	54-55	0.39443172177291075
1102	56-57	0.4116029440949731
1102	58-59	0.42129368174678206
1102	60-61	0.4475376184821158
1102	62-63	0.4831431153256318
1102	64-65	0.40635593237517753
1102	66-67	0.39216509617723005
1102	68-69	0.3269735813331849
1102	70-71	0.34591129736168114
1102	72-73	0.3949516521733045
1102	74-75	0.34130289447871576
1102	76-77	0.3963583476547967
1102	78-79	0.4093068696236486
1102	80-81	0.40704241326698565
1102	82-83	0.41382940873003093
1102	84-85	0.4336094366800012
1102	86-87	0.4074600754474389
1102	88-89	0.4363682383751808
1102	90-91	0.35209732478003275
1102	92-93	0.3725858371411661
1102	94-95	0.4686458585507509
1102	96-97	0.4642134898692518
1102	98-99	0.45042041050612625
1102	100	0.48645966318967737
1103	1	0.05070943360093594
1103	2	0.051412816567903974
1103	3	0.05517959341266021
1103	4	0.054314840729439595
1103	5	0.06311341273583082
1103	6	0.1560532316485137
1103	7	0.15327200747857006
1103	8	0.20647222199382043
1103	9	0.24593706900031975
1103	10-11	0.24150545977519755
1103	12-13	0.1730285966990195
1103	14-15	0.3282787922568744
1103	16-17	0.26907364276036105
1103	18-19	0.2957589411468504
1103	20-21	0.298977569557799
1103	22-23	0.3127263358747001
1103	24-25	0.29448848260378924
1103	26-27	0.2987424000685266
1103	28-29	0.2974954251312951
1103	30-31	0.2783296700174205
1103	32-33	0.2978030060893673
1103	34-35	0.3259413406568257
1103	36-37	0.282356383355598
1103	38-39	0.3009776697823767
1103	40-41	0.30125801858429924
1103	42-43	0.2759827664502055
1103	44-45	0.25557656847798427
1103	46-47	0.25340567938475544
1103	48-49	0.30337924762879
1103	50-51	0.2701130595304875
1103	52-53	0.2496441838889325
1103	54-55	0.2770088288648225
1103	56-57	0.2634105688604862
1103	58-59	0.28461708732476865
1103	60-61	0.2453891143697149
1103	62-63	0.27973264621965654
1103	64-65	0.23012668751193388
1103	66-67	0.24815470642540305
1103	68-69	0.2364160386542764
1103	70-71	0.256889312533751
1103	72-73	0.2183412840985639
1103	74-75	0.2418804212091814
1103	76-77	0.2779253705502285
1103	78-79	0.253667210789672
1103	80-81	0.27038941381131565
1103	82-83	0.2890626598458397
1103	84-85	0.28785121754353327
1103	86-87	0.2889862864078552
1103	88-89	0.3354284541708026
1103	90-91	0.3122247039917383
1103	92-93	0.39660606090819783
1103	94-95	0.35820672170353873
1103	96-97	0.3379346992017531
1103	98-99	0.31582214345692705
1103	100	0.33768980728449094
1104	1	-0.006852924199684196
1104	2	-0.009411333629948615
1104	3	0.021453806868258596
1104	4	0.00989725618030235
1104	5	0.002808802766814722
1104	6	0.05816513984355254
1104	7	0.09394842412212512
1104	8	0.07919050254002968
1104	9	0.07791950121949753
1104	10-11	0.13500792580493481
1104	12-13	0.08225514696767533
1104	14-15	0.17858899825054664
1104	16-17	0.16281699056241195
1104	18-19	0.16857171830566386
1104	20-21	0.16784142046833495
1104	22-23	0.182192116705707
1104	24-25	0.14200923285776668
1104	26-27	0.148432318519518
1104	28-29	0.13967063019619275
1104	30-31	0.12392266253318951
1104	32-33	0.1254613744167301
1104	34-35	0.17426982425395465
1104	36-37	0.16670423179591154
1104	38-39	0.18008024859640415
1104	40-41	0.15078333659207743
1104	42-43	0.1882628900027825
1104	44-45	0.18409685766852135
1104	46-47	0.131885713571414
1104	48-49	0.07627962895490725
1104	50-51	0.13820565036651544
1104	52-53	0.15355480557985146
1104	54-55	0.1380413787427699
1104	56-57	0.1070560205956923
1104	58-59	0.13571188442651305
1104	60-61	0.11662431336398527
1104	62-63	0.08992911743081322
1104	64-65	0.07025404166550686
1104	66-67	0.045541784803397434
1104	68-69	0.06849899247880842
1104	70-71	0.09463198553660845
1104	72-73	0.11169615508986652
1104	74-75	0.1410127437633264
1104	76-77	0.11720783486637032
1104	78-79	0.14319009082063872
1104	80-81	0.15070765437580746
1104	82-83	0.13290870134141386
1104	84-85	0.14843175265984598
1104	86-87	0.1465377040579412
1104	88-89	0.16550656979804756
1104	90-91	0.19488614008355398
1104	92-93	0.24681632481824778
1104	94-95	0.27674050305503073
1104	96-97	0.15472299643477783
1104	98-99	0.13441298716298888
1104	100	0.1434351102343001
1105	1	-0.07229395264443639
1105	2	-0.10774682573546812
1105	3	-0.05790700769059498
1105	4	-0.053756111782362836
1105	5	0.0018711858659301583
1105	6	0.04555245001999708
1105	7	0.04571712094091396
1105	8	0.04403932278266609
1105	9	0.06672134681832631
1105	10-11	0.10464185017347205
1105	12-13	0.02950156002999904
1105	14-15	0.1529390525215959
1105	16-17	0.17732120384473404
1105	18-19	0.13120273534503468
1105	20-21	0.1303945035867713
1105	22-23	0.14612112358440754
1105	24-25	0.12075896978250711
1105	26-27	0.12444869888575028
1105	28-29	0.12084003385478326
1105	30-31	0.14833393007581464
1105	32-33	0.0912411767497403
1105	34-35	0.12728697768760355
1105	36-37	0.09212688390099544
1105	38-39	0.09009416733206166
1105	40-41	0.11209764111378462
1105	42-43	0.07445548321236828
1105	44-45	0.11045830842769533
1105	46-47	0.07044570758263546
1105	48-49	0.03247097151430012
1105	50-51	0.07523052378453343
1105	52-53	0.09415771104292503
1105	54-55	0.08695591756365673
1105	56-57	0.10508209109109146
1105	58-59	0.07781810355649554
1105	60-61	0.06761600358435516
1105	62-63	0.04971760863134023
1105	64-65	0.07840814654785078
1105	66-67	0.05409214003378082
1105	68-69	0.06926914027427244
1105	70-71	0.09274593830676281
1105	72-73	0.08226745691909088
1105	74-75	0.14071932911630824
1105	76-77	0.15987480284663746
1105	78-79	0.1183200944873164
1105	80-81	0.163183399856635
1105	82-83	0.1164440830634561
1105	84-85	0.117492464929839
1105	86-87	0.1240496742138717
1105	88-89	0.12577286776595287
1105	90-91	0.15572305492725036
1105	92-93	0.17803928429655969
1105	94-95	0.15474661288906333
1105	96-97	0.11326406601998684
1105	98-99	0.11635134913996126
1105	100	0.1405771984320836
1106	1	-0.130878403727948
1106	2	-0.11247644412829771
1106	3	-0.09533262373673068
1106	4	-0.13070365002069195
1106	5	-0.14408882000655154
1106	6	-0.037097686460057844
1106	7	-0.019078487644264897
1106	8	0.008123653096667738
1106	9	-0.017012486889562695
1106	10-11	0.03827985322655536
1106	12-13	-0.009382438878311916
1106	14-15	0.08767620889535266
1106	16-17	0.058624930547871656
1106	18-19	0.010507231106210213
1106	20-21	0.03296545232547743
1106	22-23	0.05474526600069396
1106	24-25	0.005828483798467232
1106	26-27	0.05149007966718244
1106	28-29	0.045718574770255316
1106	30-31	0.05043918056507124
1106	32-33	0.01736539165076323
1106	34-35	0.03297502931481944
1106	36-37	0.012504941335336639
1106	38-39	0.06741077201792933
1106	40-41	0.07110111696933785
1106	42-43	0.04149100050391752
1106	44-45	0.0135787807408434
1106	46-47	0.019917897398258333
1106	48-49	0.009865328139042617
1106	50-51	0.03760437082731016
1106	52-53	0.03605248701384056
1106	54-55	0.1040329116735208
1106	56-57	0.05058684457833351
1106	58-59	0.044076904045937226
1106	60-61	0.05518303901095578
1106	62-63	0.06572367851917704
1106	64-65	0.046030133396143924
1106	66-67	0.018233592568627444
1106	68-69	0.01680261346740508
1106	70-71	0.012554456085567267
1106	72-73	0.04348681534550991
1106	74-75	0.019084442100790966
1106	76-77	0.06308242780060169
1106	78-79	0.07857518814897446
1106	80-81	-0.0024729624794161964
1106	82-83	0.05012065886656103
1106	84-85	0.10241398735178109
1106	86-87	0.11095247194914037
1106	88-89	0.08579960570884992
1106	90-91	0.050064228973859315
1106	92-93	0.04644842229423318
1106	94-95	0.05528035357750838
1106	96-97	0.035964271387172175
1106	98-99	0.031170242817566418
1106	100	0.01639330316758958
1107	1	-0.11592386493733642
1107	2	-0.10652811445712729
1107	3	-0.03299015311096554
1107	4	5.721411608874405E-4
1107	5	0.0072238745041275365
1107	6	0.053481999643580025
1107	7	0.044532460462164636
1107	8	0.0764108760491169
1107	9	0.037704646559184596
1107	10-11	0.10322639644859066
1107	12-13	0.0074650310439565715
1107	14-15	0.1353800823601219
1107	16-17	0.0026628846116167892
1107	18-19	0.04039709827964799
1107	20-21	0.0269437259991534
1107	22-23	-0.005901178476108271
1107	24-25	-0.0030238961139517073
1107	26-27	0.024507054353449576
1107	28-29	0.011622458142305447
1107	30-31	-0.0026775646976702205
1107	32-33	-0.018629066248415427
1107	34-35	0.014014687759193123
1107	36-37	0.018020899371862242
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2216	26-27	-0.32845156879235304
2216	28-29	-0.31696528109174693
2216	30-31	-0.33470136054176436
2216	32-33	-0.3372554905599898
2216	34-35	-0.36105505946144234
2216	36-37	-0.3765315391860682
2216	38-39	-0.2894556861267361
2216	40-41	-0.5876856035962206
2216	42-43	-0.7906742468248495
2216	44-45	-0.5031716469380498
2216	46-47	-0.382116969730248
2216	48-49	-0.462066747225947
2216	50-51	-0.44534320713019326
2216	52-53	-0.3829129682725494
2216	54-55	-0.5105564302744057
2216	56-57	-0.38729430396028874
2216	58-59	-0.5151997352942743
2216	60-61	-0.5841105937230964
2216	62-63	-0.49248021211289483
2216	64-65	-0.5042465593949572
2216	66-67	-0.4632314050645405
2216	68-69	-0.5109502884752146
2216	70-71	-0.4928585300520041
2216	72-73	-0.5685750458620618
2216	74-75	-0.44040764580716996
2216	76-77	-0.4710053697706442
2216	78-79	-0.5161724480757641
2216	80-81	-0.4709389963529773
2216	82-83	-0.6135200619603722
2216	84-85	-0.5083407246422951
2216	86-87	-0.3997172670580369
2216	88-89	-0.41139370166683165
2216	90-91	-0.5707985979351307
2216	92-93	-0.8929761605986783
2216	94-95	-0.7961838354130322
2216	96-97	-0.8050652492549304
2216	98-99	-0.6568633731373197
2216	100	-0.5786355663425482
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61251.0
3	10297.0
4	7678.0
5	7315.0
6	8901.0
7	11787.0
8	12337.0
9	12102.0
10	14493.0
11	13990.0
12	14472.0
13	15708.0
14	16403.0
15	16795.0
16	17425.0
17	18355.0
18	18101.0
19	16971.0
20	17557.0
21	18543.0
22	20472.0
23	24459.0
24	33189.0
25	49602.0
26	66157.0
27	71998.0
28	80792.0
29	89713.0
30	111222.0
31	141568.0
32	184598.0
33	248732.0
34	359876.0
35	595676.0
36	1263398.0
37	2721627.0
38	3641508.0
39	1346995.0
40	4323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.902287942249092	12.687356590773902	12.573420616159739	46.83693485081727
2	22.09016564214404	20.176177518319935	33.988614662726775	23.745042176809253
3	25.818830181057535	22.63517521719418	23.60524860629909	27.940745995449195
4	27.498241956714754	29.37930193213966	18.59244085961807	24.530015251527516
5	28.48858755865125	30.91867173395836	21.014332313695373	19.578408393695018
6	22.910962227453012	33.55654382128347	21.975284550026288	21.55720940123723
7	20.291460615128084	17.860966572174455	39.00459234088093	22.842980471816524
8	21.37196921334518	21.658077369137185	28.54904101949459	28.420912398023034
9	21.73976687338683	20.86920824159792	30.71176284667214	26.679262038343104
10-11	25.296096118969512	29.845795870610925	21.27191915280912	23.586188857610445
12-13	23.60706473868896	24.040772233883022	27.044508373372867	25.30765465405515
14-15	23.969994056591396	25.23355611007766	26.368564109971803	24.42788572335914
16-17	24.7629271398756	25.318537211937247	25.35483729839154	24.563698349795608
18-19	24.534419604522167	25.49012952128436	25.231429467865148	24.744021406328322
20-21	24.420692919161628	25.56985688543258	25.47840763219128	24.531042563214516
22-23	24.655083711372512	25.62794287845151	25.434330085068257	24.28264332510772
24-25	24.463723608175588	25.797873882020163	25.41626026027925	24.322142249525
26-27	24.3046499102058	25.83854873072784	25.472767183035472	24.384034176030887
28-29	24.445358087657898	25.52644579171198	25.400596367384054	24.62759975324607
30-31	24.290958091702535	25.614999499608583	25.488902618120807	24.605139790568074
32-33	24.242797495184163	25.78928028612415	25.61131776140384	24.356604457287855
34-35	24.59403863866299	25.699748162660153	25.219966160167388	24.48624703850947
36-37	24.398562051121264	25.583155104694367	25.35222963094212	24.66605321324225
38-39	24.320870870475233	25.687877182951134	25.474595073159552	24.516656873414085
40-41	24.593458888502898	25.574025085183955	25.341995354569512	24.49052067174363
42-43	24.343800105121034	25.488923128288715	25.597837414736407	24.56943935185385
44-45	24.37297565570672	25.615963590300534	25.62975767324977	24.381303080742978
46-47	24.466450278970555	25.619502217508956	25.365657792164576	24.548389711355917
48-49	24.366767983789263	25.49201621777797	25.53451028708395	24.60670551134882
50-51	24.374662101715135	25.624316045086186	25.584435202398794	24.416586650799886
52-53	24.40684069102983	25.66225464585778	25.41093455733173	24.51997010578066
54-55	24.252673103934374	25.565268650440608	25.486734324393765	24.695323921231253
56-57	24.286564034096788	25.597158556926974	25.617844452350035	24.4984329566262
58-59	24.462174871016956	25.56876295000662	25.44669514754222	24.522367031434207
60-61	24.264465347746622	25.64887236135089	25.550382492440903	24.53627979846159
62-63	24.173697908237152	25.78965349328477	25.64929987235222	24.387348726125854
64-65	24.342642012560223	25.69290902099018	25.452997763681367	24.511451202768225
66-67	24.294542908910493	25.61028462092511	25.536517936519164	24.558654533645228
68-69	24.346725989973503	25.73820804694958	25.602481460807425	24.312584502269495
70-71	24.43541127275809	25.643059622989156	25.430566402736616	24.490962701516146
72-73	24.274079325559647	25.692248489431723	25.511427208030735	24.522244976977895
74-75	24.255489061643438	25.831267501867583	25.549346061735022	24.363897374753954
76-77	24.485890533721733	25.618165482623933	25.462651019545767	24.43329296410857
78-79	24.31414807932092	25.51657000899533	25.65531861066031	24.513963301023438
80-81	24.26701757998496	25.738926314140453	25.676896952139096	24.31715915373549
82-83	24.393283423644636	25.620743511059125	25.51643164627684	24.469541419019396
84-85	24.328782737904454	25.615449219491587	25.6315564187321	24.424211623871862
86-87	24.338669242361807	25.78008005315407	25.672122948438847	24.209127756045277
88-89	24.428956818916863	25.69132607833355	25.449577742694256	24.430139360055332
90-91	24.324475773545846	25.69738054661809	25.493254910178564	24.484888769657502
92-93	24.281990548013226	25.839599348095156	25.61731506986228	24.261095034029335
94-95	24.578968084445922	25.787622247340842	25.358825525056584	24.274584143156655
96-97	24.436428775223163	25.733267455459192	25.48954659429954	24.340757175018105
98-99	24.46877899540482	25.84248445486626	25.49287980980268	24.195856739926235
100	24.646027371953753	25.645915209842112	25.228742530773108	24.479314887431023
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	57.0
1	40.5
2	26.0
3	44.5
4	84.0
5	132.0
6	177.0
7	241.0
8	322.0
9	425.5
10	558.5
11	666.5
12	792.5
13	951.0
14	1061.0
15	1197.0
16	1425.0
17	1761.5
18	2285.5
19	3207.5
20	4755.5
21	7149.5
22	10790.5
23	16207.5
24	23359.5
25	32431.5
26	44075.0
27	58035.0
28	72497.0
29	87198.5
30	102849.5
31	119255.0
32	135897.5
33	152913.0
34	169468.5
35	187951.5
36	209251.0
37	231846.0
38	251388.5
39	268476.0
40	284252.5
41	297861.0
42	313556.0
43	331182.5
44	342379.5
45	353175.5
46	366780.5
47	371107.5
48	374389.0
49	377791.0
50	381400.0
51	382575.5
52	374898.5
53	369037.5
54	364984.0
55	358922.0
56	344340.0
57	325866.0
58	313340.0
59	302629.5
60	292089.0
61	277521.0
62	259347.0
63	242303.5
64	223517.5
65	200145.5
66	171773.0
67	146352.0
68	124313.5
69	99073.0
70	73774.5
71	52174.5
72	35727.0
73	23105.0
74	14142.5
75	8085.0
76	4504.5
77	2519.0
78	1448.5
79	789.5
80	483.0
81	317.0
82	234.5
83	174.0
84	137.5
85	123.5
86	98.5
87	70.5
88	45.5
89	33.0
90	31.0
91	27.5
92	25.0
93	27.0
94	26.0
95	21.0
96	15.5
97	13.0
98	15.5
99	14.5
100	31.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.6797854911997538
2	0.5167047735778499
3	0.5117866195648031
4	0.5008525093036543
5	0.5167223384136108
6	0.7197981870630418
7	0.8016151920372275
8	0.8514466310908483
9	0.8849076432153276
10-11	0.37861003482579986
12-13	0.6208730320577573
14-15	0.441742445759348
16-17	0.03067698565637947
18-19	2.9421099899476445E-4
20-21	0.026553640461512543
22-23	0.0
24-25	0.0
26-27	0.005519749637856999
28-29	0.008382817866880677
30-31	0.0026303341551919984
32-33	0.0
34-35	0.03721549576836759
36-37	2.502989095925608E-4
38-39	0.008747288208918968
40-41	0.11468959510067549
42-43	0.09596548017957586
44-45	0.07306093434738643
46-47	0.04223025637809925
48-49	0.17647390488957604
50-51	0.08779344034182576
52-53	0.07259985740866329
54-55	0.11989756890377684
56-57	0.07964335654877676
58-59	0.09792835057585436
60-61	0.1287326812915002
62-63	0.15458811953151771
64-65	0.06121345262667188
66-67	0.07571322454727952
68-69	0.07669685534988889
70-71	0.03636360123396484
72-73	0.07289845961659829
74-75	0.17651781697897823
76-77	0.15799569766912874
78-79	0.1262867779117975
80-81	0.15436416787556648
82-83	0.10399261012229868
84-85	0.19235690762635307
86-87	0.1904642965731181
88-89	0.11043451363760196
90-91	0.08619064907864532
92-93	0.17837090715175122
94-95	0.12790713401073878
96-97	0.17476133340289007
98-99	0.19301558896738613
100	0.16608430453701464
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1386386E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.61912179412018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.19525126886155	50.031800770401716
2	10.474810413149198	12.908972372363175
3	3.4164359243165854	6.315533439668138
4	1.5223359006922546	3.752200051052707
5	0.8286598589619271	2.5530646387636713
6	0.5336535227379907	1.9729956848072123
7	0.36411340257713626	1.5705443670190449
8	0.2603904096613729	1.2836022693555988
9	0.19489630914660913	1.0808405469476468
>10	1.1061643750232706	12.578756317324588
>50	0.07612095609456074	3.221464803547362
>100	0.026967204273352065	2.648544438871461
>500	1.861997880316998E-4	0.06933051485172623
>1k	1.4254716118147184E-5	0.012349785026063556
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.013208756492182857	0.0	0.0	0.0	0.0
2	0.01323510374582418	8.78241788044073E-6	0.0	2.634725364132219E-5	0.0
3	0.013296580670987265	8.78241788044073E-6	0.0	2.634725364132219E-5	0.0
4	0.013603965296802691	8.78241788044073E-6	0.0	2.634725364132219E-5	0.0
5	0.013665442221965775	8.78241788044073E-6	0.0	3.512967152176292E-5	0.0
6	0.013788396072291946	8.78241788044073E-6	0.0	3.512967152176292E-5	0.0
7	0.013858655415335472	3.512967152176292E-5	0.0	4.3912089402203647E-5	0.0
8	0.013990391683542082	5.269450728264438E-5	0.0	4.3912089402203647E-5	0.0
9	0.014113345533868253	5.269450728264438E-5	0.0	4.3912089402203647E-5	0.0
10-11	0.01456124884577073	5.708571622286474E-5	0.0	4.3912089402203647E-5	0.0
12-13	0.015105758754358056	6.586813410330547E-5	0.0	5.269450728264438E-5	0.0
14-15	0.015918132408298823	8.78241788044073E-5	0.0	5.269450728264438E-5	0.0
16-17	0.016914936837728843	9.660659668484803E-5	0.0	5.269450728264438E-5	0.0
18-19	0.01830255886283848	1.0538901456528876E-4	0.0	5.269450728264438E-5	0.0
20-21	0.019641877589605693	1.0538901456528876E-4	0.0	5.269450728264438E-5	0.0
22-23	0.021011934778954446	1.1417143244572948E-4	0.0	5.269450728264438E-5	0.0
24-25	0.022390774386183643	1.3173626820661095E-4	0.0	6.586813410330547E-5	0.0
26-27	0.023756440366592173	1.3173626820661095E-4	0.0	7.904176092396657E-5	4.391208940220365E-6
28-29	0.025337275585071505	1.3612747714683132E-4	0.0	8.782417880440729E-5	1.756483576088146E-5
30-31	0.02674246244594202	1.4051868608705169E-4	0.0	9.660659668484803E-5	1.756483576088146E-5
32-33	0.02813447567999188	1.4051868608705169E-4	0.0	9.660659668484803E-5	1.756483576088146E-5
34-35	0.02983826474879738	1.4490989502727205E-4	0.0	1.0538901456528876E-4	1.756483576088146E-5
36-37	0.0315684010712442	1.624747307881535E-4	0.0	1.0538901456528876E-4	2.1956044701101827E-5
38-39	0.0331843659612453	1.7125714866859422E-4	0.0	1.0978022350550913E-4	2.634725364132219E-5
40-41	0.035068194596599836	1.7564835760881459E-4	0.0	1.229538503261702E-4	2.634725364132219E-5
42-43	0.03706180345545988	1.7564835760881459E-4	0.0	1.229538503261702E-4	2.634725364132219E-5
44-45	0.03916958374676566	1.7564835760881459E-4	0.0	1.3173626820661095E-4	2.634725364132219E-5
46-47	0.04145301239568024	1.8003956654903496E-4	0.0	1.3173626820661095E-4	2.634725364132219E-5
48-49	0.04368813774625241	1.8443077548925533E-4	0.0	1.3173626820661095E-4	2.634725364132219E-5
50-51	0.04598034881304744	1.9321319336969606E-4	0.0	1.3173626820661095E-4	2.634725364132219E-5
52-53	0.04850529395367415	2.0638682019035715E-4	0.0	1.3173626820661095E-4	2.634725364132219E-5
54-55	0.05119271382508901	2.239516559512386E-4	0.0	1.4051868608705169E-4	2.634725364132219E-5
56-57	0.053845004024982115	2.502989095925608E-4	0.0	1.4051868608705169E-4	2.634725364132219E-5
58-59	0.05700228325300055	2.634725364132219E-4	0.0	1.493011039674924E-4	3.073846258154256E-5
60-61	0.060462555897894205	2.634725364132219E-4	0.0	1.7125714866859422E-4	3.512967152176292E-5
62-63	0.06429169009376637	2.634725364132219E-4	0.0	1.8443077548925533E-4	3.512967152176292E-5
64-65	0.06854238034789968	2.7225495429366263E-4	0.0	1.9321319336969606E-4	3.512967152176292E-5
66-67	0.0728194178556743	2.8103737217410337E-4	0.0	1.9760440230991643E-4	3.512967152176292E-5
68-69	0.07779026637600377	2.8103737217410337E-4	0.0	2.1077802913057751E-4	3.512967152176292E-5
70-71	0.08411360724992109	2.898197900545441E-4	0.0	2.239516559512386E-4	3.512967152176292E-5
72-73	0.09167966025392077	2.986022079349848E-4	0.0	2.2834286489145897E-4	3.512967152176292E-5
74-75	0.1007387242975954	3.1177583475564593E-4	0.0	2.2834286489145897E-4	3.512967152176292E-5
76-77	0.11165966093192344	3.24949461576307E-4	0.0	2.2834286489145897E-4	3.512967152176292E-5
78-79	0.1246093360966333	3.24949461576307E-4	0.0	2.2834286489145897E-4	3.512967152176292E-5
80-81	0.1416867476651503	3.24949461576307E-4	0.0	2.2834286489145897E-4	3.512967152176292E-5
82-83	0.1664839045505747	3.24949461576307E-4	0.0	2.371252827718997E-4	3.512967152176292E-5
84-85	0.2015652727739952	3.293406705165274E-4	0.0	2.459077006523404E-4	3.512967152176292E-5
86-87	0.24637316880000382	3.3373187945674775E-4	0.0	2.678637453534423E-4	3.512967152176292E-5
88	0.2853846690249215	3.3373187945674775E-4	0.0	2.7225495429366263E-4	3.512967152176292E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGCG	335	4.8356305E-6	14.117654	6
GCGAACG	595	6.9485395E-10	12.720457	3
CGATCGT	285	0.004137325	11.618266	4
CGCGAGT	810	6.184564E-11	11.100574	1
TACCGTA	860	1.6370905E-11	11.01401	9
CGTATCG	1040	0.0	10.916354	3
TAGATCG	1430	0.0	10.181341	94
CCCGGTT	2900	0.0	10.117437	1
CTCGCGT	935	8.458301E-10	9.616539	1
GTCCGAT	945	1.02591E-9	9.514777	1
GTCGATT	1075	1.1459633E-10	9.24459	1
AGATCGG	8790	0.0	9.190072	94
CGCGGAT	1185	9.094947E-12	9.185151	1
CCGGGAT	3405	0.0	9.172836	1
CGGCGAA	1150	4.5474735E-11	9.05319	1
CGCGTCT	1085	1.3333192E-9	8.723225	1
CTCGGAT	2870	0.0	8.409402	1
CCCGTAT	1580	0.0	8.386442	1
CGGGGTT	3415	0.0	8.314524	1
CGCGATC	860	2.0313018E-6	8.248941	2
>>END_MODULE