FastQCFastQC Report
Wed 25 May 2016
SRR1033286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033286_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11386386
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTT6155.456968E-1213.82543851
CTCGTAT25100.013.67032642-43
CGTATGC27400.012.60626344-45
TATGCCG28800.012.07340246-47
CGCGAAT4355.448317E-611.9449671
TCGTATG23600.011.15336142-43
CGTATCG9500.010.9306293
CGCGGAT13100.010.8176141
CGACGGA6504.153917E-710.1741051
CTCGCGT9004.3655746E-109.9722381
CCGGGTT36550.09.6929371
CCCGGAT39150.09.4111861
CCCGTAT15350.09.231971
CGGGGTT33000.09.16109851
TCGCGTC8251.1686825E-79.1582682
CTCCGAT19650.09.1348741
ATCTCGT39650.09.12879740-41
GTCCGAT8351.3994577E-79.0513851
ATTAGCG6757.879222E-69.0485016
CGCCATT30550.08.9680561
Produced by FastQC (version 0.11.5)