FastQCFastQC Report
Wed 25 May 2016
SRR1033285_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033285_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12302632
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT4204.8920392E-511.2708351
CCCGTAT18450.010.5194461
GATCGGA60600.09.76468994
CGTATCG11700.09.7059153
GTATCGA14000.09.4629174
CGGGAAT28800.09.2045151
CTCCGAT23050.09.0362271
CCGGGAT37600.08.9387311
CTCGGAT33550.08.889011
CGGGGTT39550.08.61771
AGATCGG77050.08.41137594
TCCGATT19700.08.1644952
CGCACAT20450.08.1017731
CCCGGAT45400.07.92434021
GTCGACT13752.1282176E-107.91827341
CCCGGTT31100.07.91495281
TAGATCG14852.0008883E-117.90631494
CCGCAAT21300.07.77846341
CGGGGAT37400.07.7208241
CGATTAG10002.109664E-67.57033444
Produced by FastQC (version 0.11.5)