##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033285_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12302632 Sequences flagged as poor quality 0 Sequence length 100 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14960465370337 34.0 31.0 34.0 30.0 34.0 2 32.371739071769355 34.0 31.0 34.0 31.0 34.0 3 32.42655278967948 34.0 31.0 34.0 31.0 34.0 4 35.78721171209543 37.0 37.0 37.0 35.0 37.0 5 35.770213479522106 37.0 37.0 37.0 35.0 37.0 6 35.70039752469228 37.0 37.0 37.0 35.0 37.0 7 35.66737068945897 37.0 37.0 37.0 35.0 37.0 8 35.65338441400182 37.0 37.0 37.0 35.0 37.0 9 37.39787786873573 39.0 39.0 39.0 35.0 39.0 10-11 37.51138349907565 39.0 39.0 39.0 35.0 39.0 12-13 37.44236164261436 39.0 38.0 39.0 35.0 39.0 14-15 38.928515621697855 41.0 39.0 41.0 36.0 41.0 16-17 39.020917840995324 41.0 39.0 41.0 36.0 41.0 18-19 39.05746376060017 41.0 39.0 41.0 36.0 41.0 20-21 39.01909701111112 41.0 39.0 41.0 36.0 41.0 22-23 38.95713031162762 41.0 39.0 41.0 35.5 41.0 24-25 38.903970995799924 41.0 39.0 41.0 35.0 41.0 26-27 38.82685168507032 41.0 39.0 41.0 35.0 41.0 28-29 38.73169664019862 41.0 39.0 41.0 35.0 41.0 30-31 38.71159065799904 40.5 39.0 41.0 35.0 41.0 32-33 38.78208715826012 41.0 39.0 41.0 35.0 41.0 34-35 38.73073981242388 41.0 39.0 41.0 35.0 41.0 36-37 38.66763315362111 41.0 39.0 41.0 35.0 41.0 38-39 38.57019676765102 41.0 39.0 41.0 34.5 41.0 40-41 38.439494451268644 41.0 38.5 41.0 34.0 41.0 42-43 38.33509305163318 41.0 38.0 41.0 34.0 41.0 44-45 38.19506805535596 40.0 38.0 41.0 33.5 41.0 46-47 38.05596619487602 40.0 38.0 41.0 33.0 41.0 48-49 37.900817239758126 40.0 37.0 41.0 33.0 41.0 50-51 37.71444472207248 40.0 37.0 41.0 33.0 41.0 52-53 37.521209729755384 40.0 36.5 41.0 33.0 41.0 54-55 37.30963817335998 40.0 36.0 41.0 32.5 41.0 56-57 37.0663398693873 39.5 35.0 41.0 32.0 41.0 58-59 36.81633743088471 39.0 35.0 41.0 32.0 41.0 60-61 36.545808734261094 39.0 35.0 41.0 31.0 41.0 62-63 36.24961467594902 38.0 35.0 41.0 31.0 41.0 64-65 35.971668582787814 37.0 35.0 40.0 31.0 41.0 66-67 35.65352556266009 37.0 35.0 40.0 31.0 41.0 68-69 35.33337378538186 36.0 35.0 39.0 30.5 41.0 70-71 35.009671101273284 36.0 35.0 39.0 30.0 41.0 72-73 34.66317821259711 35.0 34.5 39.0 30.0 41.0 74-75 34.335228104034975 35.0 34.0 37.0 30.0 40.0 76-77 34.087510298609274 35.0 34.0 37.0 30.0 39.0 78-79 33.92832960459192 35.0 34.0 36.5 30.0 39.0 80-81 33.6773930570304 35.0 34.0 36.0 30.0 38.5 82-83 33.449436998521946 35.0 34.0 36.0 30.0 37.0 84-85 33.237898849612016 35.0 34.0 35.0 30.0 37.0 86-87 33.04852725823223 35.0 34.0 35.0 29.0 36.5 88-89 32.87498203636425 35.0 34.0 35.0 29.0 36.0 90-91 32.748655816088785 35.0 34.0 35.0 29.0 36.0 92-93 32.59794180627365 35.0 34.0 35.0 29.0 36.0 94-95 32.47020539995019 35.0 34.0 35.0 29.0 35.5 96-97 32.39004397595572 35.0 34.0 35.0 29.0 35.0 98-99 32.29734942083938 35.0 34.0 35.0 29.0 35.0 100 32.20270849359714 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0010266323023770951 1101 2 -8.848516737813839E-4 1101 3 -0.00767633466717399 1101 4 0.03140131672364532 1101 5 0.022221074937860408 1101 6 0.08414406052683887 1101 7 0.16365547497465371 1101 8 0.14174249718215748 1101 9 0.17562235357785028 1101 10-11 0.1648142302294957 1101 12-13 0.12774319444339 1101 14-15 0.2273995473880035 1101 16-17 0.28161701478235557 1101 18-19 0.24700248253411416 1101 20-21 0.2943847676398619 1101 22-23 0.29904287339012825 1101 24-25 0.2858544751043084 1101 26-27 0.16907581445939712 1101 28-29 0.1773358222182182 1101 30-31 0.27453089595226743 1101 32-33 0.18343451256178867 1101 34-35 0.2548258009466693 1101 36-37 0.23714555555885397 1101 38-39 0.2543051994781962 1101 40-41 0.22802791043437765 1101 42-43 0.2050992901790707 1101 44-45 0.15654968499590893 1101 46-47 0.08284464942023817 1101 48-49 0.12845012809154355 1101 50-51 0.16183719044629896 1101 52-53 0.15252706488094248 1101 54-55 0.18837117219994326 1101 56-57 0.15627109543694928 1101 58-59 0.12890511652057057 1101 60-61 0.14365756766862603 1101 62-63 0.16330746626530157 1101 64-65 0.1320677201907543 1101 66-67 0.09082338425778858 1101 68-69 0.1420188282624153 1101 70-71 0.10429090389992979 1101 72-73 0.13702301053069732 1101 74-75 0.09157369088973866 1101 76-77 0.12669757938184745 1101 78-79 0.15123178251457148 1101 80-81 0.14075247133731494 1101 82-83 0.22020886565376685 1101 84-85 0.26374539789267004 1101 86-87 0.1906219148074655 1101 88-89 0.21184143213136508 1101 90-91 0.20132653552487767 1101 92-93 0.20824689735605517 1101 94-95 0.21637029608564973 1101 96-97 0.1635372882724937 1101 98-99 0.13355099554440386 1101 100 0.18562915143817804 1102 1 0.06475421557415473 1102 2 0.07946915865169046 1102 3 0.08066485091895714 1102 4 0.06077195548798642 1102 5 0.074507006546078 1102 6 0.13076322126728712 1102 7 0.18978457721969022 1102 8 0.1877669652523295 1102 9 0.21917204327621675 1102 10-11 0.2587297251204461 1102 12-13 0.17487422267808483 1102 14-15 0.33993666328056804 1102 16-17 0.2984898096491264 1102 18-19 0.29427221876940735 1102 20-21 0.23531036937458794 1102 22-23 0.2693384171885995 1102 24-25 0.3111127955402253 1102 26-27 0.28307685682525374 1102 28-29 0.2867842060370194 1102 30-31 0.3056212677304586 1102 32-33 0.3334950791322484 1102 34-35 0.3625359539593802 1102 36-37 0.33625008236745657 1102 38-39 0.32978511004015587 1102 40-41 0.34155814171560905 1102 42-43 0.3486106441227861 1102 44-45 0.35112007688243807 1102 46-47 0.3626727866124426 1102 48-49 0.369546637919413 1102 50-51 0.30966587665422196 1102 52-53 0.3464570362709338 1102 54-55 0.33926764836621004 1102 56-57 0.3155694207107942 1102 58-59 0.30801295101311155 1102 60-61 0.3749332449752103 1102 62-63 0.3626940554418496 1102 64-65 0.270995649210775 1102 66-67 0.29636923334322773 1102 68-69 0.2511198011543101 1102 70-71 0.23547505862376283 1102 72-73 0.24808060051049097 1102 74-75 0.2691491265480792 1102 76-77 0.338815718322806 1102 78-79 0.3282132926560877 1102 80-81 0.3153289534047161 1102 82-83 0.3122816363912122 1102 84-85 0.3404844636631239 1102 86-87 0.3390953602344666 1102 88-89 0.3242420455895427 1102 90-91 0.28526652057689006 1102 92-93 0.2968630213508163 1102 94-95 0.35146741687297833 1102 96-97 0.3577989693846533 1102 98-99 0.3551965816883822 1102 100 0.3832921483492626 1103 1 -0.02312073888809607 1103 2 0.0027504039932395585 1103 3 -0.03429695527546528 1103 4 0.028912756286054275 1103 5 0.04120446328565919 1103 6 0.12643236875899788 1103 7 0.1364046367077023 1103 8 0.17791691719344271 1103 9 0.23744368010761008 1103 10-11 0.21563276053305458 1103 12-13 0.14065789546241803 1103 14-15 0.30192044676307006 1103 16-17 0.25728979791057327 1103 18-19 0.2537457670965608 1103 20-21 0.23696432553470004 1103 22-23 0.26717219287311167 1103 24-25 0.2561887028298244 1103 26-27 0.2500739447699232 1103 28-29 0.2550385969482889 1103 30-31 0.23610917407953025 1103 32-33 0.22958009193805395 1103 34-35 0.2768194492103362 1103 36-37 0.24202257627962354 1103 38-39 0.2931849772902595 1103 40-41 0.3018438826443699 1103 42-43 0.2931172404301847 1103 44-45 0.28238247207433886 1103 46-47 0.2600371047506016 1103 48-49 0.3316227663796383 1103 50-51 0.28727964894812175 1103 52-53 0.3099702356562659 1103 54-55 0.30105208540378925 1103 56-57 0.29746910352334766 1103 58-59 0.355786085542114 1103 60-61 0.2747739103567426 1103 62-63 0.3125709049248968 1103 64-65 0.2622753200923498 1103 66-67 0.2840979395371477 1103 68-69 0.2772746527169758 1103 70-71 0.2774156583014573 1103 72-73 0.2948885336619611 1103 74-75 0.2830131373695153 1103 76-77 0.3292835696500518 1103 78-79 0.33460049684778426 1103 80-81 0.28385899841745044 1103 82-83 0.320382096413077 1103 84-85 0.32674780116132496 1103 86-87 0.3153149527608221 1103 88-89 0.33856629096649726 1103 90-91 0.3218633144608205 1103 92-93 0.41967616807746566 1103 94-95 0.4142988721662775 1103 96-97 0.3662431377254407 1103 98-99 0.3552935127349315 1103 100 0.3534820440403408 1104 1 -0.05319257770931074 1104 2 -0.019859737964623037 1104 3 -0.023323442122823224 1104 4 0.00825859475992985 1104 5 -0.009836538493885882 1104 6 0.05966401900224838 1104 7 0.07357875274145442 1104 8 0.06946337320884055 1104 9 0.1268799676636334 1104 10-11 0.13084401983959992 1104 12-13 0.04254367509166457 1104 14-15 0.16907990520758176 1104 16-17 0.15591572997423953 1104 18-19 0.15256979793078074 1104 20-21 0.1704190324844035 1104 22-23 0.17672546485075458 1104 24-25 0.14631636961451733 1104 26-27 0.19228637098859735 1104 28-29 0.15210298405299483 1104 30-31 0.1502842667220392 1104 32-33 0.10706364267772983 1104 34-35 0.17572277311965223 1104 36-37 0.15945693852930276 1104 38-39 0.18573196389966284 1104 40-41 0.14067155884556826 1104 42-43 0.15800382807349678 1104 44-45 0.17167584179180295 1104 46-47 0.14796776834835867 1104 48-49 0.08756720963370412 1104 50-51 0.12195119800072263 1104 52-53 0.12274486457877032 1104 54-55 0.12450233220612716 1104 56-57 0.09003459889856202 1104 58-59 0.10994289637322652 1104 60-61 0.09360793987153215 1104 62-63 0.09946539481034478 1104 64-65 0.025796128568771337 1104 66-67 0.050587706290045276 1104 68-69 0.024683207008976638 1104 70-71 0.040462505636988055 1104 72-73 0.04701205859932145 1104 74-75 0.07542327470371646 1104 76-77 0.06800960270137324 1104 78-79 0.02963762433243744 1104 80-81 0.06474503263033426 1104 82-83 0.08168900699135406 1104 84-85 0.090561063643527 1104 86-87 0.1043154949140046 1104 88-89 0.13972265183583232 1104 90-91 0.13634271630487405 1104 92-93 0.18685422691532239 1104 94-95 0.23810352729707773 1104 96-97 0.09879501019793224 1104 98-99 0.1071974275714922 1104 100 0.12637414512499134 1105 1 -0.10010608490693329 1105 2 -0.10929223854219572 1105 3 -0.061280567195744595 1105 4 -0.03968638053330409 1105 5 0.016692114875056063 1105 6 0.05160876492891475 1105 7 0.05384298967378953 1105 8 0.04893368602827053 1105 9 0.06548839405940043 1105 10-11 0.120014610392019 1105 12-13 -0.008047292851856014 1105 14-15 0.14324637381012195 1105 16-17 0.19124892646326686 1105 18-19 0.15653673424166925 1105 20-21 0.14353674739506062 1105 22-23 0.17792183587278743 1105 24-25 0.15451924101799364 1105 26-27 0.16062701191856377 1105 28-29 0.17702472517792245 1105 30-31 0.22785800059366323 1105 32-33 0.15542343904010636 1105 34-35 0.1662875882884265 1105 36-37 0.11390661666817437 1105 38-39 0.10231464306736626 1105 40-41 0.15484922984416016 1105 42-43 0.1157107358299001 1105 44-45 0.1300383390285944 1105 46-47 0.08920513448886425 1105 48-49 0.10899631292677725 1105 50-51 0.13748654455199727 1105 52-53 0.1534602057116743 1105 54-55 0.20446062239472695 1105 56-57 0.17108477366590336 1105 58-59 0.1390189729417699 1105 60-61 0.17269240161634514 1105 62-63 0.1235697100597335 1105 64-65 0.12087870053252914 1105 66-67 0.1310689190075962 1105 68-69 0.13971222686580376 1105 70-71 0.14054447094280675 1105 72-73 0.14309210088254787 1105 74-75 0.1506721789797396 1105 76-77 0.17770086366900273 1105 78-79 0.14618335553809203 1105 80-81 0.1777397871166997 1105 82-83 0.14707330402438146 1105 84-85 0.17024420536713336 1105 86-87 0.16779208539662704 1105 88-89 0.18725295564681232 1105 90-91 0.2297096845276272 1105 92-93 0.22512491104265564 1105 94-95 0.209395068823234 1105 96-97 0.21988788371224643 1105 98-99 0.17382256448505018 1105 100 0.2522827774520451 1106 1 -0.07180400234904738 1106 2 -0.023154043425691384 1106 3 -0.027271519204482786 1106 4 -0.011688461850454246 1106 5 -0.029046845769130414 1106 6 0.05760123682648555 1106 7 0.06214618888468948 1106 8 0.09495872697220875 1106 9 0.09098559932503747 1106 10-11 0.13816728632690456 1106 12-13 0.09776972494014302 1106 14-15 0.17127172803726864 1106 16-17 0.16606893595481864 1106 18-19 0.1444108595301401 1106 20-21 0.15983779549799237 1106 22-23 0.17026499265086414 1106 24-25 0.08375088293218624 1106 26-27 0.14485587770927566 1106 28-29 0.11517361689980277 1106 30-31 0.1551168810869754 1106 32-33 0.12651644072749946 1106 34-35 0.1620121875340672 1106 36-37 0.12306164377802986 1106 38-39 0.2084329400474374 1106 40-41 0.19755436055459796 1106 42-43 0.1820747740194406 1106 44-45 0.17922758690075824 1106 46-47 0.1776583606855482 1106 48-49 0.1690539989115436 1106 50-51 0.19869231268520338 1106 52-53 0.1963562763337734 1106 54-55 0.2538354052677505 1106 56-57 0.18477984829555538 1106 58-59 0.17713809500435218 1106 60-61 0.15680729125379855 1106 62-63 0.1910687329883345 1106 64-65 0.1883183547423144 1106 66-67 0.14997033405511218 1106 68-69 0.18531923634494518 1106 70-71 0.17272089746703756 1106 72-73 0.17377623696532396 1106 74-75 0.20214164782512256 1106 76-77 0.204742896349714 1106 78-79 0.21239939067544356 1106 80-81 0.16017868138206381 1106 82-83 0.21437275624403895 1106 84-85 0.21360432286385134 1106 86-87 0.23723178148090085 1106 88-89 0.2481785834539494 1106 90-91 0.15876103350651505 1106 92-93 0.19511807929745117 1106 94-95 0.17480335283772064 1106 96-97 0.20052543054963934 1106 98-99 0.1843610006124976 1106 100 0.14057712098414044 1107 1 -0.09856906554943379 1107 2 -0.04324655364928276 1107 3 0.00693806662943075 1107 4 0.04380411830072717 1107 5 0.04782432591639463 1107 6 0.10567950040925211 1107 7 0.09862532346432573 1107 8 0.11615359260500924 1107 9 0.07135259748390865 1107 10-11 0.14054131154509264 1107 12-13 0.04611005103286914 1107 14-15 0.19826079189267887 1107 16-17 0.028280899548207117 1107 18-19 0.09923503367640762 1107 20-21 0.12252647276001483 1107 22-23 0.1017123278784382 1107 24-25 0.06984051970702154 1107 26-27 0.1293767353844899 1107 28-29 0.1254797769454754 1107 30-31 0.13265723329190848 1107 32-33 0.11947350385706557 1107 34-35 0.16220376922542812 1107 36-37 0.1237747779947469 1107 38-39 0.13440163638465208 1107 40-41 0.13866567267066188 1107 42-43 0.12509933055456912 1107 44-45 0.1768697435031541 1107 46-47 0.19903570746445354 1107 48-49 0.19348177100955155 1107 50-51 0.21643168192060358 1107 52-53 0.2198936610417661 1107 54-55 0.2321875593031919 1107 56-57 0.2590931582164231 1107 58-59 0.2578183842744437 1107 60-61 0.2235420322118955 1107 62-63 0.22256685423747768 1107 64-65 0.19901279549859652 1107 66-67 0.20624185449344168 1107 68-69 0.18029269262692793 1107 70-71 0.20612922039973824 1107 72-73 0.21108570640077318 1107 74-75 0.19227405660944186 1107 76-77 0.2153208279977079 1107 78-79 0.16110617276689254 1107 80-81 0.11690456521373704 1107 82-83 0.2021725486741559 1107 84-85 0.16382832351886378 1107 86-87 0.10611795772499022 1107 88-89 0.1502010067548838 1107 90-91 0.10928103700289427 1107 92-93 0.198210853962415 1107 94-95 0.2572919398134914 1107 96-97 0.17196200002579332 1107 98-99 0.163425790962485 1107 100 0.21250926908901846 1108 1 -0.1219969607819067 1108 2 -0.13544203796210752 1108 3 -0.09861388872374022 1108 4 -0.05086399997197333 1108 5 -0.07294707707901438 1108 6 -0.013172810478792485 1108 7 0.024829023220135582 1108 8 0.02252467088128185 1108 9 -0.016520904120582713 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-0.15019337236985564 2214 64-65 -0.12965441948740875 2214 66-67 -0.12059244036468186 2214 68-69 -0.11253357723015966 2214 70-71 -0.08609109646008761 2214 72-73 -0.1311291060237778 2214 74-75 -0.10244561767068916 2214 76-77 -0.15832845517373073 2214 78-79 -0.1475208256291296 2214 80-81 -0.20120059291418357 2214 82-83 -0.24251108221179862 2214 84-85 -0.13302108579593863 2214 86-87 -0.1715790244817299 2214 88-89 -0.15263772634818196 2214 90-91 -0.11620537679741716 2214 92-93 -0.1508754807470254 2214 94-95 -0.18080571061858564 2214 96-97 -0.19122629437480754 2214 98-99 -0.19537063226845675 2214 100 -0.17692527870413244 2215 1 -0.14224578120764875 2215 2 -0.2614269426008917 2215 3 -0.26934137563578986 2215 4 -0.15344116031305788 2215 5 -0.1214810177679908 2215 6 -0.19682961256508946 2215 7 -0.2700258115221601 2215 8 -0.3439115017883765 2215 9 -0.43241593530034805 2215 10-11 -0.41864904792492297 2215 12-13 -0.3740099196210096 2215 14-15 -0.5023171655776508 2215 16-17 -0.3055902140063438 2215 18-19 -0.3585550991561064 2215 20-21 -0.35097784217754224 2215 22-23 -0.35643880677213957 2215 24-25 -0.32330470678437706 2215 26-27 -0.28726840645069984 2215 28-29 -0.3434666838917124 2215 30-31 -0.3103972369118111 2215 32-33 -0.31425611940615283 2215 34-35 -0.3282585140353831 2215 36-37 -0.3396100152756176 2215 38-39 -0.3030699695455823 2215 40-41 -0.34842555046240165 2215 42-43 -0.3066835710262339 2215 44-45 -0.3315292771497482 2215 46-47 -0.28871312792265513 2215 48-49 -0.39916453417890807 2215 50-51 -0.30089498126999814 2215 52-53 -0.28682171432100745 2215 54-55 -0.326130975679348 2215 56-57 -0.3146398705157054 2215 58-59 -0.34030590149427553 2215 60-61 -0.35718377885382324 2215 62-63 -0.27429759371211304 2215 64-65 -0.27384680318940724 2215 66-67 -0.28630083292593156 2215 68-69 -0.3553995394684435 2215 70-71 -0.31403585023056735 2215 72-73 -0.34711758446457 2215 74-75 -0.32793230176248045 2215 76-77 -0.3166623769794228 2215 78-79 -0.28819100396893305 2215 80-81 -0.28916303860659553 2215 82-83 -0.33573862661401677 2215 84-85 -0.3340833751672463 2215 86-87 -0.3193720738839687 2215 88-89 -0.34057945337212914 2215 90-91 -0.3115343988331176 2215 92-93 -0.37810301201569274 2215 94-95 -0.35542503403335246 2215 96-97 -0.3853354576159589 2215 98-99 -0.512033812513959 2215 100 -0.5325274055289064 2216 1 -0.02050967087329525 2216 2 -0.10053575418344707 2216 3 -0.11802440162772854 2216 4 -0.14749732558654927 2216 5 -0.07949177169940214 2216 6 -0.07656639466214443 2216 7 -0.13418311044852516 2216 8 -0.30906076318406406 2216 9 -0.3964574545401547 2216 10-11 -0.2505494207296266 2216 12-13 -0.18607545796864144 2216 14-15 -0.3577836900942657 2216 16-17 -0.3996326065090159 2216 18-19 -0.38289889178570036 2216 20-21 -0.37435386833676887 2216 22-23 -0.3426005694992824 2216 24-25 -0.29046443715161274 2216 26-27 -0.35729601749083173 2216 28-29 -0.33715439946953296 2216 30-31 -0.34352447224540583 2216 32-33 -0.31544849046390766 2216 34-35 -0.3854255421697701 2216 36-37 -0.3874238223151849 2216 38-39 -0.285418406243771 2216 40-41 -0.6395226972022456 2216 42-43 -0.8337470383706247 2216 44-45 -0.5848839809523341 2216 46-47 -0.4477240482156901 2216 48-49 -0.5183247338815988 2216 50-51 -0.5036756219579175 2216 52-53 -0.4817640124561109 2216 54-55 -0.5997691549954709 2216 56-57 -0.4433214251283104 2216 58-59 -0.5775181799982292 2216 60-61 -0.6100482483672138 2216 62-63 -0.5656324635790568 2216 64-65 -0.5592877272206067 2216 66-67 -0.5232414453531788 2216 68-69 -0.5950116449011276 2216 70-71 -0.5508659688269901 2216 72-73 -0.6606879749609433 2216 74-75 -0.5382367933575267 2216 76-77 -0.5228340603148709 2216 78-79 -0.5799728802567259 2216 80-81 -0.5233379446030781 2216 82-83 -0.7147182652761472 2216 84-85 -0.5567307864757396 2216 86-87 -0.4367062310837042 2216 88-89 -0.4746882168295059 2216 90-91 -0.6169291446623717 2216 92-93 -0.9278847592360435 2216 94-95 -0.7955186119198814 2216 96-97 -0.8272116061194339 2216 98-99 -0.6458888739277207 2216 100 -0.5657358582280132 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58849.0 3 11254.0 4 8888.0 5 8418.0 6 10293.0 7 13168.0 8 14208.0 9 14160.0 10 16748.0 11 16321.0 12 16638.0 13 18003.0 14 18800.0 15 19512.0 16 20108.0 17 20960.0 18 20741.0 19 19892.0 20 20221.0 21 21237.0 22 23414.0 23 28328.0 24 37839.0 25 55737.0 26 74054.0 27 81085.0 28 91915.0 29 101498.0 30 125681.0 31 160227.0 32 207436.0 33 277407.0 34 401460.0 35 664559.0 36 1402190.0 37 2934750.0 38 3854125.0 39 1408058.0 40 4450.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.812928771100935 12.432701692788369 11.849952302277453 48.90441723383325 2 21.666131919935218 19.552832445996913 34.90284154363611 23.87819409043176 3 25.877635299657747 22.049059146593134 23.395675090627872 28.677630463121247 4 27.58373032006537 28.838060536729166 18.462801369070185 25.115407774135278 5 28.782965311849555 30.49229865738364 20.89973891482819 19.824997115938615 6 23.21446918421521 33.159859761725926 21.887052110430478 21.73861894362839 7 20.50578492895119 17.89681105842774 38.61108448265832 22.986319529962746 8 21.305819838637486 21.462255566613273 28.752514628703903 28.47940996604534 9 21.73319192579696 20.63414276929324 30.924033959129744 26.70863134578005 10-11 25.34314330230879 29.5662185960563 21.357635346436354 23.73300275519855 12-13 23.732010385847687 23.939839390026922 26.861284353065823 25.466865871059568 14-15 24.12484282714141 25.084996420365442 26.20252085630051 24.58763989619264 16-17 24.916090019099915 25.151882360684674 25.15570388866084 24.776323731554573 18-19 24.70726202332475 25.328114652210644 25.0214720915644 24.943151232900206 20-21 24.595390792202863 25.36485096717002 25.327328619420708 24.71242962120641 22-23 24.79430417816285 25.458609182165247 25.289933893820443 24.457152745851456 24-25 24.62400322142449 25.590617519893303 25.26657710317597 24.518802155506236 26-27 24.46367180712755 25.62828747156184 25.3489975644031 24.559043156907514 28-29 24.610789933195708 25.32865532710391 25.24219152352327 24.818363216177108 30-31 24.461194830754305 25.432286785522408 25.31053755520344 24.795980828519852 32-33 24.44740279966108 25.586390782070044 25.425856028205995 24.540350390062873 34-35 24.787921938295014 25.4910040326666 25.08484667426569 24.636227354772693 36-37 24.58664342172424 25.375317884924286 25.150499636072997 24.88753905727848 38-39 24.518599535236692 25.50628557966832 25.2953118313205 24.67980305377449 40-41 24.760590309946657 25.377703823434654 25.171924724439105 24.689781142179584 42-43 24.524280867877252 25.32408384309306 25.405831717190676 24.745803571839012 44-45 24.532983442208625 25.432332207745915 25.457133470243242 24.577550879802214 46-47 24.663490410038793 25.434196151330006 25.177318632778462 24.724994805852738 48-49 24.571108566998994 25.32820403417289 25.328749588798377 24.771937810029737 50-51 24.563929992545493 25.460712269576746 25.412204680305088 24.56315305757267 52-53 24.634560936471473 25.48984519238649 25.188339739336246 24.687254131805787 54-55 24.469297202477797 25.379629721936432 25.28133405735019 24.86973901823558 56-57 24.495234881593294 25.422853492178344 25.40158091451991 24.680330711708457 58-59 24.693786549377513 25.34755178336574 25.269191406915365 24.689470260341388 60-61 24.50292564004493 25.40116177548907 25.354787443722394 24.741125140743613 62-63 24.403696208572338 25.553859596843036 25.500907640252162 24.541536554332456 64-65 24.56721472674059 25.433094337336122 25.289546273602227 24.710144662321063 66-67 24.519015832834423 25.367541550615613 25.347067216336516 24.766375400213448 68-69 24.536655465919935 25.515141344486576 25.4565481092517 24.491655080341797 70-71 24.638675713951113 25.440708315903514 25.261977869234332 24.658638100911045 72-73 24.499262071277077 25.47852502757243 25.309871292882764 24.712341608267728 74-75 24.47783350749265 25.61695188325352 25.350326494774283 24.55488811447955 76-77 24.71234774164524 25.424000715121114 25.2481634722582 24.615488070975445 78-79 24.542832393316026 25.314194447809534 25.452241707387905 24.690731451486524 80-81 24.477092487899416 25.535283768363865 25.48534727376169 24.502276469975033 82-83 24.59005822255639 25.424158823423394 25.3251460181551 24.660636935865114 84-85 24.550354852975985 25.385505671649618 25.451524356488846 24.61261511888555 86-87 24.557076791109704 25.57618842744192 25.482436948832394 24.384297832615985 88-89 24.624479239606213 25.512122588321052 25.245062637719695 24.618335534353037 90-91 24.51772447592038 25.477022046747443 25.318876718276574 24.686376759055598 92-93 24.470380689135155 25.62976380523836 25.474097480163067 24.425758025463416 94-95 24.775437591136633 25.575244506510845 25.197425652265938 24.451892250086587 96-97 24.643657372372203 25.48249785975391 25.318170766640463 24.55567400123342 98-99 24.645386121674406 25.628135199176448 25.349411917860415 24.37706676128873 100 24.837878927399217 25.398236637941668 25.061598639281318 24.7022857953778 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 59.0 1 41.0 2 24.5 3 42.0 4 73.0 5 118.0 6 167.0 7 238.0 8 332.0 9 467.0 10 595.5 11 688.5 12 827.0 13 941.5 14 1061.5 15 1222.0 16 1443.5 17 1826.5 18 2389.5 19 3304.0 20 4832.0 21 7329.0 22 11106.5 23 16533.5 24 23866.0 25 33527.0 26 45824.0 27 59661.0 28 74614.0 29 89943.5 30 105959.0 31 123315.5 32 140917.0 33 158684.5 34 176653.5 35 196295.0 36 219100.5 37 243526.0 38 263899.0 39 282925.5 40 300322.0 41 314890.0 42 331317.0 43 350176.5 44 362959.5 45 376118.5 46 391078.0 47 395063.0 48 399398.5 49 404549.0 50 409310.0 51 410986.0 52 403143.5 53 398028.5 54 394277.0 55 387697.0 56 373317.0 57 353411.0 58 340445.0 59 330870.5 60 319665.0 61 305105.5 62 287273.5 63 268436.5 64 247853.5 65 223514.0 66 194176.0 67 166986.5 68 143969.5 69 117830.0 70 90997.0 71 67784.5 72 49455.5 73 35129.5 74 24100.5 75 15609.0 76 9590.5 77 5673.0 78 3219.5 79 1651.0 80 899.0 81 542.0 82 345.5 83 255.0 84 181.0 85 127.5 86 98.5 87 78.0 88 57.0 89 46.5 90 43.0 91 32.5 92 27.5 93 24.0 94 18.0 95 13.5 96 12.5 97 17.0 98 17.5 99 15.5 100 34.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6744166614103388 2 0.5136543139711892 3 0.5077612660445342 4 0.49614586537254796 5 0.5116628701890782 6 0.7129124889698399 7 0.7936675664199335 8 0.8454451047548199 9 0.8786006116414764 10-11 0.37819549507780126 12-13 0.6177052195009979 14-15 0.4403529261055683 16-17 0.03159080105785494 18-19 2.804277978891021E-4 20-21 0.026782886783901203 22-23 0.0 24-25 0.0 26-27 0.005848342045832144 28-29 0.007742245724329558 30-31 0.002401925051484918 32-33 0.0 34-35 0.03711400942497508 36-37 2.2352940411450166E-4 38-39 0.008953368677531767 40-41 0.11354480894819904 42-43 0.09585347265528221 44-45 0.07236662853932394 46-47 0.041755292688588914 48-49 0.17518202609002692 50-51 0.08730652107614045 52-53 0.07172855369485164 54-55 0.11952320446551601 56-57 0.0797430988751025 58-59 0.09738972928719644 60-61 0.12731015607066848 62-63 0.15326395197385406 64-65 0.06053176263420705 66-67 0.07471571936801816 68-69 0.07643892786519177 70-71 0.03660598805198757 72-73 0.07397197607796446 74-75 0.17663699930226312 76-77 0.15767357749138558 78-79 0.12627785664075783 80-81 0.15468641181821907 82-83 0.10410373975259929 84-85 0.19128020735725496 86-87 0.18986994002584162 88-89 0.11079742936308264 90-91 0.08757069219009396 92-93 0.17782373722956193 94-95 0.12835871218451467 96-97 0.1734100475410465 98-99 0.19188983300483994 100 0.16499721360437344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2302632E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.43048264487477 #Duplication Level Percentage of deduplicated Percentage of total 1 80.60228895277683 48.70835223697962 2 10.76356108728228 13.008943829641225 3 3.506493345826642 6.356972558380372 4 1.5570392782391973 3.7637054032408863 5 0.8912317406963759 2.692878211935694 6 0.569462705375796 2.064774368046929 7 0.35351819067933665 1.495429242054663 8 0.26411782072681045 1.2768613905306931 9 0.20612835780604868 1.1210792534113465 >10 1.1740849180676094 13.035583177025668 >50 0.0807320339942535 3.3572568910242353 >100 0.031097615302982648 3.0244976177325187 >500 2.3049621108200545E-4 0.08454156005982126 >1k 1.3457014779438317E-5 0.009124259936465125 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.004494973108193434 8.12834196780006E-6 0.0 0.0 0.0 2 0.004519358134096834 3.251336787120024E-5 0.0 4.06417098390003E-5 0.0 3 0.004584384869839234 3.251336787120024E-5 0.0 4.877005180680037E-5 0.0 4 0.004746951709195236 3.251336787120024E-5 0.0 6.502673574240049E-5 0.0 5 0.004836363470841036 3.251336787120024E-5 0.0 6.502673574240049E-5 0.0 6 0.005031443678068238 3.251336787120024E-5 0.0 7.315507771020055E-5 0.0 7 0.005088342071842838 4.06417098390003E-5 0.0 7.315507771020055E-5 0.0 8 0.00527529393710224 4.06417098390003E-5 0.0 7.315507771020055E-5 0.0 9 0.005454117460393841 4.06417098390003E-5 0.0 7.315507771020055E-5 0.0 10-11 0.006014973056172045 4.877005180680037E-5 0.0 7.315507771020055E-5 0.0 12-13 0.006616470361789249 5.28342227907004E-5 8.12834196780006E-6 8.12834196780006E-5 0.0 14-15 0.007445561242504856 6.096256475850045E-5 8.12834196780006E-6 8.534759066190064E-5 0.0 16-17 0.008575400776029064 6.502673574240049E-5 8.12834196780006E-6 9.754010361360074E-5 0.0 18-19 0.009851550464973675 6.502673574240049E-5 8.12834196780006E-6 9.754010361360074E-5 0.0 20-21 0.011054545076208082 6.909090672630052E-5 8.12834196780006E-6 1.0566844558140079E-4 0.0 22-23 0.012440427381717994 8.534759066190064E-5 8.12834196780006E-6 1.0566844558140079E-4 0.0 24-25 0.013830373858211804 9.754010361360074E-5 8.12834196780006E-6 1.219251295170009E-4 0.0 26-27 0.015244705360609016 9.754010361360074E-5 8.12834196780006E-6 1.3005347148480097E-4 0.0 28-29 0.016707806914813023 9.754010361360074E-5 8.12834196780006E-6 1.3005347148480097E-4 0.0 30-31 0.018085560878355136 9.754010361360074E-5 8.12834196780006E-6 1.3411764246870102E-4 0.0 32-33 0.019479571525832844 9.754010361360074E-5 8.12834196780006E-6 1.3818181345260104E-4 0.0 34-35 0.021117432432344558 1.0566844558140079E-4 1.2192512951700092E-5 1.3818181345260104E-4 0.0 36-37 0.02271871580000117 1.2598930050090095E-4 1.625668393560012E-5 1.3818181345260104E-4 0.0 38-39 0.02421026655109248 1.3818181345260104E-4 1.625668393560012E-5 1.3818181345260104E-4 0.0 40-41 0.025905025851378796 1.3818181345260104E-4 1.625668393560012E-5 1.463101554204011E-4 0.0 42-43 0.02780299370086011 1.5037432640430115E-4 1.625668393560012E-5 1.5037432640430115E-4 4.06417098390003E-6 44-45 0.029721282405260924 1.7882352329160134E-4 1.625668393560012E-5 1.7069518132380127E-4 1.625668393560012E-5 46-47 0.03167614864851684 1.869518652594014E-4 1.625668393560012E-5 1.7882352329160134E-4 1.625668393560012E-5 48-49 0.03367978494357955 1.869518652594014E-4 1.625668393560012E-5 1.7882352329160134E-4 1.625668393560012E-5 50-51 0.035951656523579664 1.9508020722720147E-4 1.625668393560012E-5 1.869518652594014E-4 1.625668393560012E-5 52-53 0.03815443719685349 1.9508020722720147E-4 1.625668393560012E-5 1.9101603624330145E-4 1.625668393560012E-5 54-55 0.040621388984080804 1.991443782111015E-4 1.625668393560012E-5 1.9508020722720147E-4 1.625668393560012E-5 56-57 0.04333625520132603 2.1946523313060164E-4 1.625668393560012E-5 1.991443782111015E-4 1.625668393560012E-5 58-59 0.04596577382790935 2.479144300179019E-4 1.625668393560012E-5 2.1540106214670162E-4 2.032085491950015E-5 60-61 0.04911144216944797 2.519786010018019E-4 1.625668393560012E-5 2.3572191706620177E-4 2.4385025903400184E-5 62-63 0.05257411584773079 2.6823528493740204E-4 1.625668393560012E-5 2.3572191706620177E-4 2.4385025903400184E-5 64-65 0.05615465048454672 2.804277978891021E-4 1.625668393560012E-5 2.4385025903400184E-4 2.4385025903400184E-5 66-67 0.06003186960318735 3.048128237925023E-4 1.625668393560012E-5 2.4385025903400184E-4 2.4385025903400184E-5 68-69 0.06434395501710528 3.0887699477640234E-4 1.625668393560012E-5 2.560427719857019E-4 2.4385025903400184E-5 70-71 0.06954609387649732 3.1294116576030236E-4 1.625668393560012E-5 2.6010694296960195E-4 2.4385025903400184E-5 72-73 0.07613411504139928 3.170053367442024E-4 1.625668393560012E-5 2.763636269052021E-4 2.4385025903400184E-5 74-75 0.08403079926311703 3.251336787120024E-4 1.625668393560012E-5 2.9262031084080215E-4 2.4385025903400184E-5 76-77 0.0930126171375361 3.251336787120024E-4 1.625668393560012E-5 3.0074865280860225E-4 2.4385025903400184E-5 78-79 0.10352662747288548 3.332620206798025E-4 1.625668393560012E-5 3.170053367442024E-4 2.4385025903400184E-5 80-81 0.11687336498401318 3.332620206798025E-4 1.625668393560012E-5 3.2919784969590244E-4 2.4385025903400184E-5 82-83 0.1359424552404721 3.4139036264760255E-4 1.625668393560012E-5 3.332620206798025E-4 2.4385025903400184E-5 84-85 0.16315207997768283 3.617112175671027E-4 1.625668393560012E-5 3.3732619166370253E-4 2.4385025903400184E-5 86-87 0.19681560823732677 3.739037305188028E-4 1.625668393560012E-5 3.6983955953490274E-4 2.4385025903400184E-5 88 0.2271871580000117 3.739037305188028E-4 1.625668393560012E-5 3.739037305188028E-4 2.4385025903400184E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGAAT 420 4.8920392E-5 11.270835 1 CCCGTAT 1845 0.0 10.519446 1 GATCGGA 6060 0.0 9.764689 94 CGTATCG 1170 0.0 9.705915 3 GTATCGA 1400 0.0 9.462917 4 CGGGAAT 2880 0.0 9.204515 1 CTCCGAT 2305 0.0 9.036227 1 CCGGGAT 3760 0.0 8.938731 1 CTCGGAT 3355 0.0 8.88901 1 CGGGGTT 3955 0.0 8.6177 1 AGATCGG 7705 0.0 8.411375 94 TCCGATT 1970 0.0 8.164495 2 CGCACAT 2045 0.0 8.101773 1 CCCGGAT 4540 0.0 7.9243402 1 GTCGACT 1375 2.1282176E-10 7.9182734 1 CCCGGTT 3110 0.0 7.9149528 1 TAGATCG 1485 2.0008883E-11 7.906314 94 CCGCAAT 2130 0.0 7.7784634 1 CGGGGAT 3740 0.0 7.720824 1 CGATTAG 1000 2.109664E-6 7.5703344 4 >>END_MODULE