FastQCFastQC Report
Wed 25 May 2016
SRR1033285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033285_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12302632
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC4254.205631E-612.2252111
CCCGTAT17100.09.9439521
CGCGGAT13050.09.410561
CGCCTAT9105.5861165E-99.3429431
GATCGGA63750.09.06459794
CGTATCG11504.9112714E-119.0280573
CCCGGTT30600.08.95280551
CTCCGAT23500.08.6427761
CTCCGGT27500.08.5879581
AGATCGG79300.08.41276594
CGACCTA7352.1801228E-58.3470764
CGTTAAT10901.3789759E-88.233411
GTATCGA15001.8189894E-128.1801354
CGATAAC15253.6379788E-128.0529711
CCCGGAT43100.08.0001521
CCCGCTT47050.07.93085581
CCGGGAT37650.07.9036591
CGCGAGT8451.4487621E-57.8257131
CGGGCAT39850.07.82290841
CTGGAAT131400.07.8004021
Produced by FastQC (version 0.11.5)