FastQCFastQC Report
Wed 25 May 2016
SRR1033284_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033284_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11022852
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA3650.00218100710.3737371
CTCCGAT20950.010.1663871
CCCGGTT26700.010.1041961
CCCGTAT14550.010.0840931
TCCGATT18950.09.9855992
TCGCGCG3850.00337103619.8430958
CTCGGAT30700.09.55853751
GTCCGAT11252.910383E-119.255681
AGATCGG71200.08.97142294
CGACCGT9601.3485987E-88.8744091
CCGGGAT32550.08.8698641
CTCGGTT19800.08.8445281
GTTCGAT14650.08.7229681
GATCGGA56100.08.62336494
CGACGGA7251.7695273E-58.4867911
CGCGGAT12351.9826984E-108.4312871
CTCCGGT29350.08.3855861
CCGGAAT22700.08.340121
CGCCCTT31500.08.2641
GTCGTTA9902.0830885E-78.1274041
Produced by FastQC (version 0.11.5)