FastQCFastQC Report
Wed 25 May 2016
SRR1033284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033284_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11022852
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG9600.014.2585913
CGCTAAT5452.6066118E-913.002141
CGCGTTT7700.012.883941
GTCCGAT10100.012.6288121
CCCGTAT15700.012.0359521
CGTATGC22750.011.67142644-45
TCGTATG20150.011.54712142-43
TATGCCG25100.010.76462246-47
TCCGATT19900.010.678622
CTCGTAT23150.010.35530342-43
GTATCGA13950.09.8125424
CCCGGTT26750.09.3599211
CTCGGAT31750.08.9274541
CCGCGAT14450.08.8270591
CGGGGTT32100.08.6829451
CGCCATT27900.08.6354711
AGATCGG73150.08.60610294
CGCCGAT17600.08.58929351
CGCGAAC5050.00334224698.4192091
CTCCGAT22450.08.4171251
Produced by FastQC (version 0.11.5)