FastQCFastQC Report
Wed 25 May 2016
SRR1033283_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033283_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11336834
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA12450.012.85625914-15
GCATACG14750.011.80904514-15
CCCGTAT16100.011.4654681
CGTATCG11000.010.3228463
CTCGCGT11500.010.2895221
CTCGGTT20800.010.2400541
GATCGGA55200.09.69947994
ATACGAG16050.09.20359216-17
TCGCGTC11052.0008883E-108.9896982
CGATAAC14850.08.9245151
CCGGGAT32900.08.9196711
AGATCGG71050.08.85774994
ATAACGT13501.8189894E-128.7616753
CGCGTCG8108.550105E-78.7616753
GTCCGAT11453.9835868E-108.6809431
CTCGTAT13109.094947E-128.6714751
CTGGAAT122400.08.623361
CGGCATA20700.08.58194212-13
TCCGATT20400.08.5794542
CGCCTGT42800.08.4047141
Produced by FastQC (version 0.11.5)