##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033283_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11336834 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16203227461917 34.0 31.0 34.0 30.0 34.0 2 32.30075407296252 34.0 31.0 34.0 31.0 34.0 3 32.35459229622662 34.0 31.0 34.0 31.0 34.0 4 35.75218398716961 37.0 37.0 37.0 35.0 37.0 5 35.73831609424642 37.0 37.0 37.0 35.0 37.0 6 35.6558233101058 37.0 37.0 37.0 35.0 37.0 7 35.639636604011315 37.0 37.0 37.0 35.0 37.0 8 35.62494775878345 37.0 37.0 37.0 35.0 37.0 9 37.37423128891188 39.0 39.0 39.0 35.0 39.0 10-11 37.48772611471598 39.0 39.0 39.0 35.0 39.0 12-13 37.43159739306406 39.0 38.5 39.0 35.0 39.0 14-15 38.90507327707189 41.0 39.0 41.0 36.0 41.0 16-17 39.00062169032377 41.0 39.0 41.0 36.0 41.0 18-19 39.055323779107994 41.0 39.5 41.0 36.0 41.0 20-21 39.01847217662356 41.0 39.0 41.0 36.0 41.0 22-23 38.95170468227725 41.0 39.0 41.0 35.5 41.0 24-25 38.905840510675205 41.0 39.0 41.0 35.0 41.0 26-27 38.83103470510373 41.0 39.0 41.0 35.0 41.0 28-29 38.72739421782131 41.0 39.0 41.0 35.0 41.0 30-31 38.72821322954892 41.0 39.0 41.0 35.0 41.0 32-33 38.822765332896296 41.0 39.0 41.0 35.0 41.0 34-35 38.74909692600245 41.0 39.0 41.0 35.0 41.0 36-37 38.68767973492423 41.0 39.0 41.0 35.0 41.0 38-39 38.609470906956915 41.0 39.0 41.0 35.0 41.0 40-41 38.47437044592873 41.0 38.5 41.0 34.0 41.0 42-43 38.34427089608968 41.0 38.0 41.0 34.0 41.0 44-45 38.203108160532295 40.0 38.0 41.0 33.5 41.0 46-47 38.078885516009144 40.0 38.0 41.0 33.0 41.0 48-49 37.94453967483338 40.0 37.5 41.0 33.0 41.0 50-51 37.769712469989415 40.0 37.0 41.0 33.0 41.0 52-53 37.58844753305905 40.0 37.0 41.0 33.0 41.0 54-55 37.386412732161375 40.0 36.0 41.0 32.5 41.0 56-57 37.156188359113315 39.5 35.5 41.0 32.0 41.0 58-59 36.91716465990417 39.0 35.0 41.0 32.0 41.0 60-61 36.658923778896295 39.0 35.0 41.0 31.5 41.0 62-63 36.37196967865984 38.5 35.0 41.0 31.0 41.0 64-65 36.10212992445686 37.5 35.0 40.5 31.0 41.0 66-67 35.79175971880686 37.0 35.0 40.0 31.0 41.0 68-69 35.4736035651576 36.5 35.0 39.5 31.0 41.0 70-71 35.14931271817158 36.0 35.0 39.0 30.5 41.0 72-73 34.79871390901552 35.5 35.0 39.0 30.0 41.0 74-75 34.46378159898963 35.0 34.0 37.5 30.0 40.0 76-77 34.20698067026473 35.0 34.0 37.0 30.0 39.0 78-79 34.037532612720625 35.0 34.0 37.0 30.0 39.0 80-81 33.774911805182995 35.0 34.0 36.0 30.0 39.0 82-83 33.53935441764429 35.0 34.0 36.0 30.0 37.0 84-85 33.319721229048604 35.0 34.0 35.5 30.0 37.0 86-87 33.12293979077404 35.0 34.0 35.0 29.5 36.5 88-89 32.944512727274656 35.0 34.0 35.0 29.0 36.0 90-91 32.81708491100778 35.0 34.0 35.0 29.0 36.0 92-93 32.665575636019724 35.0 34.0 35.0 29.0 36.0 94-95 32.5406941655845 35.0 34.0 35.0 29.0 36.0 96-97 32.46285726685245 35.0 34.0 35.0 29.0 35.0 98-99 32.37126961548524 35.0 34.0 35.0 29.0 35.0 100 32.27721743125109 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03372467461614548 1101 2 -0.09131065069275479 1101 3 -0.03283956702431112 1101 4 -0.044716425673669846 1101 5 -0.02177607271011084 1101 6 -0.011330024944854244 1101 7 0.08105833903525195 1101 8 0.1104706542472158 1101 9 0.13237908458525993 1101 10-11 0.13404354978036537 1101 12-13 0.07956324825025263 1101 14-15 0.19469328316877466 1101 16-17 0.24446164334354847 1101 18-19 0.2008512935882223 1101 20-21 0.22395388135784344 1101 22-23 0.2906460031472733 1101 24-25 0.23761611933805682 1101 26-27 0.10699227467819128 1101 28-29 0.14089392256112632 1101 30-31 0.20662071818550487 1101 32-33 0.1660693234838746 1101 34-35 0.20344326066032892 1101 36-37 0.17063689730655085 1101 38-39 0.19187931333767239 1101 40-41 0.16442234214252238 1101 42-43 0.17242024277906154 1101 44-45 0.09808056158951928 1101 46-47 0.09270676895847885 1101 48-49 0.12243501760929121 1101 50-51 0.12063564368549606 1101 52-53 0.11876425642485344 1101 54-55 0.170581804579804 1101 56-57 0.15268984542580455 1101 58-59 0.15044063848934286 1101 60-61 0.1106702074661996 1101 62-63 0.12874238862261222 1101 64-65 0.12644993825890083 1101 66-67 0.07713713161254532 1101 68-69 0.13336235025960974 1101 70-71 0.12890692907483725 1101 72-73 0.16521437334778 1101 74-75 0.10376318022324682 1101 76-77 0.14055440925026375 1101 78-79 0.1658114864149809 1101 80-81 0.1563970795536065 1101 82-83 0.18666951321054626 1101 84-85 0.20047708888762372 1101 86-87 0.1709784384203914 1101 88-89 0.18544918277282818 1101 90-91 0.18228553450254736 1101 92-93 0.17528392069671384 1101 94-95 0.1797913860316953 1101 96-97 0.18289083244116 1101 98-99 0.141211219524898 1101 100 0.16950378407365463 1102 1 0.05805496694086543 1102 2 0.054802295041199045 1102 3 0.037757867096551934 1102 4 0.007759619218923319 1102 5 0.06578774203482851 1102 6 0.05252686097357184 1102 7 0.15428036325192807 1102 8 0.1571317985770193 1102 9 0.17957350090734536 1102 10-11 0.22798366744974885 1102 12-13 0.13777925288223258 1102 14-15 0.290861465369467 1102 16-17 0.24289200758996543 1102 18-19 0.2651147098424431 1102 20-21 0.22748879867329208 1102 22-23 0.26461188985917516 1102 24-25 0.2876888448348396 1102 26-27 0.27242763189708086 1102 28-29 0.29945442358086183 1102 30-31 0.33574289680179703 1102 32-33 0.2954077278456708 1102 34-35 0.323343620473203 1102 36-37 0.30147797116312347 1102 38-39 0.29692426870670374 1102 40-41 0.27874759936599247 1102 42-43 0.29095011920042424 1102 44-45 0.2568680484502579 1102 46-47 0.25014173648246185 1102 48-49 0.2729300619009365 1102 50-51 0.24331007138265903 1102 52-53 0.2637193403501499 1102 54-55 0.24073651392716044 1102 56-57 0.2667673475754455 1102 58-59 0.25003044155658927 1102 60-61 0.2962922569919044 1102 62-63 0.30560208568311253 1102 64-65 0.24225044408861152 1102 66-67 0.23903122548279043 1102 68-69 0.16503687766830666 1102 70-71 0.1553584746904093 1102 72-73 0.18446270630139594 1102 74-75 0.14754180433671138 1102 76-77 0.22448926663226132 1102 78-79 0.24754257407250435 1102 80-81 0.23614071928495406 1102 82-83 0.2637016176069409 1102 84-85 0.2935998082287341 1102 86-87 0.2641060084273903 1102 88-89 0.2747557640593925 1102 90-91 0.24666858014074222 1102 92-93 0.2693638715812625 1102 94-95 0.3560701601839895 1102 96-97 0.33140976303585035 1102 98-99 0.31980984974749305 1102 100 0.3366328314074991 1103 1 -0.024832236277084974 1103 2 -0.03640976671687213 1103 3 -0.031106988737874985 1103 4 -0.03195695389149478 1103 5 -0.0038544314076176533 1103 6 0.0858987780865732 1103 7 0.09764910489376177 1103 8 0.13812117514275002 1103 9 0.16624614150091332 1103 10-11 0.1605771815071364 1103 12-13 0.07433486752061214 1103 14-15 0.24776231661233084 1103 16-17 0.21598188849149835 1103 18-19 0.24164118682127622 1103 20-21 0.2292305951242497 1103 22-23 0.2198585631543395 1103 24-25 0.22339833283060528 1103 26-27 0.24319317421598186 1103 28-29 0.221824863369946 1103 30-31 0.17846913731000313 1103 32-33 0.19850802392257094 1103 34-35 0.23232452098184808 1103 36-37 0.2023812374525562 1103 38-39 0.21788676565780207 1103 40-41 0.23358086499101915 1103 42-43 0.2307492705869194 1103 44-45 0.19477263530978206 1103 46-47 0.15416118529134337 1103 48-49 0.2750640816204495 1103 50-51 0.20814817352759718 1103 52-53 0.23050858205438374 1103 54-55 0.18554664572305057 1103 56-57 0.222486260791932 1103 58-59 0.22890176621263691 1103 60-61 0.186813675454502 1103 62-63 0.1705845515327553 1103 64-65 0.13541003096460713 1103 66-67 0.14973038375948278 1103 68-69 0.16413884118767186 1103 70-71 0.13442231347365663 1103 72-73 0.13212348040016764 1103 74-75 0.1324535808657501 1103 76-77 0.1695168514161054 1103 78-79 0.1757367500084115 1103 80-81 0.15647705381481813 1103 82-83 0.1719009669314886 1103 84-85 0.17062935671449964 1103 86-87 0.1880884225737134 1103 88-89 0.22121876036182186 1103 90-91 0.18942479560658398 1103 92-93 0.24558694158707084 1103 94-95 0.22615526646256257 1103 96-97 0.2170992958126945 1103 98-99 0.19445693574899536 1103 100 0.1812689289454923 1104 1 0.0314595467852854 1104 2 0.03649133987571673 1104 3 0.024129304155280806 1104 4 0.024453740723373585 1104 5 0.048441254178918314 1104 6 0.11238625923589751 1104 7 0.1336051317683129 1104 8 0.10539609636839486 1104 9 0.12623476683018708 1104 10-11 0.16997973242702358 1104 12-13 0.10241489068086196 1104 14-15 0.22200012836982808 1104 16-17 0.22897576506018424 1104 18-19 0.23599001367085748 1104 20-21 0.23200762990238388 1104 22-23 0.23054394514652188 1104 24-25 0.20966121559702344 1104 26-27 0.24164050250264069 1104 28-29 0.19223460106606183 1104 30-31 0.20398338967112295 1104 32-33 0.20936971868449916 1104 34-35 0.24389539419130557 1104 36-37 0.2461726035777403 1104 38-39 0.2583263854920048 1104 40-41 0.2318722739814234 1104 42-43 0.2309909993295136 1104 44-45 0.25068942974828445 1104 46-47 0.20517177781517404 1104 48-49 0.14911779027365668 1104 50-51 0.19460046095069572 1104 52-53 0.2050609053066239 1104 54-55 0.19447975757177005 1104 56-57 0.17607276968522712 1104 58-59 0.1973799438605397 1104 60-61 0.14387103974500803 1104 62-63 0.15037247189384573 1104 64-65 0.13118566408309107 1104 66-67 0.11010002507750016 1104 68-69 0.11449707883407001 1104 70-71 0.11418960527229416 1104 72-73 0.13906023457105476 1104 74-75 0.16812004530849833 1104 76-77 0.1328346228875006 1104 78-79 0.16798043423283815 1104 80-81 0.18278687882889955 1104 82-83 0.2054721842163616 1104 84-85 0.20219732762232923 1104 86-87 0.20819367897401975 1104 88-89 0.208702823602124 1104 90-91 0.23794194570979954 1104 92-93 0.27586441831269326 1104 94-95 0.3168567560391651 1104 96-97 0.21673469258940514 1104 98-99 0.21721378622354592 1104 100 0.23870762610096108 1105 1 -0.09648630924456114 1105 2 -0.12996136644284206 1105 3 -0.0834364869334081 1105 4 -0.04630716940761914 1105 5 -0.011754001350254839 1105 6 0.051373714000547466 1105 7 0.06519952270944884 1105 8 0.05410915814648831 1105 9 0.05651732032650614 1105 10-11 0.12523716199183355 1105 12-13 0.022192494885416636 1105 14-15 0.14025812087687228 1105 16-17 0.1750821467162922 1105 18-19 0.17069483608346303 1105 20-21 0.1664780982457046 1105 22-23 0.17545399335198653 1105 24-25 0.17665168923666386 1105 26-27 0.19461269475741716 1105 28-29 0.21451505491548062 1105 30-31 0.21660910091159025 1105 32-33 0.13258434092215765 1105 34-35 0.1816295528207732 1105 36-37 0.11285374978644569 1105 38-39 0.12521447509809036 1105 40-41 0.15487539067074607 1105 42-43 0.12069304253112279 1105 44-45 0.1860638460063413 1105 46-47 0.10117293542321448 1105 48-49 0.07224230247661723 1105 50-51 0.10404658712181458 1105 52-53 0.1339358992734816 1105 54-55 0.14205308602873856 1105 56-57 0.12213281227486306 1105 58-59 0.11417640145606356 1105 60-61 0.13148298333537412 1105 62-63 0.09885898208930399 1105 64-65 0.09629117628688277 1105 66-67 0.12466543223550275 1105 68-69 0.1411124258343719 1105 70-71 0.1603060969268384 1105 72-73 0.11659226423198277 1105 74-75 0.17641872253397395 1105 76-77 0.1875835614509569 1105 78-79 0.16045841788901072 1105 80-81 0.18772521463628777 1105 82-83 0.130651628191373 1105 84-85 0.1653523541194346 1105 86-87 0.13768356629417866 1105 88-89 0.16635881158765642 1105 90-91 0.20720497856279962 1105 92-93 0.22514778672699975 1105 94-95 0.2450464907614176 1105 96-97 0.20114932745606495 1105 98-99 0.13569495779599805 1105 100 0.17056798137922158 1106 1 -0.11485993970244834 1106 2 -0.09317998882587375 1106 3 -0.08738876629490733 1106 4 -0.10180888553925627 1106 5 -0.09523860237626991 1106 6 -0.04548682059068199 1106 7 -0.007234663937708774 1106 8 0.009341103947001272 1106 9 0.018823071499340926 1106 10-11 0.056222008755760555 1106 12-13 -0.010815792125704604 1106 14-15 0.059053727019410474 1106 16-17 0.07799306348746171 1106 18-19 0.026569603233973282 1106 20-21 0.01787581441526953 1106 22-23 0.06992924968546532 1106 24-25 0.028986316117361355 1106 26-27 0.07782599334989015 1106 28-29 0.048542352805526434 1106 30-31 0.0966816461385136 1106 32-33 0.048718536535474755 1106 34-35 0.08679768107069208 1106 36-37 0.051542283553104085 1106 38-39 0.12695611464866374 1106 40-41 0.11021719847659739 1106 42-43 0.10347917115171867 1106 44-45 0.08624736923191989 1106 46-47 0.07482942843438423 1106 48-49 0.03926719096751441 1106 50-51 0.07523804762870867 1106 52-53 0.06660572610486781 1106 54-55 0.08606856302317567 1106 56-57 0.054077512364578695 1106 58-59 0.06617528914262039 1106 60-61 0.049879266263815225 1106 62-63 0.0893609271517235 1106 64-65 0.027928730530561552 1106 66-67 0.03846377316317984 1106 68-69 0.07512095989257972 1106 70-71 0.06674796135325067 1106 72-73 0.06487453680199451 1106 74-75 0.07438731747716076 1106 76-77 0.07474201318254359 1106 78-79 0.07362743270610395 1106 80-81 0.009941078456513708 1106 82-83 0.06373487499453745 1106 84-85 0.08464843054311189 1106 86-87 0.08605099969850016 1106 88-89 0.08474721816504172 1106 90-91 0.06794387724227846 1106 92-93 0.051241844087357435 1106 94-95 0.003313959612853523 1106 96-97 0.04805860271925155 1106 98-99 0.025681589229257895 1106 100 -0.015111871204929628 1107 1 -0.12685822957971027 1107 2 -0.10724015197280323 1107 3 -0.03682423123247247 1107 4 0.015323681773388387 1107 5 -0.004819449386353369 1107 6 0.0631493232664937 1107 7 0.0808658809720626 1107 8 0.10630270667432029 1107 9 0.06819145437399499 1107 10-11 0.118067652325891 1107 12-13 0.014950195006221634 1107 14-15 0.14592628586719059 1107 16-17 3.608088430837597E-4 1107 18-19 0.07301507541914276 1107 20-21 0.0630096207794395 1107 22-23 0.03397045176541269 1107 24-25 0.04370482181616353 1107 26-27 0.08394675320558065 1107 28-29 0.03538041585608909 1107 30-31 0.043838577109788446 1107 32-33 0.048738313110675335 1107 34-35 0.08924919494949535 1107 36-37 0.05080109278006262 1107 38-39 0.07738040333664742 1107 40-41 0.07380136029714635 1107 42-43 0.05451683422656828 1107 44-45 0.09553517628803831 1107 46-47 0.12556379759138991 1107 48-49 0.11015022847474398 1107 50-51 0.14625664584462328 1107 52-53 0.1395641919507682 1107 54-55 0.14978613858176004 1107 56-57 0.1735058405032035 1107 58-59 0.22262834154679467 1107 60-61 0.187152031260851 1107 62-63 0.15013041044952757 1107 64-65 0.10041656097394025 1107 66-67 0.1399658381121327 1107 68-69 0.11003345275512544 1107 70-71 0.15858292777685534 1107 72-73 0.1900204241088943 1107 74-75 0.15715642732429558 1107 76-77 0.17937693710354097 1107 78-79 0.13599854308506565 1107 80-81 0.12341621163732697 1107 82-83 0.2125225058147251 1107 84-85 0.17729562411584254 1107 86-87 0.13364455517186258 1107 88-89 0.1740226108617975 1107 90-91 0.1535442082063767 1107 92-93 0.20514394370843547 1107 94-95 0.21748773688000256 1107 96-97 0.17651976636452105 1107 98-99 0.1411767282492704 1107 100 0.1791730432014731 1108 1 -0.10230325272716101 1108 2 -0.12531620916164599 1108 3 -0.10015895687031673 1108 4 -0.03685770081926876 1108 5 -0.07304634792974696 1108 6 0.008156345297066991 1108 7 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48-49 -0.11446187237372385 2214 50-51 -0.11841762601338957 2214 52-53 -0.058716695861001256 2214 54-55 -0.07499929176348985 2214 56-57 -0.039824544559550645 2214 58-59 -0.07467465766732317 2214 60-61 -0.07423442130900071 2214 62-63 -0.07093327992799914 2214 64-65 -0.04491701407761184 2214 66-67 -0.07366932010586424 2214 68-69 -0.054939192285260674 2214 70-71 -0.09141729482217897 2214 72-73 -0.09219772683655947 2214 74-75 -0.07794620218417947 2214 76-77 -0.1139396468760907 2214 78-79 -0.08628211555992493 2214 80-81 -0.1281480462016944 2214 82-83 -0.15312921316476036 2214 84-85 -0.09206383929260653 2214 86-87 -0.09489488985440886 2214 88-89 -0.0713181600794286 2214 90-91 -0.06943234370337592 2214 92-93 -0.05797113569123269 2214 94-95 -0.13075714671831662 2214 96-97 -0.1439606870478798 2214 98-99 -0.17899384869230772 2214 100 -0.17072590155912337 2215 1 -0.13882208662471385 2215 2 -0.22996708541192845 2215 3 -0.22479985825022197 2215 4 -0.17128263862688442 2215 5 -0.11356768441181941 2215 6 -0.16398767178770157 2215 7 -0.24969030970365225 2215 8 -0.2894194434017905 2215 9 -0.40905292524465864 2215 10-11 -0.4202017654672403 2215 12-13 -0.3299920710497304 2215 14-15 -0.500443171274263 2215 16-17 -0.2969903051314944 2215 18-19 -0.3537776075960579 2215 20-21 -0.36582048710903337 2215 22-23 -0.357485556461107 2215 24-25 -0.3410405569261954 2215 26-27 -0.3000868032900925 2215 28-29 -0.3306645838781179 2215 30-31 -0.30885280616188027 2215 32-33 -0.32596154965821 2215 34-35 -0.3325819411954001 2215 36-37 -0.3447714759441425 2215 38-39 -0.29766128532192937 2215 40-41 -0.33352421409023236 2215 42-43 -0.2883535818399494 2215 44-45 -0.32184502602598997 2215 46-47 -0.2828245270666372 2215 48-49 -0.3520175212869887 2215 50-51 -0.27647011281781886 2215 52-53 -0.2900852749420437 2215 54-55 -0.2911112588028715 2215 56-57 -0.2780276904141985 2215 58-59 -0.318527719444603 2215 60-61 -0.33354402259377025 2215 62-63 -0.2594616051857983 2215 64-65 -0.23458383757582624 2215 66-67 -0.26070287516991186 2215 68-69 -0.31746148888350234 2215 70-71 -0.29070633300278104 2215 72-73 -0.2923092992610421 2215 74-75 -0.2795732530152293 2215 76-77 -0.265006361683902 2215 78-79 -0.2743130910155003 2215 80-81 -0.2566269377812489 2215 82-83 -0.30244369070462085 2215 84-85 -0.2987285785671361 2215 86-87 -0.28426262831300164 2215 88-89 -0.30089500009483316 2215 90-91 -0.29500191780648066 2215 92-93 -0.3232633950822077 2215 94-95 -0.25756310693496687 2215 96-97 -0.3338001293711699 2215 98-99 -0.4329075749523099 2215 100 -0.4425994426000308 2216 1 -0.09368107583355822 2216 2 -0.12528615992206227 2216 3 -0.17684677591550724 2216 4 -0.1659996237327448 2216 5 -0.08392493713067495 2216 6 -0.08997810966777564 2216 7 -0.17082705401289644 2216 8 -0.3329805719741401 2216 9 -0.44935254495102583 2216 10-11 -0.30284695049696353 2216 12-13 -0.22822587282708184 2216 14-15 -0.40749261559003713 2216 16-17 -0.4697400336161337 2216 18-19 -0.4301170479352123 2216 20-21 -0.3956188296182148 2216 22-23 -0.38242867813610104 2216 24-25 -0.36333240848173176 2216 26-27 -0.4344249892819434 2216 28-29 -0.4190828294538278 2216 30-31 -0.40181973140914096 2216 32-33 -0.3906483081127874 2216 34-35 -0.4242237418770358 2216 36-37 -0.4364411808242181 2216 38-39 -0.3545090059935987 2216 40-41 -0.6707353225229582 2216 42-43 -0.871350270327973 2216 44-45 -0.5531370455973743 2216 46-47 -0.4157095008810714 2216 48-49 -0.5434020059383755 2216 50-51 -0.4833313949130442 2216 52-53 -0.4334309817524371 2216 54-55 -0.5693929950485028 2216 56-57 -0.4421984093633782 2216 58-59 -0.5830803538000708 2216 60-61 -0.6503234187344304 2216 62-63 -0.5731963047914945 2216 64-65 -0.5613951179125962 2216 66-67 -0.5208543162416106 2216 68-69 -0.594454257046813 2216 70-71 -0.5778584654438177 2216 72-73 -0.6374041495720206 2216 74-75 -0.5439424048200863 2216 76-77 -0.5357137661064115 2216 78-79 -0.5976688578505502 2216 80-81 -0.5448420623934496 2216 82-83 -0.7252599268675226 2216 84-85 -0.5342782371048074 2216 86-87 -0.4367082764697301 2216 88-89 -0.519761053124526 2216 90-91 -0.7080410527402137 2216 92-93 -1.0325901949460388 2216 94-95 -0.9059083989372603 2216 96-97 -0.8956474774243333 2216 98-99 -0.7280768780861706 2216 100 -0.6439032496068648 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63506.0 3 11316.0 4 8159.0 5 7508.0 6 9175.0 7 11758.0 8 12486.0 9 12389.0 10 14458.0 11 13938.0 12 14366.0 13 15228.0 14 16106.0 15 16666.0 16 17270.0 17 18072.0 18 18115.0 19 16988.0 20 17671.0 21 18629.0 22 20444.0 23 25008.0 24 33999.0 25 49914.0 26 67117.0 27 73237.0 28 82339.0 29 91139.0 30 113077.0 31 143011.0 32 186619.0 33 250415.0 34 360426.0 35 592970.0 36 1240834.0 37 2659374.0 38 3648390.0 39 1360321.0 40 4396.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.315179465035314 12.436921307757762 11.516164904325192 49.73173432288173 2 20.722933357947696 19.980086840103922 36.438901366017234 22.85807843593115 3 25.22558578891986 22.67841607797255 23.924825661542762 28.171172471564827 4 27.06873194630481 29.604119471700663 18.740443725840308 24.58670485615422 5 28.1354314547438 31.267921365046863 21.167265127984006 19.429382052225336 6 22.54757271409354 33.75161445460287 22.423791567902516 21.27702126340107 7 20.04059507550398 17.84674300814916 39.36605452448976 22.7466073918571 8 20.841897675934796 21.668905562202994 29.260153789391865 28.229042972470346 9 21.401485316731165 20.86136124189467 31.37041409838733 26.366739342986843 10-11 25.06912510541656 30.047483054542468 21.500112633233957 23.383279206807014 12-13 23.327478274978912 24.191526181066962 27.330774197362302 25.150221346591824 14-15 23.774598991767945 25.364243034304113 26.593305500054093 24.267852473873848 16-17 24.61032650425661 25.504296842393938 25.503935073623474 24.381441579725983 18-19 24.37435263353694 25.62912497996017 25.373259070738452 24.623263315764436 20-21 24.27298259610418 25.77789859680668 25.675774459754315 24.273344347334827 22-23 24.455271198290458 25.838902642483784 25.641837041981912 24.063989117243846 24-25 24.209214847813772 25.96994010849943 25.674513713440632 24.146331330246166 26-27 24.021916611900863 26.027452000899775 25.7588863944108 24.191744992788557 28-29 24.18550029397793 25.72999373229351 25.659033238251798 24.425472735476763 30-31 24.106117606762183 25.814076131764107 25.676657375305723 24.403148886167987 32-33 24.011862571155227 26.02513629466569 25.80256533702443 24.160435797154655 34-35 24.364030529552437 25.87727636662518 25.447722074812663 24.31097102900973 36-37 24.20518028874196 25.748913902119032 25.57430114431316 24.471604664825847 38-39 24.115037075332722 25.912051949882724 25.73472833322372 24.238182641560833 40-41 24.366597073667595 25.768904173650363 25.5927514482572 24.27174730442484 42-43 24.1210500083217 25.65528396852677 25.83048990293414 24.393176120217397 44-45 24.15766122557682 25.846355218362582 25.87864566216551 24.117337893895087 46-47 24.242085432495827 25.826991079553085 25.580498834296634 24.35042465365446 48-49 24.142416181235753 25.692002770958066 25.74743872166916 24.418142326137026 50-51 24.168764942490174 25.83165000406118 25.83631594560139 24.163269107847256 52-53 24.249723452877575 25.868903930643487 25.578416352787574 24.30295626369136 54-55 24.05140762657326 25.743444775165596 25.73066555200989 24.47448204625125 56-57 24.04842560206474 25.796905239683127 25.946440976199376 24.208228182052764 58-59 24.250157331724587 25.79194863315098 25.6592447591594 24.29864927596503 60-61 24.087965968806273 25.84835758579053 25.760851603013336 24.302824842389867 62-63 23.9708154918971 26.02035434444052 25.917047172068308 24.091782991594073 64-65 24.13992059033412 25.883914666162983 25.70246927255468 24.273695470948216 66-67 24.10894218528607 25.808908384494288 25.7676482902705 24.314501139949137 68-69 24.1062047209896 25.96495727080465 25.852956306823938 24.075881701381814 70-71 24.21774743274396 25.84606390840604 25.66627325972579 24.26991539912421 72-73 24.10359609375107 25.888739514536148 25.738386791417277 24.269277600295506 74-75 24.072128296930607 26.044411407225155 25.776573109273475 24.10688718657076 76-77 24.267383005247083 25.861364453597464 25.672508342411255 24.198744198744198 78-79 24.111537051620722 25.712917234887065 25.881240453267985 24.294305260224224 80-81 24.083809435631427 25.95306413279839 25.91263241409961 24.050494017470584 82-83 24.18668768604933 25.852079052623118 25.722835986606547 24.23839727472101 84-85 24.136757865591896 25.774661026075517 25.871136681814566 24.217444426518025 86-87 24.10422240797503 26.011536274500617 25.90219390012371 23.98204741740064 88-89 24.21848055968723 25.94356528046949 25.673738347925873 24.164215811917416 90-91 24.111594224586096 25.882638519101008 25.75117244370061 24.254594812612286 92-93 24.037867481224062 26.073066537037825 25.893716395873373 23.99534958586474 94-95 24.349505223159642 25.975006875948697 25.617192574434682 24.05829532645697 96-97 24.214278159161807 25.900422759991915 25.749050771581388 24.13624830926489 98-99 24.216878719733558 26.040853705987104 25.798240940345195 23.944026633934136 100 24.43805429954209 25.80037153945895 25.465579586093558 24.295994574905404 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 31.0 1 27.0 2 27.0 3 49.0 4 77.0 5 111.0 6 182.5 7 260.5 8 352.5 9 453.0 10 555.0 11 674.0 12 788.5 13 976.5 14 1184.5 15 1339.5 16 1555.5 17 1997.5 18 2540.5 19 3310.0 20 4863.5 21 7367.5 22 11094.0 23 16722.5 24 24071.5 25 33282.0 26 45523.0 27 59684.0 28 74206.0 29 88846.0 30 104880.5 31 121962.5 32 138766.0 33 156455.5 34 174608.0 35 195535.5 36 219320.0 37 243117.5 38 263103.5 39 281314.0 40 297693.5 41 311902.0 42 328075.5 43 344694.5 44 354252.5 45 364324.0 46 377359.5 47 381510.0 48 383541.5 49 382186.5 50 382641.0 51 384839.0 52 378963.0 53 370624.5 54 361538.5 55 351757.0 56 334166.5 57 312005.0 58 297953.0 59 287936.0 60 276794.5 61 260465.0 62 238358.5 63 218370.0 64 199720.0 65 177419.5 66 151032.0 67 127772.5 68 108686.5 69 87756.0 70 66317.5 71 48397.0 72 35339.0 73 25202.0 74 17429.5 75 11351.0 76 7036.5 77 4280.0 78 2464.0 79 1226.0 80 683.5 81 420.0 82 263.5 83 183.5 84 136.0 85 104.0 86 84.0 87 59.5 88 44.5 89 37.0 90 29.5 91 28.0 92 23.0 93 19.5 94 22.0 95 14.5 96 12.5 97 12.5 98 9.5 99 15.5 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6817335421864693 2 0.519236675777382 3 0.5133531989618971 4 0.5022654473021304 5 0.5181781791988839 6 0.7187632808242583 7 0.8038840473451406 8 0.8548859408191035 9 0.8883608951141033 10-11 0.38407548350800585 12-13 0.6244424148752642 14-15 0.4440834187040226 16-17 0.03200187988992341 18-19 3.219593759598138E-4 20-21 0.027154847640884574 22-23 0.0 24-25 0.0 26-27 0.006033430497438702 28-29 0.00837094377495516 30-31 0.0028006055306093393 32-33 0.0 34-35 0.03769570940176067 36-37 2.9549696149736335E-4 38-39 0.009372105122117868 40-41 0.11588332333348093 42-43 0.09719644832058051 44-45 0.07413886451896536 46-47 0.0426441809062389 48-49 0.17792004363828562 50-51 0.08886078776490862 52-53 0.07316416558626508 54-55 0.12143601996818512 56-57 0.08033106950317875 58-59 0.09915907739321225 60-61 0.13011569191186886 62-63 0.1559341876223997 64-65 0.061560396844480565 66-67 0.07671453952664387 68-69 0.0776804176545233 70-71 0.03712676749081798 72-73 0.07473426884437048 74-75 0.17910203148427506 76-77 0.16022109876531668 78-79 0.12851471583689061 80-81 0.15657369597190893 82-83 0.10627746688361142 84-85 0.19460900635927103 86-87 0.19331675845302138 88-89 0.11223151013766278 90-91 0.08783316400328346 92-93 0.18014288645313145 94-95 0.13019507915525622 96-97 0.17651312526936533 98-99 0.194900092918358 100 0.1681157190799477 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1336834E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.76192778090724 #Duplication Level Percentage of deduplicated Percentage of total 1 79.95533161899183 46.98329422293687 2 10.850695183817678 12.752155335282648 3 3.648681901791335 6.4321074722569715 4 1.6532785309471516 3.885993345489638 5 0.9418209016897501 2.767160590382102 6 0.5860612385562217 2.066285290513785 7 0.4079281136253013 1.6779449646856175 8 0.3068067937969384 1.4422846927829918 9 0.2303709159160028 1.2183335211489852 >10 1.2968077392587372 14.113910079062258 >50 0.08988925162629224 3.6146904493409293 >100 0.032056539239242156 2.946325558798615 >500 2.7127074356820093E-4 0.09951447731858855 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.006633245225254246 0.0 0.0 0.0 0.0 2 0.006659707639716697 6.174563374571771E-5 0.0 2.6462414462450452E-5 0.0 3 0.006730274078283231 6.174563374571771E-5 0.0 2.6462414462450452E-5 0.0 4 0.006915510979520385 6.174563374571771E-5 0.0 3.5283219283267265E-5 0.0 5 0.00703900224701182 6.174563374571771E-5 0.0 3.5283219283267265E-5 0.0 6 0.0071801351241448895 6.174563374571771E-5 0.0 3.5283219283267265E-5 0.0 7 0.007224239148248973 7.056643856653453E-5 0.0 3.5283219283267265E-5 0.0 8 0.007321268001277958 7.938724338735136E-5 0.0 3.5283219283267265E-5 0.0 9 0.007418296854306943 8.820804820816818E-5 0.0 3.5283219283267265E-5 0.0 10-11 0.00778877065678125 1.0584965784980181E-4 0.0 4.410402410408409E-5 0.0 12-13 0.008168065264076374 1.1026006026021022E-4 0.0 4.410402410408409E-5 0.0 14-15 0.008789932003943958 1.2790166990184383E-4 0.0 4.8514426514492497E-5 0.0 16-17 0.009495596389609303 1.455432795434775E-4 0.0 6.174563374571771E-5 0.0 18-19 0.010346804054818127 1.5877448677470272E-4 0.0 7.497684097694295E-5 0.0 20-21 0.011228884536899807 1.6318488918511112E-4 0.0 7.938724338735136E-5 0.0 22-23 0.012155069043085574 1.6759529159551952E-4 0.0 7.938724338735136E-5 0.0 24-25 0.013275311255329311 1.7641609641633635E-4 0.0 7.938724338735136E-5 0.0 26-27 0.014267651797671201 1.7641609641633635E-4 0.0 7.938724338735136E-5 0.0 28-29 0.015198246706267377 1.8523690123715316E-4 0.0 7.938724338735136E-5 0.0 30-31 0.016221460065482127 1.8964730364756156E-4 0.0 8.379764579775976E-5 0.0 32-33 0.01740344791147158 1.9405770605796999E-4 0.0 8.820804820816818E-5 0.0 34-35 0.018717747829773285 2.2052012052042042E-4 0.0 8.820804820816818E-5 0.0 36-37 0.020001174931202133 2.5580333980368766E-4 0.0 9.702885302898499E-5 0.0 38-39 0.02131988525191425 2.6903454703491295E-4 0.0 9.702885302898499E-5 0.0 40-41 0.02275767643770739 2.8667615667654655E-4 0.0 9.702885302898499E-5 0.0 42-43 0.024252802854835838 2.999073639077718E-4 0.0 1.1467046267061863E-4 0.0 44-45 0.025792033296068374 3.5283219283267265E-4 0.0 1.4113287713306906E-4 0.0 46-47 0.027370957358994585 3.6606340006389794E-4 0.0 1.4113287713306906E-4 0.0 48-49 0.028941060617099976 3.704738024743063E-4 0.0 1.4113287713306906E-4 0.0 50-51 0.030449418241459653 3.792946072951231E-4 0.0 1.4113287713306906E-4 0.0 52-53 0.032209168803212604 3.792946072951231E-4 0.0 1.4113287713306906E-4 0.0 54-55 0.03422913310717966 3.969362169367568E-4 0.0 1.4113287713306906E-4 4.410402410408408E-6 56-57 0.036297611837661205 4.10167424167982E-4 0.0 1.499536819538859E-4 8.820804820816816E-6 58-59 0.038767437187489914 4.278090338096156E-4 0.0 1.5877448677470272E-4 8.820804820816816E-6 60-61 0.04132547058552678 4.4104024104084084E-4 0.0 1.8082649882674476E-4 1.3231207231225224E-5 62-63 0.0439496600197198 4.6309225309288293E-4 0.0 1.9405770605796999E-4 1.7641609641633633E-5 64-65 0.04708986653593058 4.763234603241081E-4 0.0 1.9405770605796999E-4 1.7641609641633633E-5 66-67 0.050388847538916065 4.8514426514492497E-4 0.0 1.9405770605796999E-4 1.7641609641633633E-5 68-69 0.054080354356427905 4.895546675553334E-4 0.0 1.9405770605796999E-4 1.7641609641633633E-5 70-71 0.058415779925859376 5.248378868386006E-4 0.0 1.984681084683784E-4 1.7641609641633633E-5 72-73 0.06373031483040151 5.424794964802343E-4 0.0 2.028785108787868E-4 1.7641609641633633E-5 74-75 0.07025329999539554 5.645315085322762E-4 0.0 2.028785108787868E-4 1.7641609641633633E-5 76-77 0.07744666632677166 5.645315085322762E-4 0.0 2.028785108787868E-4 1.7641609641633633E-5 78-79 0.08643947684159439 5.865835205843184E-4 0.0 2.028785108787868E-4 1.7641609641633633E-5 80-81 0.0989782508943855 5.954043254051351E-4 0.0 2.028785108787868E-4 1.7641609641633633E-5 82-83 0.11629790116005932 6.04225130225952E-4 0.0 2.0728891328919522E-4 1.7641609641633633E-5 84-85 0.13960246749665733 6.130459350467687E-4 0.0 2.1169931569960362E-4 1.7641609641633633E-5 86-87 0.16995485688508805 6.174563374571772E-4 0.0 2.3375132775164565E-4 1.7641609641633633E-5 88 0.197427253499522 6.174563374571772E-4 0.0 2.4698253498287086E-4 1.7641609641633633E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATACGA 1245 0.0 12.856259 14-15 GCATACG 1475 0.0 11.809045 14-15 CCCGTAT 1610 0.0 11.465468 1 CGTATCG 1100 0.0 10.322846 3 CTCGCGT 1150 0.0 10.289522 1 CTCGGTT 2080 0.0 10.240054 1 GATCGGA 5520 0.0 9.699479 94 ATACGAG 1605 0.0 9.203592 16-17 TCGCGTC 1105 2.0008883E-10 8.989698 2 CGATAAC 1485 0.0 8.924515 1 CCGGGAT 3290 0.0 8.919671 1 AGATCGG 7105 0.0 8.857749 94 ATAACGT 1350 1.8189894E-12 8.761675 3 CGCGTCG 810 8.550105E-7 8.761675 3 GTCCGAT 1145 3.9835868E-10 8.680943 1 CTCGTAT 1310 9.094947E-12 8.671475 1 CTGGAAT 12240 0.0 8.62336 1 CGGCATA 2070 0.0 8.581942 12-13 TCCGATT 2040 0.0 8.579454 2 CGCCTGT 4280 0.0 8.404714 1 >>END_MODULE