FastQCFastQC Report
Wed 25 May 2016
SRR1033283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11336834
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG18600.015.79448342-43
CGTATGC20950.015.36609344-45
CTCGTAT23100.014.0402742-43
TATGCCG23950.013.34150146-47
ATTCGCG3902.4778821E-512.0466656
CGCGAGT8601.8189894E-1211.5343611
CTCGCAT25750.010.4561141
CCCGTAT14500.010.4244861
CGACCGT7854.971298E-910.2294431
CTCGCGT11700.010.0931511
GATCGGA58550.010.0038571
TCTCGTA30850.09.90455240-41
CCCGTTT23600.09.8074651
ATCTCGT35200.09.54860840-41
TCGCATA6001.8263845E-59.4439242
CGTATCG10609.094947E-119.350813
ATTAGCG4600.00150586069.1921296
CCCGGTT24200.09.1739241
AGATCGG71900.09.1478594
CCGGGAT29150.09.0744891
Produced by FastQC (version 0.11.5)