FastQCFastQC Report
Wed 25 May 2016
SRR1033282_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033282_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10696277
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT3802.421653E-411.2123821
GTCCGAT10450.010.8726131
CTCGTAT13100.010.4801061
GATCGGA52800.09.8729294
CTCGACT16400.09.8146331
CGTATCG9256.9485395E-109.7210573
CGGGGTT32850.09.5114671
CGCGAGT8551.9395884E-89.4128641
CTCGCGA4550.00127141399.3641861
CTCCGGT26100.09.2505731
CGACGAT10304.965841E-109.192461
CGCGGTT12753.6379788E-128.9112781
CGCATAT8007.087692E-78.876471
CCCGTAT15500.08.857381
CTCGGTT22450.08.85671
CGCCATT29400.08.856341
CTGGAAT113300.08.7328371
CTCGCAT24950.08.7282311
CTCAGAT84600.08.617661
CGCGTTT7301.9236277E-58.4306191
Produced by FastQC (version 0.11.5)