##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033282_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10696277 Sequences flagged as poor quality 0 Sequence length 100 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15873074341661 34.0 31.0 34.0 31.0 34.0 2 32.378887158587986 34.0 31.0 34.0 31.0 34.0 3 32.4577592745588 34.0 31.0 34.0 31.0 34.0 4 35.81313984295657 37.0 37.0 37.0 35.0 37.0 5 35.7923786005168 37.0 37.0 37.0 35.0 37.0 6 35.728794140241504 37.0 37.0 37.0 35.0 37.0 7 35.69299701195098 37.0 37.0 37.0 35.0 37.0 8 35.67858377265286 37.0 37.0 37.0 35.0 37.0 9 37.4394820739964 39.0 39.0 39.0 35.0 39.0 10-11 37.55051444535327 39.0 39.0 39.0 35.0 39.0 12-13 37.49122956520292 39.0 39.0 39.0 35.0 39.0 14-15 38.974642952870425 41.0 40.0 41.0 36.0 41.0 16-17 39.07059685346593 41.0 39.0 41.0 36.0 41.0 18-19 39.12083269720857 41.0 39.5 41.0 36.0 41.0 20-21 39.08167706389803 41.0 39.5 41.0 36.0 41.0 22-23 39.01215334083065 41.0 39.0 41.0 36.0 41.0 24-25 38.96635824782773 41.0 39.0 41.0 35.5 41.0 26-27 38.89265694970315 41.0 39.0 41.0 35.0 41.0 28-29 38.793880151009546 41.0 39.0 41.0 35.0 41.0 30-31 38.79124166287018 41.0 39.0 41.0 35.0 41.0 32-33 38.8786245438483 41.0 39.0 41.0 35.0 41.0 34-35 38.80890234050595 41.0 39.0 41.0 35.0 41.0 36-37 38.74639601236954 41.0 39.0 41.0 35.0 41.0 38-39 38.66082310695581 41.0 39.0 41.0 35.0 41.0 40-41 38.52472238705112 41.0 39.0 41.0 34.5 41.0 42-43 38.39493059127021 41.0 38.0 41.0 34.0 41.0 44-45 38.254703529087735 40.0 38.0 41.0 34.0 41.0 46-47 38.12422551323232 40.0 38.0 41.0 33.0 41.0 48-49 37.97876518156738 40.0 37.5 41.0 33.0 41.0 50-51 37.798376248109506 40.0 37.0 41.0 33.0 41.0 52-53 37.61338912595476 40.0 37.0 41.0 33.0 41.0 54-55 37.40778669998916 40.0 36.0 41.0 33.0 41.0 56-57 37.171887891459804 39.5 35.5 41.0 32.0 41.0 58-59 36.927690541297686 39.0 35.0 41.0 32.0 41.0 60-61 36.65873345464034 39.0 35.0 41.0 32.0 41.0 62-63 36.36590843711321 38.5 35.0 41.0 31.0 41.0 64-65 36.09002221053176 37.5 35.0 40.5 31.0 41.0 66-67 35.77240805375553 37.0 35.0 40.0 31.0 41.0 68-69 35.4527104617803 36.5 35.0 39.5 31.0 41.0 70-71 35.12578988932317 36.0 35.0 39.0 30.5 41.0 72-73 34.77607348799961 35.0 35.0 39.0 30.0 41.0 74-75 34.44195559819552 35.0 34.0 37.5 30.0 40.0 76-77 34.18713670186365 35.0 34.0 37.0 30.0 39.0 78-79 34.02276039597703 35.0 34.0 37.0 30.0 39.0 80-81 33.76566299657348 35.0 34.0 36.0 30.0 38.5 82-83 33.534880500944396 35.0 34.0 36.0 30.0 37.0 84-85 33.31964804202434 35.0 34.0 35.5 30.0 37.0 86-87 33.12662055217905 35.0 34.0 35.0 29.5 36.5 88-89 32.953191844227675 35.0 34.0 35.0 29.0 36.0 90-91 32.82648266307987 35.0 34.0 35.0 29.0 36.0 92-93 32.67935908914849 35.0 34.0 35.0 29.0 36.0 94-95 32.55574710714765 35.0 34.0 35.0 29.0 35.5 96-97 32.47735903810269 35.0 34.0 35.0 29.0 35.0 98-99 32.38530887896789 35.0 34.0 35.0 29.0 35.0 100 32.28951466010089 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04082889694408465 1101 2 -0.0747813695840236 1101 3 -0.02890444265519676 1101 4 -4.874028081687243E-4 1101 5 -0.014534577367406598 1101 6 0.034289687439155614 1101 7 0.1426054683305651 1101 8 0.12520035503705884 1101 9 0.16086190044908477 1101 10-11 0.1640380705325839 1101 12-13 0.12350998650700262 1101 14-15 0.22111450004302924 1101 16-17 0.2675532078972651 1101 18-19 0.20001137371035327 1101 20-21 0.24500453023941304 1101 22-23 0.290259494477084 1101 24-25 0.25988286820562223 1101 26-27 0.1517042791014802 1101 28-29 0.15831021749669816 1101 30-31 0.2555398322984104 1101 32-33 0.18881439078538165 1101 34-35 0.2349855800190923 1101 36-37 0.20160577392117318 1101 38-39 0.22305747142437582 1101 40-41 0.16176292484598065 1101 42-43 0.14108107525335356 1101 44-45 0.0989694841894675 1101 46-47 0.06142268168484577 1101 48-49 0.10998791589462087 1101 50-51 0.11723961636081981 1101 52-53 0.11265976652290988 1101 54-55 0.15189438563652402 1101 56-57 0.1219614228278516 1101 58-59 0.15460968479874282 1101 60-61 0.11086775450593933 1101 62-63 0.1727280622603402 1101 64-65 0.11203440465806835 1101 66-67 0.009865211454474831 1101 68-69 0.12640663316696532 1101 70-71 0.11687493514351388 1101 72-73 0.12466081342928703 1101 74-75 0.08157322855650762 1101 76-77 0.13597462980298047 1101 78-79 0.17957851854065154 1101 80-81 0.15640095222277495 1101 82-83 0.17966397540735102 1101 84-85 0.20079173924523985 1101 86-87 0.19187345977145753 1101 88-89 0.2051435714408285 1101 90-91 0.16404008640355983 1101 92-93 0.18048499493674797 1101 94-95 0.14222499065147076 1101 96-97 0.08429727145547616 1101 98-99 0.07639031012062958 1101 100 0.09475068647333984 1102 1 0.040703261389516854 1102 2 0.0558772677833872 1102 3 0.058801174638524856 1102 4 0.015865598307776452 1102 5 0.03859777938801301 1102 6 0.07696011047952567 1102 7 0.1088753732194121 1102 8 0.1292984555433705 1102 9 0.19423886578744032 1102 10-11 0.23585504562103665 1102 12-13 0.13123666920471777 1102 14-15 0.30683593926252684 1102 16-17 0.30847575558230744 1102 18-19 0.28915702858839154 1102 20-21 0.2676930742902144 1102 22-23 0.31037632483861444 1102 24-25 0.32473648931707544 1102 26-27 0.33074860628715186 1102 28-29 0.33345865573677 1102 30-31 0.3683062070271603 1102 32-33 0.38812687498705145 1102 34-35 0.3970971659602185 1102 36-37 0.4087015940359606 1102 38-39 0.39323232416035125 1102 40-41 0.3636289658437093 1102 42-43 0.3934796795290083 1102 44-45 0.3627107141920476 1102 46-47 0.35865873145010596 1102 48-49 0.36576704251568515 1102 50-51 0.3639195459956639 1102 52-53 0.36323999778469584 1102 54-55 0.3614271534964786 1102 56-57 0.31263265164739096 1102 58-59 0.317895008894169 1102 60-61 0.36077869902912596 1102 62-63 0.35213709091807033 1102 64-65 0.3087195292792515 1102 66-67 0.31407380890929915 1102 68-69 0.2632447007719634 1102 70-71 0.28886157569939996 1102 72-73 0.3234030168277755 1102 74-75 0.2971757135140223 1102 76-77 0.3485877123421872 1102 78-79 0.3423804473028653 1102 80-81 0.32614975944819236 1102 82-83 0.3431177907609779 1102 84-85 0.35116441261077114 1102 86-87 0.32066473860118094 1102 88-89 0.3624719561495766 1102 90-91 0.27164196764813653 1102 92-93 0.29944514550255263 1102 94-95 0.382665343773823 1102 96-97 0.3403033316781716 1102 98-99 0.3011574906886807 1102 100 0.3245844295704501 1103 1 -0.12462596291006633 1103 2 -0.08478360299857712 1103 3 -0.04127899289438375 1103 4 -0.05575367325948832 1103 5 -0.04104395168412367 1103 6 0.03708033639674113 1103 7 0.053316526677669174 1103 8 0.05639026669143732 1103 9 0.12640573387646015 1103 10-11 0.1562188251483576 1103 12-13 0.05682881484378299 1103 14-15 0.20196208456547993 1103 16-17 0.184328009149354 1103 18-19 0.22505733496436875 1103 20-21 0.1878137767430701 1103 22-23 0.2244731071485404 1103 24-25 0.1895936561059628 1103 26-27 0.20324896974815942 1103 28-29 0.16792732364195473 1103 30-31 0.14166862958248316 1103 32-33 0.16066989287337918 1103 34-35 0.20971084414021846 1103 36-37 0.17853441323116215 1103 38-39 0.22261168390330965 1103 40-41 0.22636755285311239 1103 42-43 0.16904219579600976 1103 44-45 0.13902318249058965 1103 46-47 0.14181046436999623 1103 48-49 0.21919706767378955 1103 50-51 0.17809156921945402 1103 52-53 0.1648849780398649 1103 54-55 0.12760108255751845 1103 56-57 0.12831862499902513 1103 58-59 0.13478775104441354 1103 60-61 0.10653538477465219 1103 62-63 0.0958239191712309 1103 64-65 0.07504503083731606 1103 66-67 0.09518511714030353 1103 68-69 0.07910197027270272 1103 70-71 0.0674679228641466 1103 72-73 0.08482179952327584 1103 74-75 0.07763281101154007 1103 76-77 0.12709077035751903 1103 78-79 0.15094622519588086 1103 80-81 0.12120703036880798 1103 82-83 0.14151290095178837 1103 84-85 0.12535414551759771 1103 86-87 0.11718944637338069 1103 88-89 0.14880209309986014 1103 90-91 0.1553574172391876 1103 92-93 0.2035566500349475 1103 94-95 0.22298738950376418 1103 96-97 0.17955127330944265 1103 98-99 0.14631514707834725 1103 100 0.16590485278906897 1104 1 -0.008800638898534885 1104 2 0.02271344510431561 1104 3 0.04013368709061638 1104 4 0.043760924045976424 1104 5 0.0290155559196279 1104 6 0.07942103485705587 1104 7 0.14320346296439368 1104 8 0.11700236429594213 1104 9 0.1491781064678861 1104 10-11 0.18428243201803696 1104 12-13 0.11556181620947825 1104 14-15 0.20876290507912643 1104 16-17 0.23234716510859243 1104 18-19 0.22835320421641825 1104 20-21 0.2340408889914798 1104 22-23 0.27699305309342037 1104 24-25 0.20849871326159075 1104 26-27 0.25423259910289886 1104 28-29 0.24178532106321882 1104 30-31 0.20772877526566447 1104 32-33 0.19256244418575363 1104 34-35 0.22635259977964495 1104 36-37 0.23096841659071288 1104 38-39 0.2824281360789058 1104 40-41 0.24319709061060735 1104 42-43 0.3022742736160211 1104 44-45 0.2917586682932267 1104 46-47 0.18889303970115634 1104 48-49 0.14539875031017857 1104 50-51 0.18138123746952317 1104 52-53 0.2348482416895905 1104 54-55 0.24173195525497704 1104 56-57 0.15883810385068386 1104 58-59 0.17334886540483296 1104 60-61 0.17700265412489102 1104 62-63 0.1986231945713044 1104 64-65 0.1617011533544357 1104 66-67 0.15660037292612827 1104 68-69 0.12796494867633612 1104 70-71 0.14401976947851125 1104 72-73 0.16390814976026746 1104 74-75 0.11985471808750958 1104 76-77 0.14900234113369493 1104 78-79 0.14917900383918692 1104 80-81 0.13285877732683105 1104 82-83 0.17798327777456535 1104 84-85 0.1861933043681887 1104 86-87 0.16606252433795277 1104 88-89 0.18650366235697646 1104 90-91 0.22672517114820323 1104 92-93 0.25641943984005167 1104 94-95 0.288932917553943 1104 96-97 0.20323128546193914 1104 98-99 0.2088442257415437 1104 100 0.24042192682610164 1105 1 -0.17412071713498278 1105 2 -0.19692910566148214 1105 3 -0.13420257765837817 1105 4 -0.10285926434615078 1105 5 -0.09372133306823827 1105 6 1.1062483002177714E-4 1105 7 -0.02021732263539633 1105 8 -0.02263675154539868 1105 9 0.027785512459146844 1105 10-11 0.08567163311973758 1105 12-13 -0.0322594315119602 1105 14-15 0.10562634809894433 1105 16-17 0.169110564640377 1105 18-19 0.14193164269485692 1105 20-21 0.14617137200278307 1105 22-23 0.12653295354013494 1105 24-25 0.10158726708485943 1105 26-27 0.11232167114106772 1105 28-29 0.12723541346654343 1105 30-31 0.147140445535328 1105 32-33 0.07775302647022642 1105 34-35 0.11666913279589153 1105 36-37 0.0673528977350415 1105 38-39 0.0682648238033039 1105 40-41 0.09710716211726123 1105 42-43 0.05373209442430493 1105 44-45 0.07037890710627437 1105 46-47 0.024356568926727107 1105 48-49 0.0322804989213239 1105 50-51 0.02799657635055297 1105 52-53 0.07088522122499796 1105 54-55 0.11115345935272813 1105 56-57 0.10180155079212483 1105 58-59 0.08145161061327144 1105 60-61 0.07969045625644355 1105 62-63 0.03436843312172755 1105 64-65 0.04671444836787231 1105 66-67 0.04239643505309232 1105 68-69 0.05915607613689389 1105 70-71 0.06270479565886689 1105 72-73 0.02705739775341698 1105 74-75 0.0651898910837474 1105 76-77 0.11115036641978548 1105 78-79 0.05587116282751481 1105 80-81 0.11430017982645069 1105 82-83 0.08858284716599485 1105 84-85 0.08887248417405402 1105 86-87 0.10138446359616182 1105 88-89 0.08927885197810781 1105 90-91 0.11340312649629425 1105 92-93 0.16410651016812494 1105 94-95 0.1514712146857633 1105 96-97 0.11424280944093113 1105 98-99 0.10660397045767667 1105 100 0.14562172318164812 1106 1 -0.14425843607248368 1106 2 -0.1032356334180875 1106 3 -0.10641997928211566 1106 4 -0.08632555830378408 1106 5 -0.10002036868703357 1106 6 -0.007620983062189168 1106 7 0.008600999093836492 1106 8 0.024417405949790805 1106 9 0.08139441974020656 1106 10-11 0.07581002737506992 1106 12-13 0.03220958832728371 1106 14-15 0.10652964877059645 1106 16-17 0.08866264027180648 1106 18-19 0.04742879006705181 1106 20-21 0.04993823920092666 1106 22-23 0.0753473613882818 1106 24-25 0.03533151968087367 1106 26-27 0.07508785810583163 1106 28-29 0.005073868391427538 1106 30-31 0.04516128005889186 1106 32-33 0.0359579655908675 1106 34-35 0.030166961280393423 1106 36-37 0.021655141902954256 1106 38-39 0.10489871647689597 1106 40-41 0.09009031849583238 1106 42-43 0.049775967328614 1106 44-45 0.04335812063135336 1106 46-47 0.03196803486318345 1106 48-49 0.020092383369288314 1106 50-51 0.05207002124203797 1106 52-53 0.06569612637258615 1106 54-55 0.1164943542274699 1106 56-57 0.02951069350177704 1106 58-59 0.04319928677283258 1106 60-61 0.03910857887677821 1106 62-63 0.05452117295730119 1106 64-65 0.04310922123533345 1106 66-67 0.0195473028145301 1106 68-69 0.05555016538542645 1106 70-71 0.04999371163425792 1106 72-73 0.0388251527772141 1106 74-75 0.1057840613964629 1106 76-77 0.10416723579084675 1106 78-79 0.09613680135086611 1106 80-81 0.04212653876563621 1106 82-83 0.09091633965231694 1106 84-85 0.09243620994143242 1106 86-87 0.0987919202313634 1106 88-89 0.12523892427113736 1106 90-91 0.09937343516878627 1106 92-93 0.08809764896948735 1106 94-95 0.07385879103175341 1106 96-97 0.08430498441879308 1106 98-99 0.05923969412314989 1106 100 0.031569078535127915 1107 1 -0.09697898625855572 1107 2 -0.04452400557256908 1107 3 -0.013891616333495449 1107 4 0.03692780582285593 1107 5 0.012606383980724445 1107 6 0.09212151768440435 1107 7 0.10322719213788645 1107 8 0.14137988568550242 1107 9 0.12163945422953759 1107 10-11 0.13345476332689543 1107 12-13 0.06827986343962777 1107 14-15 0.19578827214596117 1107 16-17 0.008570778933766121 1107 18-19 0.07965154878011305 1107 20-21 0.10065919158395076 1107 22-23 0.06823768550556508 1107 24-25 0.07455046370032647 1107 26-27 0.13770525944133993 1107 28-29 0.09273639233845898 1107 30-31 0.08036214799373909 1107 32-33 0.0544616469699406 1107 34-35 0.12093967885556367 1107 36-37 0.10062081189842331 1107 38-39 0.09240801607445093 1107 40-41 0.07606507911138749 1107 42-43 0.09815809794362451 1107 44-45 0.12359219871017046 1107 46-47 0.1555108641488019 1107 48-49 0.09207985640095018 1107 50-51 0.15998361355251944 1107 52-53 0.10862386118391498 1107 54-55 0.13102441303149703 1107 56-57 0.1666659116984519 1107 58-59 0.15025163448990497 1107 60-61 0.1671477925131981 1107 62-63 0.15618269722154565 1107 64-65 0.09809672949257475 1107 66-67 0.09052646294793476 1107 68-69 0.07947628202769863 1107 70-71 0.15219301223032744 1107 72-73 0.15046922995868073 1107 74-75 0.14360957950135145 1107 76-77 0.1742110595934534 1107 78-79 0.1137429242178456 1107 80-81 0.10002919704565016 1107 82-83 0.19797859360406278 1107 84-85 0.18573543123550706 1107 86-87 0.15214365430298926 1107 88-89 0.18787769394882758 1107 90-91 0.14701496648243761 1107 92-93 0.1866123399499955 1107 94-95 0.23140894601048245 1107 96-97 0.12056035857984426 1107 98-99 0.14306349273563512 1107 100 0.19872318048885518 1108 1 -0.12737757679960282 1108 2 -0.10895117743254445 1108 3 -0.09707952428829003 1108 4 -0.029703684387520468 1108 5 -0.0857573846746007 1108 6 0.027397909840352952 1108 7 0.02748748033793902 1108 8 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2214 52-53 -0.1471400117010475 2214 54-55 -0.1491456044127375 2214 56-57 -0.13070201941928872 2214 58-59 -0.13840933389941767 2214 60-61 -0.12514007839494923 2214 62-63 -0.1010013518899413 2214 64-65 -0.10491800227122638 2214 66-67 -0.08999166829204341 2214 68-69 -0.0887232302965586 2214 70-71 -0.09210954648356307 2214 72-73 -0.10614424531605238 2214 74-75 -0.08729812669987069 2214 76-77 -0.11674391928567474 2214 78-79 -0.12744172737745885 2214 80-81 -0.1324681533507288 2214 82-83 -0.185057158833267 2214 84-85 -0.11696002288829277 2214 86-87 -0.13099798369667326 2214 88-89 -0.10687425337650325 2214 90-91 -0.09923786210477203 2214 92-93 -0.08484745993099096 2214 94-95 -0.13628330337773775 2214 96-97 -0.165372010994858 2214 98-99 -0.18594228613147123 2214 100 -0.17058459985924657 2215 1 -0.08434528026728572 2215 2 -0.2026065437015987 2215 3 -0.21572049095837542 2215 4 -0.10542272130251007 2215 5 -0.0655210575613765 2215 6 -0.19098874683488987 2215 7 -0.2436221371311902 2215 8 -0.29378476143281773 2215 9 -0.39817529156043463 2215 10-11 -0.4041870033262782 2215 12-13 -0.2966827068863154 2215 14-15 -0.4467280416428352 2215 16-17 -0.2653923493500514 2215 18-19 -0.3040699730728562 2215 20-21 -0.2963883384996535 2215 22-23 -0.3028886233515564 2215 24-25 -0.27704541396663984 2215 26-27 -0.2714681358771429 2215 28-29 -0.29150916781847513 2215 30-31 -0.2754361357522299 2215 32-33 -0.27625492491249304 2215 34-35 -0.2784377255274535 2215 36-37 -0.29596956622474124 2215 38-39 -0.25477669770916833 2215 40-41 -0.2783129655511587 2215 42-43 -0.25665385043459565 2215 44-45 -0.2748211657529396 2215 46-47 -0.2386451178532525 2215 48-49 -0.3362716258080525 2215 50-51 -0.22982885314722523 2215 52-53 -0.2504982546045369 2215 54-55 -0.26069078911008603 2215 56-57 -0.2652070126334678 2215 58-59 -0.31456529129941657 2215 60-61 -0.312377363993221 2215 62-63 -0.23266220420970996 2215 64-65 -0.23227978917043401 2215 66-67 -0.23606459356663834 2215 68-69 -0.3220848376077754 2215 70-71 -0.24896859965814144 2215 72-73 -0.2612928193016799 2215 74-75 -0.24665117814750914 2215 76-77 -0.220371870706785 2215 78-79 -0.20945236408706336 2215 80-81 -0.2058449102789126 2215 82-83 -0.2422400694770417 2215 84-85 -0.24021538386319463 2215 86-87 -0.226625229179092 2215 88-89 -0.2533443595648208 2215 90-91 -0.24331296334902675 2215 92-93 -0.30273243685541473 2215 94-95 -0.24707146867721974 2215 96-97 -0.2758846227339049 2215 98-99 -0.35907782305403657 2215 100 -0.4025000583041596 2216 1 -0.002670018271281549 2216 2 -0.07394495822468627 2216 3 -0.15815583821710533 2216 4 -0.14766474573892907 2216 5 -0.08473076640793664 2216 6 -0.10096510097151423 2216 7 -0.1571550857954236 2216 8 -0.3074454250243477 2216 9 -0.42109045781652554 2216 10-11 -0.30187501808616446 2216 12-13 -0.22901178497435382 2216 14-15 -0.3719418182175005 2216 16-17 -0.43384225335857707 2216 18-19 -0.38842845326573183 2216 20-21 -0.41569717866937594 2216 22-23 -0.3786353813786434 2216 24-25 -0.33682896329685974 2216 26-27 -0.38840753727971844 2216 28-29 -0.3589615538370552 2216 30-31 -0.3503866990230051 2216 32-33 -0.3474329224363899 2216 34-35 -0.4177245843441284 2216 36-37 -0.41550776244338294 2216 38-39 -0.3153625619541174 2216 40-41 -0.6625300352791612 2216 42-43 -0.8496256291976891 2216 44-45 -0.5411663521081067 2216 46-47 -0.4284496849100634 2216 48-49 -0.5295537728191704 2216 50-51 -0.48597808041358803 2216 52-53 -0.4547993467146725 2216 54-55 -0.5575981056393147 2216 56-57 -0.4314226243867978 2216 58-59 -0.5381640025266705 2216 60-61 -0.5740952774215202 2216 62-63 -0.5284005946159809 2216 64-65 -0.5652711799291055 2216 66-67 -0.5286821592058217 2216 68-69 -0.5906640126237264 2216 70-71 -0.5702650866501102 2216 72-73 -0.6187493870982408 2216 74-75 -0.500614587912807 2216 76-77 -0.5334827664885893 2216 78-79 -0.5871080447467136 2216 80-81 -0.5099774714164411 2216 82-83 -0.6886094477082239 2216 84-85 -0.5308568255487529 2216 86-87 -0.44384094036403354 2216 88-89 -0.4719742223984298 2216 90-91 -0.623362226788089 2216 92-93 -0.9755409784686186 2216 94-95 -0.8234732449794038 2216 96-97 -0.786324258889902 2216 98-99 -0.5912504430647942 2216 100 -0.520394151068821 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50827.0 3 8667.0 4 7345.0 5 6964.0 6 8472.0 7 11158.0 8 11637.0 9 11810.0 10 14035.0 11 13512.0 12 13827.0 13 14920.0 14 15793.0 15 16130.0 16 16745.0 17 17425.0 18 17268.0 19 16872.0 20 16767.0 21 17952.0 22 19268.0 23 22958.0 24 31272.0 25 47245.0 26 63006.0 27 68615.0 28 77278.0 29 85462.0 30 105332.0 31 134451.0 32 174103.0 33 233725.0 34 336615.0 35 559415.0 36 1185231.0 37 2545968.0 38 3436572.0 39 1257690.0 40 3945.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.93207824376068 12.158326460217793 11.286939034696417 50.622656261325105 2 20.511790626271914 19.687917050286753 36.858070888951396 22.942221434489937 3 25.33877373511692 22.46176708499912 23.721650922190765 28.477808257693194 4 27.16818903858394 29.467626161557526 18.595321804686765 24.76886299517177 5 28.352157367263427 31.05657829386919 21.062277895272995 19.528986443594384 6 22.67752564465961 33.542800426022 22.285809531253385 21.49386439806501 7 20.173026366757313 17.751573313971207 39.11544949363861 22.959950825632873 8 20.885546870469316 21.405539338009156 29.283853330515736 28.425060461005796 9 21.51390325961571 20.56833275494073 31.365741996274206 26.55202198916936 10-11 25.171211262492843 29.828510474920567 21.435641617684475 23.56463664490212 12-13 23.439328771032 24.081362786120273 27.181147471813787 25.29816097103394 14-15 23.95183981149344 25.207258990112834 26.420983149765693 24.419918048628038 16-17 24.814484188727413 25.322608391795104 25.28243241240157 24.58047500707592 18-19 24.570859106374996 25.41166281204616 25.206922909646686 24.810555171932155 20-21 24.44208639861627 25.59076092831736 25.514475641638047 24.452677031428323 22-23 24.60207416094404 25.666734322605894 25.47029681448975 24.260894701960318 24-25 24.38233415234104 25.774757890058382 25.49503906826646 24.347868889334112 26-27 24.199582335499255 25.871390336983225 25.591791104920443 24.337236222597078 28-29 24.376544500091885 25.499713171364487 25.46521239180114 24.658529936742493 30-31 24.26740438724347 25.592922033543346 25.493857055107465 24.64581652410572 32-33 24.20256132110266 25.840645301164134 25.592549631988774 24.364243745744428 34-35 24.57916135948915 25.66385975078657 25.257201901072374 24.499776988651906 36-37 24.37124760131865 25.561347880935557 25.386622955362405 24.680781562383387 38-39 24.284424072565717 25.720921706795387 25.546247723368843 24.448406497270053 40-41 24.501319383459375 25.597765998445805 25.430346919344338 24.470567698750486 42-43 24.297755253640783 25.45566084578933 25.629317674740953 24.617266225828928 44-45 24.30863028108547 25.634357565537314 25.720894412743476 24.33611774063374 46-47 24.427225780601287 25.63403599498757 25.409121102090925 24.529617122320218 48-49 24.30927524534226 25.497022775670125 25.57416359135195 24.619538387635668 50-51 24.32199803141778 25.631379376523306 25.67552229233624 24.371100299722677 52-53 24.438341323214065 25.674298219023772 25.36420851530427 24.523151942457886 54-55 24.25967842815554 25.55277824527365 25.525549080919756 24.661994245651055 56-57 24.27128863223085 25.63023464593525 25.660951897980922 24.43752482385298 58-59 24.47091166371776 25.59620025714305 25.470389385769877 24.462498693369316 60-61 24.296577533879514 25.587994436463518 25.58062250723479 24.53480552242218 62-63 24.189459728461323 25.787971585694454 25.709209423686918 24.313359262157302 64-65 24.384457786546673 25.650631728199297 25.46498752128547 24.499922963968558 66-67 24.308645508106203 25.57211789614597 25.56689718716527 24.552339408582554 68-69 24.327683332489713 25.740350395922334 25.65793148461356 24.27403478697439 70-71 24.416485060724106 25.638486558900063 25.445235982353903 24.49979239802193 72-73 24.28786600293606 25.636678878264462 25.536650558035745 24.53880456076373 74-75 24.268184676171504 25.845712396276866 25.557908343540635 24.32819458401099 76-77 24.487860271094558 25.629846011500806 25.470037394177066 24.41225632322757 78-79 24.324115854112314 25.50197697943902 25.6766564217641 24.497250744684564 80-81 24.27388328198352 25.749155780580857 25.711298869405177 24.26566206803045 82-83 24.387771804667597 25.637843282646905 25.524414397644424 24.449970515041073 84-85 24.332054137176282 25.535875810827967 25.67348888484748 24.45858116714827 86-87 24.309445232703084 25.80029613754308 25.70835908281196 24.181899546941878 88-89 24.421640398492865 25.724395813283863 25.454327211619255 24.399636576604024 90-91 24.30765572877506 25.6680884255521 25.526048214417465 24.49820763125538 92-93 24.230819410010707 25.855964381369528 25.703718113208364 24.20949809541141 94-95 24.54405421913724 25.73333676571426 25.41182252406372 24.31078649108478 96-97 24.405463590284672 25.692194845601335 25.53296312880115 24.369378435312843 98-99 24.414151397354146 25.852032931113182 25.569249843824604 24.164565827708067 100 24.637743318851243 25.607544445940704 25.2068225453103 24.547889689897755 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 38.0 1 27.5 2 21.5 3 39.0 4 63.0 5 98.0 6 153.5 7 212.0 8 309.5 9 401.5 10 489.5 11 628.0 12 747.0 13 864.5 14 992.0 15 1137.0 16 1406.5 17 1843.5 18 2258.0 19 2976.0 20 4358.0 21 6472.5 22 9959.0 23 14990.5 24 21326.5 25 29608.0 26 40661.0 27 53350.0 28 66039.0 29 79053.0 30 93147.5 31 108507.5 32 123809.0 33 139510.5 34 156334.0 35 175240.5 36 197203.5 37 219475.5 38 238335.0 39 255262.0 40 270416.0 41 284941.5 42 301136.0 43 317410.0 44 327314.0 45 337505.5 46 351155.0 47 356693.0 48 359459.0 49 359595.5 50 361507.5 51 364582.0 52 360097.5 53 353042.5 54 345294.0 55 337835.5 56 321799.0 57 301918.5 58 289764.0 59 281334.5 60 272734.5 61 257301.0 62 236874.0 63 218612.0 64 200653.5 65 178462.0 66 152918.5 67 130367.0 68 111064.0 69 89993.5 70 67762.5 71 49343.5 72 35813.0 73 24638.0 74 15996.5 75 9759.5 76 5664.0 77 3216.0 78 1850.5 79 1011.0 80 581.5 81 384.0 82 255.0 83 181.5 84 139.0 85 108.5 86 82.0 87 60.0 88 50.0 89 37.0 90 36.5 91 34.0 92 23.0 93 19.5 94 19.5 95 17.5 96 18.5 97 13.0 98 8.0 99 9.5 100 20.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6802273351746594 2 0.5172266948584072 3 0.5110282764741414 4 0.49975332538601985 5 0.5158991301365886 6 0.7208769929948523 7 0.8028120438541373 8 0.8555126236914021 9 0.8887017417368679 10-11 0.38213296084235665 12-13 0.6235814573612856 14-15 0.44429477658441346 16-17 0.031043511681681395 18-19 2.1502808874527087E-4 20-21 0.026785020619791353 22-23 0.0 24-25 0.0 26-27 0.005712267922754805 28-29 0.008100949517294663 30-31 0.0024961956389124925 32-33 0.0 34-35 0.03735879315765663 36-37 3.3189118045465725E-4 38-39 0.008633845215489464 40-41 0.1150820981917353 42-43 0.09591187662772757 44-45 0.07260937614087593 46-47 0.0416500058852253 48-49 0.1780993517651048 50-51 0.08786234687078505 52-53 0.0725205601911768 54-55 0.12040638065001494 56-57 0.0792051290369537 58-59 0.09719269611286245 60-61 0.12960584322937785 62-63 0.15572240696459153 64-65 0.06052573245812538 66-67 0.07547953367325846 68-69 0.07662479197201046 70-71 0.036849270077803704 72-73 0.0729599654160041 74-75 0.17644924491016828 76-77 0.15837753640822877 78-79 0.12682917617036282 80-81 0.1550913462693608 82-83 0.10441016065683416 84-85 0.19345516201571816 86-87 0.19160405064304148 88-89 0.11003361262988981 90-91 0.08566064622298021 92-93 0.17920253935084143 94-95 0.12810064660816095 96-97 0.17535073184810004 98-99 0.19427320365768388 100 0.16640369354682943 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0696277E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.19754323769334 #Duplication Level Percentage of deduplicated Percentage of total 1 80.38705430569716 47.5871612301231 2 10.70966375090258 12.679715659104252 3 3.4405507994433195 6.110164641345789 4 1.668716315414492 3.9513562493277483 5 0.9200356937983524 2.723192638192458 6 0.5753451393327128 2.043541125734698 7 0.38931431792795623 1.6132515818015298 8 0.28170321941096477 1.33409108090224 9 0.21764657619664246 1.1595728344442995 >10 1.2866836337136107 14.154641672178151 >50 0.0918565537647529 3.6893892086528837 >100 0.03117588470199632 2.86641670689285 >500 2.538096955232939E-4 0.0875053713000157 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.005291560792601014 9.34904733675091E-6 0.0 0.0 0.0 2 0.005357004123958271 6.544333135725636E-5 0.0 0.0 0.0 3 0.005422447455315527 6.544333135725636E-5 0.0 0.0 0.0 4 0.005777711254112062 7.479237869400728E-5 0.0 9.34904733675091E-6 9.34904733675091E-6 5 0.005824456490795816 7.479237869400728E-5 0.0 1.869809467350182E-5 9.34904733675091E-6 6 0.006114276958235095 7.479237869400728E-5 0.0 3.739618934700364E-5 9.34904733675091E-6 7 0.0061703712422556 8.414142603075818E-5 0.0 3.739618934700364E-5 9.34904733675091E-6 8 0.006385399331000871 8.414142603075818E-5 0.0 3.739618934700364E-5 9.34904733675091E-6 9 0.006544333135725636 9.349047336750909E-5 0.0 4.6745236683754546E-5 9.34904733675091E-6 10-11 0.007128648594272568 9.349047336750909E-5 0.0 4.6745236683754546E-5 9.34904733675091E-6 12-13 0.007848525239202387 9.816499703588455E-5 0.0 1.0283952070425999E-4 1.4023571005126365E-5 14-15 0.008890944017250114 1.3556118638288818E-4 0.0 1.0751404437263545E-4 1.869809467350182E-5 16-17 0.009998806126655097 1.4958475738801455E-4 0.0 1.1686309170938635E-4 1.869809467350182E-5 18-19 0.011405837750836109 1.5893380472476544E-4 0.0 1.3088666271451272E-4 1.869809467350182E-5 20-21 0.01295777960873676 1.6828285206151635E-4 0.0 1.3088666271451272E-4 1.869809467350182E-5 22-23 0.014346113138244269 1.8698094673501818E-4 0.0 1.3088666271451272E-4 1.869809467350182E-5 24-25 0.01578586642810391 2.150280887452709E-4 0.0 1.3556118638288818E-4 1.869809467350182E-5 26-27 0.017150827339269543 2.3372618341877273E-4 0.0 1.4023571005126364E-4 1.869809467350182E-5 28-29 0.01888040109656846 2.4307523075552362E-4 0.0 1.4023571005126364E-4 2.8047142010252727E-5 30-31 0.020521158904168245 2.5242427809227453E-4 0.0 1.5893380472476544E-4 2.8047142010252727E-5 32-33 0.02224138361413041 2.5242427809227453E-4 0.0 1.6828285206151635E-4 2.8047142010252727E-5 34-35 0.023924212134745576 2.7112237276577636E-4 0.0 1.6828285206151635E-4 2.8047142010252727E-5 36-37 0.02573325279440688 2.8514594377090273E-4 0.0 1.729573757298918E-4 2.8047142010252727E-5 38-39 0.027551642501404926 2.991695147760291E-4 0.0 1.7763189939826727E-4 2.8047142010252727E-5 40-41 0.029416777445086736 2.991695147760291E-4 0.0 1.8698094673501818E-4 2.8047142010252727E-5 42-43 0.031473567859171936 3.0851856211277996E-4 0.0 1.9165547040339364E-4 2.8047142010252727E-5 44-45 0.03350698565491526 3.0851856211277996E-4 0.0 1.9632999407176907E-4 2.8047142010252727E-5 46-47 0.0356712901133731 3.178676094495309E-4 0.0 2.0100451774014453E-4 2.8047142010252727E-5 48-49 0.037807547429820676 3.3189118045465725E-4 0.0 2.0567904140851998E-4 2.8047142010252727E-5 50-51 0.03995782831727338 3.365657041230327E-4 0.0 2.0567904140851998E-4 2.8047142010252727E-5 52-53 0.04234650991181324 3.459147514597836E-4 0.0 2.2905165975039727E-4 2.8047142010252727E-5 54-55 0.04483335650338899 3.5993832246491E-4 0.0 2.4307523075552362E-4 2.8047142010252727E-5 56-57 0.047535231183709996 3.6461284613328545E-4 0.0 2.5242427809227453E-4 2.8047142010252727E-5 58-59 0.05047083204744978 3.6461284613328545E-4 0.0 2.5709880176065E-4 2.8047142010252727E-5 60-61 0.05342513100586307 3.692873698016609E-4 0.0 2.6177332542902545E-4 2.8047142010252727E-5 62-63 0.056645877813373754 3.786364171384118E-4 0.0 2.757968964341518E-4 2.8047142010252727E-5 64-65 0.060151770564655344 3.833109408067873E-4 0.0 2.8514594377090273E-4 2.8047142010252727E-5 66-67 0.06388204045201896 3.833109408067873E-4 0.0 2.898204674392782E-4 2.8047142010252727E-5 68-69 0.06823402198727652 3.8798546447516274E-4 0.0 2.898204674392782E-4 2.8047142010252727E-5 70-71 0.07343676683017838 4.207071301537909E-4 0.0 2.898204674392782E-4 3.739618934700364E-5 72-73 0.0796258361671075 4.207071301537909E-4 0.0 2.898204674392782E-4 3.739618934700364E-5 74-75 0.08672176309570143 4.207071301537909E-4 0.0 3.178676094495309E-4 3.739618934700364E-5 76-77 0.09502371713073623 4.2538165382216634E-4 0.0 3.365657041230327E-4 3.739618934700364E-5 78-79 0.10531701824849898 4.394052248272927E-4 0.0 3.459147514597836E-4 3.739618934700364E-5 80-81 0.11751285049929056 4.4407974849566817E-4 0.0 3.6461284613328545E-4 3.739618934700364E-5 82-83 0.1342055745190593 4.4875427216404363E-4 0.0 3.6461284613328545E-4 3.739618934700364E-5 84-85 0.15704062263907337 4.534287958324191E-4 0.0 3.6461284613328545E-4 3.739618934700364E-5 86-87 0.18728946529713097 4.5810331950079454E-4 0.0 4.2538165382216634E-4 3.739618934700364E-5 88 0.2149345982719034 4.5810331950079454E-4 0.0 4.394052248272927E-4 3.739618934700364E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGAAT 380 2.421653E-4 11.212382 1 GTCCGAT 1045 0.0 10.872613 1 CTCGTAT 1310 0.0 10.480106 1 GATCGGA 5280 0.0 9.87292 94 CTCGACT 1640 0.0 9.814633 1 CGTATCG 925 6.9485395E-10 9.721057 3 CGGGGTT 3285 0.0 9.511467 1 CGCGAGT 855 1.9395884E-8 9.412864 1 CTCGCGA 455 0.0012714139 9.364186 1 CTCCGGT 2610 0.0 9.250573 1 CGACGAT 1030 4.965841E-10 9.19246 1 CGCGGTT 1275 3.6379788E-12 8.911278 1 CGCATAT 800 7.087692E-7 8.87647 1 CCCGTAT 1550 0.0 8.85738 1 CTCGGTT 2245 0.0 8.8567 1 CGCCATT 2940 0.0 8.85634 1 CTGGAAT 11330 0.0 8.732837 1 CTCGCAT 2495 0.0 8.728231 1 CTCAGAT 8460 0.0 8.61766 1 CGCGTTT 730 1.9236277E-5 8.430619 1 >>END_MODULE