FastQCFastQC Report
Wed 25 May 2016
SRR1033282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033282_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10696277
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT7552.1827873E-1011.2618351
TCCGATT18850.011.0220832
GTCCGAT10200.010.6515121
CTCGCAT24550.010.1978351
CTCCGAT22100.010.0459071
CTCGGTT20500.09.90827751
CGCGAGT7653.630339E-89.8796631
CGCGTTT6951.037346E-69.5153951
GATCGGA55800.09.51425294
CGCTAGG5507.2730276E-59.4474291
GTTCGAT13550.09.4125661
CCGGATT26850.08.9724181
CGGGGTT30200.08.9156191
CCGGGAT32500.08.8660471
CTCAGAT87900.08.7595611
CGCGGAT11403.8016879E-108.7015781
CGCGTAA3854.4778717E-68.5421670-71
CTCGGAT32400.08.4560321
CCCGTAT14105.456968E-128.3753791
CTCGAAT24500.08.29061
Produced by FastQC (version 0.11.5)