FastQCFastQC Report
Wed 25 May 2016
SRR1033281_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033281_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3900294
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN153710.3940984961646481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58850.042.41531
GTATCAA103250.030.1691111
GTGGTAT26200.026.9363841
TGGTATC26600.023.9139752
ATCAACG135850.023.3914433
TCAACGC137550.023.0993674
CAACGCA139550.022.8178885
AACGCAG144500.022.2031046
TATCAAC140000.022.0479012
ACGCAGA162750.019.439437
CGCAGAG163600.019.2333328
CGATACG1108.1745046E-418.84105526
GTACATG97800.018.7333871
AGAGTAC162800.018.08226611
GCAGAGT173800.017.6699459
CAGAGTA172650.017.54861510
GAGTACT121950.017.42770412
TACATGG96800.017.2723752
AGTACTT124950.016.7617813
ACATGGG102750.016.36713
Produced by FastQC (version 0.11.5)