FastQCFastQC Report
Wed 25 May 2016
SRR1033281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033281_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3900294
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58400.043.669891
GTATCAA109650.031.0116651
GTGGTAT27150.026.0929161
TGGTATC25850.024.4107782
TCAACGC140250.023.9221954
ATCAACG140450.023.839033
CAACGCA143000.023.4621545
TATCAAC145850.023.0746152
AACGCAG150100.022.4215566
ACGCAGA172400.019.4413177
CGCAGAG173000.019.3340268
CGCGGTA2002.8177965E-819.01452458
AGAGTAC173500.018.26470611
GCAGAGT183450.017.7439779
GAGTACT130600.017.74282512
CAGAGTA181550.017.70175410
GTACATG103400.017.2451821
AGTACTT135900.016.8984113
GTACTTT139550.016.50584214
TACATGG103350.016.315092
Produced by FastQC (version 0.11.5)