FastQCFastQC Report
Wed 25 May 2016
SRR1033280_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033280_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3615937
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN140070.3873684746166761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53950.041.34851
GTATCAA99500.030.8706191
ATCAACG124800.025.1578873
TCAACGC125500.025.040444
CAACGCA128650.024.4276645
TATCAAC127600.024.0141332
GTGGTAT24300.023.879561
AACGCAG132950.023.7678996
TGGTATC24600.022.1724452
ACGCAGA151100.020.6867947
CGCAGAG153800.020.2118438
GAGTACT110750.020.12042612
TCGTACG1055.7497463E-419.8523042
AGAGTAC153450.019.27247211
AGTACTT117500.018.6711313
GTCTCGC5400.018.6603761
CAGAGTA161650.018.61393510
GCAGAGT164200.018.4924059
CGTACGC950.00798474418.0994073
GTACTTT123200.017.95308114
Produced by FastQC (version 0.11.5)