FastQCFastQC Report
Wed 25 May 2016
SRR1033280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3615937
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60050.041.7604981
GTATCAA106100.029.7233311
GTGGTAT26350.026.4796871
ATCAACG130750.023.655033
TCAACGC131700.023.5105764
TGGTATC27300.023.3658622
TATCAAC135700.022.9446052
CAACGCA135300.022.834055
AACGCAG140100.022.1255596
TATACCG1756.2536856E-921.6734165
ACGCAGA160800.019.1915367
CGCAGAG162400.018.9175288
GAGTACT116950.018.51561412
CGAACTA2105.0786184E-818.06117829
AGAGTAC160450.018.00872811
AGTACTT123150.017.44344713
GCAGAGT173500.017.4290149
CAGAGTA171000.017.34127810
GCGAACT2601.7862476E-917.24021728
GTACTTT127200.016.88805614
Produced by FastQC (version 0.11.5)