FastQCFastQC Report
Wed 25 May 2016
SRR1033279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378264
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN93750.39419509356404503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41150.040.0501631
GTATCAA71850.027.9702841
GTGGTAT17650.025.6091061
ATCAACG87750.022.9771773
TCAACGC88600.022.7902744
CAACGCA89800.022.3325045
TGGTATC18550.022.1136132
TATCAAC89900.021.925272
AACGCAG93450.021.6083856
TAACGGC1258.725509E-519.32590136
ACGCAGA105900.018.8747167
CGCAGAG105850.018.851138
AGAGTAC102800.017.9398111
GAGTACT75750.017.44193812
CTTAACG1402.0795646E-417.25453858
GCAGAGT112450.017.0734339
GTACATG58650.017.0141281
CAGAGTA110800.016.9550510
GCGAACT1855.406455E-616.79852728
TACATGG58500.016.520022
Produced by FastQC (version 0.11.5)