FastQCFastQC Report
Wed 25 May 2016
SRR1033279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378264
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42650.039.367981
CGTTCGT450.00463635530.64950434
GTATCAA72300.026.7593541
ACCGGTC700.001338625224.6290668
GTGGTAT19350.024.1034221
TGGTATC18850.023.7797872
ATCAACG89800.021.310453
TAAGCGC1304.9494174E-621.21888523
TCAACGC90150.021.1512174
CAACGCA92750.020.5582985
TATCAAC94200.020.3150562
ACGAACG850.00413483820.28275952
AACGCAG95550.020.1002036
CGAACTA1601.370403E-619.39538829
CGAACGA900.005754766519.1559453
CGCGGTA1501.7033493E-518.389743
TCGCCCC2156.642949E-817.64128338
ACGCAGA109800.017.3031527
GTACATG59650.017.2596421
CGCAGAG109300.017.2245718
Produced by FastQC (version 0.11.5)