FastQCFastQC Report
Wed 25 May 2016
SRR1033278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3753376
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN152780.4070468825931641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55000.042.335111
GTATCAA99750.029.857711
GTGGTAT22950.025.2884581
ATCAACG121750.024.2125113
TCAACGC123000.023.9915244
CAACGCA125500.023.5690485
TATCAAC123550.023.5396922
AACGCAG130000.022.8067196
TGGTATC24350.022.260952
CCGATCG1507.552917E-720.638839
CGATCGC1056.148338E-419.65576610
ACGCAGA150100.019.5472057
CGCAGAG152000.019.1447188
GTACATG91900.018.000311
AGAGTAC150700.017.98626311
GAGTACT107750.017.65370612
GCAGAGT162300.017.4215649
CAGAGTA159700.017.2957210
TACATGG93350.017.1967242
ACATGGG91500.017.1048133
Produced by FastQC (version 0.11.5)