FastQCFastQC Report
Wed 25 May 2016
SRR1033278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3753376
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53550.042.7730141
GTATCAA94800.028.8624131
CGCGTAT854.4491517E-628.39738712
GTGGTAT24250.026.0708481
TGGTATC23850.024.8672492
TATACCG1401.3978934E-824.629735
ATCAACG117450.023.0464653
TCAACGC118950.022.7558424
CAACGCA121750.022.2608265
TATCAAC123100.021.9886882
AACGCAG125600.021.605926
ACGCAGA147900.018.2316637
CGCAGAG149750.017.9373518
TATAACG2902.1827873E-1117.8353212
GTACATG93300.017.736571
ATACCGT3350.017.4981376
GAGTACT105550.017.41278512
CGCGGTA1804.2226857E-617.24081243
AGAGTAC149550.016.99340411
TACATGG95050.016.5061952
Produced by FastQC (version 0.11.5)