FastQCFastQC Report
Wed 25 May 2016
SRR1033277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4432580
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN176700.39863916725699255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60550.040.1266171
GTATCAA110150.031.8086741
CGCGTAT802.8333616E-630.09164612
GTGGTAT27100.024.7546921
ATCAACG144050.024.6865253
TCAACGC145300.024.3978824
CAACGCA149050.023.8073865
TATCAAC148400.023.4425562
AACGCAG153050.023.253636
TGGTATC28100.022.8807472
ACGCAGA177100.019.8059147
TAGACCG1751.5281148E-719.6483445
CGCAGAG178900.019.3951938
GTACATG130000.019.1710241
TACATGG130300.018.5105722
AGAGTAC179100.018.35693711
GAGTACT123650.018.18953112
CAGAGTA184750.017.8886210
GCAGAGT190050.017.842149
ACATGGG136300.017.8392353
Produced by FastQC (version 0.11.5)