FastQCFastQC Report
Wed 25 May 2016
SRR1033277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4432580
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67600.040.7906381
GTATCAA118350.029.868381
GTGGTAT29550.027.713741
TGGTATC31000.024.4705282
ATCAACG150600.023.2622153
TCAACGC151850.023.0480184
CAACGCA155450.022.5586195
TATCAAC156050.022.5381742
AACGCAG161600.021.7854586
GTACATG131450.019.3736381
ACGCAGA187400.018.6021827
TACATGG129800.018.3828872
CGCAGAG190350.018.2233168
TCGCGTG950.00786154218.14840912
AGAGTAC188350.017.37371611
ACATGGG134200.017.2405973
GAGTACT128300.017.22754912
CAGAGTA194250.017.09434510
GCAGAGT201000.016.8462289
AGTACTT132200.016.69324113
Produced by FastQC (version 0.11.5)